Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,654,143 T→A 6.2% noncoding (289/304 nt) ryfA → novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,654,1430TA 6.2% 388.0 / 8.0 136noncoding (289/304 nt)ryfAnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (77/49);  new base A (2/6);  total (81/55)
Fisher's exact test for biased strand distribution p-value = 6.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 138 total aligned reads displayed.

GCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCA  >  NC_000913/2653996‑2654292
                                                                                                                                                   |                                                                                                                                                     
gCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGGGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTg                                                                                                                                                     <  1:1349275/150‑1 (MQ=255)
       gAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCa                                                                                                                                              <  1:1928322/150‑1 (MQ=255)
        aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                             <  2:846029/150‑1 (MQ=255)
        aaGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCat                                                                                                                                             <  2:1461410/150‑1 (MQ=255)
         tgATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCatt                                                                                                                                            <  2:729919/149‑2 (MQ=255)
            tACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATAttt                                                                                                                                         <  2:1656745/150‑1 (MQ=255)
            aaCGGTGCGTTTGTCTCGTCCTTATTCTTTCTGGTGCGCTGTTAACCGAGGAAAATAATACCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCAAAGGGCGGCattcgg                                                                                                                                         <  1:2069914/149‑5 (MQ=255)
             aCGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATAtttt                                                                                                                                        >  2:1003803/1‑150 (MQ=255)
              cGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTa                                                                                                                                       >  2:1605525/1‑150 (MQ=255)
               ggTTCTTTTGTATCGTACTTATTGGTTCTCGTGCGCTGTTATCCGATGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGCGGTTAACAACCTTCAGAGCAGCAAGTAAGCCAGAATTCCGCCCAAATGGCGGCattctggct                                                                                                                                      <  2:1237181/150‑8 (MQ=255)
               ggTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAg                                                                                                                                      <  1:1228762/150‑1 (MQ=255)
                  gattttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAtt                                                                                                                                   <  1:93945/148‑1 (MQ=255)
                   cTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTa                                                                                                                                  >  1:1903092/1‑150 (MQ=255)
                      ttGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATcc                                                                                                                               <  2:950433/150‑1 (MQ=255)
                       tGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTTGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCTCCCTTTGGGCGGCATATTTTATATTATCCg                                                                                                                              >  1:2041491/1‑150 (MQ=255)
                          aTCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGAtt                                                                                                                           <  1:77994/150‑1 (MQ=255)
                           tCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTc                                                                                                                          <  2:1499421/150‑1 (MQ=255)
                               aCTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGtt                                                                                                                      >  1:896791/1‑150 (MQ=255)
                               aCTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCATTGGGCGGCATATTTTAGATTATCCGATTCTGtt                                                                                                                      >  2:1427396/1‑150 (MQ=255)
                                    ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                                 <  2:1849144/150‑1 (MQ=255)
                                    ttGTTTCTGGTGCGCTGTTAACCGAGGTAAATAAAAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAg                                                                                                                 <  2:1412741/150‑1 (MQ=255)
                                     tGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCTTATTTTAGATTATCCGATTCTGTTTAAAgg                                                                                                                >  2:1733207/1‑149 (MQ=255)
                                      gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                               >  1:1424650/1‑150 (MQ=255)
                                      gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                               >  2:2095155/1‑150 (MQ=255)
                                      gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                               >  2:2087439/1‑150 (MQ=255)
                                      gTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTc                                                                                                               >  1:863812/1‑150 (MQ=255)
                                       tttCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCa                                                                                                              <  2:609890/150‑1 (MQ=255)
                                                cgcTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCaaaaaa                                                                                                     >  2:1491920/1‑150 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                   <  1:404200/150‑1 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                   >  1:454171/1‑150 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                   <  2:233782/150‑1 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                   >  2:570438/1‑150 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGAAAGTAAGCCCGAATGCCGCCCTTTGTGCGGCATATTTTAGATTATAAGATTCTGTTTAAAGTCACCAAAAAAacc                                                                                                   >  2:1331324/1‑150 (MQ=255)
                                                  cTGTTAACCGAGGTAAATAATAACCGGAGGCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAacc                                                                                                   <  2:189534/150‑1 (MQ=255)
                                                    gTTAACCGATGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAaccac                                                                                                 <  2:1523881/150‑1 (MQ=255)
                                                    gTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAaccac                                                                                                 >  2:161978/1‑150 (MQ=255)
                                                      tAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGCGCAGAAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGAGTATACGATTCTGTTTAAATTCACGCATAAAACCAccc                                                                                               >  1:384618/1‑150 (MQ=255)
                                                      tAACCGAGCTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGACCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAATCCAccc                                                                                               >  1:390514/1‑150 (MQ=255)
                                                        aCCGAGGTAAATAATAACCGGAGTCTCTCCGGCGATAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCa                                                                                             >  2:1181799/1‑150 (MQ=255)
                                                         ccGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAg                                                                                            >  1:690272/1‑150 (MQ=255)
                                                         ccGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACAAcccacg                                                                                            >  1:1819239/1‑147 (MQ=255)
                                                          cGAGGTAAATAATAACCGTAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGc                                                                                           <  1:1141737/150‑1 (MQ=255)
                                                           gAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGTTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCg                                                                                          <  1:206632/150‑1 (MQ=255)
                                                            aGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGa                                                                                         <  1:67726/150‑1 (MQ=255)
                                                              gTAAATAATAACCGGATTCTATCCGTCGACAATTTACTGGTGGTTAACAACCTTCAGATCAGCAAGTAAGCCCGAAAGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACg                                                                                       <  1:82826/150‑1 (MQ=255)
                                                               tAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATTTTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGt                                                                                      >  2:1640952/1‑150 (MQ=255)
                                                                 aataatAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATACGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGAAGtta                                                                                    >  2:597393/1‑149 (MQ=255)
                                                                   taataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCTAGTAAGCCCGAATGCCGCCCTGTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAATCCCACCACAGCGAGGTTCAt                                                                                  >  1:166355/1‑150 (MQ=255)
                                                                   taataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCAt                                                                                  >  1:708360/1‑150 (MQ=255)
                                                                    aataaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATa                                                                                 >  1:1818205/1‑150 (MQ=255)
                                                                      taaCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGGATATTTTAGCTTAGCCGATTCTGTTTAAAGTGACGCAAATCACCCCCCCAGCGTCGTTCATAgc                                                                               >  1:411556/1‑149 (MQ=255)
                                                                        aCCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAAt                                                                             >  2:1854724/1‑150 (MQ=255)
                                                                          cGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATgg                                                                           <  2:664336/150‑1 (MQ=255)
                                                                           ggAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAACACCACCCCAGCGACGTTCATAGAATGgc                                                                          >  1:250938/1‑150 (MQ=255)
                                                                             aGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGgcgc                                                                        <  1:72696/150‑1 (MQ=255)
                                                                                ctctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGAGCAat                                                                     >  1:1014680/1‑150 (MQ=255)
                                                                                  ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTTGGCGGCATATTTTAGATTATCCGATTATGTTTAAAGTCACGCAAAACACGACACCAGCGACGTTAATAGAATTGGGCAatat                                                                   >  1:1836286/1‑150 (MQ=255)
                                                                                  ctcCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAatat                                                                   >  2:896474/1‑150 (MQ=255)
                                                                                    ccGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGt                                                                 >  2:512354/1‑150 (MQ=255)
                                                                                        cGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCgg                                                             >  1:1156377/1‑150 (MQ=255)
                                                                                          aCAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTTAAGTCACGCAAAAAACCACCCCAGCGACGGTCATAGAATGGCGGAATATGTTCGGta                                                           >  1:735315/1‑150 (MQ=255)
                                                                                          aCAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGta                                                           <  2:230704/150‑1 (MQ=255)
                                                                                            aaTTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGCGCAGCTTATTTTAGATTATACGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGAGAACTTAATATAATGGCGCAATATGTTCGGtaat                                                         >  1:1563122/1‑150 (MQ=255)
                                                                                                tACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATaaat                                                     >  2:412186/1‑149 (MQ=255)
                                                                                                  cTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTg                                                   <  1:1397678/150‑1 (MQ=255)
                                                                                                  cTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTg                                                   >  2:1820915/1‑150 (MQ=255)
                                                                                                    ggtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATTAAGTggg                                                 >  2:668659/1‑149 (MQ=255)
                                                                                                    ggtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCAGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGAAGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGc                                                 >  2:659755/1‑150 (MQ=255)
                                                                                                     gtggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCt                                                <  2:454171/150‑1 (MQ=255)
                                                                                                      tggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAACCCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTa                                               >  1:1295019/1‑150 (MQ=255)
                                                                                                       ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTaa                                              >  2:23391/1‑150 (MQ=255)
                                                                                                       ggtTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGAATCTGTGTAAAGTCCCGAAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGGATGAAAGTGGCTaa                                              >  2:749697/1‑150 (MQ=255)
                                                                                                         ttAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAAtt                                            >  1:292879/1‑150 (MQ=255)
                                                                                                           aacaacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTcc                                          <  1:257361/150‑1 (MQ=255)
                                                                                                             caacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAACACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCtt                                        >  1:1864565/1‑150 (MQ=255)
                                                                                                              aacCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGGGGGATATTTTAGATTATCCGATTCAGTTTAAATTCACCCAAAAAACCACCCCCGCGACGTTCATAGAATGGGGCAATATGTTAGGTAATAAAGTGGCTATATCCttt                                       >  1:1855468/1‑150 (MQ=255)
                                                                                                                ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATTGAGCAATATGTTGGGTAATAAAGTGGCTAATTCCttttt                                     >  1:1921097/1‑150 (MQ=255)
                                                                                                                ccTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGATTAAAGTCACGCAAAAAAACACCCCAGCGACGTTCATAGAATGGCGCAATATGTTAGGTAATAAAGTGGCGAATTACttttt                                     >  2:1590146/1‑150 (MQ=255)
                                                                                                                 cTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTc                                    >  1:1378147/1‑150 (MQ=255)
                                                                                                                    cAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTcccc                                 <  2:1093112/150‑1 (MQ=255)
                                                                                                                       agcagcAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCCATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtgt                              >  2:1734325/1‑148 (MQ=255)
                                                                                                                        gcagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCCCCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtttt                             >  1:1132600/1‑150 (MQ=255)
                                                                                                                        gcagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGACACGCAAAAAACCACCCCAGCGACGTTCATATAATGGCGCAATATGTTCGGTAAATAAGAGGCTAATTCCATTTTCCCCtttt                             >  2:781187/1‑150 (MQ=255)
                                                                                                                         cagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCttttt                            <  1:2095155/150‑1 (MQ=255)
                                                                                                                         cagcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCttttt                            <  1:2087439/150‑1 (MQ=255)
                                                                                                                          agcaAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCtttttt                           >  1:1211051/1‑150 (MQ=255)
                                                                                                                              aGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTAAAAGTCACGCAAACAACCACCCCAGCGACGTTCATAGCATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                       >  2:1941237/1‑150 (MQ=255)
                                                                                                                              aGTAAGCACGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCAcc                       <  2:1563122/150‑1 (MQ=255)
                                                                                                                               gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCt                      >  1:780415/1‑150 (MQ=255)
                                                                                                                               gTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCt                      <  1:1126042/150‑1 (MQ=255)
                                                                                                                                tAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTg                     <  2:359670/150‑1 (MQ=255)
                                                                                                                                  ctaaaataTGCCGCCCAAAGGGCGGCATATTTTAGATAATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCCGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCATTTTTCACCAGAc                   >  2:1563062/8‑150 (MQ=255)
                                                                                                                                  aGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGag                   <  1:1491920/150‑2 (MQ=255)
                                                                                                                                       gAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata              >  2:411862/1‑150 (MQ=255)
                                                                                                                                       ataTGCCGCCCAAAGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAata              >  1:13992/3‑150 (MQ=255)
                                                                                                                                         aTGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATc            <  2:1903092/150‑1 (MQ=255)
                                                                                                                                            ccgccCTTTGGGCGGCATATTTTAGATTATCAGATTCTGTTTAAAGTCACGAAAAAAACCACCCCAGAGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTAAATTTTCCCCTTTTTTCAACTGACAAATATCGAt         >  1:1408569/1‑150 (MQ=255)
                                                                                                                                                 cTTTGGGCGGCATATTTTACATTATCAGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATAACGATAggat    >  2:776263/1‑148 (MQ=255)
                                                                                                                                                   ttGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCa  <  1:1686015/150‑1 (MQ=255)
                                                                                                                                                   ttGGGAGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCAAGCAAAAAACAACCGCAGCGACGTTCATAGAATGTCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTATTTCACCTGACAAATATCGATTGGTTCa  <  1:123505/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCACAATGAAGATACGGTGCTTTTGTATCGTACTTATTGTTTCTGGTGCGCTGTTAACCGAGGTAAATAATAACCGGAGTCTCTCCGGCGACAATTTACTGGTGGTTAACAACCTTCAGAGCAGCAAGTAAGCCCGAATGCCGCCCTTTGGGCGGCATATTTTAGATTATCCGATTCTGTTTAAAGTCACGCAAAAAACCACCCCAGCGACGTTCATAGAATGGCGCAATATGTTCGGTAATAAAGTGGCTAATTCCTTTTTCCCCTTTTTTCACCTGACAAATATCGATTGGTTCA  >  NC_000913/2653996‑2654292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: