Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,483,912 T→A 5.2% V53D (GTC→GAC)  evgA → response regulator in two‑component regulatory system with EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,9120TA 5.2% 323.3 / 5.0 107V53D (GTC→GAC) evgAresponse regulator in two‑component regulatory system with EvgS
Reads supporting (aligned to +/- strand):  ref base T (43/58);  new base A (3/3);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 108 total aligned reads displayed.

AATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTAA  >  NC_000913/2483763‑2484059
                                                                                                                                                     |                                                                                                                                                   
aataatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGt                                                                                                                                                     <  1:1587898/150‑1 (MQ=255)
 ataatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTc                                                                                                                                                    <  2:1761127/150‑1 (MQ=255)
   aatTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGa                                                                                                                                                  >  2:649316/1‑150 (MQ=255)
       attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATc                                                                                                                                              <  1:424203/150‑1 (MQ=255)
       attGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGAGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGatata                                                                                                                                              >  1:150966/1‑149 (MQ=255)
        ttGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATcc                                                                                                                                             >  2:68273/1‑150 (MQ=255)
         tgatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcag                                                                                                                                            <  1:1022096/150‑3 (MQ=255)
          gatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                                                                           <  1:1317622/150‑4 (MQ=255)
          gatgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGACATCAATGATGACGATATcagg                                                                                                                                           <  2:810833/150‑4 (MQ=255)
           atgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAATACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCg                                                                                                                                          >  2:1842530/1‑150 (MQ=255)
            tgaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCgg                                                                                                                                         >  1:1751003/1‑150 (MQ=255)
             gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                                        <  1:1838739/150‑1 (MQ=255)
             gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                                        >  2:913645/1‑150 (MQ=255)
             gaCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGa                                                                                                                                        >  2:1167376/1‑150 (MQ=255)
              acactCCTCTTGCTATCGCAGCAATTCGTAATTTATTGAACAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCACCGGAg                                                                                                                                       <  2:1025217/146‑1 (MQ=255)
              aCCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGAGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAg                                                                                                                                       <  1:215931/150‑1 (MQ=255)
               ccATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGTAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                                                                      >  1:1845172/1‑150 (MQ=255)
               ccATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGt                                                                                                                                      >  1:1934261/1‑150 (MQ=255)
                  tCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGCTGGAAACACTTAAGCCTGATATCGTCATCTTTGATGTCGATATCCCCGGAGTTaa                                                                                                                                   >  1:1519616/1‑150 (MQ=255)
                     tctTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACgg                                                                                                                                <  1:829952/150‑1 (MQ=255)
                      ctTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGt                                                                                                                               <  2:777602/150‑1 (MQ=255)
                         gCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATTTCGATATCCCCGGAGTTAACGGTAta                                                                                                                            >  2:900852/1‑149 (MQ=255)
                          cTATCGCAGCAATTCGTAATTTATTGCTCACAACCGATCTTGAAAGCTTAGCCGCGTTGACTGAAGGCGTAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATcc                                                                                                                           <  2:1010842/150‑1 (MQ=255)
                           tATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCa                                                                                                                          <  1:118142/150‑1 (MQ=255)
                            aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                                         <  2:614442/150‑1 (MQ=255)
                            aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                                         >  1:536159/1‑150 (MQ=255)
                            aTCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAg                                                                                                                         <  1:1453275/150‑1 (MQ=255)
                               gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                                      >  2:916493/1‑150 (MQ=255)
                               gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                                      <  1:1481126/150‑1 (MQ=255)
                               gcagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtg                                                                                                                      <  1:1954538/150‑1 (MQ=255)
                                cagcaATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGgtgt                                                                                                                     <  1:1340268/150‑1 (MQ=255)
                                         ggaaTATATAGATCAAAAACGATATTGAAATCTTAGCACAGGTGCCTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACAATTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTCTCCAGGTGTTAGAAACGc                                                                                                            <  2:416646/148‑1 (MQ=255)
                                         gTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAActc                                                                                                            <  2:1654975/150‑3 (MQ=255)
                                         gTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGc                                                                                                            <  2:468127/150‑1 (MQ=255)
                                            aTTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                                                                                                         <  1:234/150‑1 (MQ=255)
                                            aTTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGa                                                                                                         <  2:748998/150‑1 (MQ=255)
                                               tATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGa                                                                                                      <  1:969607/150‑1 (MQ=255)
                                                 ttGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAg                                                                                                    >  1:1493838/1‑150 (MQ=255)
                                                   gATCAAAAACGATATTGAAATCTTAGCAGAGTTTACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                                                                                  >  2:104202/1‑150 (MQ=255)
                                                   gATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcg                                                                                                  >  2:2017165/1‑150 (MQ=255)
                                                    aTCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATGTCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAgcgc                                                                                                 >  1:880458/1‑150 (MQ=255)
                                                      cAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCa                                                                                               >  2:1683101/1‑150 (MQ=255)
                                                       aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                                                                              >  1:475183/1‑150 (MQ=255)
                                                       aaaaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCaa                                                                                              <  1:480386/150‑1 (MQ=255)
                                                         aaaCGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAata                                                                                            <  2:179550/150‑1 (MQ=255)
                                                            cGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAg                                                                                         <  2:1956564/150‑1 (MQ=255)
                                                                 ttGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGaa                                                                                    >  2:432535/1‑150 (MQ=255)
                                                                    aaaTCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAatta                                                                                 >  1:1669906/1‑150 (MQ=255)
                                                                       tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                                                                              <  1:943586/150‑1 (MQ=255)
                                                                       tCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAattatta                                                                              <  1:681442/150‑1 (MQ=255)
                                                                            gCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTTACGGTGTCCAGGTGTTAGAAACGCTGAGGAAGCGCCATTATAGCGGAattattattttg                                                                         >  2:1219709/1‑147 (MQ=255)
                                                                             cAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCg                                                                        <  2:1848660/150‑1 (MQ=255)
                                                                                  ttGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTcc                                                                   <  1:1705660/150‑1 (MQ=255)
                                                                                  ttGACCGAAGGCGGAAGCGCCGGTCAGCGGGTGGAAACACTTCCGCCTGATATCGTCATCATTCATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATCGCGGAATTATTATTATCGTCTcc                                                                   <  2:1445657/150‑1 (MQ=255)
                                                                                   tGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCg                                                                  <  2:827600/150‑1 (MQ=255)
                                                                                       tGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaa                                                              <  2:488061/150‑1 (MQ=255)
                                                                                        gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaa                                                             >  2:1595650/1‑150 (MQ=255)
                                                                                        gAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaa                                                             >  1:138323/1‑150 (MQ=255)
                                                                                          aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaaa                                                           >  1:643461/1‑150 (MQ=255)
                                                                                          aGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTaaaaa                                                           <  1:862527/150‑1 (MQ=255)
                                                                                            gCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATg                                                         >  2:1817283/1‑150 (MQ=255)
                                                                                               gAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGAcc                                                      <  2:1934261/150‑1 (MQ=255)
                                                                                                aaGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCa                                                     <  1:626641/150‑1 (MQ=255)
                                                                                                   tGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAttt                                                  >  1:1316812/1‑150 (MQ=255)
                                                                                                    gCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAtttt                                                 <  1:467876/150‑1 (MQ=255)
                                                                                                     ccGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCAttttt                                                >  1:705493/1‑150 (MQ=255)
                                                                                                      cggtCAGCGGGTGGAATCACGTAAGCCTGATATCGTCATCATTGATGTAGATATCCCCGTATTTAACGGTATCCAGGTGTTAGAAAATCTGATGAAGCTACAATATATCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTa                                               <  1:138916/147‑1 (MQ=255)
                                                                                                        ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCTTTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACg                                             <  2:1701169/150‑1 (MQ=255)
                                                                                                        ttCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGGTCATTTTTagc                                             <  2:1029691/150‑3 (MQ=255)
                                                                                                         tCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCAGTGCTGTCGATATCCCCGGAGTTAACTGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTCTTATCGTCTCCGCTAAAAATGGTCATTTTTagcg                                            <  2:1130125/150‑4 (MQ=255)
                                                                                                           aGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGa                                          >  2:443145/1‑150 (MQ=255)
                                                                                                               ggTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACa                                      >  2:1773172/1‑150 (MQ=255)
                                                                                                                  ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                                   <  2:883299/150‑1 (MQ=255)
                                                                                                                  ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                                   <  2:1493838/150‑1 (MQ=255)
                                                                                                                  ggAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCAAGGTGTTAGAAACGCTGAGGAAGCGACAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtg                                   >  1:1106278/1‑150 (MQ=255)
                                                                                                                   gAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATtgt                                  >  1:1626102/1‑150 (MQ=255)
                                                                                                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGc                                <  1:360099/150‑1 (MQ=255)
                                                                                                                     aaCACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCACGTAGTTAACTGTATCCAGGTGTTAGAAACGCTTAGGAAGCGCCAATATTGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGc                                <  1:215992/150‑1 (MQ=255)
                                                                                                                      acacTTAACCCTCATATCGACATCATTGATGACGATATCCCCGTAATTAACTGTAACCAGTTTTAAAAAAAGCTTAGCAATCACAAATATAGCGGATTTATTATTATCGTCTCGGCTAAAAATGACCATTTTTACGGGATACTGTGTGCt                               >  2:1037950/1‑150 (MQ=255)
                                                                                                                          ttAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGCGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATATTTACGGGAAACATTGTGCTGATg                           <  1:104202/150‑1 (MQ=255)
                                                                                                                             ccggggATATCGACATCAATGATGACGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTg                        >  2:813171/6‑150 (MQ=255)
                                                                                                                              gCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCGGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTgg                       <  2:420676/150‑1 (MQ=255)
                                                                                                                                  gATATCGACATCAATGATGACGATATCCCCGGAGTAAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATTGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCt                   >  2:405455/1‑150 (MQ=255)
                                                                                                                                     atCGTCATCATTGATGTCGATTTCCCCGGAGTTAACGGTATCCAGGTGTTAGAATCGCTGAGGAAGCGCCAATATAGAGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAAt                >  1:1347297/1‑150 (MQ=255)
                                                                                                                                      tCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATg               >  1:1074074/1‑150 (MQ=255)
                                                                                                                                       cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATgg              <  1:968141/150‑1 (MQ=255)
                                                                                                                                       cGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATgg              <  1:68273/150‑1 (MQ=255)
                                                                                                                                        gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATg                           >  1:908150/1‑136 (MQ=255)
                                                                                                                                        gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATg                           <  2:908150/136‑1 (MQ=255)
                                                                                                                                        gTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGt             <  2:18823/150‑1 (MQ=255)
                                                                                                                                          catcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGttt           <  2:1751003/150‑1 (MQ=255)
                                                                                                                                            tcatTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCg         <  1:498431/150‑1 (MQ=255)
                                                                                                                                             catTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGt        >  2:819376/1‑150 (MQ=255)
                                                                                                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAACCGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTg       <  2:1259085/150‑1 (MQ=255)
                                                                                                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCGATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTg       <  1:1852163/150‑1 (MQ=255)
                                                                                                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTg       <  1:762239/150‑1 (MQ=255)
                                                                                                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTg       <  1:443145/150‑1 (MQ=255)
                                                                                                                                              atTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTg       <  1:1419669/150‑1 (MQ=255)
                                                                                                                                                 gATGTCGATATCCCCGGAGTTAACGGTATCCTGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGt    >  1:1267411/1‑150 (MQ=255)
                                                                                                                                                   tGTCGATATCCCCGGAGTTACCGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTaa  <  2:240785/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AATAATTATTGATGACCATCCTCTTGCTATCGCAGCAATTCGTAATTTATTGATCAAAAACGATATTGAAATCTTAGCAGAGTTGACTGAAGGCGGAAGTGCCGTTCAGCGGGTGGAAACACTTAAGCCTGATATCGTCATCATTGATGTCGATATCCCCGGAGTTAACGGTATCCAGGTGTTAGAAACGCTGAGGAAGCGCCAATATAGCGGAATTATTATTATCGTCTCCGCTAAAAATGACCATTTTTACGGGAAACATTGTGCTGATGCTGGCGCTAATGGTTTCGTGAGTAA  >  NC_000913/2483763‑2484059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: