Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,483,900 T→A 5.5% V49D (GTC→GAC)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,9000TA 5.5% 293.5 / 5.1 102V49D (GTC→GAC) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (41/55);  new base A (3/3);  total (44/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 104 total aligned reads displayed.

ATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGT  >  NC_000913/2483751‑2484048
                                                                                                                                                     |                                                                                                                                                    
atCTATGAACGCAATAATTATTGATGACCCTCCTCTTGCTCTCGCAGCAATTCGTAATTTATTGATCTAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGGGCCGGTCAGCGGGTGGAAACACTTAAGCCTGATATCGt                                                                                                                                                      <  1:1010075/150‑1 (MQ=255)
     tGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAAATCGTAAATTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGATGAACTGCAGTACAGCGGTTGGAAACACTTAATCCTAATATCGtcatca                                                                                                                                                 >  2:969607/1‑150 (MQ=255)
        aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                                                              <  1:1136851/150‑1 (MQ=255)
        aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                                                              <  2:547228/150‑1 (MQ=255)
        aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                                                              <  2:334178/150‑1 (MQ=255)
        aCGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTg                                                                                                                                              <  2:1792766/150‑1 (MQ=255)
         ttcactaatTATTGATGACCATCCTCTAGCTATCCCACCAATTCGTAATTTATTGAGCTAAAACGATATTGAAATCTTAGCAGAGTTGACTGATGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGa                                                                                                                                             <  1:358626/145‑1 (MQ=255)
            aataatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                                                                          <  1:1587898/150‑1 (MQ=255)
             ataatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                                                                         <  2:1761127/150‑1 (MQ=255)
               aatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                                                                       >  2:649316/1‑150 (MQ=255)
                   attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATc                                                                                                                                   <  1:424203/150‑1 (MQ=255)
                   attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGAGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatata                                                                                                                                   >  1:150966/1‑149 (MQ=255)
                    ttGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcc                                                                                                                                  >  2:68273/1‑150 (MQ=255)
                     tgatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcag                                                                                                                                 <  1:1022096/150‑3 (MQ=255)
                      gatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                                                                <  1:1317622/150‑4 (MQ=255)
                      gatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                                                                <  2:810833/150‑4 (MQ=255)
                       atgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAATACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                                                                                               >  2:1842530/1‑150 (MQ=255)
                        tgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                                                                              >  1:1751003/1‑150 (MQ=255)
                         gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                             >  2:1167376/1‑150 (MQ=255)
                         gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                             <  1:1838739/150‑1 (MQ=255)
                         gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                             >  2:913645/1‑150 (MQ=255)
                          acactCCTCTTGCTATCGCAGCAATTCGTAATTTATTGAACAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCACCGGAg                                                                                                                            <  2:1025217/146‑1 (MQ=255)
                          aCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGAGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                                                                                            <  1:215931/150‑1 (MQ=255)
                           ccATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                                                           >  1:1934261/1‑150 (MQ=255)
                              tCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGCTGGAAACACTTAAGCCTGATATCGTCATCTTTGATGTCGATATCCCCGGAGTTaa                                                                                                                        >  1:1519616/1‑150 (MQ=255)
                                 tctTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                                                                                     <  1:829952/150‑1 (MQ=255)
                                  ctTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt                                                                                                                    <  2:777602/150‑1 (MQ=255)
                                     gCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATTTCGATATCCCCGGAGTTAACGGTAta                                                                                                                 >  2:900852/1‑149 (MQ=255)
                                      cTATCGCAGCAATTCGTAATTTATTGCTCACAACCGATCTTGAAAGCTTAGCCGCGTTGACTGAAGGCGTAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATcc                                                                                                                <  2:1010842/150‑1 (MQ=255)
                                       tATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa                                                                                                               <  1:118142/150‑1 (MQ=255)
                                        aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                              <  1:1453275/150‑1 (MQ=255)
                                        aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                              <  2:614442/150‑1 (MQ=255)
                                        aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                              >  1:536159/1‑150 (MQ=255)
                                           gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                           <  1:1481126/150‑1 (MQ=255)
                                           gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                           <  1:1954538/150‑1 (MQ=255)
                                           gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                           >  2:916493/1‑150 (MQ=255)
                                            cagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                                                                                          <  1:1340268/150‑1 (MQ=255)
                                                     ggaaTATATAGATCAAAAACGATATTGAAATCTTAGCACAGGTGCCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACAATTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTCTCCAGGTGTTAGAAACGc                                                                                                 <  2:416646/148‑1 (MQ=255)
                                                     gTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAActc                                                                                                 <  2:1654975/150‑3 (MQ=255)
                                                     gTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                                                                 <  2:468127/150‑1 (MQ=255)
                                                        aTTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                                                                                              <  2:748998/150‑1 (MQ=255)
                                                        aTTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                                                                                              <  1:234/150‑1 (MQ=255)
                                                             ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                                                                         >  1:1493838/1‑150 (MQ=255)
                                                               gATCAAAAACGATATTGAAATCTTAGCAGAGTTTACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                                                                       >  2:104202/1‑150 (MQ=255)
                                                               gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                                                                       >  2:2017165/1‑150 (MQ=255)
                                                                aTCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATGTCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcgc                                                                                      >  1:880458/1‑150 (MQ=255)
                                                                  cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCa                                                                                    >  2:1929815/1‑150 (MQ=255)
                                                                  cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCa                                                                                    >  2:1683101/1‑150 (MQ=255)
                                                                   aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                                                                   <  1:480386/150‑1 (MQ=255)
                                                                   aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                                                                   >  1:475183/1‑150 (MQ=255)
                                                                     aaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata                                                                                 <  2:179550/150‑1 (MQ=255)
                                                                             ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGaa                                                                         >  2:432535/1‑150 (MQ=255)
                                                                               gAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAatt                                                                       >  1:952039/1‑150 (MQ=255)
                                                                                aaaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAatta                                                                      >  1:1669906/1‑150 (MQ=255)
                                                                                   tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                                                                   <  1:943586/150‑1 (MQ=255)
                                                                                   tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                                                                   <  1:681442/150‑1 (MQ=255)
                                                                                        gCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTTACGGTGTCCAGGTGTTAGAAACGCTGAGGAAGCGCCATTATAGCGGAattattattttg                                                              >  2:1219709/1‑147 (MQ=255)
                                                                                         cAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                                                             <  2:1848660/150‑1 (MQ=255)
                                                                                              ttGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc                                                        <  1:1705660/150‑1 (MQ=255)
                                                                                              ttGACCGAAGGCGGAAGCGCCGGTCAGCGGGTGGAAACACTTCCGCCTGATATCGTCATCATTCATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATCGCGGAATTATTATTATCGTCTcc                                                        <  2:1445657/150‑1 (MQ=255)
                                                                                               tGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg                                                       <  2:827600/150‑1 (MQ=255)
                                                                                                 aCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCt                                                     >  1:524503/1‑150 (MQ=255)
                                                                                                   tGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaa                                                   <  2:488061/150‑1 (MQ=255)
                                                                                                    gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaa                                                  >  2:1595650/1‑150 (MQ=255)
                                                                                                    gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaa                                                  >  1:138323/1‑150 (MQ=255)
                                                                                                     aaGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaa                                                 >  2:1532234/1‑150 (MQ=255)
                                                                                                      aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaaa                                                >  1:643461/1‑150 (MQ=255)
                                                                                                      aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaaa                                                <  1:862527/150‑1 (MQ=255)
                                                                                                        gCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATg                                              >  2:1817283/1‑150 (MQ=255)
                                                                                                           gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGAcc                                           <  2:1934261/150‑1 (MQ=255)
                                                                                                            aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCa                                          <  1:626641/150‑1 (MQ=255)
                                                                                                               tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAttt                                       >  1:1316812/1‑150 (MQ=255)
                                                                                                                gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAtttt                                      <  1:467876/150‑1 (MQ=255)
                                                                                                                 ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAttttt                                     >  1:705493/1‑150 (MQ=255)
                                                                                                                  cggtCAGCGGGTGGAATCACGTAAGCCTGATATCGTCATCATTGATGTAGATATCCCCGTATTTAACGGTATCCAGGTGTTAGAAAATCTGATGAAGCTACAATATATCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTa                                    <  1:138916/147‑1 (MQ=255)
                                                                                                                    ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCTTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACg                                  <  2:1701169/150‑1 (MQ=255)
                                                                                                                    ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGGTCATTTTTagc                                  <  2:1029691/150‑3 (MQ=255)
                                                                                                                     tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCAGTGCTGTCGATATCCCCGGAGTTAACTGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTCTTATCGTCTCCGCTAAAAATGGTCATTTTTagcg                                 <  2:1130125/150‑4 (MQ=255)
                                                                                                                       aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGa                               >  2:443145/1‑150 (MQ=255)
                                                                                                                           ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACa                           >  2:1773172/1‑150 (MQ=255)
                                                                                                                              ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                        <  2:883299/150‑1 (MQ=255)
                                                                                                                              ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                        <  2:1493838/150‑1 (MQ=255)
                                                                                                                              ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCAAGGTGTTAGAAACGCTGAGGAAGCGACAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                        >  1:1106278/1‑150 (MQ=255)
                                                                                                                               gAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtgt                       >  1:1626102/1‑150 (MQ=255)
                                                                                                                                 aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGc                     <  1:360099/150‑1 (MQ=255)
                                                                                                                                 aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCACGTAGTTAACTGTATCCAGGTGTTAGAAACGCTTAGGAAGCGCCAATATTGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGc                     <  1:215992/150‑1 (MQ=255)
                                                                                                                                  acacTTAACCCTCATATCGACATCATTGATGACGATATCCCCGTAATTAACTGTAACCAGTTTTAAAAAAAGCTTAGCAATCACAAATATAGCGGATTTATTATTATCGTCTCGGCTAAAAATGACCATTTTTACGGGATACTGTGTGCt                    >  2:1037950/1‑150 (MQ=255)
                                                                                                                                      ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGCGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATATTTACGGGAAACATTGTGCTGATg                <  1:104202/150‑1 (MQ=255)
                                                                                                                                      ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCTGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATg                >  1:1441562/1‑150 (MQ=255)
                                                                                                                                         ccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTg             >  2:813171/6‑150 (MQ=255)
                                                                                                                                          gCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCGGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTgg            <  2:420676/150‑1 (MQ=255)
                                                                                                                                              gATATCGACATCAATGATGACGATATCCCCGGAGTAAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATTGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCt        >  2:405455/1‑150 (MQ=255)
                                                                                                                                                 atCGTCATCATTGATGTCGATTTCCCCGGAGTTAACGGTATCCAGGTGTTAGAATCGCTGAGGAAGCGCCAATATAGAGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAAt     >  1:1347297/1‑150 (MQ=255)
                                                                                                                                                  tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATg    >  1:1074074/1‑150 (MQ=255)
                                                                                                                                                   cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATgg   <  1:968141/150‑1 (MQ=255)
                                                                                                                                                   cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATgg   <  1:68273/150‑1 (MQ=255)
                                                                                                                                                    gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATg                >  1:908150/1‑136 (MQ=255)
                                                                                                                                                    gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATg                <  2:908150/136‑1 (MQ=255)
                                                                                                                                                    gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGt  <  2:671618/150‑1 (MQ=255)
                                                                                                                                                    gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGt  <  2:18823/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATCTATGAACGCAATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGT  >  NC_000913/2483751‑2484048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: