Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,471 A→C 5.9% intergenic (‑331/+33) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4710AC 5.9% 376.6 / 10.4 128intergenic (‑331/+33)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base A (63/57);  new base C (4/4);  total (67/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 128 total aligned reads displayed.

GTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAG  >  NC_000913/1567328‑1567617
                                                                                                                                               |                                                                                                                                                  
gTGACAGCGAATATCGTATGGTGTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGTTGGGTAAAGCACCg                                                                                                                                              >  2:1307121/1‑150 (MQ=255)
      tcGAATATCGTATGGGTTTTCATATTCATAAATTTTTATTAGTGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                        <  1:610937/149‑1 (MQ=255)
      gctaaTATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCGGGTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                        <  2:1067794/147‑1 (MQ=255)
       ctaaTAACTTATTGCTTTTCATAATCCAACATGAATCTTAGTGAGATATTGCTGTTTACCTAACTCTCGTTAATTTGACTTAATTAACCATGATTATTAGTTGGATAGTTTACGCGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                       <  2:353516/148‑1 (MQ=255)
        gAATATCGAATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa                                                                                                                                      <  1:1995893/150‑1 (MQ=255)
           tatCGTATGGTTTTTCATATTCATACATATTTATTAGGGAATTATGGCTGATTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATACTCTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaa                                                                                                                                   <  2:148736/150‑1 (MQ=255)
            atCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                  >  1:1873067/1‑150 (MQ=255)
             tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAg                                                                                                                                 <  1:1701543/150‑1 (MQ=255)
              cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGATTGTTTCAAAGt                                                                                                                                >  2:1352395/1‑150 (MQ=255)
              cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctct                                                                                                                                <  2:489580/150‑6 (MQ=38)
               gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTATGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGTGATAGTTTAAGAGGGTAAGAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTa                                                                                                                               <  2:1506003/150‑1 (MQ=255)
                tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctctta                                                                                                                              <  1:1243265/150‑8 (MQ=38)
                 aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                             <  2:1559018/150‑1 (MQ=255)
                     tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                         <  1:169104/150‑1 (MQ=255)
                      ttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                        >  1:933755/1‑150 (MQ=255)
                       tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                       <  2:658457/150‑1 (MQ=255)
                         tcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGTTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAg                                                                                                                     <  2:1480363/150‑1 (MQ=255)
                          aatattcataCATTTTTATTAGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                   <  1:995921/149‑1 (MQ=255)
                            tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTTTTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTGACAAAGTAAGAATGGGAGttt                                                                                                                  >  2:1015709/1‑150 (MQ=255)
                               tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                               >  1:1951691/1‑150 (MQ=255)
                               tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                               <  1:687451/150‑1 (MQ=255)
                                cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                              >  2:1373722/1‑150 (MQ=255)
                                 ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACGAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTg                                                                                                             >  1:1536759/1‑150 (MQ=255)
                                   aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                           <  2:173943/150‑1 (MQ=255)
                                         ttattaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTTAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCg                                                                                                     <  1:45383/150‑1 (MQ=255)
                                           attaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGACGGTGGGTAAAGAAACGTCTTGTTACAAAGTAAGAATGTGAGTTTAACTGCcacatctaa                                                                                                   >  1:143953/1‑145 (MQ=255)
                                            ttaGTGATTTATGGCTGTTTAACTAAGTGTTGTTAATTTGACTTAAGTAAGAATGATTATTAGTGGGATAGTTTAAGAGGGAAACAAGCCGGTGGGTACAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACt                                                                                                  <  1:1274178/150‑1 (MQ=255)
                                             taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                                 >  1:342008/1‑150 (MQ=255)
                                             taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                                 >  1:79607/1‑150 (MQ=255)
                                               gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                               >  2:1210864/1‑150 (MQ=255)
                                               gggATTTATGGCTCTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAACGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                               >  2:1660318/1‑150 (MQ=255)
                                                ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTCAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                              <  2:1343125/150‑1 (MQ=255)
                                                ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                              <  1:1645776/150‑1 (MQ=255)
                                                  aTTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAg                                                                                            <  2:1873067/150‑1 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCTCCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                         >  2:424486/1‑150 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                         <  1:1747218/150‑1 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                         >  2:859693/1‑150 (MQ=255)
                                                       ttgatgtgTAACTAAGTTTGTTTATTTTGTCTTAATTAATCATGATTATTAGTTGGATAGTTTAAGATGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                       <  1:270184/146‑1 (MQ=255)
                                                       tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                       <  1:865300/150‑1 (MQ=255)
                                                            gTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTTGGATAGTTTAAGAGTGTAACAAGCCGGTGGGTAAAGCACCTGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGAcc                                                                                  <  2:1736993/150‑1 (MQ=255)
                                                               tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                               >  1:1582908/1‑150 (MQ=255)
                                                               tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                               <  1:1029682/150‑1 (MQ=255)
                                                               tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACAGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACAGCt                                                                               >  1:1684136/1‑150 (MQ=255)
                                                                aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                              >  1:1461173/1‑150 (MQ=255)
                                                                aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                              <  2:2099812/150‑1 (MQ=255)
                                                                 aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                             >  1:727241/1‑150 (MQ=255)
                                                                    aaGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttgttg                                                                          <  1:1660963/150‑1 (MQ=255)
                                                                    aaGTGTCGCTAATTTGACTTAAGTAAGCCTCCTTATTAGTGGGATAGTTTCAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTCAGAATGGGAGTTTAACTGCCCCAGCGACTTGGCAGATAGCGGACCGCttgttg                                                                          <  2:211003/150‑1 (MQ=255)
                                                                     aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGTCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                         >  1:158068/1‑150 (MQ=255)
                                                                      gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                        <  2:513504/150‑1 (MQ=255)
                                                                      gtgtGGGTTATTAGACTTAAGTAAGCATGATTATTAGTGGCAAAGTATAATAGGGAAACAAGCCGGTGGGTAAAGCACGGGCTTGTGACAAAGTAAGAATGGGAGTGTAACTGCCCCAGGGACTTAGCAGATAGCATACCGCgtggggag                                                                        >  2:84394/1‑145 (MQ=255)
                                                                        gtgGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGta                                                                      >  1:217135/1‑149 (MQ=255)
                                                                          ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                    >  1:1493420/1‑150 (MQ=255)
                                                                          ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                    >  1:1500541/1‑150 (MQ=255)
                                                                            ttAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTAGTTGAGTCTGCg                                                                  >  1:929194/1‑150 (MQ=255)
                                                                             tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                 <  2:1656313/150‑1 (MQ=255)
                                                                               aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCAGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                               <  1:1325100/150‑1 (MQ=255)
                                                                                 ttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTa                                                             <  2:477447/150‑1 (MQ=255)
                                                                                     cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTTCAAAGTAAGAATGGGAGTTTAACTGACCCAGCGACTTTGCAGATAGCTGACAGCTTGTTGAGTCTGCGGTTTTTTCa                                                         >  1:1256959/1‑150 (MQ=255)
                                                                                     cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCa                                                         >  1:1508678/1‑150 (MQ=255)
                                                                                        aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                      <  2:160567/150‑1 (MQ=255)
                                                                                        aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                      >  1:1332523/1‑150 (MQ=255)
                                                                                           taagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGt                                                   <  1:781624/150‑1 (MQ=255)
                                                                                              gCATGATTCTTAGGGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGACTGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGGCTGCGGTTTATTCAGATAGTCCt                                                <  2:302251/150‑1 (MQ=255)
                                                                                              gCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTAGAGTCTGCGGTTTATTCAGATAGTCCt                                                >  2:1521957/1‑150 (MQ=255)
                                                                                               cATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTc                                               >  2:770332/1‑150 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTTTGTAAAGCACCTGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                            <  1:1143126/150‑1 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                            >  2:1396058/1‑150 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCACACg                                            >  2:1248894/1‑150 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCAAGCGACTTTGCAGATAGCTGACCGCTTGTTTAGTCTGCGGTATATTCAGATAGTCATCACg                                            >  1:753318/1‑150 (MQ=255)
                                                                                                   attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACgg                                           >  1:953679/1‑150 (MQ=255)
                                                                                                      attaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                        <  2:732073/150‑1 (MQ=255)
                                                                                                          gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGa                                    <  1:1181422/150‑1 (MQ=255)
                                                                                                           tGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGTGAGTTTAACTGCCCCAGCGACTTAGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTAATTCAGATAGTCCTCACGGAACAAGAt                                   >  2:2051600/1‑150 (MQ=255)
                                                                                                           tGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGAt                                   >  1:883939/1‑150 (MQ=255)
                                                                                                            gggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                  >  1:34175/1‑150 (MQ=255)
                                                                                                             ggATAGTATAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGCGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTTTTGAGTCTGCGGTTAATTCAAATAGTCCTCACGTAACAAGATgg                                 >  1:1654550/1‑150 (MQ=255)
                                                                                                                tAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGc                              <  2:1948185/150‑1 (MQ=255)
                                                                                                                 aGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGcc                             >  1:2028584/1‑150 (MQ=255)
                                                                                                                  gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                            >  1:1597534/1‑150 (MQ=255)
                                                                                                                  gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTAGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                            >  1:580131/1‑150 (MQ=255)
                                                                                                                     tAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTa                         >  2:1409242/1‑150 (MQ=255)
                                                                                                                     aaaGACGGTAACAACCCGCTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGGTCCACTGCCCCAGCGACTTTGCAGATAGCTGCCCGCTCGTCGACGCTGCGATTTATTCAGATAGTCCTCACCGACCAAGATCGGGCCGCTa                         <  2:1484743/149‑1 (MQ=255)
                                                                                                                       agagGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAAt                       >  2:1140137/1‑150 (MQ=255)
                                                                                                                        gagGGTAACAAGCCGGTGGGTAAAGCAGCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGATGGTGCCGCTAAtt                      >  1:141564/1‑150 (MQ=255)
                                                                                                                          gggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTGACGGAACAAGATGGTGCGGCTAATTTc                    >  1:1346059/1‑150 (MQ=255)
                                                                                                                          gggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACCGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTc                    >  1:1040872/1‑150 (MQ=255)
                                                                                                                           ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                   >  2:946902/1‑150 (MQ=255)
                                                                                                                            gTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                  <  1:1375367/150‑1 (MQ=255)
                                                                                                                             tAACAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCggg                 >  2:1393529/1‑150 (MQ=255)
                                                                                                                                cAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTATGTATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCTCGGAACAAGATGGTGCCGCTAATTTCGGGcac              <  1:126791/150‑1 (MQ=255)
                                                                                                                                cAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac              >  2:651475/1‑150 (MQ=255)
                                                                                                                                cAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCATATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac              >  1:854500/1‑150 (MQ=255)
                                                                                                                                cAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac              >  1:2080766/1‑150 (MQ=255)
                                                                                                                                cAAGCCGGGGCGCAAAGCACCGGCTTGTTACAAAGTAACAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGGTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac              <  2:1527280/150‑1 (MQ=255)
                                                                                                                                 aaGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCTGATAGTCCTCACGGAACACGATGGTGCCGCTAATTTCGGGcaca             >  2:1012094/1‑150 (MQ=255)
                                                                                                                                  aGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg            <  1:532338/150‑1 (MQ=255)
                                                                                                                                      ggTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATc        >  2:1490584/1‑150 (MQ=255)
                                                                                                                                          ggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTa    <  2:1396295/150‑1 (MQ=255)
                                                                                                                                            tAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAg  <  2:342008/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAG  >  NC_000913/1567328‑1567617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: