Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,160,882 A→C 10.0% T833P (ACG→CCG)  mdtC → multidrug efflux system, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,160,8820AC10.0% 273.2 / 2.3 124T833P (ACG→CCG) mdtCmultidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base A (45/63);  new base C (12/4);  total (57/67)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

ATGGCAACCGGCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTC  >  NC_000913/2160734‑2161026
                                                                                                                                                    |                                                                                                                                                
ttGGCTACCGGCGAATGCGCGACTCTCGGTGAATCCTCAGGGATTAGCGGCGGCCGCGACCATTTCGTTTAACCAGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGGAATGACCCAGCTTGGTGTGCCTTCGAc                                                                                                                                                 <  1:327236/149‑1 (MQ=255)
 tGGCAACCGGCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAAACTGCCGCCCGCAAAAACGCACTCGGACGCCAGTGCGGCGAACGATCGCCCAATGCCCCAGCTTGGTTTGCCTTCGCCg                                                                                                                                                >  2:1337350/1‑150 (MQ=255)
   gCAACCGGCGAATGCCACACTATCGGTGAATCATCTGGGATTATCGGCGGACTCGACCATTACGTTTAACCTGCCGACCGGAAAATCGCTCTAGGACGCCAGTGCGGCGCTCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGt                                                                                                                                              >  1:666256/1‑150 (MQ=255)
    cAACCGGCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTg                                                                                                                                             >  1:255024/1‑150 (MQ=255)
     aaCCGGCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTgc                                                                                                                                            <  1:705347/150‑1 (MQ=255)
         ggCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCCTTTCGTATTACCTGCCGCGCGGAAAATCGCTCTCGGACGCCACTGCGGCGATCGATCGCGCAATGACCCCGCGTGGTGTGCCTTCGACGGTGCGCgg                                                                                                                                        >  1:143379/1‑150 (MQ=255)
         ggCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGCCGCCAGTGCGGCGATCGATCGCGCCATGACCCAGCTTGGTGTGCCTTCGACCGTGCGCgg                                                                                                                                        >  2:1516937/1‑150 (MQ=255)
         ggCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGCCCCAGCTTGGTGTGCCTTCGACGGTGCGCgg                                                                                                                                        >  1:480408/1‑150 (MQ=255)
         ggCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCgg                                                                                                                                        >  2:1285361/1‑150 (MQ=255)
         ggCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCgg                                                                                                                                        >  2:1421684/1‑150 (MQ=255)
             aaTGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGGCCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGCTCGCGCAATCACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGt                                                                                                                                    >  2:417649/1‑150 (MQ=255)
             aaTGCCCCACTATCGGGGACTCATCAGGGATTATCGGCGGCCGCGACCATTTCGTTAACCCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGAGCGCGCAATGACCCCGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGt                                                                                                                                    <  2:2042296/150‑1 (MQ=255)
                  cccACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGc                                                                                                                               >  2:189841/1‑150 (MQ=255)
                   ccACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGcc                                                                                                                              >  1:832467/1‑150 (MQ=255)
                    cACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCg                                                                                                                             <  2:665652/150‑1 (MQ=255)
                    cACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCg                                                                                                                             <  1:1938682/150‑1 (MQ=255)
                         gcggggtatcatcAGGGAGTATCGGCGGCCTCGACCATTTCGGGTCACCTGCCGACCGGAACATCGCTCGCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCGTCGACGGTGCGCGGCTGCTTTGCCGGCACg                                                                                                                        <  1:351746/143‑1 (MQ=255)
                           ggTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGgc                                                                                                                      >  2:1236993/1‑150 (MQ=255)
                              gAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGCCGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCa                                                                                                                   >  2:1276888/1‑150 (MQ=255)
                              gAATCATCAGGGATTATCGGCGCCCTCGACCATTTCGTATAACCTGCCGACCGGAAAATCTCTCTATGACGCCAGTGCGGCGAGAGATCGCGCAATGAGCCACATTGGTTTGCCTTCTCCGGTGCGCGGCTGTTTTGCAGGCACGGCGCa                                                                                                                   >  2:1465411/1‑150 (MQ=255)
                                atcatcAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACGCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAgg                                                                                                                 >  1:1999918/1‑150 (MQ=255)
                                 tcatcaGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGCCCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGgt                                                                                                                >  2:1842406/1‑150 (MQ=255)
                                 tcatcaGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAACTCGCTCTCGGACGCCAGTGCGGCGAGCGCTCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCTCTGgt                                                                                                                >  1:330339/1‑150 (MQ=255)
                                  catcaGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGgtg                                                                                                               >  2:2017492/1‑150 (MQ=255)
                                    tcaGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGTCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGtt                                                                                                             >  1:1800225/1‑150 (MQ=255)
                                      aGGGATTATCGGCGGCCTCGCCCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTcc                                                                                                           >  2:332726/1‑150 (MQ=255)
                                      aGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTcc                                                                                                           <  1:1271716/150‑1 (MQ=255)
                                      aGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTcc                                                                                                           <  1:1455147/150‑1 (MQ=255)
                                        ggATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAg                                                                                                         >  2:672661/1‑150 (MQ=255)
                                           ttATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGTCCGGAATATCGCTCTCGGACGCCAGTGCGGCGAGCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGgag                                                                                                      <  1:332880/150‑1 (MQ=255)
                                             aTCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAACTCTCTCTCGGACGCCAGTGCGGCGAGCGCTCGCGCAATGACCCCGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGAc                                                                                                    <  1:2016224/150‑1 (MQ=255)
                                             aTCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTGGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGAc                                                                                                    <  1:1268512/150‑1 (MQ=255)
                                                ggcggcCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGTGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGAt                                                                                                 >  2:974753/1‑150 (MQ=255)
                                                ggcggcCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGAt                                                                                                 >  2:933240/1‑150 (MQ=255)
                                                ggcggcCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCGCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGAt                                                                                                 <  2:763263/150‑1 (MQ=255)
                                                 gcggcCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATg                                                                                                <  1:1895169/150‑1 (MQ=255)
                                                        cgcccATTTCGTTTAACCTGCCGACCGGAAAATCGCGCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGc                                                                                         <  2:1936984/147‑1 (MQ=255)
                                                        cGACCATTTCGTTTCACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGGGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGc                                                                                         <  2:1693260/150‑1 (MQ=255)
                                                             aTTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTg                                                                                    >  1:1296182/1‑150 (MQ=255)
                                                                 cGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATcc                                                                                <  1:1357374/150‑1 (MQ=255)
                                                                  gTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCt                                                                               <  2:494283/150‑1 (MQ=255)
                                                                    ttAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGTTCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCGGa                                                                             >  2:966247/1‑150 (MQ=255)
                                                                    gtacccTGCCGCCCGGAAAATCGCTCTCGGACGCCAGGGCGGCGATCGATCGCGCAATGACCCAGCGTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGa                                                                             <  2:1504739/146‑1 (MQ=255)
                                                                     tAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCCCGGCGCAGGTGTTCCAGGAGACGATGCACTCGCAGGTGATCCTGAt                                                                            >  1:1277841/1‑150 (MQ=255)
                                                                      aaCCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCTCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGAtt                                                                           >  2:1225365/1‑150 (MQ=255)
                                                                       acatGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTTACGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTa                                                                          >  2:1770328/4‑150 (MQ=255)
                                                                       aCCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGAttt                                                                          >  1:975477/1‑149 (MQ=255)
                                                                          tGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcg                                                                       >  2:1627176/1‑150 (MQ=255)
                                                                           gCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgc                                                                      <  1:258070/150‑1 (MQ=255)
                                                                           gCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGGGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgc                                                                      >  2:206558/1‑150 (MQ=255)
                                                                            ccGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTGGGTGGGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgcc                                                                     <  2:196667/150‑1 (MQ=255)
                                                                             cGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGCTCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGGCGATGAACTCGCAGGGGATCCTGATTATcgccg                                                                    >  2:1151718/1‑150 (MQ=255)
                                                                              gACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCGTGATTATcggcgc                                                                   >  1:385459/1‑150 (MQ=255)
                                                                              gACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgccgc                                                                   >  1:1522929/1‑150 (MQ=255)
                                                                              gACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgccgc                                                                   <  1:1494208/150‑1 (MQ=255)
                                                                                ccGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGCTCGCGCAATGACCCAGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATcgccgcaa                                                                 >  1:1600833/1‑148 (MQ=255)
                                                                                  ggAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGCCGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATc                                                               >  1:1174445/1‑150 (MQ=255)
                                                                                  ggAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATc                                                               >  1:655441/1‑150 (MQ=255)
                                                                                   gAAACTCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTTGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCATGTGTTGCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCg                                                              <  1:114748/150‑1 (MQ=255)
                                                                                   gAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCg                                                              <  1:578503/150‑1 (MQ=255)
                                                                                    aaaaTCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGc                                                             <  2:1106827/150‑1 (MQ=255)
                                                                                        tCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACg                                                         <  1:1421684/150‑1 (MQ=255)
                                                                                           ctctCGGCCGCCAGTGCGGCGATCGATCGCGCAATGACCCCGCTTGGTGTGCCTTCGCCGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCCGGAGTCGCTGAACTCGCAGGTGATCCTGCTTATCGCCGCCCTCGCCACGgtg                                                      >  1:1260557/1‑150 (MQ=255)
                                                                                           ctctCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGgtg                                                      >  1:1677516/1‑150 (MQ=255)
                                                                                           ctctCGGACGCCAGTGCGGCGAACGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGgtg                                                      <  2:763833/150‑1 (MQ=255)
                                                                                             ctcGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGGGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGta                                                    <  1:923241/150‑1 (MQ=255)
                                                                                               cGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTTGTGTGCCTTCGACGGTGCGCGGCAGGTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGtata                                                  <  1:1306733/150‑1 (MQ=255)
                                                                                               cGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGtata                                                  >  2:1150537/1‑150 (MQ=255)
                                                                                               cGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCGTGGGGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGtata                                                  <  1:781899/150‑1 (MQ=255)
                                                                                               cGGACGACAGGGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGGGCCTTCGCCGGTGGGCGGCAGTTTTGCCGGCAGGGCGCAGGTGTTCCAGGAGACGATTAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGtata                                                  <  2:1057242/150‑1 (MQ=255)
                                                                                                ggACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTatat                                                 <  2:909469/150‑1 (MQ=255)
                                                                                                  ccGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCg                                               <  1:189841/149‑1 (MQ=255)
                                                                                                   cGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGt                                              >  1:1685972/1‑150 (MQ=255)
                                                                                                     ccAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGc                                            <  1:332726/150‑1 (MQ=255)
                                                                                                       cgTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGGGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTg                                          <  1:550330/149‑1 (MQ=255)
                                                                                                         ggcggcgATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTggg                                        <  2:7584/149‑1 (MQ=255)
                                                                                                           cggcgATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTa                                      <  1:633289/150‑1 (MQ=255)
                                                                                                           cggcgATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTa                                      >  1:1667107/1‑150 (MQ=255)
                                                                                                           cggcgATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGATATACACCGTggcgatgg                                      <  1:1561298/150‑8 (MQ=255)
                                                                                                             gcgATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATc                                    >  1:80952/1‑150 (MQ=255)
                                                                                                               gatcgatcGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCt                                  <  1:450503/150‑1 (MQ=255)
                                                                                                                  cgatcgCGCATTGACCCAGCGTGGTGTTCCATCGCCGGTGCGCGTCAGTGTTGCCGGCACGGCGCAGGTGGTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTa                               <  1:1061961/150‑1 (MQ=255)
                                                                                                                   gatcgCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTAc                              >  2:855380/1‑150 (MQ=255)
                                                                                                                    ttcgCGCAATGACCCATGTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGTGACGATGAACTCGGAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACg                             <  1:197985/149‑1 (MQ=255)
                                                                                                                      cgcgCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACgag                           <  2:1522929/150‑1 (MQ=255)
                                                                                                                       gcgcAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACgaga                          >  2:383782/1‑150 (MQ=255)
                                                                                                                          cAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGtt                       >  1:1122351/1‑150 (MQ=255)
                                                                                                                           aaTGACCCAGCTTGGTGCGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTa                      <  1:1344082/150‑1 (MQ=255)
                                                                                                                               aCCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGTCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTa                  <  1:1752019/150‑1 (MQ=255)
                                                                                                                                cccAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTAc                 <  2:1393847/150‑1 (MQ=255)
                                                                                                                                 ccAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACa                <  1:112650/150‑1 (MQ=255)
                                                                                                                                   aGCTTGGGGTGCCTTCGACGGGGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATc              <  1:398422/150‑1 (MQ=255)
                                                                                                                                        ggTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTg         <  2:629033/150‑1 (MQ=255)
                                                                                                                                        ggTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTg         <  2:2054140/150‑1 (MQ=255)
                                                                                                                                          tgtgCCTTCGACGGTGCGCGGCTGTTGTGCCGGCACGGCGCAGGTGTTCGAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGAc       <  2:143379/150‑1 (MQ=255)
                                                                                                                                             gCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAt    <  1:245167/150‑1 (MQ=255)
                                                                                                                                             gCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAt    >  2:536569/1‑150 (MQ=255)
                                                                                                                                             gCCTTCGACGGTGCGCGGAAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAt    <  2:1594871/150‑1 (MQ=255)
                                                                                                                                              ccTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGAtt   >  2:1725665/1‑150 (MQ=255)
                                                                                                                                               cTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATtc  >  1:577013/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATGGCAACCGGCGAATGCCCCACTATCGGTGAATCATCAGGGATTATCGGCGGCCTCGACCATTTCGTTTAACCTGCCGACCGGAAAATCGCTCTCGGACGCCAGTGCGGCGATCGATCGCGCAATGACCCAGCTTGGTGTGCCTTCGACGGTGCGCGGCAGTTTTGCCGGCACGGCGCAGGTGTTCCAGGAGACGATGAACTCGCAGGTGATCCTGATTATCGCCGCCATCGCCACGGTGTATATCGTGCTGGGTATCCTTTACGAGAGTTACGTACATCCGCTGACGATTC  >  NC_000913/2160734‑2161026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: