Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,222 T→G 6.4% intergenic (+17/+35) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2220TG 6.4% 358.3 / 6.9 120intergenic (+17/+35)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (48/62);  new base G (6/2);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGC  >  NC_000913/2519073‑2519370
                                                                                                                                                     |                                                                                                                                                    
cccGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAATAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCGTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGat                                                                                                                                                      >  1:579854/1‑150 (MQ=255)
 ccGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTAGCAATTTCCTCGCACGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGata                                                                                                                                                     >  2:1459574/1‑150 (MQ=255)
      gTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCTCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                                                                <  1:2000549/150‑1 (MQ=255)
      gTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCACGAtattata                                                                                                                                                >  1:668014/1‑149 (MQ=255)
      gTATCGACAAATCATCAACGAGATTTATAAATTGCCAGACATTGACCATGAAAAAGGCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATTTTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                                                                <  2:1914836/150‑1 (MQ=255)
           gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                           >  1:1610988/1‑150 (MQ=255)
            aCAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                                                                          <  2:496292/150‑1 (MQ=255)
             cAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGcc                                                                                                                                         <  1:995906/150‑1 (MQ=255)
               aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGt                                                                                                                                       <  1:273871/150‑1 (MQ=255)
                 tcatcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttt                                                                                                                                     <  1:889041/150‑1 (MQ=255)
                   atcaACGCGATAGATAATATGCCAGACATTGAGCTGGAAAAAGTAGCGCAATTTTTGGCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                   <  2:296077/150‑3 (MQ=255)
                     caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCTCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                 <  2:35350/150‑1 (MQ=255)
                     caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                 >  1:521835/1‑150 (MQ=255)
                      aaCGCTATAGAAAATATGACAGACATTGAGCAGGAAAAATTCGCCCAATTTTTGTCACGGGAAGGAATTCGCTATTTCCTCGCCCGTCTCGATATTGACGAATCAGCAAAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtt                                                                                                                                <  2:1991193/150‑1 (MQ=255)
                       aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                               <  1:1426355/150‑1 (MQ=255)
                       aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGAAATTTACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                               <  2:1570425/150‑1 (MQ=255)
                        cgcgATAGATAATATGGCAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                              <  2:1791055/150‑1 (MQ=255)
                        cgcgATAGATAATATGACAGACATTGAGAAGGATAAAGTAGCTCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATTAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                              <  2:1144597/150‑1 (MQ=255)
                         gcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                                                             <  2:2033671/150‑1 (MQ=255)
                          cgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                            <  2:2069166/150‑1 (MQ=255)
                              agataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTAACGTGAAGGAATTCGCAATTTCCTCGACCGTCTCGATATTGAAGAATCAGCATAAATAAAAAACGGCAGGAGAATATCTCCATCCGTTTATCTTTATACCCg                                                                                                                        >  1:157142/1‑150 (MQ=255)
                               gataATAAGACAGACATTCAGCAGGAAAAAGTCCCGCAATTATTGTCACGTGAAGGAATTCGCAATTTACTCGCCCGTCTCGATATTGACAAATCAGAATAAATAAAAAACGGCAGGATATTATATCCTGCCGTTTATATATTTACACGc                                                                                                                       >  2:261134/1‑150 (MQ=255)
                                      tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                >  1:871534/1‑150 (MQ=255)
                                      tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                <  2:868271/150‑1 (MQ=255)
                                      tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGACTCGATATTGACGAATCAGCATAAATAAAAAACGGCTGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTaa                                                                                                                >  1:1022683/1‑149 (MQ=255)
                                       gacatacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAct                                                                                                               <  2:575136/150‑1 (MQ=255)
                                          agacaTTGAGCAGTACAACGTCGCTCAATTTTTGGAACGTGAAGGAATTCGCAATTTCCTCGCTCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                            <  2:1314303/150‑1 (MQ=255)
                                          agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                            <  1:1219445/150‑1 (MQ=255)
                                          agacaTTGAGCACGAAAACGTCGCGCATTTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTTCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                            <  1:1127807/150‑1 (MQ=255)
                                           gacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg                                                                                                           >  1:1011815/1‑150 (MQ=255)
                                             caTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGACCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGACATAATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAt                                                                                                         >  1:241384/1‑150 (MQ=255)
                                              aTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAtt                                                                                                        <  1:1123055/150‑1 (MQ=255)
                                                tGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCAGTTTATCTTTTTACACGCTAATTACTGCTGAtttt                                                                                                      >  1:553402/1‑150 (MQ=255)
                                                 gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGCTGATTTTc                                                                                                     >  2:1267734/1‑150 (MQ=255)
                                                  aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                    <  1:7021/150‑1 (MQ=255)
                                                  aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                    <  1:1075532/150‑1 (MQ=255)
                                                  aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                    >  1:272647/1‑150 (MQ=255)
                                                   gCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGAAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgc                                                                                                   <  1:932531/150‑1 (MQ=255)
                                                    cAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATACGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                  <  1:1248153/150‑1 (MQ=255)
                                                     cggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                 <  2:1688085/149‑1 (MQ=255)
                                                     aGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                 <  1:475227/150‑1 (MQ=255)
                                                      ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTAAACGCTAATTAATCCTCATTTTCGCGtt                                                                                                >  2:669118/1‑150 (MQ=255)
                                                       gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc                                                                                               <  1:506636/150‑1 (MQ=255)
                                                        aaaaaGTCGCGCAATTTATGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGCATATTATCTACAGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                                                              >  2:587549/1‑150 (MQ=255)
                                                          acaGTCGCGCAATTTTTGTCAGGGGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  2:1405999/148‑1 (MQ=255)
                                                          aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  1:1245183/150‑1 (MQ=255)
                                                          aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  1:1970897/150‑1 (MQ=255)
                                                            aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCaa                                                                                          <  1:396394/150‑1 (MQ=255)
                                                             gTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAAt                                                                                         >  2:765366/1‑150 (MQ=255)
                                                                  gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                    <  2:1002881/150‑1 (MQ=255)
                                                                      tttttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATCTCCTGCCGTTTATCATTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCCAg                                                                                >  2:168862/1‑150 (MQ=255)
                                                                         ttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcgc                                                                             <  2:328048/150‑1 (MQ=255)
                                                                             cACGTGTAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttg                                                                         <  2:673315/150‑1 (MQ=255)
                                                                             cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtttt                                                                         >  1:1107878/1‑149 (MQ=255)
                                                                               cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                       <  2:2101541/150‑1 (MQ=255)
                                                                               cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCAGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                       >  2:1054659/1‑150 (MQ=255)
                                                                               cGTGAAGGAATTCGCAATTTCCTAGCCCGTCTCGATATTGAAGAATAAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                       <  1:130073/150‑1 (MQ=255)
                                                                                tttaaGGAATTCGCAATTTGATCGCCCGGCTCTATATTGACGAATCAGAATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATATTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgttg                                                                      <  1:1564139/147‑1 (MQ=255)
                                                                                  gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                    <  2:620912/150‑1 (MQ=255)
                                                                                     ggAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTGTGTTGATACg                                                                 <  1:1262014/150‑1 (MQ=255)
                                                                                        aTTCGCAATTTCATCGCCCGTCTCGATTTTGACGTATCAGCATAAATAAAAAACTGCAGGAAATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGGTTTGTTGATTTGCTc                                                              <  2:557000/150‑1 (MQ=255)
                                                                                           cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                           >  2:59458/1‑150 (MQ=255)
                                                                                            gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTCCAGCAATAAAATCCAGCGCTTTGGTGATACGCTCGATa                                                          >  2:1504475/1‑150 (MQ=255)
                                                                                            gCAATATCCTCGCCCGTCTCGATATTGACGAATCAGCATAAAAAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTCATTTTCGCGTTCAGCAATAAAATCCAGAGCTTTGTTGATACGCTCGATa                                                          >  1:43061/1‑150 (MQ=255)
                                                                                             cAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATaa                                                         >  2:1391966/1‑149 (MQ=255)
                                                                                             cAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc                                                         >  1:1141638/1‑150 (MQ=255)
                                                                                              aaTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTGCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACt                                                        <  1:1662281/150‑1 (MQ=255)
                                                                                                   ccTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg                                                   <  2:1816361/150‑1 (MQ=255)
                                                                                                    cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACACTCGATACTGCGGGt                                                  >  1:419874/1‑150 (MQ=255)
                                                                                                     tCGCCCGTCTCTATTTTGACGATTCAGCATTAATAAAAAACGGCAGGTTATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTTCTGCTGATTTTCGCGTTCAGCATTAATATCCTGCGCTTTGTTGATACGCTCGATACTGCGGGTc                                                 <  1:840565/150‑1 (MQ=255)
                                                                                                     tCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc                                                 <  2:585378/150‑1 (MQ=255)
                                                                                                       gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGAGTTCAGAAATAAAATCCATCGCTTTGTTGATACGCTCGATACTGAGGGTCtt                                               >  1:60591/1‑150 (MQ=255)
                                                                                                              tcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATTATCTCCTGCAGTTTATCTTGTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAAtt                                        >  1:806918/1‑150 (MQ=255)
                                                                                                               cGATATTGACGAATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGGTCAGCAATAAAATCCAGCGCGTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                       <  1:1282843/150‑1 (MQ=255)
                                                                                                               cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                       <  1:1365368/150‑1 (MQ=255)
                                                                                                                gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc                                      >  2:463402/1‑150 (MQ=255)
                                                                                                                 atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg                                     <  2:1018954/150‑1 (MQ=255)
                                                                                                                   atTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                   >  1:619855/1‑150 (MQ=255)
                                                                                                                     tGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGGTTATCTTTTTACACGCTAAGTACTGCTGAGTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTTCGGGTCTTACCAATTGCGTGaa                                 >  2:1498852/1‑150 (MQ=255)
                                                                                                                      gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGCAc                                >  1:46908/1‑150 (MQ=255)
                                                                                                                      gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAAc                                <  1:1164463/150‑1 (MQ=255)
                                                                                                                      gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAATGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCTATACTGAGGGTATTACAAATTGCGTGaaa                                >  1:255502/1‑149 (MQ=255)
                                                                                                                       aCGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                               <  1:490544/150‑1 (MQ=255)
                                                                                                                          aaTCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTa                            >  2:965989/1‑150 (MQ=255)
                                                                                                                            tCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGATGATTTTCGCGGTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTAGATACTGCGGGTCTAACCAATTGCGTGAACGGTAAc                          >  1:506420/1‑150 (MQ=255)
                                                                                                                              aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTCCCAATTGCGTGAACGGTAACAt                        >  2:1661576/1‑150 (MQ=255)
                                                                                                                               gCATAAATAAAAAACGGCAGGATATTTTCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc                       >  1:721418/1‑150 (MQ=255)
                                                                                                                                   aaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                   >  2:1235208/1‑150 (MQ=255)
                                                                                                                                   aaaagataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                   >  2:864739/8‑150 (MQ=39)
                                                                                                                                      taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCt                >  1:37540/1‑150 (MQ=255)
                                                                                                                                       aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGACCGGTAACATCCAGTGCTg               >  2:515271/1‑150 (MQ=255)
                                                                                                                                        aaaaaCTGCAGTATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCGAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTgg              <  1:1286707/150‑1 (MQ=255)
                                                                                                                                          aaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag            <  1:1739735/150‑1 (MQ=255)
                                                                                                                                          aaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag            <  2:92169/150‑1 (MQ=255)
                                                                                                                                           aaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga           <  1:707630/150‑1 (MQ=255)
                                                                                                                                           aaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTAAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCTTGCACGGTAACATCCAGTGCTGgata           >  2:1527252/1‑148 (MQ=255)
                                                                                                                                           aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga           >  2:1779106/1‑150 (MQ=255)
                                                                                                                                                cAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcc      >  2:1167237/1‑150 (MQ=255)
                                                                                                                                                    atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGc  <  1:464860/150‑1 (MQ=255)
                                                                                                                                                    atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGc  >  2:1668201/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCGGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGC  >  NC_000913/2519073‑2519370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: