Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,621 C→G 5.3% noncoding (35/76 nt) thrV ← tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,6210CG 5.3% 289.4 / 4.0 91noncoding (35/76 nt)thrVtRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (41/45);  new base G (2/3);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGC  >  NC_000913/3423476‑3423770
                                                                                                                                                 |                                                                                                                                                     
cTACGGCGTGTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCacg                                                                                                                                                   <  2:1310706/150‑3 (MQ=255)
 tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCAAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAgg                                                                                                                                                  <  1:978039/150‑1 (MQ=255)
  tcGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                                 <  1:1251560/149‑1 (MQ=255)
    ggCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTg                                                                                                                                               <  1:1415520/150‑1 (MQ=255)
    ggCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGGGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTg                                                                                                                                               <  2:342714/150‑1 (MQ=255)
     gCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTAACCAAGGGTgc                                                                                                                                              <  2:689998/150‑1 (MQ=255)
      cGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgag                                                                                                                                             <  1:348287/150‑3 (MQ=255)
      cGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgag                                                                                                                                             <  2:1154718/150‑3 (MQ=255)
       gTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTTCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACGCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTgagg                                                                                                                                            <  2:1489593/150‑4 (MQ=37)
        ttgcACTTCTGAGTTCGGCCTGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGct                                                                                                                                           <  2:262149/147‑1 (MQ=255)
          tCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGctct                                                                                                                                         <  1:1284488/150‑1 (MQ=255)
            ccTTCTGCGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAACAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                       <  2:1520208/149‑1 (MQ=255)
            aCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                       <  2:1687073/150‑1 (MQ=255)
            aCTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                       <  1:1776171/150‑1 (MQ=255)
             cTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAcc                                                                                                                                      <  2:574981/150‑1 (MQ=255)
             cTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAcc                                                                                                                                      <  1:1528094/150‑1 (MQ=255)
                cTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                                                                                                   <  1:198894/150‑1 (MQ=255)
                 tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTTCTGCCGCCAGACAAATTCTTTTCTATTCTGCCTAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCATGGGTGCGCTCTACCAACt                                                                                                                                  <  2:1796059/150‑1 (MQ=255)
                  gAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTGTAATCTGCCGAACTTTACCCTAAAAAGTGGTGCTGAAACCCAGAGTCGAAATGGGGACCTCACCCTTACCAAGGGTGCGATGTACCAACTg                                                                                                                                 >  1:1163037/1‑150 (MQ=38)
                    gTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                               <  2:1847894/150‑1 (MQ=255)
                    gTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                               >  1:847611/1‑150 (MQ=255)
                      tCGGCATGGGGGCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                                                                                                             <  1:176246/150‑1 (MQ=255)
                       gggCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCa                                                                                                                            <  1:990708/149‑1 (MQ=255)
                       cGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCa                                                                                                                            <  2:200233/150‑1 (MQ=255)
                         gCAGGGGGTCAGGTGGGACCACCGCGCTACTGCCGCAAGACAATTTCTTGTCTACTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTCCCAAGGGTGCGCTCTACCAACTGAGCCata                                                                                                                          <  1:998361/150‑1 (MQ=37)
                            gggggTCAGTTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGGCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                                                                                                       <  1:1510707/149‑1 (MQ=255)
                             ggggTCAGGTGGGACCACCGCGCTACTGCCGCCAGTCAAATTCTTTTCTAATCTGCCGAACTTTAACCTAATATGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCGTATCAg                                                                                                                      <  1:1738815/150‑1 (MQ=255)
                                 tCAGGTGGGACCACCGCTCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACg                                                                                                                  <  2:778746/150‑1 (MQ=255)
                                  cAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAGCCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCGCCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGc                                                                                                                 <  1:1112786/150‑1 (MQ=255)
                                   aGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCt                                                                                                                >  1:491546/1‑150 (MQ=255)
                                       gggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAt                                                                                                            >  2:10823/1‑150 (MQ=255)
                                       gggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAt                                                                                                            <  1:505877/150‑1 (MQ=255)
                                        ggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAtt                                                                                                           <  2:1779428/150‑1 (MQ=255)
                                        ggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAtt                                                                                                           <  2:1733231/150‑1 (MQ=255)
                                          accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTTCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                         <  1:1295903/150‑1 (MQ=255)
                                           ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                        <  1:1608485/150‑1 (MQ=255)
                                           ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                        <  2:541153/150‑1 (MQ=255)
                                            caccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGAt                                                                                                       >  2:1859862/1‑150 (MQ=255)
                                             accGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATg                                                                                                      <  2:468721/150‑1 (MQ=255)
                                               cgcgCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGcc                                                                                                    <  2:550038/150‑1 (MQ=255)
                                                gcgcTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCt                                                                                                   >  2:1880500/1‑150 (MQ=255)
                                                 ggcTACTGCCGCCAGACAAATTCTTATCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTg                                                                                                  <  1:1409529/149‑1 (MQ=255)
                                                 cgcTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTg                                                                                                  <  1:2061318/150‑1 (MQ=255)
                                                   cTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGc                                                                                                <  1:841546/150‑1 (MQ=255)
                                                      cTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGTTGCTGATACCCAGAGGCGAACTGGGGACCTCACCCTTACCAAGGGTGCTCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGt                                                                                             <  2:15006/150‑1 (MQ=255)
                                                      cTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGTTGCGCTCTACCAACTGAGACATATCAGCACGCTAAATTTGATGCCTGGCAGt                                                                                             >  1:383851/1‑150 (MQ=255)
                                                        gccgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAGTTGATGCCTGGCAGTTc                                                                                           >  2:811339/1‑150 (MQ=255)
                                                        gccgacAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTc                                                                                           <  2:1426506/150‑1 (MQ=255)
                                                          cgccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                         >  1:863346/1‑150 (MQ=255)
                                                           gccAGACAAATTCTTTTCTATTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                        <  1:2066733/150‑1 (MQ=255)
                                                           gccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                        <  1:661908/150‑1 (MQ=255)
                                                           gccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGAGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGCGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                        >  2:719522/1‑150 (MQ=255)
                                                               gACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTActc                                                                                    >  1:710121/1‑150 (MQ=255)
                                                                   aaTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGc                                                                                >  1:126343/1‑150 (MQ=255)
                                                                    aTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCa                                                                               >  2:528511/1‑150 (MQ=255)
                                                                       cTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                            >  2:2103285/1‑150 (MQ=255)
                                                                       cTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCACTTCCCTACTCTCGCATgg                                                                            >  2:1047651/1‑150 (MQ=255)
                                                                        ttttCTAATCTGCCGAACTTTAACCTAAAAAGTTGTGCTGATACCCAGAGGCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCGCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                           <  2:691484/150‑1 (MQ=255)
                                                                             tAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAc                                                                      >  1:1039728/1‑150 (MQ=255)
                                                                                    ccGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac                                                               >  2:1088222/1‑150 (MQ=255)
                                                                                     cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCCGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                              >  2:1465667/1‑150 (MQ=255)
                                                                                       aaCCTTACCCTAACCAGGTGTGGTGATACCCAGAGGCGAACTGCGGACCTCACCCTTACCAAGGGTGCGCTCAACCAACTCCGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAc                                                            <  1:10040/150‑1 (MQ=25)
                                                                                             aaCCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                                      >  1:179853/1‑150 (MQ=255)
                                                                                                cTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACAAAGGGTGCGCTCTACCAACTGAGCCATATCCGCACGCTAAAATAGATGCCTTTCAGTTACCTTCTCTAGCAAGGGTAGACCCCACAAAACCATCGgcgc                                                   >  1:117799/1‑150 (MQ=21)
                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAGCTGGGGCCCTCACCCTTAGCAAGGGTGCGCTCTACAAACAGCGCCATAACAGCCCGCTTAAATTGATGCCTGGCAGTGCCCTACTCTCGCATGGGGCGTCGCCACACGACCTACCGCGCt                                                  >  1:1788240/1‑150 (MQ=255)
                                                                                                 tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGCCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCt                                                  >  1:1015610/1‑150 (MQ=255)
                                                                                                   aaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAc                                                <  1:10823/150‑1 (MQ=255)
                                                                                                           tgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTc                                        >  2:1484813/1‑150 (MQ=255)
                                                                                                           tgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCAACACTACCATCGGCGCTACGGCGTGTc                                        >  2:579186/1‑150 (MQ=255)
                                                                                                            gCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCCACTGAGCCATATCAGCACGCTAAATTTGATGCCTGTCAGTTCCCTACTCTCGCATGGGGAGACCCCACAATACCATCGGCGCTACGGCggtaca                                       >  2:299654/1‑145 (MQ=255)
                                                                                                            gCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCTACGGCGTTTCa                                       >  2:646464/1‑150 (MQ=255)
                                                                                                            gCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                       >  1:512233/1‑150 (MQ=255)
                                                                                                               gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCTACGGCGTTTCACtt                                    >  2:1502903/1‑150 (MQ=255)
                                                                                                               gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACtt                                    >  1:514098/1‑150 (MQ=255)
                                                                                                                 tACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCACGCATGGGGAGACCCCCCACTACCATCGGCGCTACGGCGTTTCCCTTCt                                  >  1:830412/1‑150 (MQ=255)
                                                                                                                 tACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCACCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCACTACCATCGGCGCTACGGCGTTTCACTTCt                                  >  2:1754292/1‑150 (MQ=255)
                                                                                                                  aCCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACAATACCATCGGCGCTACGGCGTTTCACTTCTg                                 >  1:824860/1‑150 (MQ=255)
                                                                                                                  aCCCAGAGTCGAACTGGGGACCTCACCCTTACAAAGGGTGCGCTCTACCAAATGAGCCATATCAGCACGATAAAATAGATGCCTGGCAGTTCCCTACTCTCGCATCGGGAGACCCCACACTACCATCGGCGCTACCGCGGAACACTTCTg                                 >  1:1628218/1‑150 (MQ=25)
                                                                                                                   cccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                >  1:1721330/1‑150 (MQ=255)
                                                                                                                   cccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa                                >  2:698528/1‑150 (MQ=255)
                                                                                                                    ccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTCCCTTCTGAg                               >  2:1334492/1‑150 (MQ=255)
                                                                                                                     cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                              <  2:847611/150‑1 (MQ=255)
                                                                                                                         gTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                          >  1:1179697/1‑150 (MQ=255)
                                                                                                                           cGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCa                        >  2:1447261/1‑150 (MQ=255)
                                                                                                                             aaCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTCTCCCTTCTGAGTTCGGCATg                      >  2:499559/1‑150 (MQ=255)
                                                                                                                               cTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATggg                    >  2:1202069/1‑150 (MQ=255)
                                                                                                                                 ggggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGt                  >  2:543933/1‑150 (MQ=255)
                                                                                                                                      gcgcACCCTTGGTAAGGGTGAGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCCCCGTCCCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAGGGGGTCAggg             >  2:1588344/4‑149 (MQ=18)
                                                                                                                                      cctcaccctcacCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt             >  1:1709497/1‑150 (MQ=255)
                                                                                                                                        tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg           <  2:491546/150‑1 (MQ=255)
                                                                                                                                         cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg          >  1:1437950/1‑150 (MQ=255)
                                                                                                                                               tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc    <  1:1859862/150‑1 (MQ=255)
                                                                                                                                                 ccAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc  >  1:836215/1‑150 (MQ=255)
                                                                                                                                                 ccAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc  >  2:2083639/1‑150 (MQ=255)
                                                                                                                                                 ccAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGCACACcgc  >  2:1144167/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGC  >  NC_000913/3423476‑3423770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑