BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,962,964	T→G	5.2%	T65P (ACG→CCG)	ygdB ←	DUF2509 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,962,964	0	T	G	5.2%	351.7 / 4.0	141	T65P (ACG→CCG)	ygdB	DUF2509 family protein
Reads supporting (aligned to +/- strand): ref base T (60/70); new base G (3/7); total (64/77)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

CAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCA  >  NC_000913/2962817‑2963113

cAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCt                                                                                                                                                     >  1:1135593/1‑150 (MQ=255)

  aTGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGGGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGc                                                                                                                                                   >  2:299669/1‑150 (MQ=255)

   tGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGcc                                                                                                                                                  <  1:188921/150‑1 (MQ=255)

   tGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGcc                                                                                                                                                  <  2:1747207/150‑1 (MQ=255)

     tttCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcag                                                                                                                                                >  1:1497369/1‑150 (MQ=255)

       tCCATCAATGACTTCGCCTGTTCGCCACAACGATACGCCTTCATAACCGGCTATCAATAAGGCTTCATTATCTGCCAGTAAACGCACTCACACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagca                                                                                                                                              >  2:378397/1‑150 (MQ=255)

       tCCATCAATGACTTCGCCTGTACGCCACAACGAAACGCCTTCAAAACCGGCAATCAATAAGGCTTCATTATCTTCCAGTAAACGCAAACAAACCTGGTCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagca                                                                                                                                              <  2:355008/150‑1 (MQ=255)

         cATCAATGACTTCGGCTGTTCGCCACAACGAAACTCCTTCATTAGCGGCAATCAATTACGCTTCATTATCTGCCAGTAATCGCAAACAAACCTTGGCATCGGTTTCATCGTACTGCGTGCTCTGAACTGCTGTCTGCGTCTGCCAGCTGt                                                                                                                                            <  2:268298/150‑1 (MQ=255)

           tCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTTAACTGCTGGCTGCGTCTGCCAGCAGTGc                                                                                                                                          <  2:1795162/150‑1 (MQ=255)

            cAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCa                                                                                                                                         >  1:797389/1‑150 (MQ=255)

               tGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttt                                                                                                                                      >  1:1706250/1‑150 (MQ=255)

                gACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAtttt                                                                                                                                     <  1:578789/150‑1 (MQ=255)

                   ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTcc                                                                                                                                  <  2:487159/150‑1 (MQ=255)

                   ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCAGCCAGTAAACGCATACAAAGCTGGGCTTCGGTTTCTGCGTACTGCGAGCACTGATCTGCTGGCTGCGTCTGCCAGCATTGCCTTTTTcc                                                                                                                                  >  2:1324697/1‑150 (MQ=255)

                     ggCCTGTTCGCCACAACGATACGGCTTCATAACCGGGAATCAATACGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcccc                                                                                                                                <  2:1561519/149‑1 (MQ=255)

                      gCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCa                                                                                                                               >  1:1166949/1‑150 (MQ=255)

                        cTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACg                                                                                                                             >  1:470298/1‑150 (MQ=255)

                          gTTCGCCACAACGAAACGCCTTCATCACAGGCAATCATTAAGGCTTCATTATCTGCCACTAAACGCAAACAAACCTGGGCCTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGcc                                                                                                                           <  1:377712/150‑1 (MQ=255)

                          gTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGcc                                                                                                                           <  2:1729442/150‑1 (MQ=255)

                          gTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGcc                                                                                                                           >  1:319904/1‑150 (MQ=255)

                              gCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTGAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgcg                                                                                                                       >  2:42609/1‑150 (MQ=255)

                                    aCGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACt                                                                                                                 <  2:1214659/150‑1 (MQ=255)

                                     cGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCAAGCACTGAACTACTGGCTGCGTCTGCCAGCAGTGCATATTTCCCCACGCCAGCGCCGACTg                                                                                                                >  1:722870/1‑150 (MQ=255)

                                      gAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGa                                                                                                               <  2:858972/150‑1 (MQ=255)

                                        aaCGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAAc                                                                                                             <  1:1238832/150‑1 (MQ=255)

                                          cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTTAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGa                                                                                                           >  1:1436949/1‑150 (MQ=255)

                                          cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGCCTGAACGa                                                                                                           >  2:1534535/1‑150 (MQ=255)

                                            ccTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAAACTGGGCATCGGTTTCAGCGTACTGCGAGTACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                         <  1:1385029/150‑1 (MQ=255)

                                             cTTCATAACCGGCAATCACTAAGGCTTCATTATCTTCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGGACTGCGAGCACTGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATgg                                                                                                        <  1:1496239/150‑1 (MQ=255)

                                              ttCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGc                                                                                                       >  2:686271/1‑150 (MQ=255)

                                              ttCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGc                                                                                                       <  2:769714/150‑1 (MQ=255)

                                              ttCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGc                                                                                                       >  2:677201/1‑150 (MQ=255)

                                               tCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGAGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGcc                                                                                                      >  2:1144156/1‑150 (MQ=255)

                                                 aTAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTg                                                                                                    <  1:1245177/150‑1 (MQ=255)

                                                  tAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg                                                                                                   >  1:1667715/1‑150 (MQ=255)

                                                  tAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTgg                                                                                                   >  2:766730/1‑150 (MQ=255)

                                                    aCCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcg                                                                                                 >  2:111720/1‑150 (MQ=255)

                                                    aCCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcg                                                                                                 <  2:1620996/150‑1 (MQ=255)

                                                     ccGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcgg                                                                                                >  1:1842216/1‑150 (MQ=255)

                                                            tcactAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTGCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtg                                                                                         <  2:1003115/150‑1 (MQ=255)

                                                            tcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtg                                                                                         <  1:1788843/150‑1 (MQ=255)

                                                              aatAAGGCTTCATTATCTGCCAGTAAACGCAAACAACCCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgac                                                                                       <  1:1079924/150‑1 (MQ=255)

                                                              aatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgac                                                                                       <  1:165929/150‑1 (MQ=255)

                                                                  aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTTCTTGATGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGCCTGAACGATGGCCTGGCGCCGCACtgcctgac                                                                                   >  1:122838/1‑150 (MQ=255)

                                                                     cTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCTGCGCAATGACTGACttt                                                                                >  2:796538/1‑150 (MQ=255)

                                                                        cATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGTTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCCCGCCCGCGCCGACTGAACGATGGCCTGGCGGCGAAATGACTGACTTTCCa                                                                             >  1:2099269/1‑150 (MQ=255)

                                                                        cATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCa                                                                             <  1:620335/150‑1 (MQ=255)

                                                                         aTTCTCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCAt                                                                            <  2:190525/150‑1 (MQ=255)

                                                                         aTTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCACAATGACTGACTTTCCAt                                                                            <  2:2049084/150‑1 (MQ=255)

                                                                             tCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTc                                                                        <  1:1472383/150‑1 (MQ=255)

                                                                              cTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCa                                                                       <  1:2075033/150‑1 (MQ=255)

                                                                               tGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAc                                                                      >  1:693172/1‑150 (MQ=255)

                                                                                gCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcg                                                                     >  2:524037/1‑150 (MQ=255)

                                                                                   aGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgcgt                                                                  >  2:1663241/1‑149 (MQ=255)

                                                                                       aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGCCTTTCCATGCTCACGCGAGAgg                                                              >  2:1840672/1‑150 (MQ=255)

                                                                                       aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAgg                                                              <  2:880198/150‑1 (MQ=255)

                                                                                           caaacaaaCCTGGGCATCGGTTTCAGCGTACTGAGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCCCGCCAGCGCCGACTTAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCCCGCGAGCGGCaaa                                                          >  1:1301186/1‑150 (MQ=255)

                                                                                             aacaaacCTTGGCTTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAAc                                                        <  2:1392200/150‑1 (MQ=255)

                                                                                               caaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTg                                                      >  2:1660893/1‑150 (MQ=255)

                                                                                                aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGc                                                     <  1:1696971/150‑1 (MQ=255)

                                                                                                aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGc                                                     <  1:1144445/150‑1 (MQ=255)

                                                                                                   ccTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGAt                                                  <  1:970578/150‑1 (MQ=255)

                                                                                                    cTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATc                                                 <  1:1204850/150‑1 (MQ=255)

                                                                                                    cTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATc                                                 >  2:898688/1‑150 (MQ=255)

                                                                                                     tGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATcc                                                >  2:643813/1‑150 (MQ=255)

                                                                                                      gggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCt                                               <  1:285025/150‑1 (MQ=255)

                                                                                                        gCATCGGTTTCAGCGTTCTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGTCGGAACGCTGGCCTGGCGGCGCAATGCCTGCCTTTCCATGCGCACGCGAGCGGCACAACTGCGAGCCTGt                                             >  2:878571/1‑150 (MQ=255)

                                                                                                         cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCCGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGtt                                            >  2:503670/1‑150 (MQ=255)

                                                                                                         cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGACTGGCGGCGCAATGACTGACTTTCCATGCTAACGCGAGAGGCAAAACTGCGATCCTGtt                                            >  2:1820268/1‑150 (MQ=255)

                                                                                                         cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGtt                                            <  2:1500475/150‑1 (MQ=255)

                                                                                                          aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                           <  1:217170/150‑1 (MQ=255)

                                                                                                            cGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGc                                         <  1:433960/150‑1 (MQ=255)

                                                                                                             ggTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCt                                        <  1:1921267/150‑1 (MQ=255)

                                                                                                             ggTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCt                                        <  1:42609/150‑1 (MQ=255)

                                                                                                             ggTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCt                                        <  2:1401084/150‑1 (MQ=255)

                                                                                                               tttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCa                                      <  2:251704/150‑1 (MQ=255)

                                                                                                                  cAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGCCTGACTTTCCATGCTCTCGCGAGAGGCAAAACTGCGATCCGGTTGGCGCAGTc                                   >  2:300791/1‑150 (MQ=255)

                                                                                                                    gCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGGGCATTTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATGccg                                 >  2:1849292/1‑149 (MQ=255)

                                                                                                                      gTACTGCGAGCACTGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTg                               <  2:1623763/150‑1 (MQ=255)

                                                                                                                         cTGCGAGCACTGAACTGCTGGCTGCGTCTGCCTGCAGTGCATTTTTCCCCAGGCGAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTaa                            <  2:215636/150‑1 (MQ=255)

                                                                                                                                 aCTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAAc                    >  2:1693950/1‑150 (MQ=255)

                                                                                                                                    gAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAc                 >  2:429813/1‑150 (MQ=255)

                                                                                                                                    gAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAc                 >  2:415570/1‑150 (MQ=255)

                                                                                                                                    gAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAc                 <  1:1511371/150‑1 (MQ=255)

                                                                                                                                      acagctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc               <  1:550734/147‑1 (MQ=255)

                                                                                                                                      aCTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc               <  2:789729/150‑1 (MQ=255)

                                                                                                                                      aCTGCTGGCTGCGTCTGCCAGCAGTGCATTATTCCCCACGCCAGCGCCGACTGAACGATGCCCTGGCGGCGCTTTGACTGACTTACCATGCTCACGCGAGAGGCAAACCAGCGAGCCTGTAGGCTCATACCATGTAATACCAAACtcacc               >  1:553383/1‑146 (MQ=255)

                                                                                                                                       ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGCTGGCCGGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATCGCAAACTAcccc              >  1:1011721/1‑149 (MQ=255)

                                                                                                                                       ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCTTTCCTTGTAATAGCAAACTACCCa              <  1:82350/150‑1 (MQ=255)

                                                                                                                                       ctgctgGCAGCCGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGCCTGAACGATGGCCGGGCGGCGCAATGACTGCCTTTCCATGCTCACGCGAGAGGCAAACGTGCGCGCGTGTTGGGTCATTCGTTGTAATAGCTGACGACCCa              >  1:38783/1‑150 (MQ=255)

                                                                                                                                           gggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAc          <  2:1980098/149‑1 (MQ=255)

                                                                                                                                            ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGTCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc         <  2:1227995/150‑1 (MQ=255)

                                                                                                                                            ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc         >  1:299607/1‑150 (MQ=255)

                                                                                                                                              ctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGCCTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcag       <  2:1280424/150‑1 (MQ=255)

                                                                                                                                               tgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagc      <  2:1436949/150‑1 (MQ=255)

                                                                                                                                               tgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagc      <  1:1144156/150‑1 (MQ=255)

                                                                                                                                               tgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagc      <  2:925919/150‑1 (MQ=255)

                                                                                                                                                gCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTCCCCAAAACcagcc     >  2:193786/1‑149 (MQ=255)

                                                                                                                                                   tCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAgcagca  <  2:518871/150‑1 (MQ=255)

                                                                                                                                                   tCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGGTGGCTCATTCCTTTTAATCGCAAACTCCCCAACACCagccgcc  >  1:745126/1‑149 (MQ=255)

CAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAGCA  >  NC_000913/2962817‑2963113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`