Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,067,323 A→T 8.1% intergenic (‑150/+17) ygfI ← / ← yggE putative DNA‑binding transcriptional regulator/oxidative stress defense protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,067,3230AT 8.1% 301.0 / 11.4 108intergenic (‑150/+17)ygfI/yggEputative DNA‑binding transcriptional regulator/oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base A (51/46);  new base T (5/4);  total (56/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 110 total aligned reads displayed.

CCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCT  >  NC_000913/3067174‑3067472
                                                                                                                                                     |                                                                                                                                                     
ccccctcccccGCATCCTAGTCCGCTTAACCGATTCAGTCAGCCTATCCTTGATGAAACCCCGAGCAAAGATAGGTCATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATa                                                                                                                                                       <  2:162188/150‑1 (MQ=255)
        cccGCCTCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAACGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                               <  1:442244/150‑1 (MQ=255)
        cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                               <  2:669610/150‑1 (MQ=255)
        cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGc                                                                                                                                               <  1:1658442/150‑1 (MQ=255)
        cccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGc                                                                                                                                               >  2:1898592/1‑150 (MQ=255)
         ccGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGAGGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCt                                                                                                                                              <  2:1989482/150‑1 (MQ=255)
           gCATCCTTGACCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAACGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGTATTCGTCGGGCTaa                                                                                                                                            <  1:514349/150‑2 (MQ=255)
              tCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACaaa                                                                                                                                         >  1:19085/1‑150 (MQ=255)
                   gTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAAtatta                                                                                                                                    >  2:1929389/1‑150 (MQ=255)
                      cGCTTAACCGTTTCAGTCAGCCTATCCTTGATTAAACCGCGAGCAAAGATAGTTGATTACGTCATGTTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGTCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                                 <  1:1906791/150‑1 (MQ=255)
                      cGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                                 >  2:1239090/1‑150 (MQ=255)
                      cGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCAATAGCCCGACGAATACGTCGGGCTACAAATATTATtg                                                                                                                                 <  1:1735876/150‑1 (MQ=255)
                         ttAACCGTTTCCGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgc                                                                                                                              <  1:888291/150‑1 (MQ=255)
                            aCCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGtgctgc                                                                                                                           >  2:2084751/1‑150 (MQ=255)
                              cgattCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAg                                                                                                                         >  1:1398970/4‑150 (MQ=255)
                               gTTTCAGTCAGCCTATCCTTGATGACACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                        <  1:1907121/150‑1 (MQ=255)
                               gTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                        <  1:1359579/150‑1 (MQ=255)
                               gTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACATCGGGCTACAAATATTATTGTGCTGCAgg                                                                                                                        <  1:763734/150‑1 (MQ=255)
                                 ttCAGTCAGCCTATCCTTGATGAAACCGAGTGCAAATATTGGTGATTACGTGATTGTTTTACAGAATATTACAGAAAAAGGAGGCAATATCGGGTATAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGgtg                                                                                                                      <  1:383049/150‑1 (MQ=255)
                                   cagtcagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGtt                                                                                                                    >  2:1562353/1‑150 (MQ=255)
                                    agtcagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGttt                                                                                                                   >  1:960218/1‑150 (MQ=255)
                                       cagCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAg                                                                                                                >  1:702283/1‑150 (MQ=255)
                                         gCCTATGCTTGATGAAACCGCGAGCAAAGATAGGTGAGTACGTCATGGTTTTACAGAAAAATACAGAAAAAGGAGGCAAGAACGGGTAAAGGCATTAGCCCGACGACTACCACGTGATACAAATATTATTGTGCTGAATGTGTTTTAGCg                                                                                                              >  2:269879/1‑150 (MQ=255)
                                         gCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCg                                                                                                              >  2:1246470/1‑150 (MQ=255)
                                              tCCTTGATGAAACCGCGAGCAATGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGgttg                                                                                                         <  1:1136071/150‑1 (MQ=255)
                                                  tgatgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGa                                                                                                     >  1:666291/1‑150 (MQ=255)
                                                    attaaaCCGCTAGCAAAGATAGGTGATTACGTAATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGATGCAGGTGTTTTAGCGGGTTGTTGATc                                                                                                   <  2:1852346/147‑1 (MQ=255)
                                                     tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACCGAAAAAGGAGGCAATCTCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                                                                  <  1:1129689/150‑1 (MQ=255)
                                                     tgaAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATcc                                                                                                  <  1:558205/150‑1 (MQ=255)
                                                     tgaAACCGCGAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATcc                                                                                                  <  1:1202607/150‑1 (MQ=255)
                                                      gaACCCGCGAGCAAAGATAGGTGATTACGTCAGGGTTTTACAGAAAATTACAGAATAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGCCGAGTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCca                                                                                                 >  2:196120/1‑150 (MQ=255)
                                                       aaaCCGCGAGCAAAGATAGGTGATTACGTCATGTTTTTACAGAAAATTACAGAAAAAGGAGGCAAAATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATAAAATTCTCCTGCAGGTGTTTTAGCGGGTTGTTGATCcac                                                                                                >  1:1677037/1‑150 (MQ=255)
                                                              gAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCt                                                                                         <  2:1621838/150‑1 (MQ=255)
                                                              gAGCAAAGATAGGGGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATCCACAGGTTCt                                                                                         <  2:1103701/150‑1 (MQ=255)
                                                                  aaaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACt                                                                                     >  1:1079969/1‑150 (MQ=255)
                                                                  aaaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAGGCATTAGCCCGACGAATACGTCGGGATACAAATATTATTGTGCTGCAGTTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTg                                                                                    >  2:1124135/1‑150 (MQ=255)
                                                                   aaGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGATACAAATATTATTGTGATGCAGGTGTTGTAGCGGGTTGTTGATACACAGGTTCTaaatt                                                                                    >  2:1631243/1‑147 (MQ=255)
                                                                    aGATAGGTGATTATGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCTTTATCGGGTTAAGGCTTTTGCCCGAAGAATACGTCGGGCTACATATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTgg                                                                                   <  1:286489/150‑1 (MQ=255)
                                                                     gATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                                                                                  >  2:1201039/1‑150 (MQ=255)
                                                                     gATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGa                                                                                  <  2:1806323/150‑1 (MQ=255)
                                                                         ggTGATTACGTCATGGTTTTACAGAATATTACAGAAAAAGTACGCAATATCGGGTAAAGGCATAAGCCCGACGAATACGTCGGGCTACAAATTTTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGCTCCACAGGTTCTAACTGGTAGAc                                                                              <  2:1752046/150‑1 (MQ=255)
                                                                          gTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGAcc                                                                             <  1:1742557/150‑1 (MQ=255)
                                                                            gATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACcac                                                                           <  2:1836568/150‑1 (MQ=255)
                                                                                 cGTCATGGTTTTACAGAAATTTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCTGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGa                                                                      >  1:1005821/1‑150 (MQ=255)
                                                                                    cATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                                                                   >  2:1786972/1‑150 (MQ=255)
                                                                                    cATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCt                                                                   >  2:1969891/1‑150 (MQ=255)
                                                                                     aTGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTg                                                                  <  2:391822/150‑1 (MQ=255)
                                                                                      tGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGa                                                                 >  2:1712980/1‑150 (MQ=255)
                                                                                       ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat                                                                <  2:1636257/150‑1 (MQ=255)
                                                                                       ggTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGat                                                                <  1:243258/150‑1 (MQ=255)
                                                                                         tcttacagaaaattccagaaaaTGGAGGCAATATCTGGTAAAGGGATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGGTCTAACTTGAAGACCACATCGACCTGatca                                                              <  2:2057316/148‑1 (MQ=255)
                                                                                           ttacagaaaataacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGatcatc                                                            <  2:1622745/150‑1 (MQ=255)
                                                                                            tacagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGTGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGAtcatca                                                           <  1:1929389/150‑1 (MQ=255)
                                                                                             acagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaa                                                          >  1:498848/1‑150 (MQ=255)
                                                                                              nagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaaa                                                         >  2:869858/2‑150 (MQ=255)
                                                                                              cagaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCaaa                                                         >  2:1182586/1‑150 (MQ=255)
                                                                                               agaaaattacagaaacAGGAGGCAATATCGGGGAAAGGCAGTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGATCTAGCGGGGTGTGGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAAc                                                        <  1:882224/150‑1 (MQ=255)
                                                                                               agaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAAc                                                        <  2:503467/150‑1 (MQ=255)
                                                                                                gaaaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACt                                                       >  1:61316/1‑150 (MQ=255)
                                                                                                 aaaattacagaaaaAGGAGTCAATATCGGGTAAAGGCATTAGCCCGACTAATACGTCGTGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTg                                                      <  2:1872701/150‑1 (MQ=255)
                                                                                                  aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                     <  1:592875/150‑1 (MQ=255)
                                                                                                  aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                     >  2:678559/1‑150 (MQ=255)
                                                                                                  aaattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGa                                                     <  2:1781726/150‑1 (MQ=255)
                                                                                                   aattacagaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGAGATTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATAGACCTGATCATCAAACTGaa                                                    >  1:394584/1‑150 (MQ=255)
                                                                                                         agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCGCAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCgg                                              >  1:1574608/1‑150 (MQ=255)
                                                                                                         agaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCgg                                              >  1:798835/1‑150 (MQ=255)
                                                                                                          gaaaaAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGc                                             >  2:254315/1‑150 (MQ=255)
                                                                                                           aaaaaGGAGGCTATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTAGTGTGCTGCAGGTGTTTTAGCGGGATGTTGATCCACAGGTTCTAACTGGATGACCACATCGAGCTGTGCGTCATACTGAATAGCggac                                            >  2:1274391/1‑148 (MQ=255)
                                                                                                           aaaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGcc                                            <  1:217091/150‑1 (MQ=255)
                                                                                                            aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCt                                           >  1:1795902/1‑150 (MQ=255)
                                                                                                            aaaaGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCt                                           >  1:2067440/1‑150 (MQ=255)
                                                                                                                ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTc                                       >  1:1321616/1‑150 (MQ=255)
                                                                                                                ggaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGAAGGTCTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTc                                       >  2:35612/1‑150 (MQ=255)
                                                                                                                 gagtCAATATCGGGTAAAGGCATTAGCCCGACGAATCCGACGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTTGATCCACAGGGGCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCg                                      <  1:100171/150‑1 (MQ=255)
                                                                                                                 gaggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCTGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCg                                      <  1:1740034/150‑1 (MQ=255)
                                                                                                                  tggCAATATCGGGTAAAGGCATTTGCCCGACGAATAGGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGt                                     <  2:2057331/149‑1 (MQ=255)
                                                                                                                   ggCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTa                                    <  1:438150/150‑1 (MQ=255)
                                                                                                                     cAATATCGGGTAAAGGCATTAGCCCGACGAATCCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGTAAGACCACATCGACCTTATCATCAAACTGAATAGCGGCCTGCTCGTAAg                                  <  2:1137530/150‑1 (MQ=255)
                                                                                                                     aaaTATCGGGTAAAGGCATTAGACCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTAGATAAACAGGTTCTAAATGTAAGACCACATCGACCTGATCATCAACATGAATAGCCGCCTGCTCGTAAg                                  >  2:1526061/2‑150 (MQ=255)
                                                                                                                       atatCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGtt                                >  2:1946296/1‑150 (MQ=255)
                                                                                                                            gggTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTg                           >  1:1478196/1‑150 (MQ=255)
                                                                                                                                 aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACTTCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCgt                      >  1:231284/1‑149 (MQ=255)
                                                                                                                                 aaGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCgg                      <  1:2084751/150‑1 (MQ=255)
                                                                                                                                   ggCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGac                    >  1:1519564/1‑150 (MQ=255)
                                                                                                                                    gCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGCCCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGacc                   >  1:887151/1‑149 (MQ=255)
                                                                                                                                      aTTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACAcc                 <  1:798636/150‑1 (MQ=255)
                                                                                                                                        tAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCgg               >  1:576687/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc              >  2:481960/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc              >  2:1648543/1‑150 (MQ=255)
                                                                                                                                         aGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGGTGTGGATCCACAGGTTCTAACTGGATGGCGCCTTCGACCTGATCATCTAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgc              >  1:363787/1‑150 (MQ=255)
                                                                                                                                          gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg             >  1:44176/1‑150 (MQ=255)
                                                                                                                                          gCCCGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGAAAGTTTCCTGGGCGGACACCGgcg             >  2:835607/1‑150 (MQ=255)
                                                                                                                                          gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg             >  2:1173043/1‑150 (MQ=255)
                                                                                                                                          gCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcg             <  2:735576/150‑1 (MQ=255)
                                                                                                                                           cccGACGTATTCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcgc            >  1:1616406/1‑150 (MQ=255)
                                                                                                                                           cccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGgcgc            <  1:180719/150‑1 (MQ=255)
                                                                                                                                            ccGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGTCCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGcgcg           <  2:1398970/150‑1 (MQ=255)
                                                                                                                                                  aaTACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCg     <  2:474767/150‑1 (MQ=255)
                                                                                                                                                    tACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGc   >  2:1491834/1‑150 (MQ=255)
                                                                                                                                                     aCGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCATCGGCGGACACCGGCGCGGCATCGGCt  >  2:1667390/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCCCTCCCCCGCATCCTTGTCCGCTTAACCGTTTCAGTCAGCCTATCCTTGATGAAACCGCGAGCAAAGATAGGTGATTACGTCATGGTTTTACAGAAAATTACAGAAAAAGGAGGCAATATCGGGTAAAGGCATTAGCCCGACGAATACGTCGGGCTACAAATATTATTGTGCTGCAGGTGTTTTAGCGGGTTGTTGATCCACAGGTTCTAACTGGAAGACCACATCGACCTGATCATCAAACTGAATAGCGGCCTGCTCGTAAGTTTCCTGGGCGGACACCGGCGCGGCATCGGCT  >  NC_000913/3067174‑3067472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: