Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,067,320 A→T 7.6% intergenic (‑147/+20) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3200AT 7.6% 294.0 / 12.4 106intergenic (‑147/+20)ygfI/yggEputative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base A (50/47);  new base T (4/4);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

Only 100 of 109 total aligned reads displayed.

CATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCG  >  NC_000913/3067171‑3067469
                                                                                                                                                     |                                                                                                                                                     
cATCCCCCTCCCCCGCATCCTTGTCCGCTTACCCGTTTCAGTCAGCCGATCCTTGATGCCCCCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCcgacga                                                                                                                                                       >  2:2046015/1‑150 (MQ=255)
   ccccctcccccGCATCCTAGTCCGCTTAACCGATTCAGTCAGCCTATCCTTGATGAAACCCCGAGCAAAGATAGGTCATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATa                                                                                                                                                    <  2:162188/150‑1 (MQ=255)
           cccGCCTCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAACGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                            <  1:442244/150‑1 (MQ=255)
           cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                            <  2:669610/150‑1 (MQ=255)
           cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                            <  1:1658442/150‑1 (MQ=255)
            ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGAGGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCt                                                                                                                                           <  2:1989482/150‑1 (MQ=255)
              gCATCCTTGACCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAACGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaa                                                                                                                                         <  1:514349/150‑2 (MQ=255)
                      gTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatta                                                                                                                                 >  2:1929389/1‑150 (MQ=255)
                         cGCTTAACCGTTTCAGTCAGCCTATCCTTGATTAAACCGCGAGCAAAGATAGTTGATTACGTCATGTTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGTCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                              <  1:1906791/150‑1 (MQ=255)
                         cGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                              >  2:1239090/1‑150 (MQ=255)
                         cGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAATAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                              <  1:1735876/150‑1 (MQ=255)
                            ttAACCGTTTCCGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                                                                           <  1:888291/150‑1 (MQ=255)
                               aCCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                                                                                                        >  2:2084751/1‑150 (MQ=255)
                                 cgattCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg                                                                                                                      >  1:1398970/4‑150 (MQ=255)
                                  gTTTCAGTCAGCCTATCCTTGATGACACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                     <  1:1907121/150‑1 (MQ=255)
                                  gTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                     <  1:1359579/150‑1 (MQ=255)
                                    ttCAGTCAGCCTATCCTTGATGAAACCGAGTGCAAATATTGGTGATTACGTGATTGTTTTACAGAATATTACAGAAAAAGGAGGCAATATCGGGTATAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                                                                                   <  1:383049/150‑1 (MQ=255)
                                      cagtcagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtt                                                                                                                 >  2:1562353/1‑150 (MQ=255)
                                       agtcagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                                                                                >  1:960218/1‑150 (MQ=255)
                                          cagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg                                                                                                             >  1:702283/1‑150 (MQ=255)
                                            gCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                                                                           >  2:1246470/1‑150 (MQ=255)
                                                 tCCTTGATGAAACCGCGAGCAATGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttg                                                                                                      <  1:1136071/150‑1 (MQ=255)
                                                  ccTTGATGAAACCGCGAGCAAAGATTGGTGATTACGTCATGGTTTTAGAGAAAATTACAGAAAAAGGAGGCAATTTCGGGTATAGGCATTAGCCCGACGCAGTCGTCGGGCTACACATTTTATGGTGCTGCAGGTGTTTTAGCGtgttgt                                                                                                     >  2:1392241/1‑150 (MQ=255)
                                                     tgatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa                                                                                                  >  1:666291/1‑150 (MQ=255)
                                                       attaaaCCGCTAGCAAAGATAGGTGATTACGTAATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGATGCAGGTGTTTTAGCGGGTTGTTGATc                                                                                                <  2:1852346/147‑1 (MQ=255)
                                                        tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACCGAAAAAGGAGGCAATCTCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                                                               <  1:1129689/150‑1 (MQ=255)
                                                        tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                                                               <  1:558205/150‑1 (MQ=255)
                                                        tgaAACCGCGAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATcc                                                                                               <  1:1202607/150‑1 (MQ=255)
                                                         gaACCCGCGAGCAAAGATAGGTGATTACGTCAGGGTTTTACAGAAAATTACAGAATAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGCCGAGTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                                                                              >  2:196120/1‑150 (MQ=255)
                                                         gaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACTAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                                                                              >  1:340317/1‑150 (MQ=255)
                                                          aaaCCGCGAGCAAAGATAGGTGATTACGTCATGTTTTTACAGAAAATTACAGAAAAAGGAGGCAAAATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATAAAATTCTCCTGCAGGTGTTTTAGCGGGTTGTTGATCcac                                                                                             >  1:1677037/1‑150 (MQ=255)
                                                                 gAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCt                                                                                      <  2:1621838/150‑1 (MQ=255)
                                                                 gAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATCCACAGGTTCt                                                                                      <  2:1103701/150‑1 (MQ=255)
                                                                     aaaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACt                                                                                  >  1:1079969/1‑150 (MQ=255)
                                                                     aaaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAGGCATTAGCCCGACGAATACGTCGGGATACAAATATTATTGTGCTGCAGTTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                                                                                 >  2:1124135/1‑150 (MQ=255)
                                                                       aGATAGGTGATTATGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCTTTATCGGGTTAAGGCTTTTGCCCGAAGAATACGTCGGGCTACATATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg                                                                                <  1:286489/150‑1 (MQ=255)
                                                                        gATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                                                                               <  2:1806323/150‑1 (MQ=255)
                                                                        gATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                                                                               >  2:1201039/1‑150 (MQ=255)
                                                                            ggTGATTACGTCATGGTTTTACAGAATATTACAGAAAAAGTACGCAATATCGGGTAAAGGCATAAGCCCGACGAATACGTCGGGCTACAAATTTTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGCTCCACAGGTTCTAACTGGTAGAc                                                                           <  2:1752046/150‑1 (MQ=255)
                                                                             gTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAcc                                                                          <  1:1742557/150‑1 (MQ=255)
                                                                               gATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcac                                                                        <  2:1836568/150‑1 (MQ=255)
                                                                               gATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcac                                                                        >  1:916484/1‑150 (MQ=255)
                                                                                   aCGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGAGGGTTGTTGATCCCCAGGTTCTAACTGGAAGACCACATCg                                                                    >  2:1660965/1‑150 (MQ=255)
                                                                                    cGTCATGGTTTTACAGAAATTTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCTGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGa                                                                   >  1:1005821/1‑150 (MQ=255)
                                                                                       cATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                                                                >  2:1969891/1‑150 (MQ=255)
                                                                                       cATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                                                                >  2:1786972/1‑150 (MQ=255)
                                                                                        aTGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                                                               <  2:391822/150‑1 (MQ=255)
                                                                                         tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGa                                                              >  2:1712980/1‑150 (MQ=255)
                                                                                          ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat                                                             <  2:1636257/150‑1 (MQ=255)
                                                                                          ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat                                                             <  1:243258/150‑1 (MQ=255)
                                                                                            tcttacagaaaattccagaaaaTGGAGGCAATATCTGGTAAAGGGATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGGTCTAACTTGAAGACCACATCGACCTGatca                                                           <  2:2057316/148‑1 (MQ=255)
                                                                                              ttacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcatc                                                         <  1:1813412/150‑1 (MQ=255)
                                                                                              ttacagaaaataacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcatc                                                         <  2:1622745/150‑1 (MQ=255)
                                                                                               tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGTGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGAtcatca                                                        <  1:1929389/150‑1 (MQ=255)
                                                                                                acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaa                                                       >  1:498848/1‑150 (MQ=255)
                                                                                                 nagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaaa                                                      >  2:869858/2‑150 (MQ=255)
                                                                                                  agaaaattacagaaacAGGAGGCAATATCGGGGAAAGGCAGTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGATCTAGCGGGGTGTGGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAAc                                                     <  1:882224/150‑1 (MQ=255)
                                                                                                  agaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAAc                                                     <  2:503467/150‑1 (MQ=255)
                                                                                                   gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt                                                    >  1:61316/1‑150 (MQ=255)
                                                                                                    aaaattacagaaaaAGGAGTCAATATCGGGTAAAGGCATTAGCCCGACTAATACGTCGTGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTg                                                   <  2:1872701/150‑1 (MQ=255)
                                                                                                     aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                  >  2:678559/1‑150 (MQ=255)
                                                                                                     aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                  <  2:1781726/150‑1 (MQ=255)
                                                                                                     aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                  <  1:592875/150‑1 (MQ=255)
                                                                                                      aattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGAGATTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATAGACCTGATCATCAAACTGaa                                                 >  1:394584/1‑150 (MQ=255)
                                                                                                            agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCgg                                           <  1:1487362/150‑1 (MQ=255)
                                                                                                            agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCgg                                           >  1:798835/1‑150 (MQ=255)
                                                                                                             gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGc                                          >  2:254315/1‑150 (MQ=255)
                                                                                                              aaaaaGGAGGCTATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTAGTGTGCTGCAGGTGTTTTAGCGGGATGTTGATCCACAGGTTCTAACTGGATGACCACATCGAGCTGTGCGTCATACTGAATAGCggac                                         >  2:1274391/1‑148 (MQ=255)
                                                                                                              aaaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGcc                                         <  1:217091/150‑1 (MQ=255)
                                                                                                               aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCt                                        >  1:2067440/1‑150 (MQ=255)
                                                                                                               aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCt                                        >  1:1795902/1‑150 (MQ=255)
                                                                                                                   ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTc                                    >  1:1321616/1‑150 (MQ=255)
                                                                                                                   ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGAAGGTCTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTc                                    >  2:35612/1‑150 (MQ=255)
                                                                                                                    gagtCAATATCGGGTAAAGGCATTAGCCCGACGAATCCGACGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATCCACAGGGGCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCg                                   <  1:100171/150‑1 (MQ=255)
                                                                                                                    gaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCTGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCg                                   <  1:1740034/150‑1 (MQ=255)
                                                                                                                     tggCAATATCGGGTAAAGGCATTTGCCCGACGAATAGGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGt                                  <  2:2057331/149‑1 (MQ=255)
                                                                                                                        cAATATCGGGTAAAGGCATTAGCCCGACGAATCCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGTAAGACCACATCGACCTTATCATCAAACTGAATAGCGGCCTGCTCGTAAg                               <  2:1137530/150‑1 (MQ=255)
                                                                                                                        aaaTATCGGGTAAAGGCATTAGACCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTAGATAAACAGGTTCTAAATGTAAGACCACATCGACCTGATCATCAACATGAATAGCCGCCTGCTCGTAAg                               >  2:1526061/2‑150 (MQ=255)
                                                                                                                          atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGtt                             >  2:1946296/1‑150 (MQ=255)
                                                                                                                               gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTg                        >  1:1478196/1‑150 (MQ=255)
                                                                                                                                    aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACTTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCgt                   >  1:231284/1‑149 (MQ=255)
                                                                                                                                    aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCgg                   <  1:2084751/150‑1 (MQ=255)
                                                                                                                                    aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGGGGTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCgg                   <  1:583206/150‑1 (MQ=255)
                                                                                                                                      ggCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGac                 >  1:1519564/1‑150 (MQ=255)
                                                                                                                                       gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGCCCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacc                >  1:887151/1‑149 (MQ=255)
                                                                                                                                         aTTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACAcc              <  1:798636/150‑1 (MQ=255)
                                                                                                                                           tAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCgg            >  1:576687/1‑150 (MQ=255)
                                                                                                                                            aGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc           >  2:481960/1‑150 (MQ=255)
                                                                                                                                            aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc           >  2:1648543/1‑150 (MQ=255)
                                                                                                                                            aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTGGATCCACAGGTTCTAACTGGATGGCGCCTTCGACCTGATCATCTAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc           >  1:363787/1‑150 (MQ=255)
                                                                                                                                             gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg          >  1:44176/1‑150 (MQ=255)
                                                                                                                                             gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGAAAGTTTCCTGGGCGGACACCGgcg          >  2:835607/1‑150 (MQ=255)
                                                                                                                                             gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg          >  2:1173043/1‑150 (MQ=255)
                                                                                                                                             gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg          <  2:735576/150‑1 (MQ=255)
                                                                                                                                              cccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcgc         >  1:1616406/1‑150 (MQ=255)
                                                                                                                                              cccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcgc         <  1:180719/150‑1 (MQ=255)
                                                                                                                                               ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGTCCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGcgcg        <  2:1398970/150‑1 (MQ=255)
                                                                                                                                               ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGcgcg        <  2:19085/150‑1 (MQ=255)
                                                                                                                                                     aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCg  <  2:340317/150‑1 (MQ=255)
                                                                                                                                                     aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCg  <  2:474767/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATCCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCG  >  NC_000913/3067171‑3067469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: