Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,225 T→A 5.4% intergenic (+20/+32) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2250TA 5.4% 355.7 / 5.6 122intergenic (+20/+32)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (51/64);  new base A (5/2);  total (56/66)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGC  >  NC_000913/2519079‑2519370
                                                                                                                                                  |                                                                                                                                                 
gTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCTCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                                                                <  1:2000549/150‑1 (MQ=255)
gTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCACGAtattata                                                                                                                                                >  1:668014/1‑149 (MQ=255)
gTATCGACAAATCATCAACGAGATTTATAAATTGCCAGACATTGACCATGAAAAAGGCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATTTTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtc                                                                                                                                                <  2:1914836/150‑1 (MQ=255)
    cGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTcca                                                                                                                                            >  1:236564/1‑149 (MQ=255)
     gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                           >  1:1610988/1‑150 (MQ=255)
     caCAAATCATCAACCCGAAAGAAAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                           <  2:559158/149‑1 (MQ=255)
      aCAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                                                                          <  2:496292/150‑1 (MQ=255)
         aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGt                                                                                                                                       <  1:273871/150‑1 (MQ=255)
           tcatcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttt                                                                                                                                     <  1:889041/150‑1 (MQ=255)
             atcaACGCGATAGATAATATGCCAGACATTGAGCTGGAAAAAGTAGCGCAATTTTTGGCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGttttt                                                                                                                                   <  2:296077/150‑3 (MQ=255)
               caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                 >  1:521835/1‑150 (MQ=255)
                aaCGCTATAGAAAATATGACAGACATTGAGCAGGAAAAATTCGCCCAATTTTTGTCACGGGAAGGAATTCGCTATTTCCTCGCCCGTCTCGATATTGACGAATCAGCAAAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtt                                                                                                                                <  2:1991193/150‑1 (MQ=255)
                 aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                               <  1:1426355/150‑1 (MQ=255)
                 aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                               <  2:1481228/150‑1 (MQ=255)
                 aCGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGAAATTTACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttt                                                                                                                               <  2:1570425/150‑1 (MQ=255)
                  cgcgATAGATAATATGGCAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                              <  2:1791055/150‑1 (MQ=255)
                   gcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCttttt                                                                                                                             <  2:2033671/150‑1 (MQ=255)
                    cgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                            <  2:2069166/150‑1 (MQ=255)
                    cgATAGATAATATGAAAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTTTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTa                                                                                                                            <  2:1988362/150‑1 (MQ=255)
                        agataATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTAACGTGAAGGAATTCGCAATTTCCTCGACCGTCTCGATATTGAAGAATCAGCATAAATAAAAAACGGCAGGAGAATATCTCCATCCGTTTATCTTTATACCCg                                                                                                                        >  1:157142/1‑150 (MQ=255)
                         gataATAAGACAGACATTCAGCAGGAAAAAGTCCCGCAATTATTGTCACGTGAAGGAATTCGCAATTTACTCGCCCGTCTCGATATTGACAAATCAGAATAAATAAAAAACGGCAGGATATTATATCCTGCCGTTTATATATTTACACGc                                                                                                                       >  2:261134/1‑150 (MQ=255)
                              tatGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAAtt                                                                                                                  <  1:1203841/150‑1 (MQ=255)
                                tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                >  1:871534/1‑150 (MQ=255)
                                tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                <  2:868271/150‑1 (MQ=255)
                                tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGACTCGATATTGACGAATCAGCATAAATAAAAAACGGCTGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTaa                                                                                                                >  1:1022683/1‑149 (MQ=255)
                                 gacatacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAct                                                                                                               <  2:575136/150‑1 (MQ=255)
                                  acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACAAATCAGCATAAATAAAAAACGGCAGCATATTATCTCATGACGTTTATCTTTATACACGCTAATTActg                                                                                                              >  2:241244/1‑150 (MQ=255)
                                    agacaTTGAGCACGAAAACGTCGCGCATTTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTTCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                            <  1:1127807/150‑1 (MQ=255)
                                     gacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg                                                                                                           >  1:1011815/1‑150 (MQ=255)
                                       caTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGACCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGACATAATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGAt                                                                                                         >  1:241384/1‑150 (MQ=255)
                                           gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGCTGATTTTc                                                                                                     >  2:1267734/1‑150 (MQ=255)
                                            aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                    <  1:1075532/150‑1 (MQ=255)
                                            aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                    <  1:7021/150‑1 (MQ=255)
                                            aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCAGTTTATCTTTTTACACGCTAATTAATGCTGATTTTcg                                                                                                    >  2:1602215/1‑150 (MQ=255)
                                             gCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGAAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgc                                                                                                   <  1:932531/150‑1 (MQ=255)
                                              cAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATACGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcgcg                                                                                                  <  1:1248153/150‑1 (MQ=255)
                                                ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                                >  2:1723574/1‑150 (MQ=255)
                                                ggAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTAAACGCTAATTAATCCTCATTTTCGCGtt                                                                                                >  2:669118/1‑150 (MQ=255)
                                                 gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc                                                                                               <  1:506636/150‑1 (MQ=255)
                                                 gAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc                                                                                               <  2:1111572/150‑1 (MQ=255)
                                                  aaaaaGTCGCGCAATTTATGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGCATATTATCTACAGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCa                                                                                              >  2:587549/1‑150 (MQ=255)
                                                    acaGTCGCGCAATTTTTGTCAGGGGAAGTAATTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  2:1405999/148‑1 (MQ=255)
                                                    aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  1:1245183/150‑1 (MQ=255)
                                                    aaaGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGc                                                                                            <  1:1970897/150‑1 (MQ=255)
                                                      aGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCaa                                                                                          <  1:396394/150‑1 (MQ=255)
                                                       gTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAAt                                                                                         >  2:765366/1‑150 (MQ=255)
                                                            gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                    <  2:1002881/150‑1 (MQ=255)
                                                            gcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATATTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAAt                                                                                    >  1:336/1‑150 (MQ=255)
                                                                tttttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGAAGGATATTATCTCCTGCCGTTTATCATTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCCAg                                                                                >  2:168862/1‑150 (MQ=255)
                                                                   ttGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAgcgc                                                                             <  2:328048/150‑1 (MQ=255)
                                                                     gTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtt                                                                           >  1:1662144/1‑150 (MQ=255)
                                                                       cACGTGTAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttg                                                                         <  2:673315/150‑1 (MQ=255)
                                                                       cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtttt                                                                         >  1:1107878/1‑149 (MQ=255)
                                                                         cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCAGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                       >  2:1054659/1‑150 (MQ=255)
                                                                         cGTGAAGGAATTCGCAATTTCCTAGCCCGTCTCGATATTGAAGAATAAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                       <  1:130073/150‑1 (MQ=255)
                                                                            gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                    <  2:620912/150‑1 (MQ=255)
                                                                               ggAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTGTGTTGATACg                                                                 <  1:1262014/150‑1 (MQ=255)
                                                                                     cGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                           >  2:59458/1‑150 (MQ=255)
                                                                                      gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTCCAGCAATAAAATCCAGCGCTTTGGTGATACGCTCGATa                                                          >  2:1504475/1‑150 (MQ=255)
                                                                                      gCAATATCCTCGCCCGTCTCGATATTGACGAATCAGCATAAAAAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTCATTTTCGCGTTCAGCAATAAAATCCAGAGCTTTGTTGATACGCTCGATa                                                          >  1:43061/1‑150 (MQ=255)
                                                                                       cAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATaa                                                         >  2:1391966/1‑149 (MQ=255)
                                                                                       cAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATAc                                                         >  1:1141638/1‑150 (MQ=255)
                                                                                        aaTTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTGCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACt                                                        <  1:1662281/150‑1 (MQ=255)
                                                                                           ttCCTCGACAGTCTATATATTTACTAATCAGCATAAATAAAAAAAGGCAGGATATTATCTCCTGCAGTTTATCTTTTTACACGCTACTTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCg                                                     <  1:1415022/150‑1 (MQ=255)
                                                                                               tCGCCCGTCTCTATTTTGACGATTCAGCATTAATAAAAAACGGCAGGTTATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTTCTGCTGATTTTCGCGTTCAGCATTAATATCCTGCGCTTTGTTGATACGCTCGATACTGCGGGTc                                                 <  1:840565/150‑1 (MQ=255)
                                                                                               tCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTc                                                 <  2:585378/150‑1 (MQ=255)
                                                                                                 gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGAGTTCAGAAATAAAATCCATCGCTTTGTTGATACGCTCGATACTGAGGGTCtt                                               >  1:60591/1‑150 (MQ=255)
                                                                                                        tcGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATTATCTCCTGCAGTTTATCTTGTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAAtt                                        >  1:806918/1‑150 (MQ=255)
                                                                                                         cGATATTGACGAATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGGTCAGCAATAAAATCCAGCGCGTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                       <  1:1282843/150‑1 (MQ=255)
                                                                                                         cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                       <  1:1365368/150‑1 (MQ=255)
                                                                                                          gATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGc                                      >  2:463402/1‑150 (MQ=255)
                                                                                                           atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg                                     <  2:1018954/150‑1 (MQ=255)
                                                                                                             atTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                   >  1:619855/1‑150 (MQ=255)
                                                                                                               tGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGGTTATCTTTTTACACGCTAAGTACTGCTGAGTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTTCGGGTCTTACCAATTGCGTGaa                                 >  2:1498852/1‑150 (MQ=255)
                                                                                                                gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGCAc                                >  1:46908/1‑150 (MQ=255)
                                                                                                                gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAAc                                <  1:1164463/150‑1 (MQ=255)
                                                                                                                gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAATGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCTATACTGAGGGTATTACAAATTGCGTGaaa                                >  1:255502/1‑149 (MQ=255)
                                                                                                                 aCGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                               <  1:490544/150‑1 (MQ=255)
                                                                                                                 aCGAATCAGCATAAATAAAAAACGGCAGGATATTAGCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACg                               <  1:498138/150‑1 (MQ=255)
                                                                                                                    aaTCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTa                            >  2:965989/1‑150 (MQ=255)
                                                                                                                      tCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCAAATTACTGATGATTTTCGCGGTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTAGATACTGCGGGTCTAACCAATTGCGTGAACGGTAAc                          >  1:506420/1‑150 (MQ=255)
                                                                                                                        aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTCCCAATTGCGTGAACGGTAACAt                        >  2:1661576/1‑150 (MQ=255)
                                                                                                                        aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACAt                        >  2:1287616/1‑150 (MQ=255)
                                                                                                                         gCATAAATAAAAAACGGCAGGATATTTTCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc                       >  1:721418/1‑150 (MQ=255)
                                                                                                                             aaataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                   >  2:1235208/1‑150 (MQ=255)
                                                                                                                             aaaagataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGt                   >  2:864739/8‑150 (MQ=39)
                                                                                                                               ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc                 >  1:976736/1‑150 (MQ=255)
                                                                                                                               ataaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCCCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGc                 >  2:364945/1‑150 (MQ=255)
                                                                                                                                taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGGTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGcg                >  1:459355/1‑149 (MQ=255)
                                                                                                                                taaaAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCt                >  1:37540/1‑150 (MQ=255)
                                                                                                                                 aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGACCGGTAACATCCAGTGCTg               >  2:515271/1‑150 (MQ=255)
                                                                                                                                    aaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag            <  2:92169/150‑1 (MQ=255)
                                                                                                                                     aaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga           <  1:707630/150‑1 (MQ=255)
                                                                                                                                     aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACCCGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga           >  1:570941/1‑150 (MQ=255)
                                                                                                                                     aaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgaga           >  2:1779106/1‑150 (MQ=255)
                                                                                                                                        ggCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCAAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTg        >  1:1267253/1‑150 (MQ=255)
                                                                                                                                          cAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcc      >  2:1167237/1‑150 (MQ=255)
                                                                                                                                            ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcccc    <  1:1111174/150‑1 (MQ=255)
                                                                                                                                              atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGc  >  2:1668201/1‑150 (MQ=255)
                                                                                                                                              atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGc  <  1:464860/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGC  >  NC_000913/2519079‑2519370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑