Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,357,665 G→A 9.4% G254G (GGG→GGA yfaD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,357,6650GA 9.4% 306.6 / 14.3 120G254G (GGG→GGAyfaDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base G (38/68);  new base A (2/9);  total (43/77)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATT  >  NC_000913/2357516‑2357814
                                                                                                                                                     |                                                                                                                                                     
aaTGACAGCCAGCTACAAACCCTGTTGAATTATCTGCTGCACTTCGGAGATACCTCCCGTTTCACCCGGGTTATTGAGGAGATGGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGATGAACGGCTACGGCAGGAAggg                                                                                                                                                       >  1:1232794/1‑150 (MQ=38)
aaTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGATGAACGGCTACGGCAGGAAggg                                                                                                                                                       >  1:1437899/1‑150 (MQ=255)
 aTGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGc                                                                                                                                                      <  2:587755/150‑1 (MQ=255)
  tGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCa                                                                                                                                                     <  1:1756514/150‑1 (MQ=255)
   gACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGTAGATTGAAGAAAGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGTCAt                                                                                                                                                    >  2:1049715/1‑150 (MQ=38)
       gccagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGTGCATCaaa                                                                                                                                                >  1:712807/1‑150 (MQ=255)
         cagcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGGCATCATAtt                                                                                                                                              >  1:896693/1‑150 (MQ=255)
          agcTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtg                                                                                                                                             >  1:1647609/1‑150 (MQ=255)
           acTAGAAACACTGATTAATTATCTGCTGCATTCCGGCGATACCTCCCGTGTCACCAGTTTTATTGAGGAGTCTGACTAACGTTCACCTCTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATtgg                                                                                                                                            <  2:1751059/149‑1 (MQ=21)
            cTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggc                                                                                                                                           <  2:1344409/150‑1 (MQ=255)
             tACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTCCGGCAGGAAGGGCATCAAATtggct                                                                                                                                          >  1:1846843/1‑150 (MQ=255)
             tACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggct                                                                                                                                          <  2:1195143/150‑1 (MQ=255)
              aCAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctg                                                                                                                                         >  1:1239308/1‑150 (MQ=255)
                aaaCAATGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATtggctggc                                                                                                                                       <  2:973757/150‑1 (MQ=255)
                 aaCTCTGTTTAATTATCTGCTGCTATGCGGCGATACCTCCCGTTTGACCCGTTTTATTGATGAGATTGACTAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCa                                                                                                                                      <  2:955166/150‑1 (MQ=25)
                  acacTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAg                                                                                                                                     <  1:1501505/150‑1 (MQ=255)
                   cacTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTCTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAgg                                                                                                                                    <  1:746316/150‑1 (MQ=255)
                     cTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCAGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGaa                                                                                                                                  >  1:399690/1‑150 (MQ=255)
                       ttttAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAgg                                                                                                                                <  1:1434562/149‑1 (MQ=255)
                       gTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAgg                                                                                                                                >  1:960532/1‑150 (MQ=255)
                          tAATTATCAGCTGCAATGCGGCGATACCTCCCGGTGCCACCGTTATATTGAGGATATTTCCGAACTTTCACCCCTACAACAGAAGAGATAAATGACTATTGCTGACCGGCTACGGCAGGAAGGGAATCAAAGTGGCTGGCGGGaacgaat                                                                                                                             >  1:113678/1‑145 (MQ=255)
                           aaTTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATg                                                                                                                            <  1:559781/150‑1 (MQ=255)
                            aTTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc                                                                                                                           <  1:691973/150‑1 (MQ=255)
                            aTTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGCCATCAAATTGGCTGGCAGGAAGTTaaaa                                                                                                                           >  2:149739/1‑147 (MQ=255)
                             ttATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCa                                                                                                                          <  1:184150/150‑1 (MQ=255)
                             ttATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTCATCTTGAGGAGAATGCCTTACGGTCACCACTTCAAAAGGATAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATAGGCTGGCAGGAAGGTaaatt                                                                                                                          <  2:1472200/150‑5 (MQ=18)
                             ttATCTGCGGCAATGAGGCGAGACCTCCCGTTTCACCCGTTTTATTGATGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatt                                                                                                                          <  1:149739/150‑5 (MQ=21)
                              tATCTGCTGCAATTCGGCGATACCTCCCGTTTCACCCGTTTTATTTATGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatta                                                                                                                         <  2:1440161/150‑6 (MQ=21)
                              tATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt                                                                                                                         >  1:278926/1‑150 (MQ=255)
                              tATCCGCTGCAATGCGGCGATACCTCCCGATTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCAACACTACAAAAGGAGAGATAAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt                                                                                                                         <  1:1290502/150‑1 (MQ=255)
                                 ctgctgCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGGCTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGaa                                                                                                                      >  1:924386/1‑150 (MQ=255)
                                   gctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACa                                                                                                                    <  1:1607375/150‑1 (MQ=255)
                                    ctgcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAAcgt                                                                                                                   >  1:255862/1‑148 (MQ=255)
                                      gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGc                                                                                                                 >  1:13110/1‑150 (MQ=255)
                                      gcAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGc                                                                                                                 <  1:597222/150‑1 (MQ=255)
                                       cAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGcc                                                                                                                <  2:1687051/150‑1 (MQ=255)
                                        aaTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatta                                                                                                                         <  1:1139160/140‑6 (MQ=255)
                                        aaTGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTaaatta                                                                                                                         >  2:1139160/1‑135 (MQ=255)
                                            cggcgATACCTCCCTTTTCAACCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGTGATTAATGACTATTGCTGAATTGCTACGGCAGGACGGGAATCAAATTGGCTGGCAGGTAGGTATGCATGAACAAGCGATTat                                                                                                           <  2:1670299/150‑2 (MQ=255)
                                             ggcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTaaa                                                                                                          <  1:1208415/150‑1 (MQ=255)
                                              gcgATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAAGAACAAGACATTaaaa                                                                                                         >  1:1368958/1‑150 (MQ=255)
                                                  aaCCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTTCTGAACGGCTACTGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGc                                                                                                     <  1:243037/149‑1 (MQ=255)
                                                   aCCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCt                                                                                                    <  1:1529813/150‑1 (MQ=255)
                                                      acccGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGAATAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTg                                                                                                 <  1:1942295/149‑1 (MQ=255)
                                                       cccGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTgc                                                                                                <  1:1945708/150‑1 (MQ=255)
                                                        ccGTTACACCCGATTAATTGAGGAGATGGCCGAACGTTCACCACTCCAAAAGCAGAGAGTAATGACTATTGCTGAACGGCGACGGCAGGAAGGGCAGCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTgcg                                                                                               <  1:1021680/150‑1 (MQ=37)
                                                         cGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAATGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTgcgc                                                                                              <  2:1448941/150‑1 (MQ=255)
                                                              cACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCt                                                                                         <  1:726557/150‑1 (MQ=255)
                                                                cccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTgg                                                                                       <  2:136316/150‑1 (MQ=255)
                                                                cccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGAATGAACATTACATTTAAATTGCTTTTAGCATTCTgg                                                                                       >  1:536289/1‑150 (MQ=25)
                                                                 ccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAgg                                                                                                                                >  1:392430/1‑108 (MQ=255)
                                                                 ccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGa                                                                                      <  2:445641/150‑1 (MQ=255)
                                                                 ccGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAATTGCTTTGCGCATTCTGGa                                                                                      >  1:1701466/1‑150 (MQ=255)
                                                                 acGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAgg                                                                                                                                <  2:392430/107‑1 (MQ=255)
                                                                   gTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAATTGCTTTGAGCATGCTGGatc                                                                                    >  1:160486/1‑148 (MQ=255)
                                                                      ttATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAgg                                                                                 <  1:636737/150‑1 (MQ=255)
                                                                        aTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGc                                                                               <  2:2074914/150‑1 (MQ=255)
                                                                        aTTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGc                                                                               <  2:2066810/150‑1 (MQ=255)
                                                                         ttGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTTCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGt                                                                                                                               <  2:181719/101‑1 (MQ=255)
                                                                         ttGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGt                                                                                                                               >  1:181719/1‑101 (MQ=255)
                                                                          tGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCtt                                                                             >  1:37562/1‑150 (MQ=255)
                                                                          tGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCtt                                                                             <  1:1471769/150‑1 (MQ=255)
                                                                          tGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGACATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACCAGCCATTAAAATTGCTTTGCACATTCAGGAGCACGGCtt                                                                             >  1:167529/1‑150 (MQ=37)
                                                                            aggagATAGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACCGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTg                                                                           <  1:371008/150‑1 (MQ=255)
                                                                              gagaTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGaa                                                                         >  2:910853/1‑150 (MQ=255)
                                                                               agaTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAAATGCTTTGCGCATGCGGGAGCAGGACTTTGAAc                                                                        >  1:468829/1‑150 (MQ=255)
                                                                                gaTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACg                                                                       <  1:2089061/150‑1 (MQ=255)
                                                                                 aTTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATTAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGt                                                                      >  1:2091217/1‑150 (MQ=255)
                                                                                    gCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAATCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTgac                                                                   >  1:1122603/1‑149 (MQ=255)
                                                                                    gCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCGATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTgac                                                                   >  1:1583445/1‑149 (MQ=255)
                                                                                    gCCGAACGCTCACCACTACAAACGGAGAGATTAAAGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTgag                                                                   <  2:1062200/150‑1 (MQ=255)
                                                                                     ccGAACGTTCACCACTACAAAAGGAGAGATTAATGACAATTGCTGAACGGCTACGGCAGGAAGGCCATCAAATTGGCTGGCAGGAAGGTATGAATGAAACTGCCATTAAAATTGCTTTTCGCATGCTGGAGAAGAGCTTTGAACGTgaca                                                                  >  2:570603/1‑148 (MQ=25)
                                                                                       gAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGAtt                                                                <  1:219575/150‑1 (MQ=255)
                                                                                         aCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACATTCCATTTAAATTGCTTTGAGAATTCTTGAGCAGGTCTTTGAACATGACAAtgt                                                              >  2:322815/1‑150 (MQ=21)
                                                                                         aCGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATtgt                                                              <  2:278926/150‑1 (MQ=255)
                                                                                               accacTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGc                                                        <  1:480275/150‑1 (MQ=255)
                                                                                               accacTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGc                                                        <  1:1615061/150‑1 (MQ=255)
                                                                                                ccacTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAATAAGCCATTAAAATTGCTTTTATCATTCTGGAGCAGGGCTTTGAACGTGACATTGTGCTGGCg                                                       >  2:907608/1‑150 (MQ=25)
                                                                                                   cTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGAGCATGCTGGAGCAGGGCTTTGAACGTGACATTGTGCTGGCGAcc                                                    >  1:682988/1‑149 (MQ=255)
                                                                                                   atACAAAAGGAGAGATTAATGCCTACTGCTGCACGGCTACGGCAGGAAGGGCAGCAAATTGGATGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTATGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACa                                                    <  2:305744/149‑1 (MQ=37)
                                                                                                     aCAAACGGAGAGATTAATGTCTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTGGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAAc                                                  <  1:207856/150‑1 (MQ=255)
                                                                                                     aCAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGGATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAAc                                                  <  1:1810598/150‑1 (MQ=255)
                                                                                                       aaaaGGAGAGATTTATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGTAGGGCTTTGAACGTGAGATTGTGCTGGCGACAAccc                                                <  1:1527684/150‑1 (MQ=255)
                                                                                                           ggAGAGAGTAATGACTTTTGCTTAAAGTCTACTGCAGGAAGGGCATCAACTTGGCTGGCTGGACGGTAGGCATGAACAAGCCATTAAAATAGCTTTGCGCAGGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACt                                            <  2:113678/150‑1 (MQ=37)
                                                                                                              tagaTTAATGCCTATTGCGGAACGGCTACGGCAGTAAGGGCATCAAATTGGCGGGCAGGAAGGTATGCAGGAACCAGCCATTAAAATTGCTTTGCTCATGCTGTAGCAGGGCTTTGAACGTGAGATTGTGCTTGCGACAACCCAACTCAc                                         <  2:1232794/149‑1 (MQ=37)
                                                                                                                    aaTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGc                                   <  1:1734236/150‑1 (MQ=255)
                                                                                                                     aTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCTAAATGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCt                                  <  2:1998285/150‑1 (MQ=255)
                                                                                                                     aTGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAAATGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCt                                  <  1:1036108/150‑1 (MQ=255)
                                                                                                                       gACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGa                                <  1:520637/150‑1 (MQ=255)
                                                                                                                             tGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTcc                          <  1:973499/150‑1 (MQ=255)
                                                                                                                              gCTGAACGGCTACGGCATGAAGGGCATCAAATTGGCTGGCAGGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGGAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCg                         <  2:1609733/150‑1 (MQ=255)
                                                                                                                               cTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCTCATGCTGGAACAGGGCTTTGAACGTGACCATGTGCTGGCGCACACCCAGCTTACCGAAGCCGatatggccg                        >  2:48555/1‑145 (MQ=17)
                                                                                                                                tGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGGTATTCCGaa                       <  1:1057082/150‑1 (MQ=255)
                                                                                                                                 gAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAAc                      <  1:910853/150‑1 (MQ=255)
                                                                                                                                   aCGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTg                    <  2:37562/150‑1 (MQ=255)
                                                                                                                                             catgAAGGGAAACAAATTGGCTGGCAGGAAGGTATGCATGAACAAGACATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCTACAACCCAACTCACTGATGCTTATATTCCGAACTGTCATTAAAAg          <  1:1049715/147‑1 (MQ=37)
                                                                                                                                               ggAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGACATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCCACCTGTCAGTAAAAGga        >  1:699856/1‑150 (MQ=255)
                                                                                                                                                 aaGGGCATCAAATTGGCTGGCAGGAATGTATGCATGAACAAGCCATTAAAATTGCTTTGCTCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGgaga      <  1:1087490/150‑1 (MQ=255)
                                                                                                                                                 aaGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGgaga      <  1:1111649/150‑1 (MQ=255)
                                                                                                                                                     gCATCAATTTGGCTGGCAGGTAGGGATGCATGAACAAGCCATTAACATTGCTTTGCGCTTGCTGGAGCAGGGGTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGAtt  <  2:1936570/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATGACAGCCAGCTACAAACACTGTTTAATTATCTGCTGCAATGCGGCGATACCTCCCGTTTCACCCGTTTTATTGAGGAGATTGCCGAACGTTCACCACTACAAAAGGAGAGATTAATGACTATTGCTGAACGGCTACGGCAGGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAGCAGGGCTTTGAACGTGAGATTGTGCTGGCGACAACCCAACTCACTGATGCTGATATTCCGAACTGTCATTAAAAGGAGAGATT  >  NC_000913/2357516‑2357814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: