Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 5.4% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 5.4% 402.9 / 7.5 142intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (66/67);  new base T (4/5);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 144 total aligned reads displayed.

GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT  >  NC_000913/1864614‑1864911
                                                                                                                                                     |                                                                                                                                                    
 ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                     <  1:804248/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACTTTTGTTTGTGAAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAAGCTTCACATCTGGCGCAATCCATTCGCGTTGCGAAACGGTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                    <  1:767275/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAATGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATg                                                                                                                                                    <  2:1834991/150‑1 (MQ=255)
  cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAtt                                                                                                                                                    <  1:942169/150‑2 (MQ=255)
  cGGATGATGGCTACAAAACATTTGATTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGCTGTGTGCCGGGTGCAATg                                                                                                                                                    <  2:388241/150‑1 (MQ=255)
       tattgCTACAAAACATTTTTTTGGGTATAAACGGCTTACGCTTCAGAAACGCAAACATTGAGCAACGAGAAACCTGCACATCTGGCGCAATCCATTCGCGGTGGGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATTCATcc                                                                                                                                               <  2:340306/146‑1 (MQ=255)
        atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAACTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTAGTGTGCCGGGTGCAATGCATCCg                                                                                                                                              <  2:1313882/150‑1 (MQ=255)
          ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAATGTGACCAAAGAGAAACCTTCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGc                                                                                                                                            <  1:1987600/150‑1 (MQ=255)
          ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                            <  1:1183582/150‑1 (MQ=255)
           gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCATAAACGCAAAAATTGACCAAAGAGAAACCTGCACATCTGGCTCAATCCATTCGCTTTGCGAAACGTTAAATTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                           >  2:1223965/1‑150 (MQ=255)
             tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTTCGTTAATGTTGTGTGCCGGGTTCAATTCATCCTGcaca                                                                                                                                         >  1:1323120/1‑150 (MQ=255)
             tACAAAACAATTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                         <  1:1547024/150‑1 (MQ=255)
               cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                       >  2:19290/1‑150 (MQ=255)
                aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                      <  2:1559826/150‑1 (MQ=255)
                aaaaCATTTGTTTTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACacaac                                                                                                                                     >  2:909187/1‑150 (MQ=255)
                 aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                     >  2:1404222/1‑150 (MQ=255)
                     aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCTGGTGCAATGCATCCGGCACACAACATcc                                                                                                                                 >  2:106245/1‑149 (MQ=255)
                          tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                            <  2:594429/150‑1 (MQ=255)
                              tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCTCCTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCATCATCACACCATATc                                                                                                                        >  2:148696/1‑150 (MQ=255)
                               gtAGAAAGGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAGGTAAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                       <  2:1656890/150‑1 (MQ=255)
                               gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCCCACCATATcc                                                                                                                       >  2:1851095/1‑150 (MQ=255)
                               gtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                       <  2:1509150/150‑1 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                      >  2:2102989/1‑150 (MQ=255)
                                    aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCCCACCAAATCCAgcgc                                                                                                                  >  2:1582614/1‑150 (MQ=255)
                                        gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATATCCAGCGCAGtt                                                                                                              >  1:1424084/1‑150 (MQ=255)
                                           tACGCTTCAGAAACGCAAAAAGTGACCAATGAGAAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                           <  1:343626/150‑1 (MQ=255)
                                           tACGCTTCAGAAACGCAAAAAGTGACCAACGAGAACCCTGCACATCTGGCGCATACCATTCGCGTTGCGAAACGTTAATTTACGTAAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                           <  2:1944665/150‑1 (MQ=255)
                                           tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCAGCGCAGttttt                                                                                                           >  1:1841505/1‑150 (MQ=255)
                                            aCGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCCTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                          <  2:1386368/150‑1 (MQ=255)
                                            aCGCTTCGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                         <  2:96716/150‑1 (MQ=255)
                                              gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                        >  1:1197650/1‑150 (MQ=255)
                                               cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTTCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                       >  2:381353/1‑150 (MQ=255)
                                                  cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                    <  2:1175508/150‑1 (MQ=255)
                                                  cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                    >  2:1098213/1‑150 (MQ=255)
                                                  cAGAAACGCAAAAACTGACCCACGCGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGAATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                    <  2:863937/150‑1 (MQ=255)
                                                    gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGt                                                                                                  >  1:467797/1‑150 (MQ=255)
                                                    gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCAGGCACACAACCAAAACCCAAATCCAGCGCAGTTTTTAGTTTTGGt                                                                                                  >  1:941731/1‑150 (MQ=255)
                                                         gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCTGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                             <  2:1651265/150‑1 (MQ=255)
                                                         gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                             >  1:1200335/1‑150 (MQ=255)
                                                          cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCggt                                                                                            >  2:382445/1‑149 (MQ=255)
                                                           aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                           >  1:665586/1‑150 (MQ=255)
                                                            aacaGTTACCAAAGAGACACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTAATTAACTTTAATGTTGTGTGCCGGGTGCAATGCATCGGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGata                                                                                          <  1:602538/147‑1 (MQ=255)
                                                               tgggACCAAAGATAAACCTCCACATGTGGCGCAAACCATTCGCGTTGCGAAACCTAAATTTACTTTATTTTTGTGTGCGGGGTGCAATCCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                       <  1:1697999/147‑1 (MQ=255)
                                                                ttGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGcc                                                                                      <  2:2062897/149‑1 (MQ=255)
                                                                 tGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACATGACCCCAAATCCAGCGGAGTTTTTCCGTTTGGTGGCGGGTTTGCCt                                                                                     >  1:990195/1‑150 (MQ=255)
                                                                  gACCAAAGAGAAACCTGCACATCTGGCGCAATCCTTTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGGGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCtt                                                                                    <  1:916218/150‑1 (MQ=255)
                                                                    ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTAt                                                                                  >  2:161436/1‑150 (MQ=255)
                                                                       aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                               >  2:779041/1‑150 (MQ=255)
                                                                        agagAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAg                                                                              <  2:582381/150‑1 (MQ=255)
                                                                         tacaacccTGCACTTGTGGCGAAATCCTTTGGTGTTGCTAAAGTTTAATTTATGTTATTGTGGGGGGCGGGGGGCATGGCAGCCGGCAAACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                             <  2:1888062/144‑1 (MQ=255)
                                                                         gagaAACCTGCACATCTGTCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTTCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCTGATTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                             <  2:1438705/150‑1 (MQ=255)
                                                                         gagaAACCTGAACATCTGTCGAAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGTGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                             <  2:773070/150‑1 (MQ=255)
                                                                          agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                            <  1:1446649/150‑1 (MQ=255)
                                                                          agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                            <  1:622363/150‑1 (MQ=255)
                                                                           gaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCAt                                                                           >  2:798228/1‑150 (MQ=255)
                                                                             atccTGCACATCTGGCGCAATCCATTGGCGTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAg                                                                         <  2:1488168/148‑1 (MQ=255)
                                                                               ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                       >  1:1982966/1‑150 (MQ=255)
                                                                               ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTGGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                       <  1:203900/150‑1 (MQ=255)
                                                                                cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATAACCCCATATCCAGCGCAGTTTTTACTTTTGGTGCCGGATATGCCATATACAGCATAGCTa                                                                      >  1:788395/1‑150 (MQ=255)
                                                                                     cttcgggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc                                                                 <  2:1690888/145‑1 (MQ=255)
                                                                                      aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCGGATTTGCCTTATACAGCATAGCTAATTACg                                                                >  1:456781/1‑150 (MQ=255)
                                                                                      aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                                <  1:34371/150‑1 (MQ=255)
                                                                                        cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGGAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                              >  1:1817762/1‑150 (MQ=255)
                                                                                        cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGACTTATACAGCATAGCTAATTACGCt                                                              >  1:1020712/1‑150 (MQ=255)
                                                                                          ggCGCAATCCATTCGCGTTGCGAACCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACCGCACACAACCTCACCCCATATACCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCGTATCCAGCATAGCTAAATCCCCTga                                                            >  2:1852499/1‑150 (MQ=255)
                                                                                           gcgcAATCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                           <  1:766392/150‑1 (MQ=255)
                                                                                              aaaTCCATTCGCGTTGCTAAACTTAAATTAACTTAAATGTGGTGTGCCGGGTGAAATGAATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                        <  1:909187/149‑1 (MQ=255)
                                                                                                   cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACATAACACCCTAACCAGCGCAGTTTTTCCTTTTGGTTCCGGATATTCCTTATACAGCATAGATAATTACACTGAAAAAAGtta                                                   >  2:1868069/1‑149 (MQ=255)
                                                                                                   cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCCTCCGGCACACAACATCACCCCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCTTATCCCGCATAGCTAATTCCGCGGAAGCAAGTTc                                                   >  2:1478435/1‑150 (MQ=255)
                                                                                                    aTTCGCGTTGCTAAACGTTATTTTACTTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                  <  2:966788/150‑1 (MQ=255)
                                                                                                    aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                  <  2:642095/150‑1 (MQ=255)
                                                                                                    aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCg                                                  <  1:1841923/150‑1 (MQ=255)
                                                                                                       cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                               <  1:13416/150‑1 (MQ=255)
                                                                                                       cgcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGACGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATACAGCATAGCTAATTCCGCTGAAGAAAGTTCGAcc                                               >  2:1828879/1‑150 (MQ=255)
                                                                                                            tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                          <  1:2061328/150‑1 (MQ=255)
                                                                                                             gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                         <  2:1361940/150‑1 (MQ=255)
                                                                                                             gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                         <  1:1373445/150‑1 (MQ=255)
                                                                                                              cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                        <  2:1294094/150‑1 (MQ=255)
                                                                                                                 aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGc                                    <  1:1404222/150‑1 (MQ=255)
                                                                                                                   cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                   >  1:597854/1‑150 (MQ=255)
                                                                                                                    tttAATTTACGTTAATGTTGTGTGCGGGGTGAATTGCATCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                  <  2:1993506/149‑1 (MQ=255)
                                                                                                                        aTTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAttt                             <  2:340860/150‑1 (MQ=255)
                                                                                                                         ttaaCGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTTGCCAGAATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAATCAACGCCGCAtat                             >  2:1355125/4‑148 (MQ=255)
                                                                                                                               ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                       <  1:1483750/150‑1 (MQ=255)
                                                                                                                               ttAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTgg                       <  2:484034/150‑1 (MQ=255)
                                                                                                                                tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCTCACCCTATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGGCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACCGCCGCATTTTGTTGGa                      >  2:846017/1‑150 (MQ=255)
                                                                                                                                      tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCCTAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg                >  1:417668/1‑150 (MQ=255)
                                                                                                                                      tgtgtgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGCCCTCAAGCACAGCCGCATTTTGTTGGACATggg                >  1:820836/1‑150 (MQ=255)
                                                                                                                                        tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATTGCTAATTCCGCTGAAGAAAGTTAGACCTCAAGCACAGCCGAATTTTGTTGGACATGGGCa              >  2:1049592/1‑150 (MQ=255)
                                                                                                                                           gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  2:1322282/1‑150 (MQ=255)
                                                                                                                                           gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  1:968207/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  1:1554424/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCACCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  2:64110/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGCATTGCACCCAGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc           >  1:1725719/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg          >  2:750512/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg          <  1:1402369/150‑1 (MQ=255)
                                                                                                                                                  gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCg    >  2:1644814/1‑150 (MQ=255)
                                                                                                                                                  gCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGTTGCCGGATATGCCTTATCCAGCATACATATTTCCGCTGAAGAAAGTGCGACCTCAAGCCCAGCCGCATTTTGTTGTACATGGGCAATCGTGGccc    >  2:407530/1‑149 (MQ=255)
                                                                                                                                                   cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTGCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCAGCACAGCCGCATTTTGTTGGAGATGGGCAATCGTGGCCGc   >  2:1608258/1‑150 (MQ=255)
                                                                                                                                                    aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt  >  2:1775694/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCT  >  NC_000913/1864614‑1864911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: