Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,654,142 T→A 6.3% noncoding (288/304 nt) ryfA → novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,654,1420TA 6.3% 357.8 / 11.0 136noncoding (288/304 nt)ryfAnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (78/49);  new base A (3/6);  total (81/55)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 137 total aligned reads displayed.

ACCGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653993‑2654290
                                                                                                                                                     |                                                                                                                                                    
aCCGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCt                                                                                                                                                      >  1:664336/1‑150 (MQ=255)
       aaTGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGggcg                                                                                                                                               >  2:1118286/1‑150 (MQ=255)
           aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  2:846029/150‑1 (MQ=255)
           aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                           <  2:1461410/150‑1 (MQ=255)
            tgATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCatt                                                                                                                                          <  2:729919/149‑2 (MQ=255)
               aaCGGTGCGTTTGTCTCGTCCTTATTCTTTCTGGTGCGCTGTTAACCGAGGAAAATAATACCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCattcgg                                                                                                                                       <  1:2069914/149‑5 (MQ=255)
                aCGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATAtttt                                                                                                                                      >  2:1003803/1‑150 (MQ=255)
                 cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                     >  2:1389215/1‑150 (MQ=255)
                 cGGTGCTTTTGAAACGTACTTATTGTATCTGGTGCGCTGTTAACCGAGGTAAATAATAACCTGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCATAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                     <  1:377199/150‑1 (MQ=255)
                  ggTTCTTTTGTATCGTACTTATTGGTTCTCGTGCGCTGTTATCCGATGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGCGGTTAACAACCTTCAGAGCAGCAAGTAAGCCAGAATTCCGCCCAAATGGCGGCattctggct                                                                                                                                    <  2:1237181/150‑8 (MQ=255)
                  ggTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAg                                                                                                                                    <  1:1228762/150‑1 (MQ=255)
                  ggTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAg                                                                                                                                    >  1:230704/1‑150 (MQ=255)
                     gattttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAtt                                                                                                                                 <  1:93945/148‑1 (MQ=255)
                      cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  2:1662357/1‑150 (MQ=255)
                      cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  2:726460/1‑150 (MQ=255)
                      cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                >  1:1903092/1‑150 (MQ=255)
                         ttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATcc                                                                                                                             <  2:950433/150‑1 (MQ=255)
                          tGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTTGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCTCCCTTTGGGCGGCATATTTTATATTATCCg                                                                                                                            >  1:2041491/1‑150 (MQ=255)
                             aTCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGAtt                                                                                                                         <  1:77994/150‑1 (MQ=255)
                                       ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                               <  2:1849144/150‑1 (MQ=255)
                                       ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAAAAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                               <  2:1412741/150‑1 (MQ=255)
                                        tGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCTTATTTTAGATTATCCGATTCTGTTTAAAgg                                                                                                              >  2:1733207/1‑149 (MQ=255)
                                         gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  1:863812/1‑150 (MQ=255)
                                         gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  2:2087439/1‑150 (MQ=255)
                                         gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                             >  2:2095155/1‑150 (MQ=255)
                                          tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                            <  2:609890/150‑1 (MQ=255)
                                            tCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACg                                                                                                          >  1:856932/1‑150 (MQ=255)
                                                  gcgcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGCGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGATTAAAGTCACGCaaaaa                                                                                                    >  2:442663/1‑150 (MQ=255)
                                                   cgcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaaaa                                                                                                   >  2:1491920/1‑150 (MQ=255)
                                                     cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  2:233782/150‑1 (MQ=255)
                                                     cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 >  2:570438/1‑150 (MQ=255)
                                                     cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 >  2:1825620/1‑150 (MQ=255)
                                                     cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  1:404200/150‑1 (MQ=255)
                                                     cTGTTAACCGAGGTAAATAATAACCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                 <  2:189534/150‑1 (MQ=255)
                                                       gTTAACCGATGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAaccac                                                                                               <  2:1523881/150‑1 (MQ=255)
                                                       gTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAaccac                                                                                               >  2:161978/1‑150 (MQ=255)
                                                         tAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGCGCAGAAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAGTATACGATTCTGTTTAAATTCACGCATAAAACCAccc                                                                                             >  1:384618/1‑150 (MQ=255)
                                                         tAACCGAGCTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGACCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAATCCAccc                                                                                             >  1:390514/1‑150 (MQ=255)
                                                           aCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGATAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCa                                                                                           >  2:1181799/1‑150 (MQ=255)
                                                             cGAGGTAAATAATAACCGTAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGc                                                                                         <  1:1141737/150‑1 (MQ=255)
                                                              gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGTTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                        <  1:206632/150‑1 (MQ=255)
                                                               aGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGa                                                                                       <  1:67726/150‑1 (MQ=255)
                                                                 gTAAATAATAACCGGATTCTATCCGTCGACAATTTACTGGTGGTTAACAACCTTCAGATCAGCAAGTAAGCCCGAAAGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACg                                                                                     <  1:82826/150‑1 (MQ=255)
                                                                  tAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATTTTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGt                                                                                    >  2:1640952/1‑150 (MQ=255)
                                                                    aataatAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATACGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGAAGtta                                                                                  >  2:597393/1‑149 (MQ=255)
                                                                     ataataACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTGTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCa                                                                                 >  2:1894556/1‑150 (MQ=255)
                                                                      taataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCAt                                                                                >  1:708360/1‑150 (MQ=255)
                                                                       aataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATa                                                                               <  1:1389215/150‑1 (MQ=255)
                                                                       aataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATa                                                                               >  1:1818205/1‑150 (MQ=255)
                                                                         taaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGGATATTTTAGCTTAGCCGATTCTGTTTAAAGTGACGCAAATCACCCCCCCAGCGTCGTTCATAgc                                                                             >  1:411556/1‑149 (MQ=255)
                                                                             cGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATgg                                                                         <  2:664336/150‑1 (MQ=255)
                                                                             cGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCAAATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATgg                                                                         <  1:1281810/150‑1 (MQ=255)
                                                                              ggAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAACACCACCCCAGCGACGTTCATAGAATGgc                                                                        >  1:250938/1‑150 (MQ=255)
                                                                               gAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGgcg                                                                       <  1:1963739/150‑1 (MQ=255)
                                                                                aGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGgcgc                                                                      <  1:72696/150‑1 (MQ=255)
                                                                                   ctctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGAGCAat                                                                   >  1:1014680/1‑150 (MQ=255)
                                                                                    tctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAata                                                                  <  2:1576710/150‑1 (MQ=255)
                                                                                     ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTTGGCGGCATATTTTAGATTATCCGATTATGTTTAAAGTCACGCAAAACACGACACCAGCGACGTTAATAGAATTGGGCAatat                                                                 >  1:1836286/1‑150 (MQ=255)
                                                                                     ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAatat                                                                 >  2:896474/1‑150 (MQ=255)
                                                                                       ccGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGt                                                               >  2:512354/1‑150 (MQ=255)
                                                                                           cGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCgg                                                           >  1:1156377/1‑150 (MQ=255)
                                                                                             aCAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGta                                                         <  2:230704/150‑1 (MQ=255)
                                                                                               aaTTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGCGCAGCTTATTTTAGATTATACGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGAGAACTTAATATAATGGCGCAATATGTTCGGtaat                                                       >  1:1563122/1‑150 (MQ=255)
                                                                                                       ggtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGc                                               <  2:1608954/150‑1 (MQ=255)
                                                                                                       ggtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCAGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGAAGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGc                                               >  2:659755/1‑150 (MQ=255)
                                                                                                        gtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCt                                              <  2:454171/150‑1 (MQ=255)
                                                                                                         tggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAACCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTa                                             >  1:1295019/1‑150 (MQ=255)
                                                                                                          ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                            >  2:23391/1‑150 (MQ=255)
                                                                                                          ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGAATCTGTGTAAAGTCCCGAAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGGATGAAAGTGGCTaa                                            >  2:749697/1‑150 (MQ=255)
                                                                                                              aacaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTcc                                        <  1:257361/150‑1 (MQ=255)
                                                                                                                 aacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGGGGGATATTTTAGATTATCCGATTCAGTTTAAATTCACCCAAAAAACCACCCCCGCGACGTTCATAGAATGGGGCAATATGTTAGGTAATAAAGTGGCTATATCCttt                                     >  1:1855468/1‑150 (MQ=255)
                                                                                                                   ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATTGAGCAATATGTTGGGTAATAAAGTGGCTAATTCCttttt                                   >  1:1921097/1‑150 (MQ=255)
                                                                                                                   ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGATTAAAGTCACGCAAAAAAACACCCCAGCGACGTTCATAGAATGGCGCAATATGTTAGGTAATAAAGTGGCGAATTACttttt                                   >  2:1590146/1‑150 (MQ=255)
                                                                                                                    cTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTc                                  >  1:1378147/1‑150 (MQ=255)
                                                                                                                       cAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCCCGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTcccc                               >  1:726042/1‑150 (MQ=255)
                                                                                                                         gagCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtt                             >  1:762850/1‑150 (MQ=255)
                                                                                                                          agcagcAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCCATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtgt                            >  2:1734325/1‑148 (MQ=255)
                                                                                                                           gcagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtttt                           >  1:1132600/1‑150 (MQ=255)
                                                                                                                           gcagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGACACGCAAAAAACCACCCCAGCGACGTTCATATAATGGCGCAATATGTTCGGTAAATAAGAGGCTAATTCCATTTTCCCCtttt                           >  2:781187/1‑150 (MQ=255)
                                                                                                                            cagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCttttt                          <  1:2087439/150‑1 (MQ=255)
                                                                                                                             agcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtttttt                         >  1:1211051/1‑150 (MQ=255)
                                                                                                                                aaGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTTGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCCGCGACGTTCATAGCATGTGCCAATATGTTCGGTAATAAAGTGGCTAATTACTTTTTGCCCTTTTTTCAc                      >  2:1513218/1‑150 (MQ=255)
                                                                                                                                 aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                     >  1:567734/1‑150 (MQ=255)
                                                                                                                                  gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCt                    <  1:1126042/150‑1 (MQ=255)
                                                                                                                                   tAAGCCCGAATGGCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGATTAAAGTCACGCAATACACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGATATAACGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTg                   <  1:1758849/150‑1 (MQ=255)
                                                                                                                                   tAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTg                   <  2:359670/150‑1 (MQ=255)
                                                                                                                                   tAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTg                   <  1:1854724/150‑1 (MQ=255)
                                                                                                                                     ctaaaataTGCCGCCCAAAGGGCGGCATATTTTAGATAATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCCGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCATTTTTCACCAGAc                 >  2:1563062/8‑150 (MQ=255)
                                                                                                                                     aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTTTCCGATTCTGTTTAAAGTCCCGCAAAAAACCACCCCAGCGACGTTGATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTACCCTTTTTTAACCTGAc                 >  1:1866289/1‑150 (MQ=255)
                                                                                                                                     aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGag                 <  1:1491920/150‑2 (MQ=255)
                                                                                                                                        ccGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACaaa              <  1:938816/150‑1 (MQ=255)
                                                                                                                                          gAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  2:411862/1‑150 (MQ=255)
                                                                                                                                          ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata            >  1:13992/3‑150 (MQ=255)
                                                                                                                                            aTGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATc          <  2:1903092/150‑1 (MQ=255)
                                                                                                                                               ccgccCTTTGGGCGGCATATTTTAGATTATCAGATTCTGTTTAAAGTCACGAAAAAAACCACCCCAGAGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTAAATTTTCCCCTTTTTTCAACTGACAAATATCGAt       >  1:1408569/1‑150 (MQ=255)
                                                                                                                                                  cccTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGGAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTgg    >  1:141190/1‑150 (MQ=255)
                                                                                                                                                   ccTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGt   >  1:1529852/1‑150 (MQ=255)
                                                                                                                                                   ccTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGt   <  2:2069631/150‑1 (MQ=255)
                                                                                                                                                    cTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGtt  <  2:1446580/150‑1 (MQ=255)
                                                                                                                                                    cTTTGGGCGGCATATTTTACATTATCAGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATAACGATAggat  >  2:776263/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCGCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTT  >  NC_000913/2653993‑2654290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: