Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,876 A→C 5.5% I427S (ATT→AGT)  speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8760AC 5.5% 341.4 / 7.4 120I427S (ATT→AGT) speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (47/65);  new base C (2/5);  total (50/70)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 121 total aligned reads displayed.

ATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107728‑3108025
                                                                                                                                                    |                                                                                                                                                     
atatCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAAt                                                                                                                                                      >  2:49234/1‑150 (MQ=255)
  atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                    <  1:1533085/150‑1 (MQ=255)
  atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                    <  2:1127307/150‑1 (MQ=255)
  atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                    <  1:896244/150‑1 (MQ=255)
  atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                    <  2:1188643/150‑1 (MQ=255)
   tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATATACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                   >  1:1200452/1‑150 (MQ=255)
   tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                   <  1:1794634/150‑1 (MQ=255)
    ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGGTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                                                                                                  >  2:184439/1‑150 (MQ=255)
    ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                                                                                                  <  1:1752749/150‑1 (MQ=255)
     cTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCt                                                                                                                                                 >  1:663239/1‑150 (MQ=255)
        cAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgc                                                                                                                                              >  2:717926/1‑150 (MQ=255)
        cAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCAATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgg                                                                                                                                              >  1:698711/1‑149 (MQ=255)
         aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACACTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                             <  2:803952/150‑1 (MQ=255)
         aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCCTCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                             >  2:1909107/1‑150 (MQ=255)
         aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                             >  2:480169/1‑150 (MQ=255)
          aaGCCGTGCCACTTCGCCCCCGGCTCAAACCTGAAAAAACGGCGGTCGCAGGCAAACACTGATGGCGGATACTCCTGCCCCAATTTGGCATCAACAACGGGCGGGCTAACCGGGCGGAGCCGCTTACAGCGCGCAAGATTAGCCTTgcgg                                                                                                                                            >  1:1882240/1‑149 (MQ=255)
          aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCTCAATTTGCGATCAAAAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                            >  2:1378797/1‑150 (MQ=255)
          aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGAAAATCCTGCCACAATTTGCCATCAACAACGGGCTGGATAAACGGGCTGAACAGCTTACAGCGCGCAAGAATAGCCGTgcgc                                                                                                                                            >  2:1385697/1‑150 (MQ=255)
           aGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgcg                                                                                                                                           >  2:748352/1‑150 (MQ=255)
           aGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGGCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgcg                                                                                                                                           <  1:1042188/150‑1 (MQ=255)
            gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACACCGGGCGGGCTGGGCGGGCGGTACTGCTTACAGCGCGCAAGAATCGCCTTgcgcgc                                                                                                                                          >  1:107243/1‑150 (MQ=255)
            gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                          <  1:830456/150‑1 (MQ=255)
             ccGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCt                                                                                                                                         <  2:1178443/150‑1 (MQ=255)
             ccGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCt                                                                                                                                         >  2:1066541/1‑150 (MQ=255)
              cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCtt                                                                                                                                        <  1:776794/150‑1 (MQ=255)
               gTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACTGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                       <  2:375888/150‑1 (MQ=255)
               gTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                       <  1:2038561/150‑1 (MQ=255)
                 gCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtgtaa                                                                                                                                     <  1:527315/150‑5 (MQ=255)
                   cACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtgtaagc                                                                                                                                   <  2:1761587/150‑7 (MQ=255)
                       tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGTCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGTGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                               <  1:1268391/150‑1 (MQ=255)
                       tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                               <  2:957654/150‑1 (MQ=255)
                         gCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtc                                                                                                                             <  1:764069/150‑1 (MQ=255)
                         gCCCCCGGCGCAAAACTGCAAACACGGCGGTCGCTGGCTAACACTGATGGCGGATAATCCTGCCACAATTTGCCAGCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtc                                                                                                                             <  2:354748/150‑1 (MQ=255)
                          cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                            >  1:963037/1‑150 (MQ=255)
                              cGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                        <  2:1609008/150‑1 (MQ=255)
                               ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAaca                                                                                                                       >  2:889797/1‑150 (MQ=255)
                                 cTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacaca                                                                                                                     >  2:762173/1‑150 (MQ=255)
                                 cTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacaca                                                                                                                     >  1:1460606/1‑150 (MQ=255)
                                 cTCAAAACTGAAAAAACGGCGGTCGCTGGCTAAAACTGATGTCGGATAATCCTGCCAAAATTTGCCATCAACAACGGGCGGGATAACCTGGCGGACCACCTTACATCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtctaagcaac                                                                                                                     >  1:1475273/1‑143 (MQ=255)
                                   cAATACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATATTCCTGCCACAATTTGCCGTCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                   >  2:82841/1‑150 (MQ=255)
                                   cAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGAGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                   <  1:1102937/150‑1 (MQ=255)
                                   cAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGAGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                   >  1:47464/1‑150 (MQ=255)
                                      aaCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAg                                                                                                                <  2:1865491/150‑1 (MQ=255)
                                         tGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGACCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCACTCCCTATCTCAACACACTCAGccc                                                                                                             >  1:2062851/1‑150 (MQ=255)
                                          gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcccc                                                                                                            >  2:525049/1‑149 (MQ=255)
                                          gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                                            <  2:1410492/150‑1 (MQ=255)
                                              caaCGGCGGGCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                                                        <  2:516138/149‑1 (MQ=255)
                                               aaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGcc                                                                                                       <  1:1395501/150‑1 (MQ=255)
                                                     ggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGcc                                                                                                 >  2:890313/1‑150 (MQ=255)
                                                      gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGccc                                                                                                <  2:63117/150‑1 (MQ=255)
                                                       tCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCa                                                                                               >  1:1678109/1‑150 (MQ=255)
                                                               tAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                                                       <  2:680514/150‑1 (MQ=255)
                                                                aaCACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCt                                                                                      <  1:2072124/150‑1 (MQ=255)
                                                                 acacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGAGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCACCACACTCAGCCCACAGCCGACGCCCACTATACCCtt                                                                                     >  1:1200203/1‑150 (MQ=255)
                                                                    cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                  <  1:225977/150‑1 (MQ=255)
                                                                      gATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGa                                                                                >  1:1386782/1‑150 (MQ=255)
                                                                       aTGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTTCGACCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGaa                                                                               >  2:747169/1‑150 (MQ=255)
                                                                        tGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCCACACACTCAGCCCACAGCCGACGCCCCCTCTCCCCTTCATGAAt                                                                              >  1:1891459/1‑150 (MQ=255)
                                                                        tGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAt                                                                              <  1:259577/150‑1 (MQ=255)
                                                                             gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCGGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                         <  2:910923/150‑1 (MQ=255)
                                                                              aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                                                        <  1:1198465/150‑1 (MQ=255)
                                                                              aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCAACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                                                        <  2:1723517/150‑1 (MQ=255)
                                                                                 aTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGtt                                                                     <  1:49234/150‑1 (MQ=255)
                                                                                     tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                 <  1:1688065/150‑1 (MQ=255)
                                                                                            aTTTGCCATACACCACGGGCGGGATAAACGGGCTGAACAGATTACAGCTCGCAAGAATATCCTTGCGCGCTTCAATCCCTATCTAATCACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTg                                                          <  2:288941/150‑1 (MQ=255)
                                                                                               tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                       <  1:1313050/150‑1 (MQ=255)
                                                                                                 ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                     <  1:1514373/150‑1 (MQ=255)
                                                                                                   aacaacaaCGGGAGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCATAACTCAACACACTCAGCCCACAGCCGACGCCAACTCTCCCCTTCATAAATTTTAGCGTTAACATCCAGTGCAGCaaa                                                   >  1:1274040/3‑150 (MQ=255)
                                                                                                     caacaaCGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACa                                                 >  2:433979/1‑150 (MQ=255)
                                                                                                     caacaaCGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACa                                                 <  2:1737571/150‑1 (MQ=255)
                                                                                                        caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCg                                              <  2:371127/150‑1 (MQ=255)
                                                                                                          acGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGa                                            <  1:1380621/150‑1 (MQ=255)
                                                                                                            gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                          >  2:2026770/1‑150 (MQ=255)
                                                                                                             ggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGCATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  1:1066541/150‑1 (MQ=255)
                                                                                                             ggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  2:904023/150‑1 (MQ=255)
                                                                                                              gcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGa                                        <  1:889797/150‑1 (MQ=255)
                                                                                                                gggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGaaa                                      <  1:1003766/150‑1 (MQ=255)
                                                                                                                gggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGaaa                                      >  1:380071/1‑150 (MQ=255)
                                                                                                                  gATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCACAACACACTCAGCCCCCAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAgg                                    >  2:1925630/1‑150 (MQ=255)
                                                                                                                    tAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGc                                  >  2:785733/1‑150 (MQ=255)
                                                                                                                     ataCGGGCGGAACAGCTTACAGCGCGCATGTATAGCCTTGCGCGCTTCATTCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATTGAAAGGGCt                                 <  1:894103/148‑1 (MQ=255)
                                                                                                                     aaaCGGGCGGAACAGCTTACGGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt                                 <  2:1627694/150‑1 (MQ=255)
                                                                                                                      aaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCATCACACTCAGCCCACAGCCGACGCCCACTCTGCCCTTCATGATTGTTGGCGTTAACAGCCAGTGCCGCAAACAGCGGATTGAATGGGcgg                                >  2:411000/1‑148 (MQ=255)
                                                                                                                      aaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACCCACTAAGCCCACAGCCGACGCCCACGCTCCCCTGCAAGCAATTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGcag                                >  1:2033487/1‑148 (MQ=255)
                                                                                                                      aaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCCGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTg                                >  2:1336638/1‑150 (MQ=255)
                                                                                                                      aaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTg                                >  1:1327434/1‑150 (MQ=255)
                                                                                                                          ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCCCTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAg                            >  1:1851824/1‑150 (MQ=255)
                                                                                                                          ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCTGATAGAAAGGGCTGGTAg                            <  1:2068883/150‑1 (MQ=255)
                                                                                                                          ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAg                            <  1:1507461/150‑1 (MQ=255)
                                                                                                                            cGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGaa                          <  2:1178796/150‑1 (MQ=255)
                                                                                                                                   gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                   >  1:1873919/1‑150 (MQ=255)
                                                                                                                                     ttACAGCGCGGAAGAATAGCATTGCGCGCTTCAATCCCTATCTCAACACACTCTGCCCACAGCCGTCGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGc                 <  2:1185802/150‑1 (MQ=255)
                                                                                                                                     ttACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCAGTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGc                 <  1:890313/150‑1 (MQ=255)
                                                                                                                                     ttACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGc                 <  2:7974/150‑1 (MQ=255)
                                                                                                                                      tgaaGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCCCCCCACAGCCGACGCCCACTCTCCCCTGCATGAATTTTGGCGTTAACATCCAGTGCAGCAATCAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCa                >  2:776661/4‑150 (MQ=255)
                                                                                                                                          gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa            >  1:638240/1‑150 (MQ=255)
                                                                                                                                          gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa            <  2:908258/150‑1 (MQ=255)
                                                                                                                                          gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa            >  1:969290/1‑150 (MQ=255)
                                                                                                                                             cgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCACCTTCATGAATGTTGGCGTTAACATCCAGAGCAGCAAACAGCGGATAGAAAGGGCTGGTAGTATCATGGAGCATAAAGGc         >  1:276049/1‑150 (MQ=255)
                                                                                                                                                    aTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTc  <  2:698711/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107728‑3108025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: