Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,788,676 A→T 5.4% intergenic (+27/‑309) ygaQ → / → csiD uncharacterized protein; putative enzyme/carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,788,6760AT 5.4% 374.0 / 4.5 132intergenic (+27/‑309)ygaQ/csiDuncharacterized protein; putative enzyme/carbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (62/61);  new base T (2/7);  total (64/68)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

AAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788528‑2788822
                                                                                                                                                    |                                                                                                                                                  
aaaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTCGCGCTCTTCTTTTAGGGAAAACAGGAGACCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCACGATAAAAc                                                                                                                                                   >  1:302735/1‑150 (MQ=255)
aaaTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAAc                                                                                                                                                   <  1:1827334/150‑1 (MQ=255)
  aTTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATAATCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACgg                                                                                                                                                 <  2:1477373/150‑1 (MQ=255)
        cccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGa                                                                                                                                           >  1:847427/1‑150 (MQ=255)
         ccccTTGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGat                                                                                                                                          <  1:736385/150‑1 (MQ=255)
         ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGTTTTATCGGGGCAGat                                                                                                                                          <  2:97160/150‑1 (MQ=255)
         ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                          >  2:1507921/1‑150 (MQ=255)
         ccccTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGat                                                                                                                                          >  1:1890362/1‑150 (MQ=255)
              ggTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAatat                                                                                                                                     >  2:22586/1‑150 (MQ=255)
                 aaaGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgtt                                                                                                                                  >  1:1676653/1‑150 (MQ=255)
                  aaGTATTTAAGCTTATATGCGTAACTCTAAATATCAATATTCCTTGGCGTATCTCTCTTCTTTTATGGAAAACAGGAGAACCGCCTAAATATCTCTATTTATAATTTAATGTTATATCTGCCTCGATAAAACGTGTCAGATAATATgttt                                                                                                                                 <  2:1231982/150‑1 (MQ=255)
                  aaGTATGTACGCTTAGAGGCCGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttt                                                                                                                                 <  1:332708/150‑1 (MQ=255)
                   cgTAATTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGAAGCGCTCTTCTTTAAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGAAAAAACGGGGCAGATAATATgtttc                                                                                                                                <  2:1622019/149‑2 (MQ=255)
                   aGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttta                                                                                                                                >  1:1329985/1‑150 (MQ=255)
                    gTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTATGGCTCTTCTTTTAGGGAAAACAGGAGATCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATgttttg                                                                                                                               <  1:907423/150‑3 (MQ=255)
                         tACGCTTAGAGGCGGAACTCTAAATCTCAATATTCCTTGTCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTAc                                                                                                                          <  2:1207442/150‑1 (MQ=255)
                         tACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGACCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCAATAAAACGGGGCAGATAATATGTTTAGTTTAc                                                                                                                          >  1:1906333/1‑150 (MQ=255)
                         tACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGCCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCAATAAAACGCGGCAGATAATATgtttactttaa                                                                                                                          >  1:1897660/1‑149 (MQ=255)
                          aCGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTATATTTATAATTTAATGTTATATCTGCCCCCATAAAACGGGGCAGATAATATGTTTAGTTTACt                                                                                                                         >  2:1859502/1‑150 (MQ=255)
                           cGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTa                                                                                                                        >  2:966094/1‑150 (MQ=255)
                           cGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTa                                                                                                                        >  2:522509/1‑150 (MQ=255)
                             cTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAAc                                                                                                                      >  2:1705408/1‑150 (MQ=255)
                             cTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAAc                                                                                                                      <  1:1543761/150‑1 (MQ=255)
                               tAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACgg                                                                                                                    <  1:1290064/150‑1 (MQ=255)
                               tAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACgg                                                                                                                    >  2:667709/1‑150 (MQ=255)
                                  agGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCa                                                                                                                 <  1:1717903/150‑1 (MQ=255)
                                  agGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCa                                                                                                                 <  2:1893139/150‑1 (MQ=255)
                                      ggAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATATTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCAtttt                                                                                                             >  1:1380176/1‑150 (MQ=255)
                                        aaatctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  2:1159624/147‑1 (MQ=255)
                                        aaCTCTAAATATCAATATTCCTGGGCGTAGCGGTCTTCTTTTAGGCAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  2:841345/150‑1 (MQ=255)
                                        aaCTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTAATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGc                                                                                                           <  2:547089/150‑1 (MQ=255)
                                          ctctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAg                                                                                                         <  1:2050765/150‑1 (MQ=255)
                                           tctAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTAATAACGGTCATTTTGCAGt                                                                                                        >  1:1960326/1‑150 (MQ=255)
                                             tAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGa                                                                                                      <  2:282737/150‑1 (MQ=255)
                                             tAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATAAGTTTAGTTTACTAACGGTCATTTTGCAGTGa                                                                                                      >  1:142198/1‑150 (MQ=255)
                                              aaaTATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGaa                                                                                                     >  1:1093493/1‑150 (MQ=255)
                                               aaTATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAg                                                                                                    <  2:802609/150‑1 (MQ=255)
                                                 tatCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAACCAGTAGAACCGCCGAACTATCTCTATTGTTAATTTAATGTTATATCTTCCACGATAAAACGTGGCAGATAATATGTTTATTTTACTTACTGTCATTTTGCAGTGAAGcc                                                                                                  >  1:309168/1‑150 (MQ=255)
                                                  atCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCa                                                                                                 >  1:528740/1‑150 (MQ=255)
                                                    cAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAtt                                                                                               <  2:1266439/150‑1 (MQ=255)
                                                         ttCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTg                                                                                                            >  1:992541/1‑132 (MQ=255)
                                                         ttCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTg                                                                                          <  1:627908/150‑1 (MQ=255)
                                                           ccTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGACATTTTGCAGTTAAGCCATTTACTGtt                                                                                        >  2:1861374/1‑150 (MQ=255)
                                                            cTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGttt                                                                                       >  2:1240589/1‑150 (MQ=255)
                                                                gCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTa                                                                                   <  2:1607751/150‑1 (MQ=255)
                                                                 cGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTAt                                                                                  <  2:1013668/150‑1 (MQ=255)
                                                                      cgcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGAcc                                                                             >  2:1466524/1‑150 (MQ=255)
                                                                      cgcTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGAcc                                                                             <  1:1493272/150‑1 (MQ=255)
                                                                            tcttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATaa                                                                       >  1:1142551/1‑150 (MQ=255)
                                                                            tcttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATaa                                                                       >  2:1705815/1‑150 (MQ=255)
                                                                             cttTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAAt                                                                      >  2:1126741/1‑150 (MQ=255)
                                                                                   gggAAAACAGGAGAACCGCCTAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtc                                                                <  2:640566/150‑1 (MQ=255)
                                                                                   gggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtc                                                                <  1:4553/150‑1 (MQ=255)
                                                                                   gggAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCACCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGTTCATTTTGAAGTGAAGCAATTTCCTGTTTTTTATCGACGAGATAATCTGTtc                                                                >  2:330686/1‑150 (MQ=255)
                                                                                     gAAAACATGAGAACCGCCTAACTATCTCTATTTATAATTTAATTTTATCTCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtctc                                                              <  2:191796/150‑1 (MQ=255)
                                                                                     gAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtctc                                                              >  2:1818927/1‑150 (MQ=255)
                                                                                     gAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtctc                                                              <  1:22586/150‑1 (MQ=255)
                                                                                     gAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTtctc                                                              <  1:1821287/150‑1 (MQ=255)
                                                                                      aaaaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCTGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTctct                                                             <  1:2002684/150‑1 (MQ=255)
                                                                                      aaaaCAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTctct                                                             >  1:423055/1‑150 (MQ=255)
                                                                                          cAGGAGAACCTCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATg                                                         <  1:1167769/150‑1 (MQ=255)
                                                                                          cAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATg                                                         <  2:1029162/150‑1 (MQ=255)
                                                                                          aaGGAGAACCGCCGATCTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATg                                                         <  1:1364407/149‑1 (MQ=255)
                                                                                          aaGGAGAACCGCCGAACTATCTCTATTTATAATTTTATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATg                                                         <  1:416363/149‑1 (MQ=255)
                                                                                           aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGt                                                        <  1:910709/150‑1 (MQ=255)
                                                                                           aGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGt                                                        >  1:1382792/1‑150 (MQ=255)
                                                                                            ggAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATg                                                         >  1:1879187/1‑148 (MQ=255)
                                                                                             gagaACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTa                                                      <  2:896851/150‑1 (MQ=255)
                                                                                                 aCCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATATTCTGTTCTCTAATGTTAAATc                                                  >  1:1632223/1‑150 (MQ=255)
                                                                                                  ccgccgAACTATCTCTATTTATTATTTAATGTTATATCTGCCCCGATAAATCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTcc                                                 <  2:2033781/150‑1 (MQ=255)
                                                                                                     ccgAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTccccc                                              >  1:1632388/1‑150 (MQ=255)
                                                                                                      cgAACTATCTCTTTTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCt                                             <  2:376683/150‑1 (MQ=255)
                                                                                                      cgAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATAAGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCt                                             <  2:1385027/150‑1 (MQ=255)
                                                                                                          cTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAAcc                                         <  1:1041529/150‑1 (MQ=255)
                                                                                                            aTCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGAGGCAGATAATATGTTAAGTTTACTAACAGTCATTTTGCAGTGAAGCCATTTAATGTTTTTTATCGACAAGATAATCTGTTCACTAATGTTAACTCCCCCTAACCTg                                       >  2:1307731/1‑150 (MQ=255)
                                                                                                               tctATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATATGTTCTCTAATGTTAACTCCCCCTAAACTGTTg                                    >  2:1301392/1‑150 (MQ=255)
                                                                                                                   tttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCttt                                <  2:1890362/150‑1 (MQ=255)
                                                                                                                    ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                               >  1:1669048/1‑150 (MQ=255)
                                                                                                                    ttATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTa                               <  2:1482304/150‑1 (MQ=255)
                                                                                                                      ttaATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                             <  1:1167712/149‑1 (MQ=255)
                                                                                                                      ataATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGt                             >  2:829672/1‑150 (MQ=255)
                                                                                                                        aaTTTAATGTTATATCTTCCCCGATAAAAAGGTGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTa                           <  2:77411/150‑1 (MQ=255)
                                                                                                                         aTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTAt                          >  2:311204/1‑150 (MQ=255)
                                                                                                                         aTTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTCTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGATGCTTTAGTTAt                          >  1:978469/1‑150 (MQ=255)
                                                                                                                              aTGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCAtt                     >  2:740515/1‑150 (MQ=255)
                                                                                                                                gTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTc                   <  1:522509/150‑1 (MQ=255)
                                                                                                                                gTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCAGTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTTTTCAtttg                   >  1:270873/1‑149 (MQ=255)
                                                                                                                                gTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACCAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTc                   >  2:1759747/1‑150 (MQ=255)
                                                                                                                                  tataTCTGCCCCGCTAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCt                 >  1:1278623/1‑150 (MQ=255)
                                                                                                                                  tataTCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCt                 >  1:161318/1‑150 (MQ=255)
                                                                                                                                   atatCTGCCCGATAAAACGGTGCAGATAATATGTTTATTTTAATAACGGTCATTTTGCAGTGAAGCCATTTAATGTTTTTTATCGACCAGATAATATGTTCTCTAATGTTAAATCCCCAAAACATGTTGATTTAGTTATTCATTTCCTGt               >  2:1396557/1‑150 (MQ=255)
                                                                                                                                      tCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATATTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct             >  1:2058568/1‑150 (MQ=255)
                                                                                                                                      tCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATATTAACTCACCCTAACCTGTTGCTTTAGTTATTCATTTCCTGtct             >  1:2050553/1‑150 (MQ=255)
                                                                                                                                        ttccccGATAAAAAGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATTATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCa           <  1:280218/148‑1 (MQ=255)
                                                                                                                                              gATAAATCGGGGCAGATAATATGTTTAGATTACTAACGTTCATTTTGCAGTGAAGCCATTTACTGGTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGc     <  2:1396556/150‑1 (MQ=255)
                                                                                                                                              gATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTATCACTTTGc     >  1:826823/1‑150 (MQ=255)
                                                                                                                                               aTAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGcc    >  2:47959/1‑150 (MQ=255)
                                                                                                                                               aTAAAAAGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCTACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGcc    <  1:1061304/150‑1 (MQ=255)
                                                                                                                                                 aaaaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCtt  >  2:652110/1‑150 (MQ=255)
                                                                                                                                                 aaaaCGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCtt  <  1:1705408/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAATTATCCCCCCTGGTAAAGTATTTACGCTTAGAGGCGGAACTCTAAATATCAATATTCCTGGGCGTAGCGCTCTTCTTTTAGGGAAAACAGGAGAACCGCCGAACTATCTCTATTTATAATTTAATGTTATATCTGCCCCGATAAAACGGGGCAGATAATATGTTTAGTTTACTAACGGTCATTTTGCAGTGAAGCCATTTACTGTTTTTTATCGACCAGATAATCTGTTCTCTAATGTTAACTCCCCCTAACCTGTTGCTTTAGTTATTCATTTCCTGTCTCACTTTGCCTT  >  NC_000913/2788528‑2788822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: