Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,477,022 T→G 7.0% D203A (GAC→GCC)  dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,0220TG 7.0% 169.2 / 3.2 67D203A (GAC→GCC) dsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (9/52);  new base G (2/3);  total (11/56)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

CGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTA  >  NC_000913/2476874‑2477167
                                                                                                                                                    |                                                                                                                                                 
cGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCtgtctgtc                                                                                                                                                  <  2:1679906/150‑1 (MQ=255)
      cccAATATGATTCATCGCTGCAATAACAGCTAAAACAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAg                                                                                                                                            <  2:1381641/150‑1 (MQ=255)
         aaTATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCaa                                                                                                                                         <  1:1147577/150‑1 (MQ=255)
          atatGATTCATCGCCGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGACAGACAGGCATGGAGCAAc                                                                                                                                        <  1:1538550/150‑1 (MQ=255)
              gATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTAc                                                                                                                                    <  2:684918/150‑1 (MQ=255)
                 tCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCCTGCCGGTATGTCATGGAGCAAAGtcaagt                                                                                                                                 >  2:78567/1‑145 (MQ=255)
                  cATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTg                                                                                                                                <  1:810276/150‑1 (MQ=255)
                          cAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGt                                                                                                                        <  1:491933/150‑1 (MQ=255)
                            aTAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGGCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTa                                                                                                                      <  2:1421375/150‑1 (MQ=255)
                            aTAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTa                                                                                                                      <  1:1190084/150‑1 (MQ=255)
                             tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                     <  1:953029/150‑1 (MQ=255)
                             tAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTaa                                                                                                                     <  1:576834/150‑1 (MQ=255)
                                   cTAAATCAGAACGATCAAAGCCAATACCAGAAGATCGCGGCAAAATAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCg                                                                                                               <  2:1964571/150‑1 (MQ=255)
                                     aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTTCAACATACCAGTGTGTGATTGAGCCACCTACAGTTACACACGTTAATTaaccgg                                                                                                             >  1:1435085/1‑145 (MQ=255)
                                     aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACCCAGGTTAATTACCgtg                                                                                                             >  2:296734/1‑150 (MQ=255)
                                     aaaTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCgtg                                                                                                             <  1:436089/150‑1 (MQ=255)
                                       aTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGtt                                                                                                           <  1:298769/150‑1 (MQ=255)
                                       aTCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAAGGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGtt                                                                                                           <  1:78567/150‑1 (MQ=255)
                                       aTCAGAACGATCAAAGCCAATTCAAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCCAGCCTGTCGGGCCAGGGGCAACCTACACAGACCCCCGGTAAATACCGGGtt                                                                                                           >  1:106194/1‑150 (MQ=255)
                                       aTCAGAACGATCAAAGCCAATTACAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGtt                                                                                                           <  1:1272223/150‑1 (MQ=255)
                                        tCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTg                                                                                                          <  2:2053447/150‑1 (MQ=255)
                                           gAACGATCAAAGCCAATTCGAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCTTGTTGGtt                                                                                                       <  2:1629752/150‑1 (MQ=255)
                                           gAACGATCAAAGCCAATTCCAGAAGATGTAGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTTGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGtt                                                                                                       <  2:1149163/150‑1 (MQ=255)
                                               gATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAg                                                                                                   <  1:105717/150‑1 (MQ=255)
                                                aTCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCGGGCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGc                                                                                                  <  1:144364/150‑1 (MQ=255)
                                                  aaaatacAATTCAAGAAGTTGTCGTCAATTTACCGGAATAATGTTGCGCCAAACTATTACATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCAt                                                                                                <  2:1444108/144‑1 (MQ=255)
                                                   aaaGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATg                                                                                               <  2:653012/150‑1 (MQ=255)
                                                    aacccAATTCCAGAAGATGTCGGCAAATTAACGGAATAATGTTGCGCCCAACTTTGCCATTCATCAGTACCGGAGTCGTTGCTCCATGCCTGTCTGGCATGGAGCAACGTACTGTGACACATGTTAATTACCGTGTTGGTTAAAGCATGt                                                                                              <  1:1256297/147‑1 (MQ=255)
                                                       ccAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCtt                                                                                           <  2:2088170/150‑1 (MQ=255)
                                                         aaTTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTg                                                                                         >  2:1244956/1‑150 (MQ=255)
                                                               cgaagaaGTCGGCAAATTAACCGCATAATGATGCGCCCAACTATGCCATTCATCCGAACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTa                                                                                   <  1:1623843/149‑1 (MQ=255)
                                                               agaagaTGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTa                                                                                   <  1:5370/150‑1 (MQ=255)
                                                                         ggCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTa                                                                         <  2:1989353/150‑1 (MQ=255)
                                                                           cAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACa                                                                       <  2:970321/150‑1 (MQ=255)
                                                                                       aTAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAt                                                           <  2:781494/150‑1 (MQ=255)
                                                                                        tAATGTTGCGGCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                          <  1:1441319/150‑1 (MQ=255)
                                                                                        tAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGAtt                                                          <  2:652957/150‑1 (MQ=255)
                                                                                          aTGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttc                                                        <  2:1510429/150‑1 (MQ=255)
                                                                                            gTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATttctt                                                      <  1:1398650/150‑1 (MQ=255)
                                                                                              tGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTca                                                    <  1:1935741/150‑1 (MQ=255)
                                                                                                 gcCCAACTATGCCTTTGATCCGTAGCGGTGTCGTTTCTCGATGCCTGTCTGCCATGGAGCATCGTACAGTGACACAGGTTAATTACCGTGTTGGTTATAGCATGTGTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatcc                                                 <  2:1763266/150‑1 (MQ=255)
                                                                                                    cAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTcatccatc                                              <  1:1336433/150‑1 (MQ=255)
                                                                                                     aaCTAAGCAATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCa                                             <  1:760513/150‑1 (MQ=255)
                                                                                                      aCTATGCCATTCATCCGTACCGGAGTAGTTGCTCCAAGCCTGTCTGTCCTGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTTTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAg                                            <  1:84610/150‑1 (MQ=255)
                                                                                                            ccATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACTCAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtg                                      <  1:1244956/150‑1 (MQ=255)
                                                                                                            ccATTCATCCGTACCGGAGTCGTTGCACCATGCCTGTCTGTCATGGAGCAACGTACACTGACACAAGTTAAATACCGTGTTGGTTAAAGCATGTCTTTTAGCGTATTACCGGCAACATAATGGCAGGATTTATTCATCCATCAGAAAGtg                                      >  2:75568/1‑150 (MQ=255)
                                                                                                              aTTCATCCGTACCGGAGTCGGTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGTTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCAGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgat                                    <  1:1609135/150‑1 (MQ=255)
                                                                                                               ttCCTCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTTAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                                   <  1:360973/150‑1 (MQ=255)
                                                                                                               ttCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGtgatg                                   <  2:1435085/150‑1 (MQ=255)
                                                                                                                 cATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgag                                 <  1:436677/150‑1 (MQ=255)
                                                                                                                 cATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGAtgag                                 <  1:664111/150‑1 (MQ=255)
                                                                                                                      gTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATgagtgagt                            <  2:1712246/150‑1 (MQ=255)
                                                                                                                           ggAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                       <  2:894889/150‑1 (MQ=255)
                                                                                                                           ggAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                       <  2:902503/150‑1 (MQ=255)
                                                                                                                           ggAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGGTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTgcgc                       <  2:1039748/150‑1 (MQ=255)
                                                                                                                             aGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTg                     <  2:1857810/150‑1 (MQ=255)
                                                                                                                                cGTTGCTCCATGACAGACAGGCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACg                  >  1:1701080/1‑150 (MQ=255)
                                                                                                                                   tGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGgcg               <  1:1588982/150‑1 (MQ=255)
                                                                                                                                     cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGca             <  1:1299321/150‑1 (MQ=255)
                                                                                                                                       ccATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGCCGGCGcatc           >  2:388354/1‑150 (MQ=255)
                                                                                                                                       ccATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatc           >  1:452110/1‑150 (MQ=255)
                                                                                                                                       ccATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatc           >  1:933455/1‑150 (MQ=255)
                                                                                                                                       ccATGCCTGGCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatc           <  2:718332/150‑1 (MQ=255)
                                                                                                                                        cATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTGGGTTACAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCATCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGcatca          <  2:1015462/150‑1 (MQ=255)
                                                                                                                                         aTGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTAAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCCGTCAGCGcaactc         <  2:671372/150‑5 (MQ=255)
                                                                                                                                          tGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCatcatc        <  2:1766013/150‑1 (MQ=255)
                                                                                                                                           gCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGGCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCAtcatca       <  2:1002241/150‑1 (MQ=255)
                                                                                                                                            ccTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCaa      >  1:822451/1‑150 (MQ=255)
                                                                                                                                                gctgtgATGGAGCAACGTACAGTGACACAGGGTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTa  <  2:374331/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCCACCCCAATATGATTCATCGCGGCAATAACAGCTAAATCAGAACGATCAAAGCCAATTCCAGAAGATGTCGGCAAATTAACCGCATAATGTTGCGCCCAACTATGCCATTCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTA  >  NC_000913/2476874‑2477167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: