Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,768,620 G→A 5.0% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,768,6200GA 5.0% 519.8 / 9.6 158intergenic (+47/‑45)yfjP/yfjQCP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (67/83);  new base A (4/4);  total (71/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-01

Only 100 of 161 total aligned reads displayed.

GCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAG  >  NC_000913/2768473‑2768769
                                                                                                                                                   |                                                                                                                                                     
gCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGGTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCa                                                                                                                                                     <  2:970342/150‑1 (MQ=255)
 cACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGTCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                                    <  2:1842461/150‑1 (MQ=255)
    cTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCATTGGACCGCGATGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAGGGc                                                                                                                                                 <  2:1303474/150‑1 (MQ=25)
    cTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGc                                                                                                                                                 <  1:1081419/150‑1 (MQ=255)
    cTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGc                                                                                                                                                 <  2:1632417/150‑1 (MQ=255)
    cTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGc                                                                                                                                                 <  1:849065/150‑1 (MQ=255)
     tttCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGGGGCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAGGGct                                                                                                                                                <  2:24114/150‑1 (MQ=25)
         cccTTATTCCCGCCCCGGTGCGGACCATTTTTAAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCGTCTGCTGAGTGTTTAATTCTGCGCGATTATTCTCCGAGCCCTGTCGGCAGTGCGGCAGGGctttct                                                                                                                                            >  1:348511/1‑150 (MQ=25)
         cccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCTGGGctttct                                                                                                                                            >  1:75761/1‑150 (MQ=255)
           cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctgt                                                                                                                                          >  2:1134519/1‑148 (MQ=255)
            ttATTCCCGCCCCGTTGCGGACCATTATTCCGGCCGTGCGTTCCTCGGTGGGGTCAGTGGCCCGCGCTGTCTGGTATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTa                                                                                                                                         <  2:1900054/150‑1 (MQ=255)
            ttATTCCCGCCCAGTTGCGGACAATTATTGACGCCGTGCGTGCCTCGGTGGTGTCAGTGGCCATCGCTGTATGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTTTTATTCTCCGAGACCTGTCGCAAGGGCGGCAGGGCTTTCTTTa                                                                                                                                         <  1:1111939/150‑1 (MQ=37)
              aTTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAtt                                                                                                                                       <  2:1586770/150‑1 (MQ=255)
                tCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTg                                                                                                                                     <  2:1638429/150‑1 (MQ=255)
                tCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGAGTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTg                                                                                                                                     <  1:770465/150‑1 (MQ=255)
                tCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGGTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTg                                                                                                                                     >  1:922750/1‑150 (MQ=255)
                tCCCGCCCCGGGGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTg                                                                                                                                     <  2:36075/150‑1 (MQ=255)
                  ccGCCCCGGTGCGGACCATTATTCCGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGtt                                                                                                                                   >  2:1537949/1‑150 (MQ=255)
                  ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCATCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGtt                                                                                                                                   <  1:436977/150‑1 (MQ=255)
                     ccccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTa                                                                                                                                <  1:1154752/150‑1 (MQ=255)
                      cccGGGGCGGACCTTTATTCAGGCCGTGCGTTCCTGTGTGGGTTCAGTGGCCCGGGCTGTCGGGGATTTATTCATCTTAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGGGGCAGGGCTTTATTTATTTGTTTTAc                                                                                                                               <  2:1734181/150‑1 (MQ=255)
                       ccGGTGCGGACCATTATTCAGGCCGTGGGTTCCTCGGTTGTGTCAGTGGCCCGCGCTGTCTGGGAGTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                              <  2:1507654/150‑1 (MQ=255)
                       ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                              >  2:1739501/1‑150 (MQ=255)
                       ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTAGTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                              >  1:383577/1‑150 (MQ=255)
                              ggACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTg                                                                                                                       >  1:717421/1‑150 (MQ=255)
                                aCCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgag                                                                                                                     >  1:2047828/1‑150 (MQ=255)
                                 ccATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCTGGGCTTTCTTTATTTGTTTTTCCCATACTgagg                                                                                                                    >  2:1922307/1‑150 (MQ=255)
                                 acATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgagg                                                                                                                    <  2:231158/149‑1 (MQ=255)
                                   attattCAGTCCGTGCGTTCTTCGGTGGTGTCCTTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTATAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgaggag                                                                                                                  <  2:1386903/150‑1 (MQ=39)
                                         cAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCt                                                                                                            <  2:1940898/150‑1 (MQ=255)
                                         cAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCt                                                                                                            <  1:1295983/150‑1 (MQ=255)
                                           ggCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTa                                                                                                          >  2:464932/1‑150 (MQ=255)
                                           ggCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGATTa                                                                                                          >  2:690470/1‑150 (MQ=255)
                                            gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTAt                                                                                                         >  1:1503917/1‑150 (MQ=255)
                                             ccGTGCGTTCCTCGGTGGTGTCAGTGTCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATg                                                                                                        <  1:158324/150‑1 (MQ=255)
                                                tGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTTGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAcc                                                                                                     <  2:1323581/150‑1 (MQ=255)
                                                 gCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAccc                                                                                                    >  1:1343267/1‑150 (MQ=255)
                                                   gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                                  >  2:564701/1‑150 (MQ=255)
                                                      ccTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTg                                                                                               <  2:1458934/150‑1 (MQ=255)
                                                      ccTCGGTGGGGTCAGTGTCCAGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGATCCCTGTCGCAAGTGCGGCTGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGGCTGCTTATGACCCGTCTg                                                                                               <  1:577901/150‑1 (MQ=38)
                                                        tCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCGTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGc                                                                                             >  2:294511/1‑150 (MQ=255)
                                                          ggtggtGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCtt                                                                                           <  2:1849591/150‑1 (MQ=255)
                                                              gtgtCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTcgcg                                                                                       >  1:700425/1‑150 (MQ=255)
                                                                   aGTGGCCCGCGCTGTCTGGGTTTTCTTCGTCTGAGTGTTTACTTCTGGCCTATTATTCTCCGAGCCCTGTCGGAAGTGCGGCAGGGCTTTCTTTATTTGATTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                                  <  2:205197/150‑1 (MQ=38)
                                                                   aGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGCGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                                  <  2:27086/150‑1 (MQ=255)
                                                                   aGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTg                                                                                  <  2:2080752/150‑1 (MQ=255)
                                                                    gTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTgg                                                                                 >  2:1322158/1‑150 (MQ=255)
                                                                     tGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCAGGCTTAGCGCTTTggg                                                                                >  2:1994047/1‑149 (MQ=255)
                                                                      ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                               <  1:684895/150‑1 (MQ=255)
                                                                          cgcgCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGc                                                                           >  1:1634185/1‑150 (MQ=255)
                                                                          cgcgCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGc                                                                           <  2:2102973/150‑1 (MQ=255)
                                                                           gcgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCa                                                                          <  2:350008/150‑1 (MQ=255)
                                                                             gcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCaaa                                                                        <  1:836332/150‑1 (MQ=255)
                                                                                gTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCt                                                                     >  2:1755268/1‑150 (MQ=255)
                                                                                  cTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTa                                                                   >  2:652474/1‑150 (MQ=255)
                                                                                         ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                            <  2:1917211/150‑1 (MQ=255)
                                                                                         ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGACg                                                            >  1:2074244/1‑150 (MQ=255)
                                                                                          tcttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGt                                                           <  2:407070/150‑1 (MQ=255)
                                                                                          tcttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGt                                                           <  1:1404639/150‑1 (MQ=255)
                                                                                            ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCTCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                         <  1:1626882/150‑1 (MQ=255)
                                                                                             tcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAc                                                        <  2:116715/150‑1 (MQ=255)
                                                                                             tcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAGTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAc                                                        <  1:59646/150‑1 (MQ=255)
                                                                                              cttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAcc                                                       >  2:1259243/1‑150 (MQ=255)
                                                                                                tctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTTCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCAGTGAccgt                                                     >  1:921794/1‑150 (MQ=255)
                                                                                                tctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgt                                                     >  2:1591869/1‑150 (MQ=255)
                                                                                                tctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgt                                                     >  1:872797/1‑150 (MQ=255)
                                                                                                 ctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtc                                                    >  1:1125519/1‑150 (MQ=255)
                                                                                                 ctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtc                                                    <  1:1186811/150‑1 (MQ=255)
                                                                                                  tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                                   <  2:587892/150‑1 (MQ=255)
                                                                                                  tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                                   <  2:1724155/150‑1 (MQ=255)
                                                                                                  tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                                   >  1:255643/1‑150 (MQ=255)
                                                                                                   gAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtccg                                                  <  1:1924484/150‑1 (MQ=255)
                                                                                                   gAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtccg                                                  >  1:1938550/1‑150 (MQ=255)
                                                                                                      tgtTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTa                                               >  1:268581/1‑150 (MQ=255)
                                                                                                       gtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTaa                                              >  2:776889/1‑150 (MQ=255)
                                                                                                       gtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTaa                                              <  1:1398189/150‑1 (MQ=255)
                                                                                                       gtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTaa                                              >  2:1201685/1‑150 (MQ=255)
                                                                                                       gtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTATGGCGCTGCAAATCATATTCGCCGTGACCGTCCGTTaa                                              >  2:1698460/1‑150 (MQ=255)
                                                                                                          tAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGGTAAccc                                           >  1:2085536/1‑150 (MQ=255)
                                                                                                             ttCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTg                                        <  2:1529524/150‑1 (MQ=255)
                                                                                                              tCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGa                                       <  2:1014445/150‑1 (MQ=255)
                                                                                                                  cccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTGAATTTGTTTTACCCATACTGAGGAGGCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAagag                                   <  1:1277376/150‑1 (MQ=255)
                                                                                                                      attattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAt                               <  2:1777094/150‑1 (MQ=255)
                                                                                                                       ttattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAtt                              >  2:609892/1‑150 (MQ=255)
                                                                                                                       ttattCTCCGAGACCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAtt                              <  1:1376402/150‑1 (MQ=255)
                                                                                                                           tctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGCCCGTCCGTTAACCCGTGAAGAGTTATTTcgc                          >  2:650414/1‑150 (MQ=255)
                                                                                                                              ccGAGCCCTGTCGCAAGGGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGGCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtg                       <  2:508621/150‑1 (MQ=255)
                                                                                                                                 aGCCGTGTCGCAAGTGCGGCAGGGCTTTCTTTATATGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGATTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggtg                    <  2:131118/150‑1 (MQ=255)
                                                                                                                                  gCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGCCCCGTCTGGCTTCGCGCTTTGGCGCTGCATATCTTATTCGCAGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGc                   >  1:1914732/1‑150 (MQ=21)
                                                                                                                                  gCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGATTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGc                   >  1:122903/1‑150 (MQ=21)
                                                                                                                                    ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCAAACTGAGGAGTCTGCTTATGACCCGTGTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGGCCGTCCGGTAACCCGGGACGAGTTATTTCGCGTAGTGccc                 >  2:1355613/1‑150 (MQ=25)
                                                                                                                                      tGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAg               <  2:1825300/150‑1 (MQ=255)
                                                                                                                                        gcGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGTGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCg             <  2:75761/149‑1 (MQ=255)
                                                                                                                                         cGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGt            >  2:436477/1‑150 (MQ=255)
                                                                                                                                             aGTGCGGCAGGGCTGTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTc        <  2:1057580/150‑1 (MQ=255)
                                                                                                                                              gTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAACTCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTcc       >  1:1314579/1‑149 (MQ=255)
                                                                                                                                                gCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGt     <  2:506578/150‑1 (MQ=255)
                                                                                                                                                  ggCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTTACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGa   >  1:158217/1‑150 (MQ=255)
                                                                                                                                                   gCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAg  >  1:745232/1‑150 (MQ=255)
                                                                                                                                                   gCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAg  <  1:1205564/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAG  >  NC_000913/2768473‑2768769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: