Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,467 G→T 5.4% intergenic (‑327/+37) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4670GT 5.4% 371.5 / 6.8 126intergenic (‑327/+37)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base G (63/56);  new base T (4/3);  total (67/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 129 total aligned reads displayed.

TTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTA  >  NC_000913/1567318‑1567615
                                                                                                                                                     |                                                                                                                                                    
ttattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTggg                                                                                                                                                      >  1:1699897/1‑150 (MQ=255)
ttattTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTggg                                                                                                                                                      >  2:1845898/1‑150 (MQ=255)
          gTGACAGCGAATATCGTATGGTGTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGTTGGGTAAAGCACCg                                                                                                                                            >  2:1307121/1‑150 (MQ=255)
                gctaaTATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCGGGTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                      <  2:1067794/147‑1 (MQ=255)
                 ctaaTAACTTATTGCTTTTCATAATCCAACATGAATCTTAGTGAGATATTGCTGTTTACCTAACTCTCGTTAATTTGACTTAATTAACCATGATTATTAGTTGGATAGTTTACGCGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                     <  2:353516/148‑1 (MQ=255)
                     tatCGTATGGTTTTTCATATTCATACATATTTATTAGGGAATTATGGCTGATTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATACTCTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaa                                                                                                                                 <  2:148736/150‑1 (MQ=255)
                      atCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                >  1:1873067/1‑150 (MQ=255)
                       tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCAGGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAg                                                                                                                               <  1:1275242/150‑1 (MQ=255)
                        cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctct                                                                                                                              <  2:489580/150‑6 (MQ=38)
                          tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctctta                                                                                                                            <  1:1243265/150‑8 (MQ=38)
                           aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                           <  1:894458/150‑1 (MQ=255)
                              gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAAt                                                                                                                        >  2:1181422/1‑150 (MQ=255)
                               tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                       <  1:1483171/150‑1 (MQ=255)
                               tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                       <  1:169104/150‑1 (MQ=255)
                                 tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                     <  2:658457/150‑1 (MQ=255)
                                  ttcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                    <  2:1668506/150‑1 (MQ=255)
                                    aatattcataCATTTTTATTAGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                 <  1:995921/149‑1 (MQ=255)
                                       attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCATGTTACAAAGTAAGAATGGGAGTTTa                                                                                                               >  2:1998824/1‑150 (MQ=255)
                                         tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                             >  1:1951691/1‑150 (MQ=255)
                                         tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                             <  1:687451/150‑1 (MQ=255)
                                          cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                            >  2:1373722/1‑150 (MQ=255)
                                           ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACGAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTg                                                                                                           >  1:1536759/1‑150 (MQ=255)
                                             aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                         <  2:173943/150‑1 (MQ=255)
                                              cATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                        >  1:1636295/1‑150 (MQ=255)
                                               aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGAGTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcccc                                                                                                       >  2:1005162/1‑150 (MQ=255)
                                                tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACAGGCTTGTTACATATTAAGAATGGGAGTTTAACTGcccaa                                                                                                      >  1:286860/1‑148 (MQ=255)
                                                 ttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAg                                                                                                     <  1:799484/150‑1 (MQ=255)
                                                   ttattaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTTAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCg                                                                                                   <  1:45383/150‑1 (MQ=255)
                                                     attaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGACGGTGGGTAAAGAAACGTCTTGTTACAAAGTAAGAATGTGAGTTTAACTGCcacatctaa                                                                                                 >  1:143953/1‑145 (MQ=255)
                                                       taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                               >  1:79607/1‑150 (MQ=255)
                                                       taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                               >  2:1549530/1‑150 (MQ=255)
                                                         gggATTTATGGCTCTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAACGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                             >  2:1660318/1‑150 (MQ=255)
                                                          ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTCAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                            <  2:1343125/150‑1 (MQ=255)
                                                          ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                            <  1:543724/150‑1 (MQ=255)
                                                          ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                            <  1:1645776/150‑1 (MQ=255)
                                                           gATTTCTGGCTCTCTAACTAAGTGTGGCTAATTTGACTCAACTAAGCATGATTATTAGTCGGACAGGCTCCGAGGGTAACACGCCGGGGGGGAAAGCACCGGCTTGTTACAAAGTAAGAATGCGCGTTTAACTGCCCCAGCGACTTTGCa                                                                                           <  2:1448122/150‑1 (MQ=255)
                                                            aTTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAg                                                                                          <  2:1873067/150‑1 (MQ=255)
                                                               tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCTCCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                       >  2:424486/1‑150 (MQ=255)
                                                               tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                       >  2:859693/1‑150 (MQ=255)
                                                               tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                       <  1:1747218/150‑1 (MQ=255)
                                                                 ttgatgtgTAACTAAGTTTGTTTATTTTGTCTTAATTAATCATGATTATTAGTTGGATAGTTTAAGATGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                     <  1:270184/146‑1 (MQ=255)
                                                                  ggCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGAAGATAgat                                                                                    >  2:1329429/1‑148 (MQ=255)
                                                                         tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                             <  1:1029682/150‑1 (MQ=255)
                                                                         tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                             >  1:1582908/1‑150 (MQ=255)
                                                                         tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACAGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACAGCt                                                                             >  1:1684136/1‑150 (MQ=255)
                                                                          aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                            <  2:2099812/150‑1 (MQ=255)
                                                                           aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                           >  1:727241/1‑150 (MQ=255)
                                                                              aaGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgttg                                                                        <  1:1660963/150‑1 (MQ=255)
                                                                              aaGTGTGGTCAATATGACTTAATTAAGCCTGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGCTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAACCGGAGTTTAACTGCCCCAGCGACGTTGCAGATACCTGACCGCttgtag                                                                        <  2:1497769/150‑3 (MQ=255)
                                                                              aaGTGTCGCTAATTTGACTTAAGTAAGCCTCCTTATTAGTGGGATAGTTTCAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTCAGAATGGGAGTTTAACTGCCCCAGCGACTTGGCAGATAGCGGACCGCttgttg                                                                        <  2:211003/150‑1 (MQ=255)
                                                                               aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGTCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                       >  1:158068/1‑150 (MQ=255)
                                                                                gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                      <  2:513504/150‑1 (MQ=255)
                                                                                gtgtGGGTTATTAGACTTAAGTAAGCATGATTATTAGTGGCAAAGTATAATAGGGAAACAAGCCGGTGGGTAAAGCACGGGCTTGTGACAAAGTAAGAATGGGAGTGTAACTGCCCCAGGGACTTAGCAGATAGCATACCGCgtggggag                                                                      >  2:84394/1‑145 (MQ=255)
                                                                                    ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                  >  1:1493420/1‑150 (MQ=255)
                                                                                    ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                  >  1:1500541/1‑150 (MQ=255)
                                                                                    ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                  >  1:719164/1‑150 (MQ=255)
                                                                                      ttAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTAGTTGAGTCTGCg                                                                >  1:929194/1‑150 (MQ=255)
                                                                                       tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                               <  2:1656313/150‑1 (MQ=255)
                                                                                       tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                               <  1:1856497/150‑1 (MQ=255)
                                                                                         aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCAGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                             <  1:1325100/150‑1 (MQ=255)
                                                                                           ttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTa                                                           <  2:477447/150‑1 (MQ=255)
                                                                                               cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTTCAAAGTAAGAATGGGAGTTTAACTGACCCAGCGACTTTGCAGATAGCTGACAGCTTGTTGAGTCTGCGGTTTTTTCa                                                       >  1:1256959/1‑150 (MQ=255)
                                                                                               cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCa                                                       >  1:1508678/1‑150 (MQ=255)
                                                                                                ttAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAg                                                      >  1:2067534/1‑150 (MQ=255)
                                                                                                  aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                    >  1:1332523/1‑150 (MQ=255)
                                                                                                  aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                    <  2:160567/150‑1 (MQ=255)
                                                                                                     taagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGt                                                 <  1:781624/150‑1 (MQ=255)
                                                                                                        gCATGATTCTTAGGGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGACTGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGGCTGCGGTTTATTCAGATAGTCCt                                              <  2:302251/150‑1 (MQ=255)
                                                                                                        gCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTAGAGTCTGCGGTTTATTCAGATAGTCCt                                              >  2:1521957/1‑150 (MQ=255)
                                                                                                         cATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTc                                             >  2:770332/1‑150 (MQ=255)
                                                                                                            gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTTTGTAAAGCACCTGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                          <  1:1143126/150‑1 (MQ=255)
                                                                                                            gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCACACg                                          >  2:1248894/1‑150 (MQ=255)
                                                                                                             attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACgg                                         >  1:953679/1‑150 (MQ=255)
                                                                                                              ttattaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGa                                        >  2:598390/1‑150 (MQ=255)
                                                                                                                attaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                      <  2:732073/150‑1 (MQ=255)
                                                                                                                    gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGa                                  <  1:1181422/150‑1 (MQ=255)
                                                                                                                     tGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGTGAGTTTAACTGCCCCAGCGACTTAGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTAATTCAGATAGTCCTCACGGAACAAGAt                                 >  2:2051600/1‑150 (MQ=255)
                                                                                                                     tGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGAt                                 >  1:883939/1‑150 (MQ=255)
                                                                                                                      gggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                >  1:34175/1‑150 (MQ=255)
                                                                                                                       ggATAGTTAAAGCGGGAAACAAGCCGGGGGGGAACGCACCGGCTTGTTACAAAGTAAGCATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTGGTGGAGTCTGCGGTTTATTCAGATACTCCTCACGGAACAAGATgg                               <  2:1394940/150‑1 (MQ=255)
                                                                                                                       ggATAGTATAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGCGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTTTTGAGTCTGCGGTTAATTCAAATAGTCCTCACGTAACAAGATgg                               >  1:1654550/1‑150 (MQ=255)
                                                                                                                           aGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGcc                           >  1:2028584/1‑150 (MQ=255)
                                                                                                                            gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTAGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                          >  1:580131/1‑150 (MQ=255)
                                                                                                                               tAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTa                       >  2:1409242/1‑150 (MQ=255)
                                                                                                                               aaaGACGGTAACAACCCGCTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGGTCCACTGCCCCAGCGACTTTGCAGATAGCTGCCCGCTCGTCGACGCTGCGATTTATTCAGATAGTCCTCACCGACCAAGATCGGGCCGCTa                       <  2:1484743/149‑1 (MQ=255)
                                                                                                                                 agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAAt                     >  2:1140137/1‑150 (MQ=255)
                                                                                                                                  gagGGTAACAAGCCGGTGGGTAAAGCAGCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGATGGTGCCGCTAAtt                    >  1:141564/1‑150 (MQ=255)
                                                                                                                                    gggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTGACGGAACAAGATGGTGCGGCTAATTTc                  >  1:1346059/1‑150 (MQ=255)
                                                                                                                                    gggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACCGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTc                  >  1:1040872/1‑150 (MQ=255)
                                                                                                                                     ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                 >  2:946902/1‑150 (MQ=255)
                                                                                                                                      gTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                <  1:1375367/150‑1 (MQ=255)
                                                                                                                                       tAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg               >  2:1393529/1‑150 (MQ=255)
                                                                                                                                          cAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTATGTATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCTCGGAACAAGATGGTGCCGCTAATTTCGGGcac            <  1:126791/150‑1 (MQ=255)
                                                                                                                                          cAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCATATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac            >  1:854500/1‑150 (MQ=255)
                                                                                                                                          cAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac            >  1:2080766/1‑150 (MQ=255)
                                                                                                                                          cAAGCCGGGGCGCAAAGCACCGGCTTGTTACAAAGTAACAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGGTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac            <  2:1527280/150‑1 (MQ=255)
                                                                                                                                           aaGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCTGATAGTCCTCACGGAACACGATGGTGCCGCTAATTTCGGGcaca           >  2:1012094/1‑150 (MQ=255)
                                                                                                                                            aGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg          <  1:532338/150‑1 (MQ=255)
                                                                                                                                                ggTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATc      >  2:1490584/1‑150 (MQ=255)
                                                                                                                                                    ggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTa  <  2:1396295/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTA  >  NC_000913/1567318‑1567615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: