Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,567,472 G→C 5.5% intergenic (‑332/+32) dosC ← / ← yddW diguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,567,4720GC 5.5% 393.4 / 12.5 128intergenic (‑332/+32)dosC/yddWdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator/liprotein, glycosyl hydrolase homolog
Reads supporting (aligned to +/- strand):  ref base G (63/58);  new base C (4/3);  total (67/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.20e-01

Only 100 of 130 total aligned reads displayed.

GTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCG  >  NC_000913/1567328‑1567621
                                                                                                                                                |                                                                                                                                                     
      tcGAATATCGTATGGGTTTTCATATTCATAAATTTTTATTAGTGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                            <  1:610937/149‑1 (MQ=255)
      gctaaTATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCGGGTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGt                                                                                                                                            <  2:1067794/147‑1 (MQ=255)
       ctaaTAACTTATTGCTTTTCATAATCCAACATGAATCTTAGTGAGATATTGCTGTTTACCTAACTCTCGTTAATTTGACTTAATTAACCATGATTATTAGTTGGATAGTTTACGCGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGtt                                                                                                                                           <  2:353516/148‑1 (MQ=255)
        gAATATCGAATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTa                                                                                                                                          <  1:1995893/150‑1 (MQ=255)
            atCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACaaa                                                                                                                                      >  1:1873067/1‑150 (MQ=255)
             tCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCAGGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAg                                                                                                                                     <  1:1275242/150‑1 (MQ=255)
              cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGATTGTTTCAAAGt                                                                                                                                    >  2:1352395/1‑150 (MQ=255)
              cGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctct                                                                                                                                    <  2:489580/150‑6 (MQ=38)
               gTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTATGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGTGATAGTTTAAGAGGGTAAGAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTa                                                                                                                                   <  2:1506003/150‑1 (MQ=255)
                tATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGCTTTACCCACCGGCTTGTTAccctctta                                                                                                                                  <  1:1243265/150‑8 (MQ=38)
                 aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                                 <  1:894458/150‑1 (MQ=255)
                 aTGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAg                                                                                                                                 <  2:1559018/150‑1 (MQ=255)
                    gTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAAt                                                                                                                              >  2:1181422/1‑150 (MQ=255)
                     tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                             <  1:169104/150‑1 (MQ=255)
                     tttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATg                                                                                                                             <  1:1483171/150‑1 (MQ=255)
                      ttttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATgg                                                                                                                            >  1:933755/1‑150 (MQ=255)
                       tttCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATggg                                                                                                                           <  2:658457/150‑1 (MQ=255)
                        ttcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGa                                                                                                                          <  2:1668506/150‑1 (MQ=255)
                         tcatattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGTTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAg                                                                                                                         <  2:1480363/150‑1 (MQ=255)
                          aatattcataCATTTTTATTAGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGtt                                                                                                                       <  1:995921/149‑1 (MQ=255)
                            tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTTTTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTGACAAAGTAAGAATGGGAGttt                                                                                                                      >  2:1015709/1‑150 (MQ=255)
                            tattcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGttt                                                                                                                      <  1:1367094/150‑1 (MQ=255)
                             attcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCATGTTACAAAGTAAGAATGGGAGTTTa                                                                                                                     >  2:1998824/1‑150 (MQ=255)
                               tcataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAAc                                                                                                                   <  1:687451/150‑1 (MQ=255)
                                cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                                  >  2:1373722/1‑150 (MQ=255)
                                cataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACt                                                                                                                  <  1:1663189/150‑1 (MQ=255)
                                 ataCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACGAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTg                                                                                                                 >  1:1536759/1‑150 (MQ=255)
                                   aCATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcc                                                                                                               <  2:173943/150‑1 (MQ=255)
                                    cATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGccc                                                                                                              >  1:1636295/1‑150 (MQ=255)
                                     aTTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGAGTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGcccc                                                                                                             >  2:1005162/1‑150 (MQ=255)
                                      tttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACAGGCTTGTTACATATTAAGAATGGGAGTTTAACTGcccaa                                                                                                            >  1:286860/1‑148 (MQ=255)
                                       ttttATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAg                                                                                                           <  1:799484/150‑1 (MQ=255)
                                         ttattaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTTAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCg                                                                                                         <  1:45383/150‑1 (MQ=255)
                                           attaGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGACGGTGGGTAAAGAAACGTCTTGTTACAAAGTAAGAATGTGAGTTTAACTGCcacatctaa                                                                                                       >  1:143953/1‑145 (MQ=255)
                                             taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                                     >  2:1549530/1‑150 (MQ=255)
                                             taGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACtt                                                                                                     >  1:79607/1‑150 (MQ=255)
                                               gggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                                   >  2:1210864/1‑150 (MQ=255)
                                               gggATTTATGGCTCTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCAACGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTg                                                                                                   >  2:1660318/1‑150 (MQ=255)
                                                ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTCAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                  <  2:1343125/150‑1 (MQ=255)
                                                ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                  <  1:543724/150‑1 (MQ=255)
                                                ggATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGc                                                                                                  <  1:1645776/150‑1 (MQ=255)
                                                 gATTTCTGGCTCTCTAACTAAGTGTGGCTAATTTGACTCAACTAAGCATGATTATTAGTCGGACAGGCTCCGAGGGTAACACGCCGGGGGGGAAAGCACCGGCTTGTTACAAAGTAAGAATGCGCGTTTAACTGCCCCAGCGACTTTGCa                                                                                                 <  2:1448122/150‑1 (MQ=255)
                                                  aTTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAg                                                                                                <  2:1873067/150‑1 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCTCCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                             >  2:424486/1‑150 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                             <  1:1747218/150‑1 (MQ=255)
                                                     tATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATa                                                                                             >  2:859693/1‑150 (MQ=255)
                                                       ttgatgtgTAACTAAGTTTGTTTATTTTGTCTTAATTAATCATGATTATTAGTTGGATAGTTTAAGATGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                           <  1:270184/146‑1 (MQ=255)
                                                       tGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGc                                                                                           <  1:865300/150‑1 (MQ=255)
                                                        ggCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGAAGATAgat                                                                                          >  2:1329429/1‑148 (MQ=255)
                                                        ggCTGTTTAACTAAGTGTGGATAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCt                                                                                          >  2:268895/1‑150 (MQ=255)
                                                               tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCt                                                                                   <  1:1029682/150‑1 (MQ=255)
                                                               tAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACAGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACAGCt                                                                                   >  1:1684136/1‑150 (MQ=255)
                                                                aaCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCtt                                                                                  <  2:2099812/150‑1 (MQ=255)
                                                                 aCTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCttg                                                                                 >  1:727241/1‑150 (MQ=255)
                                                                    aaGTGTGGTCAATATGACTTAATTAAGCCTGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGCTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAACCGGAGTTTAACTGCCCCAGCGACGTTGCAGATACCTGACCGCttgtag                                                                              <  2:1497769/150‑3 (MQ=255)
                                                                    aaGTGTCGCTAATTTGACTTAAGTAAGCCTCCTTATTAGTGGGATAGTTTCAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTCAGAATGGGAGTTTAACTGCCCCAGCGACTTGGCAGATAGCGGACCGCttgttg                                                                              <  2:211003/150‑1 (MQ=255)
                                                                     aGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGTCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGa                                                                             >  1:158068/1‑150 (MQ=255)
                                                                      gtgtGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAg                                                                            <  2:513504/150‑1 (MQ=255)
                                                                      gtgtGGGTTATTAGACTTAAGTAAGCATGATTATTAGTGGCAAAGTATAATAGGGAAACAAGCCGGTGGGTAAAGCACGGGCTTGTGACAAAGTAAGAATGGGAGTGTAACTGCCCCAGGGACTTAGCAGATAGCATACCGCgtggggag                                                                            >  2:84394/1‑145 (MQ=255)
                                                                          ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                        >  1:719164/1‑150 (MQ=255)
                                                                          ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                        >  1:1493420/1‑150 (MQ=255)
                                                                          ggTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTg                                                                        >  1:1500541/1‑150 (MQ=255)
                                                                             tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                     <  2:1656313/150‑1 (MQ=255)
                                                                             tAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCgg                                                                     <  1:1856497/150‑1 (MQ=255)
                                                                               aTTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCAGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGtt                                                                   <  1:1325100/150‑1 (MQ=255)
                                                                                 ttGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTa                                                                 <  2:477447/150‑1 (MQ=255)
                                                                                     cTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTTCAAAGTAAGAATGGGAGTTTAACTGACCCAGCGACTTTGCAGATAGCTGACAGCTTGTTGAGTCTGCGGTTTTTTCa                                                             >  1:1256959/1‑150 (MQ=255)
                                                                                      ttAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAg                                                            >  1:2067534/1‑150 (MQ=255)
                                                                                        aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                          >  1:1332523/1‑150 (MQ=255)
                                                                                        aagtaagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGAt                                                          <  2:160567/150‑1 (MQ=255)
                                                                                           taagCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGt                                                       <  1:781624/150‑1 (MQ=255)
                                                                                              gCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTAGAGTCTGCGGTTTATTCAGATAGTCCt                                                    >  2:1521957/1‑150 (MQ=255)
                                                                                               cATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTc                                                   >  2:770332/1‑150 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTTTGTAAAGCACCTGATTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                                <  1:1143126/150‑1 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACg                                                >  2:1396058/1‑150 (MQ=255)
                                                                                                  gATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCACACg                                                >  2:1248894/1‑150 (MQ=255)
                                                                                                   attattAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACgg                                               >  1:953679/1‑150 (MQ=255)
                                                                                                    ttattaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGa                                              >  2:598390/1‑150 (MQ=255)
                                                                                                      attaGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAAc                                            <  2:732073/150‑1 (MQ=255)
                                                                                                          gTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGa                                        <  1:1181422/150‑1 (MQ=255)
                                                                                                           tGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGAt                                       >  1:883939/1‑150 (MQ=255)
                                                                                                            gggATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATg                                      >  1:34175/1‑150 (MQ=255)
                                                                                                             ggATAGTTAAAGCGGGAAACAAGCCGGGGGGGAACGCACCGGCTTGTTACAAAGTAAGCATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTGGTGGAGTCTGCGGTTTATTCAGATACTCCTCACGGAACAAGATgg                                     <  2:1394940/150‑1 (MQ=255)
                                                                                                             ggATAGTATAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGCGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTTTTGAGTCTGCGGTTAATTCAAATAGTCCTCACGTAACAAGATgg                                     >  1:1654550/1‑150 (MQ=255)
                                                                                                                tAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGc                                  <  2:1948185/150‑1 (MQ=255)
                                                                                                                 aGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGcc                                 >  1:2028584/1‑150 (MQ=255)
                                                                                                                  gTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCg                                >  1:1597534/1‑150 (MQ=255)
                                                                                                                    ttAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTATGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCt                              >  1:1516149/1‑150 (MQ=255)
                                                                                                                     aaaGACGGTAACAACCCGCTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGGTCCACTGCCCCAGCGACTTTGCAGATAGCTGCCCGCTCGTCGACGCTGCGATTTATTCAGATAGTCCTCACCGACCAAGATCGGGCCGCTa                             <  2:1484743/149‑1 (MQ=255)
                                                                                                                        gagGGTAACAAGCCGGTGGGTAAAGCAGCGGCTTGTTACAAAGTAAGAATGGGAGTTTATCTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGATCAAGATGGTGCCGCTAAtt                          >  1:141564/1‑150 (MQ=255)
                                                                                                                          gggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACCGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTc                        >  1:1040872/1‑150 (MQ=255)
                                                                                                                           ggTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCg                       >  2:946902/1‑150 (MQ=255)
                                                                                                                            gTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCgg                      <  1:1375367/150‑1 (MQ=255)
                                                                                                                                cAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTATGTATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCTCGGAACAAGATGGTGCCGCTAATTTCGGGcac                  <  1:126791/150‑1 (MQ=255)
                                                                                                                                cAAGCCGGTGCTTTACCCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGcac                  >  1:2080766/1‑150 (MQ=255)
                                                                                                                                  aGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAg                <  1:532338/150‑1 (MQ=255)
                                                                                                                                      ggTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATc            >  2:1490584/1‑150 (MQ=255)
                                                                                                                                          ggTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTa        <  2:1396295/150‑1 (MQ=255)
                                                                                                                                            tAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAg      <  2:342008/150‑1 (MQ=255)
                                                                                                                                                gCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATcc  >  2:1075153/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAAGCATGATTATTAGTGGGATAGTTTAAGAGGGTAACAAGCCGGTGGGTAAAGCACCGGCTTGTTACAAAGTAAGAATGGGAGTTTAACTGCCCCAGCGACTTTGCAGATAGCTGACCGCTTGTTGAGTCTGCGGTTTATTCAGATAGTCCTCACGGAACAAGATGGTGCCGCTAATTTCGGGCACAGCATCGTTAAGATCG  >  NC_000913/1567328‑1567621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: