Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,093 T→C 5.6% A133A (GCT→GCC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,0930TC 5.6% 358.3 / 3.4 124A133A (GCT→GCCaceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base T (49/64);  new base C (3/4);  total (53/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAG  >  NC_000913/125945‑126241
                                                                                                                                                    |                                                                                                                                                    
ctccTTCTCAGGCATAAGAGAAGAAAGAATCTTCACCGGCAGCAGCACCAGCCGCTGCGTCGGAAATAGAATTAAACGTTCCGGATATCGGGTGTGACGAAGTTGAACTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                                     <  2:1158991/148‑1 (MQ=255)
cACCTGCTCAGGCAGAAGATAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                                     <  2:84184/150‑1 (MQ=255)
cACCTGCTCAGGCAGAAGAGTAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                                     <  1:404973/150‑1 (MQ=255)
     gCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAATAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                                                                                                                                >  1:1101217/1‑150 (MQ=255)
          ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt                                                                                                                                           <  1:1160982/150‑1 (MQ=255)
           gCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                                                                                                                          <  1:2038687/150‑1 (MQ=255)
            cAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa                                                                                                                                         >  1:1072986/1‑150 (MQ=255)
              gaagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGTGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc                                                                                                                                       <  1:196781/150‑1 (MQ=255)
               aagagaagaAATAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                                                                                                                      <  2:349423/150‑1 (MQ=255)
                   taagaaagaAGCAGCTCCGGCAGCAGCACCAGCTGCTGCGGGGGCAAAAGACGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGt                                                                                                                                  <  2:865056/149‑1 (MQ=11)
                   gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGAGGAAGTTGAAGTAACCGAAATCCTGGTGAAAGTTGTAGATAAAGTTGAAGCTGAACAGTAGCTGATCACCGt                                                                                                                                  >  2:1486571/1‑150 (MQ=37)
                     agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                                                >  1:1312184/1‑150 (MQ=255)
                     agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                                                >  1:410401/1‑150 (MQ=255)
                     agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                                                <  1:748716/150‑1 (MQ=255)
                      gaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAGGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGa                                                                                                                               >  1:1595845/1‑150 (MQ=255)
                          gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                                                           <  1:1379282/150‑1 (MQ=255)
                          gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                                                           <  2:1317975/150‑1 (MQ=255)
                            agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGa                                                                                                                         >  1:2097681/1‑150 (MQ=255)
                             gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                                                        <  1:1393249/150‑1 (MQ=255)
                              cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                                                                                                                       <  2:1801531/150‑1 (MQ=255)
                                 cTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg                                                                                                                    >  1:1778676/1‑150 (MQ=255)
                                   ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt                                                                                                                  >  1:1198329/1‑150 (MQ=255)
                                         gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAGGCTTCTATg                                                                                                            >  2:1567912/1‑150 (MQ=255)
                                         gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg                                                                                                            <  1:1459948/150‑1 (MQ=255)
                                         gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg                                                                                                            <  2:1584062/150‑1 (MQ=255)
                                            gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGGGACGAAGTTGAAGTGACCGAAGTGCTGGTGAAAGTTGGCGATAAAGTTGCAGCTGAAGAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGat                                                                                                         >  2:596134/1‑149 (MQ=17)
                                                  gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg                                                                                                   >  1:359623/1‑150 (MQ=255)
                                                      cTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTc                                                                                               <  1:877995/150‑1 (MQ=255)
                                                       tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc                                                                                              >  2:307657/1‑150 (MQ=255)
                                                        gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg                                                                                             >  1:848145/1‑150 (MQ=255)
                                                          ggcggcAAAAGCCGTTAACGTTCCGGATATCGGCAGCGACGTAGTTGACGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACTAGGCTTCTTTGGAAGTTCCGGCTCCGtt                                                                                           <  2:2024653/150‑1 (MQ=21)
                                                          ggcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt                                                                                           <  1:652350/150‑1 (MQ=255)
                                                               cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGCCCGAAATCCTGGAGAAAGTTGGCGATAAAGATGAAGCTGAACAGACGCTGAACACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGACCCGAATGCTg                                                                                      >  1:1460712/1‑150 (MQ=18)
                                                                       gTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATAACCGAACGAGCCGACAAGGCATCTATGCAAGTTCCGGCTGCGTTTGCTGGCAACGTg                                                                              >  1:1611135/1‑150 (MQ=18)
                                                                        ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGa                                                                             <  1:1470028/150‑1 (MQ=255)
                                                                           aagtaccGGACATCGGCTCAGATGAAGTTGAAATGACCGAGATCCTGGTCAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGTCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAag                                                                          <  1:1194317/145‑1 (MQ=14)
                                                                            cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTGTGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCAACGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCGCCGTTTACAGGCGACGTGAAgga                                                                         >  1:1363178/1‑150 (MQ=14)
                                                                             gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag                                                                        <  1:650957/150‑1 (MQ=255)
                                                                             gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGACGAAAAAGTTGCAGCAGAAAAGTCGCTCATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCATGCTCCGTTTGCTGGCACCGTGATagag                                                                        >  1:1583610/1‑150 (MQ=18)
                                                                                ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc                                                                     <  2:1726345/150‑1 (MQ=255)
                                                                                ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCTACGTGAAGGAAATc                                                                     >  2:207070/1‑150 (MQ=18)
                                                                                     tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt                                                                <  2:1890601/150‑1 (MQ=255)
                                                                                       tCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa                                                              >  2:1316296/1‑150 (MQ=255)
                                                                                       tCGGCAGCGACGAAGTTGAAGGGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa                                                              <  2:1899357/150‑1 (MQ=255)
                                                                                         ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAAc                                                            >  2:1430709/1‑150 (MQ=255)
                                                                                          gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                                           >  2:522743/1‑150 (MQ=255)
                                                                                          gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                                           <  1:1242913/150‑1 (MQ=255)
                                                                                            aGCGACGAAGTTGAAGTGACCGAAATCCTGGTTAAATTTGGCTATAATGTTGAAGCGTATCTGTCTCTGTTCACCGTAGAAGGCGTCAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg                                                         <  1:867919/150‑1 (MQ=25)
                                                                                            aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg                                                         <  2:835615/150‑1 (MQ=255)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                                       >  1:112510/1‑150 (MQ=255)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAAAGACCTGAAAGTCAACGTtgg                                                       >  2:857107/1‑150 (MQ=17)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                                       <  1:2036614/150‑1 (MQ=255)
                                                                                              cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                                       >  1:545394/1‑150 (MQ=255)
                                                                                               gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGATGAAGCTGAACAGTAGCTGATCACCGTAGAAGGCGACAAGTCTACTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt                                                      >  2:457081/1‑150 (MQ=255)
                                                                                                   aaGTTGAAGTGACCGAAATCCTGCTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                                                  <  1:86520/150‑1 (MQ=255)
                                                                                                     gTTGAAGTGACCGAAATCATGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGAAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTTCTGGCACCGTGAAAGACATCAAAGTGAACGAGGGAGACaaa                                                >  2:534034/1‑150 (MQ=255)
                                                                                                      ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg                                               <  2:1072986/150‑1 (MQ=255)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtg                                             >  1:1754456/1‑150 (MQ=255)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCATCGATGGAAGGTCCGGCGCCGTTTGCTGGCGGCGTGAACGAGCTGAAAGGGTACGTGGGGGCAAAAgtg                                             >  2:1846477/1‑150 (MQ=17)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTc                                                                                                                <  2:455278/83‑1 (MQ=255)
                                                                                                        gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTc                                                                                                                >  1:455278/1‑83 (MQ=255)
                                                                                                         aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAGATTTGAAGGCGACAAGTCTTATATGGAAGATCAGTCTCAGTTTGATGGCACAGTTAAAGAGATAAAAGTGAACGTGGGTGACAAAgtgt                                            >  1:1169426/1‑150 (MQ=255)
                                                                                                         aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtgt                                            <  1:576569/150‑1 (MQ=255)
                                                                                                          aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                                           >  2:963378/1‑150 (MQ=255)
                                                                                                          aGTGACCGAAATCCTGGTGAAAGTAGGCGATAAAGTTGAAGCTGAACAGGCGCTGAGCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                                           <  1:1281909/150‑1 (MQ=255)
                                                                                                            tcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                                           <  1:1790154/130‑1 (MQ=14)
                                                                                                            tcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                                           >  2:1790154/3‑132 (MQ=14)
                                                                                                            tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTGTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa                                         >  1:1289291/1‑150 (MQ=255)
                                                                                                              aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAcc                                       <  1:592810/150‑1 (MQ=255)
                                                                                                                   aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc                                  >  2:1321781/1‑150 (MQ=255)
                                                                                                                       cTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACAGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGATCAGTTTGATGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGcgg                              >  1:2087744/1‑148 (MQ=255)
                                                                                                                        tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACAGTATAAGGCTACAAGCCTTCTATGGAAGTTCCGTCTCCGTTTGCTGGCACCGGGAAAGAGATCAACGTGCACGTGGGTGACGAAGTGTCTGCCGGCTCGcggg                             >  2:344474/1‑147 (MQ=255)
                                                                                                                           tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTa                          <  2:1988916/150‑1 (MQ=255)
                                                                                                                            gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt                         >  1:892420/1‑150 (MQ=255)
                                                                                                                              aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg                       <  1:1518942/150‑1 (MQ=255)
                                                                                                                               aGTTGGCGATAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt                      <  2:573452/150‑1 (MQ=255)
                                                                                                                                    gCGATAAAGTTGAAGCTGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCg                 <  1:1031734/150‑1 (MQ=255)
                                                                                                                                    gCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTTGAAGGCGACAAGGCTTCTATGGAAGTTCCGGATCCGTTTGCTGGCACCGTTAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACGGGCTCGCTGATTATGGTCTTCg                 <  1:368268/150‑1 (MQ=255)
                                                                                                                                    gCGAAAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCg                 <  1:8167/150‑1 (MQ=255)
                                                                                                                                     cGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGa                <  2:1595845/150‑1 (MQ=255)
                                                                                                                                       aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAg              <  1:1143116/150‑1 (MQ=255)
                                                                                                                                       aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAg              <  2:1214784/150‑1 (MQ=255)
                                                                                                                                       aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAg              >  1:747991/1‑150 (MQ=255)
                                                                                                                                          aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAGCGTGAAAGAGCTCAAAGTGACCGTTGGAGAGAAAGTGTAAACGGGCTCGCTGATTATGATCTTCGAAGttg           >  2:1380729/1‑148 (MQ=14)
                                                                                                                                          aaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc                                                                     <  2:126991/92‑1 (MQ=255)
                                                                                                                                          aaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc                                                                     >  1:126991/1‑92 (MQ=255)
                                                                                                                                            gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg         >  1:1183214/1‑150 (MQ=255)
                                                                                                                                            gTTGAAGCTGAACAGTCGCGGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGGGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg         <  1:1022251/150‑1 (MQ=255)
                                                                                                                                              tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg       >  2:936855/1‑150 (MQ=255)
                                                                                                                                              tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg       <  1:1617117/150‑1 (MQ=255)
                                                                                                                                               gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAATGAGATCAAAGTGAACGTGGGTGACTAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt      <  1:156847/150‑1 (MQ=255)
                                                                                                                                               gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt      <  1:896902/150‑1 (MQ=255)
                                                                                                                                               gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt      >  1:1110042/1‑150 (MQ=255)
                                                                                                                                               gAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt      <  1:1089012/150‑1 (MQ=255)
                                                                                                                                                caGCTGAACAGTCGCTGATCTCCTTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGGGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCTAAGTCGCGGGTg     <  2:1605091/149‑1 (MQ=255)
                                                                                                                                                aaGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGca                                                                                       >  2:1155329/1‑67 (MQ=255)
                                                                                                                                                 aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGa    <  2:1754456/150‑1 (MQ=255)
                                                                                                                                                  gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGaa   <  1:941024/150‑1 (MQ=255)
                                                                                                                                                   cTGAACCGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACGGTGCAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAg  <  2:1477004/150‑1 (MQ=255)
                                                                                                                                                   cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCGGTTTGCTGGCACCGTTAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGTCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAg  <  1:854321/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAG  >  NC_000913/125945‑126241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: