Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,405,389 G→A 6.4% intergenic (‑317/+314) nuoA ← / ← lrhA NADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,405,3890GA 6.4% 473.7 / 11.7 155intergenic (‑317/+314)nuoA/lrhANADH:ubiquinone oxidoreductase, membrane subunit A/transcriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base G (77/68);  new base A (6/4);  total (83/72)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

AACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTTG  >  NC_000913/2405240‑2405538
                                                                                                                                                     |                                                                                                                                                     
aaCAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTg                                                                                                                                                       >  2:940838/1‑150 (MQ=255)
 aCAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGa                                                                                                                                                      >  2:504914/1‑150 (MQ=255)
  cAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGAc                                                                                                                                                     >  2:210473/1‑150 (MQ=255)
   aaaaGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACa                                                                                                                                                    >  2:1343737/1‑150 (MQ=255)
   aaaaGTTTCTTCAAATTAGTTTACATAATATCACCACCATTAGCATTTAATGCCCTTTCACCCCATATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACa                                                                                                                                                    <  1:425392/150‑1 (MQ=255)
    aaaTTTTCTTAACATTAGTTAAAATAATATCAACACCATTACCATTTATTGCCCTTTCACCCCATATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAg                                                                                                                                                   <  1:863603/150‑1 (MQ=255)
         ttcttcACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAAtt                                                                                                                                              <  1:625464/150‑1 (MQ=255)
           cttcACATTAGTTTACATAATATCCACACAATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATATTAGATGTTGTTGAATCGTCTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTtgt                                                                                                                                           >  1:42347/1‑150 (MQ=255)
           cttcACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTAACAGGAAtttt                                                                                                                                            >  1:531044/1‑149 (MQ=255)
             tcACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTtgtg                                                                                                                                          >  2:44428/1‑150 (MQ=255)
                caTTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCg                                                                                                                                       >  1:1917506/1‑150 (MQ=255)
                       ttAAATAATATAAACACAATTAGCATTTAATGCCTTTTCCCCCCCTATCATTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATtcctc                                                                                                                                <  1:2024358/150‑1 (MQ=255)
                        tACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATtcctcc                                                                                                                               >  1:509576/1‑150 (MQ=255)
                            aaataTAAACACAATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTcccccc                                                                                                                           <  2:1720068/149‑1 (MQ=255)
                             aataTCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCa                                                                                                                          >  2:1813587/1‑150 (MQ=255)
                               tatCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGATGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCaaa                                                                                                                        >  2:216934/1‑150 (MQ=255)
                               aatCAACACCATTAGCATTTTATGCCCTTTCCCCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCaaa                                                                                                                        <  1:562046/149‑1 (MQ=255)
                                 tCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAag                                                                                                                      <  2:1505496/150‑1 (MQ=255)
                                    acacCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTAGTGTCGATTCCTCCCCCCAAAagaga                                                                                                                   >  2:762264/1‑150 (MQ=255)
                                     cacCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGATGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAagagag                                                                                                                  <  2:1098276/150‑1 (MQ=255)
                                       ccATTAGCATTTAATGCCCTTTCCCCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTAACAGGAATTTGTGTCGATTACGCCCCCCATAagagagga                                                                                                                >  1:1062368/1‑148 (MQ=255)
                                        cATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTAt                                                                                                               >  2:1542638/1‑150 (MQ=255)
                                           tAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTAtttt                                                                                                            <  1:928303/150‑1 (MQ=255)
                                             gCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCt                                                                                                          >  1:1730881/1‑150 (MQ=255)
                                               aTTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTg                                                                                                        <  1:1775678/150‑1 (MQ=255)
                                                   aaTGCCCATTAAACCCCTATCCTAGACGACTCCAGGATAATTAGATGTTGTTGAATCGTTTCCGTTGTTAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCt                                                                                                    <  2:462691/150‑1 (MQ=255)
                                                    aagCCCTTTCCCCCCAGCTCCTTGACGACTCCAGGAAAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtg                                                                                                   <  1:1123622/148‑1 (MQ=255)
                                                    aTGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtg                                                                                                   <  2:325220/150‑1 (MQ=255)
                                                    aTGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtg                                                                                                   >  1:432667/1‑150 (MQ=255)
                                                     tGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtgt                                                                                                  >  1:647953/1‑150 (MQ=255)
                                                      gCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTTTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtgtg                                                                                                 <  1:591319/150‑1 (MQ=255)
                                                      gCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtgtg                                                                                                 <  1:217101/150‑1 (MQ=255)
                                                      gCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTATTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCtgtg                                                                                                 <  1:1045893/150‑1 (MQ=255)
                                                        ccTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGac                                                                                               <  1:940838/150‑1 (MQ=255)
                                                         cTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGaca                                                                                              >  1:767031/1‑150 (MQ=255)
                                                              aCCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCtt                                                                                         >  1:1793371/1‑150 (MQ=255)
                                                               ccccAGATCCTTGACGCCTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTAGTTTCTTGATCTGTGACACGCatt                                                                                        >  2:1007954/1‑150 (MQ=255)
                                                               ccccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCttt                                                                                        >  1:1694644/1‑150 (MQ=255)
                                                               ccccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCACCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCttt                                                                                        >  1:2075673/1‑150 (MQ=255)
                                                                cccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCtttt                                                                                       >  1:543383/1‑150 (MQ=255)
                                                                cccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCttat                                                                                       >  1:266999/1‑148 (MQ=255)
                                                                 ccAGTTCATTGACGACTCCAGGATAATTAGATGTTGTTGTATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGTAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTg                                                                                      <  1:672979/150‑1 (MQ=255)
                                                                 ccAGATCCTTGACGACTCCAGGATTATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGTAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTg                                                                                      <  1:681960/150‑1 (MQ=255)
                                                                 ccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTg                                                                                      >  2:929464/1‑150 (MQ=255)
                                                                 ccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTg                                                                                      <  2:990289/150‑1 (MQ=255)
                                                                 ccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTg                                                                                      >  1:1641325/1‑150 (MQ=255)
                                                                 ccAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTATATTTTGACAGGATTTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGAACTGTGACACGCttttt                                                                                      >  2:1264930/1‑149 (MQ=255)
                                                                     aTCCTTTCCGACTCCAGGATAAATAGATGTAGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCAt                                                                                  <  2:305504/150‑1 (MQ=255)
                                                                       ccTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTc                                                                                >  1:744319/1‑150 (MQ=255)
                                                                       ccTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGGTGTGAAGCAATGGAAAAAATACGGGTCTATTTTAACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTAGTTTCTTGATCTGTGACACGCTTTTGTCATTc                                                                                >  2:267118/1‑150 (MQ=255)
                                                                        cTTTACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGTTTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTcc                                                                               <  1:1064377/150‑1 (MQ=255)
                                                                           gacgacTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTTAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCAATa                                                                            >  2:109585/1‑150 (MQ=255)
                                                                                  ccAGGATAATTAGTTGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACaaaaa                                                                     <  2:2039657/150‑1 (MQ=255)
                                                                                     ggATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGTGTCTATTTTAACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgc                                                                  <  2:1561669/150‑1 (MQ=255)
                                                                                     ggATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAATGAGAGTAATTTCTTCATCTGTGACACGCTTTTGTCATTCCATAACAAAAACga                                                                  >  1:1681734/1‑149 (MQ=255)
                                                                                      taTAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgca                                                                 <  1:1911438/149‑1 (MQ=255)
                                                                                      gATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgcc                                                                 >  2:178153/1‑149 (MQ=255)
                                                                                       aTAATTAGATGTTGTTGAATCTTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGGCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgcag                                                                <  2:1944651/150‑1 (MQ=255)
                                                                                       aTAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgcag                                                                <  1:1409143/150‑1 (MQ=255)
                                                                                        tAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACgcagc                                                               <  1:1422598/150‑1 (MQ=255)
                                                                                                  ttgttgAATCGTGTCGGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGTAATTTGTTTCGATTCCCCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTa                                                     <  1:1487369/150‑1 (MQ=255)
                                                                                                   tgttgAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCACCCCAAAAGAGAGTATTTTCTTGATATGTGAAACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTAc                                                    >  1:1852737/1‑150 (MQ=255)
                                                                                                   tgttgAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTAACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTAc                                                    >  1:206840/1‑150 (MQ=255)
                                                                                                     ttgAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGt                                                  >  2:637349/1‑150 (MQ=255)
                                                                                                     ttgAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTAACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGt                                                  <  2:1732997/150‑1 (MQ=255)
                                                                                                       gAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTGCATAACAAAAACGCAGCAACAAATTTACGTAt                                                >  2:1894436/1‑150 (MQ=255)
                                                                                                           cGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTAttttt                                            <  1:1755899/150‑1 (MQ=255)
                                                                                                           cGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTAttttt                                            <  2:1685454/150‑1 (MQ=255)
                                                                                                              gtCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTaa                                         >  2:819888/1‑150 (MQ=255)
                                                                                                               tCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAAc                                        >  2:1169032/1‑150 (MQ=255)
                                                                                                                ccGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAAca                                       >  2:572642/1‑150 (MQ=255)
                                                                                                                  gTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGGCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAAcatc                                     <  2:191403/150‑1 (MQ=255)
                                                                                                                   ttGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTATTGATATGTGACACGCTTTTGTCATTCCATAACAAAACAGCAGCAAAAAATTTACGTATTTTTAAAcatca                                    >  1:1013321/1‑150 (MQ=255)
                                                                                                                      tgAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTg                                 >  1:1274689/1‑150 (MQ=255)
                                                                                                                       taaGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGt                                <  2:2090817/149‑1 (MQ=255)
                                                                                                                          tcAATGGAAAAAATACGGGTCTATTATGACAGGAAATTGTGTCGATTCATCCCCCAAAAAGAGAGTAATTTCTTGATCTGTGAAAAGCTTTTGTCATTCCAAAACAAAAACTCATGAACAAAATTACGTATTTTTTAACATCATTGTAGc                             >  1:1111119/2‑150 (MQ=255)
                                                                                                                          gCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGc                             <  2:217940/150‑1 (MQ=255)
                                                                                                                            aaTGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAg                           >  1:1581358/1‑150 (MQ=255)
                                                                                                                            aaTGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAAAAAAAACGCAGAAACAAATTTACGTATTTTTTAACATCATTGTAGCat                           >  2:1386364/1‑149 (MQ=255)
                                                                                                                              tGGAATAAATACGGGTCTATTTTGACAGGAATTTGTGTAGATTACTCACCCCAAAAGAGAGTATTATCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACTCAGCAACAAATATACGTATTATTTAACATCATTGTAGAAGGt                         >  2:343560/1‑150 (MQ=255)
                                                                                                                               ggAAAAAATACGTCTCTATTTTGACAGGAATTTGTGTCGATTCCACCCCCCAAAATACAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAAAGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTg                        <  2:1278142/150‑1 (MQ=255)
                                                                                                                               ggAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGCCACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTAAGTATTTTTTAACATCATTGTAGCAGGTg                        >  1:1486931/1‑150 (MQ=255)
                                                                                                                               ggAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTg                        >  2:1657263/1‑150 (MQ=255)
                                                                                                                               ggAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTg                        <  2:1171169/150‑1 (MQ=255)
                                                                                                                               ggAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTg                        <  2:1427602/150‑1 (MQ=255)
                                                                                                                                    aaaTACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGAtttt                   >  2:678691/1‑150 (MQ=255)
                                                                                                                                       tACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGAttttttt                <  2:637420/150‑1 (MQ=255)
                                                                                                                                        aCGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTc               <  1:216934/150‑1 (MQ=255)
                                                                                                                                        aCGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTc               <  1:1684426/150‑1 (MQ=255)
                                                                                                                                        aCGGGTCTATTTTAACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTc               <  2:531044/150‑1 (MQ=255)
                                                                                                                                         cGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCa              <  1:1691434/150‑1 (MQ=255)
                                                                                                                                           ggTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAgg            >  2:1940418/1‑150 (MQ=255)
                                                                                                                                            gTCTATTTTGACAGGAATTTGTGTTGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGc           <  2:675476/150‑1 (MQ=255)
                                                                                                                                              cTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTGGAACTGTGCCACGCTTATGGCATTCAATAACAAAAACGCCGCAACAAATATACGTATTTTTTAACATCATTCTAGCAGGAGATTTTTATCAGGCGa         >  1:150243/1‑150 (MQ=255)
                                                                                                                                               tATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGAAGCAACATATTTACGTATTTTTTAACATCATTGTAGGAGGTGATTTTTTTCAGGCGat        >  1:300475/1‑150 (MQ=255)
                                                                                                                                                aTTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGatt       >  2:1737304/1‑150 (MQ=255)
                                                                                                                                                 ttttGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGatta      >  2:80649/1‑150 (MQ=255)
                                                                                                                                                   ttGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGattatt    <  1:952794/150‑1 (MQ=255)
                                                                                                                                                   ttGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGattatt    <  1:504914/150‑1 (MQ=255)
                                                                                                                                                     gACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCAATCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTtg  <  2:275315/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACAAAAGTTTCTTCACATTAGTTTACATAATATCAACACCATTAGCATTTAATGCCCTTTCACCCCAGATCCTTGACGACTCCAGGATAATTAGATGTTGTTGAATCGTGTCCGTTGTGAAGCAATGGAAAAAATACGGGTCTATTTTGACAGGAATTTGTGTCGATTCCTCCCCCCAAAAGAGAGTATTTTCTTGATCTGTGACACGCTTTTGTCATTCCATAACAAAAACGCAGCAACAAATTTACGTATTTTTTAACATCATTGTAGCAGGTGATTTTTTTCAGGCGATTATTTG  >  NC_000913/2405240‑2405538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: