Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,266,132 C→A 5.2% G312C (GGT→TGT)  tdcA ← tdc operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,266,1320CA 5.2% 352.7 / 2.4 137G312C (GGT→TGT) tdcAtdc operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (78/50);  new base A (2/7);  total (80/57)
Fisher's exact test for biased strand distribution p-value = 3.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

AATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAA  >  NC_000913/3265984‑3266281
                                                                                                                                                    |                                                                                                                                                     
aataatGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCa                                                                                                                                                      >  2:2016405/1‑150 (MQ=255)
   aatGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACt                                                                                                                                                   <  1:1086890/150‑1 (MQ=255)
    atGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACtt                                                                                                                                                  >  2:1727978/1‑150 (MQ=255)
     tGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACGTc                                                                                                                                                 >  2:1372546/1‑150 (MQ=255)
      gTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACACGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCt                                                                                                                                                >  2:71043/1‑150 (MQ=255)
        catcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTAt                                                                                                                                              >  1:1137560/1‑150 (MQ=255)
          tcaATAGCAACCGGCAGATCGTATGTAATATGCTTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTATATAAAAACAAATAACTAACCAACTTCTATTa                                                                                                                                            >  2:1413151/1‑150 (MQ=255)
          tcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACAAACTTCTATTa                                                                                                                                            >  2:225516/1‑150 (MQ=255)
               aGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGc                                                                                                                                       <  1:1278348/150‑1 (MQ=255)
                gCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGcc                                                                                                                                      >  1:1042969/1‑150 (MQ=255)
                  aaCCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGGGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCtt                                                                                                                                    <  2:724961/150‑1 (MQ=255)
                   aCCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTc                                                                                                                                   >  1:1085348/1‑150 (MQ=255)
                      ggCAGATCGTATGTAATATGCATTATACACCCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACCAATAACTACCCAACTTCTATTAATTGCCTTCGta                                                                                                                                >  1:1619373/1‑149 (MQ=255)
                      ggCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTc                                                                                                                                <  1:1033929/150‑1 (MQ=255)
                      ggCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTc                                                                                                                                >  1:1827666/1‑150 (MQ=255)
                      ggCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTc                                                                                                                                <  1:1548395/150‑1 (MQ=255)
                      ggCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAAATAACCAACTTCTATTAATTGCCTTCGTc                                                                                                                                >  2:1571799/1‑150 (MQ=255)
                        cAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCTTCTa                                                                                                                              >  2:1999266/1‑150 (MQ=255)
                         aGATCGTATGTATTATTCATTATAAAACATATTCGAATGAATTAAATATGTATGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTac                                                                                                                             <  1:407643/150‑1 (MQ=255)
                          gATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTACCCAACTTCTATTAATTGCCTTCTTCTaca                                                                                                                            >  1:2035672/1‑150 (MQ=255)
                            tCGTAAGGAATATGCATTATAAAAACTCTTCGCCTGAATTAAATATGTAGGTACCCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                                          <  2:1273316/150‑1 (MQ=255)
                              gTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCa                                                                                                                        <  2:1707864/150‑1 (MQ=255)
                               tATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCAt                                                                                                                       >  1:1738726/1‑150 (MQ=255)
                               tATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCAt                                                                                                                       <  1:602402/150‑1 (MQ=255)
                                   tAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATTATTAAAAAAGTTATTTTTATTTCCTTGTTCTACACCCATtata                                                                                                                   <  2:1538546/150‑1 (MQ=255)
                                      tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGa                                                                                                                <  2:1989158/150‑1 (MQ=255)
                                       atGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGAt                                                                                                               <  1:711788/150‑1 (MQ=255)
                                        tGCATTATAAAACCTCTTCGCCTGAATTATATATGTAGGGAGCCGGAACCGACGCCGGCAAGACAGACAGGTGGGATATTTAAGTTTAAATAAAAACACATCTCTAACCGACTTCTATTAAGTGCCTTCGTCTACTCCCATTATAAGATg                                                                                                              >  2:292893/1‑150 (MQ=255)
                                        tGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATg                                                                                                              <  2:2087576/150‑1 (MQ=255)
                                           aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAAt                                                                                                           <  1:1400883/150‑1 (MQ=255)
                                              ataAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGCCAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAct                                                                                                        >  2:1097709/1‑150 (MQ=255)
                                              ataAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAATTTTTTTTTAATGTTATTTTTATTTCCTTGTTCTACACCCATTATAAGATGAATAct                                                                                                        <  2:81292/150‑1 (MQ=255)
                                               taAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATActc                                                                                                       <  2:739331/150‑1 (MQ=255)
                                                   acatctTCGCCTTAATTAAATATTTAGGTAACCGTAACCGACACCTGAAAGACAGAAAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTg                                                                                                   <  1:358827/147‑1 (MQ=255)
                                                       ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTTGATTATTTATGTTTAAATAAAAACATTTTTTTTTTAATGTTATTTTTATTTCCGTGTTCTACACCCATTATAAGATGAATACTCTTTGGCTa                                                                                               <  2:1438275/150‑1 (MQ=255)
                                                          cGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAAGAAATAACTAACCAACTACTATTAATTCCCTTCCTCTACACCCATTATAAGATGAATACTCTTTGCCTAAtt                                                                                            >  2:1038562/1‑150 (MQ=255)
                                                          cGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCTTTATAAGATGAATACTCTTTGGCTAAtt                                                                                            >  2:711872/1‑150 (MQ=255)
                                                           gCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAACAACAAATAAATAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTc                                                                                           >  1:1501535/1‑150 (MQ=255)
                                                           gCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAATCTTTGGCTAATTc                                                                                           >  1:1494403/1‑150 (MQ=255)
                                                           gCCTGAATTAAATATGTAGGTAACAGTAACCGCCACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATACCTAACCAACTTCTATTTATTGCCTTCGTCTACACACATTATAAGATGAATACTCTTTGGCTAATTc                                                                                           >  1:343837/1‑150 (MQ=255)
                                                            ccTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGGCAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTcc                                                                                          >  1:818537/1‑150 (MQ=255)
                                                                     aaTATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAAACAACTTCTATTAATTTCCTTCTTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAAcc                                                                                 <  2:860547/150‑1 (MQ=255)
                                                                         tGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACACATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtg                                                                             >  1:847949/1‑150 (MQ=255)
                                                                          gTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtgc                                                                            >  1:448410/1‑150 (MQ=255)
                                                                             ggTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtgctgc                                                                         >  2:1890764/1‑150 (MQ=255)
                                                                              gtaaccgtaaccGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAATTAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATgctgct                                                                        >  1:941239/1‑150 (MQ=255)
                                                                                 accgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTACtttt                                                                     >  1:1641764/1‑150 (MQ=255)
                                                                                  ccgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCttttt                                                                    >  1:528703/1‑150 (MQ=255)
                                                                                   cgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCtttttt                                                                   >  1:628592/1‑150 (MQ=255)
                                                                                      aaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAAt                                                                >  1:869717/1‑150 (MQ=255)
                                                                                       accgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATa                                                               >  2:284475/1‑150 (MQ=255)
                                                                                       accgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTATTTTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCATCAAAACCGATGCTGCTTTTTTAATa                                                               <  2:1554927/150‑1 (MQ=255)
                                                                                        ccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATAc                                                              >  1:1015021/1‑150 (MQ=255)
                                                                                        ccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATAc                                                              <  2:1492281/150‑1 (MQ=255)
                                                                                          gACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGa                                                            >  2:1272409/1‑150 (MQ=255)
                                                                                              ccTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAAt                                                        >  2:1157757/1‑150 (MQ=255)
                                                                                              ccTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAAt                                                        >  2:971027/1‑150 (MQ=255)
                                                                                                   aaGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAAttttta                                                   >  1:619399/1‑149 (MQ=255)
                                                                                                   aaGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTc                                                   >  2:1540062/1‑150 (MQ=255)
                                                                                                    agacagacAGGTGGATTATTTATGTTTAAATAAAAACAAATATATAATAATGTTATTTTTATTTCCTTGTTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCg                                                  <  2:35031/150‑1 (MQ=255)
                                                                                                    agacagacAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCg                                                  <  2:119600/150‑1 (MQ=255)
                                                                                                     gacagacaGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGa                                                 >  2:963787/1‑150 (MQ=255)
                                                                                                      acagacagGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGAc                                                <  1:1694175/150‑1 (MQ=255)
                                                                                                        atacagGTGGATTATTTAAGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTGTAATACGATAATTTTTCGACCa                                              <  1:117742/148‑1 (MQ=255)
                                                                                                         gacagGTTGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCAt                                             <  1:248327/150‑1 (MQ=255)
                                                                                                           cagGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATAc                                           <  1:1842336/150‑1 (MQ=255)
                                                                                                             ggTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGGCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGTCCATAccc                                         >  1:339612/1‑149 (MQ=255)
                                                                                                              gTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACcgc                                        >  1:1699888/1‑150 (MQ=255)
                                                                                                                  attattTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCa                                    <  2:146156/150‑1 (MQ=255)
                                                                                                                  attattTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAAAACTCTTTGGCTAATTCAACCAAAACCGATGCTGATTTTTTAAAACTATAATTTTTCGACCATACCGCGGCa                                    >  1:1112931/1‑150 (MQ=255)
                                                                                                                   ttatttatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCat                                   <  1:1097709/150‑1 (MQ=255)
                                                                                                                     atttatGTTTAAATAAAAACAATTTTTTATAAATCTTATTTTTATTTCCTTCTTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCatat                                 <  2:24633/150‑1 (MQ=255)
                                                                                                                      tttatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACACATTATAAGATGAATACTCTTTCGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACAATACCGCGGCATAtt                                >  1:1836318/1‑150 (MQ=255)
                                                                                                                         atgagtaaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACACATTATATGTTGATAACACTTGGGGTAAATCCAGCAAAAGCGGGGCTGCTGTTTTAATACGAATCTTTTTCTACCAAAACACGGCATTTtgtg                             >  2:388740/6‑150 (MQ=255)
                                                                                                                         atGTTTAAATAAAAACAAATAACTAACCAACTTCTTTTAATTGCCTTAGTCTAGAGCCATTATAATATGAATACTCTTTGGATAATTACACCAAAACAGATGCTGCTTTTTTAATACGAAAAATTTTCGACCATACAGCTGCATATtgtg                             >  1:164855/1‑150 (MQ=255)
                                                                                                                          tGTTTAAATAAAAACATATAAGTAACAAACTTCTATTTTTTGCCTTCGTCTACACCCATTATAAGATGATTTCTCTTTGTCTAATTCCAGCAAAACCGATGCTGCTTTTATAATACGATAATTTTTCGACCATACCGCGGCATATTGTGc                            <  1:118057/150‑1 (MQ=255)
                                                                                                                              taaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATATGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcaca                        >  2:205689/1‑150 (MQ=255)
                                                                                                                              taaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcaca                        >  2:517191/1‑150 (MQ=255)
                                                                                                                              taaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcaca                        <  2:1727402/150‑1 (MQ=255)
                                                                                                                              taaataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcaca                        >  1:1430105/1‑150 (MQ=255)
                                                                                                                               aaataaaAACAAATAACTAACCAAATTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTATTTTAATACGATAATTTTTCGACCATACCGCTGCATATTGTGACACAg                       >  2:509773/1‑150 (MQ=255)
                                                                                                                               aaaaaaaaACAAAAAACTACCCAACTTCTATTAATTGCCTTCGTCTACACACATAAAAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGAAAATTTATCGACCATACCGCGGCATATAGTGCCACAg                       <  2:878923/150‑1 (MQ=255)
                                                                                                                                aataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCTACCATACCGCGGCATATTGTGCCACAgg                      >  1:74902/1‑150 (MQ=255)
                                                                                                                                aataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAgg                      <  1:1979672/150‑1 (MQ=255)
                                                                                                                                 ataaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGt                     <  2:1927702/150‑1 (MQ=255)
                                                                                                                                  taaaAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTa                    >  2:157726/1‑150 (MQ=255)
                                                                                                                                   aaaaaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGCCCATACCGCGGCATATTGTGCCACAGGTaa                   >  2:2052562/1‑150 (MQ=255)
                                                                                                                                    aaaaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAAt                  >  2:1790755/1‑150 (MQ=255)
                                                                                                                                         aaataaatAACCAACTTCTATTAATTGCCTTGTTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTttc             <  2:51867/150‑1 (MQ=255)
                                                                                                                                            taactaacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTttcttc          >  2:1796410/1‑150 (MQ=255)
                                                                                                                                              actaacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCaa        <  1:1624207/150‑1 (MQ=255)
                                                                                                                                              actaacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCaa        >  1:955911/1‑150 (MQ=255)
                                                                                                                                               ctaacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAAc       <  1:1737394/150‑1 (MQ=255)
                                                                                                                                                taacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATTATTTTTCGCCCTTACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAAcc      >  2:1959863/1‑150 (MQ=255)
                                                                                                                                                 aacCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGCTGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACAATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCg     >  2:1203657/1‑150 (MQ=255)
                                                                                                                                                  acCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCgg    <  2:390370/150‑1 (MQ=255)
                                                                                                                                                  acCAACTTCTATTAATTGCCTTCCTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAACACCGATGCTGCTTTTTTAATTCGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCgg    <  2:266579/150‑1 (MQ=255)
                                                                                                                                                   ccAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGa   <  1:1727978/150‑1 (MQ=255)
                                                                                                                                                   ccAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGa   >  1:721179/1‑150 (MQ=255)
                                                                                                                                                    cAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGaa  <  1:1966478/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATAATGTCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACAGGTAATGTTTCTTCAACCGGAA  >  NC_000913/3265984‑3266281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: