Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,659,468 A→C 7.6% E79D (GAA→GAC) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,659,4680AC 7.6% 377.3 / 14.7 138E79D (GAA→GAC) ‡mdtEanaerobic multidrug efflux transporter, ArcA‑regulated
Reads supporting (aligned to +/- strand):  ref base A (62/64);  new base C (5/6);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 142 total aligned reads displayed.

CCGCCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCT  >  NC_000913/3659320‑3659617
                                                                                                                                                    |                                                                                                                                                     
ccgccATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGCGCGGTATTATCATTAAACTCAACTTTATCGAAg                                                                                                                                                      >  1:1342171/1‑150 (MQ=255)
   ccATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCg                                                                                                                                                   <  2:860327/150‑1 (MQ=255)
    cATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTTTTATCATTAAACGCAACTTTATCGAAGGCGa                                                                                                                                                  >  2:510194/1‑150 (MQ=255)
    cATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTACACGCAACTTTATCGAAGGCGa                                                                                                                                                  >  2:1109024/1‑150 (MQ=255)
    cATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGa                                                                                                                                                  >  2:932359/1‑150 (MQ=255)
      tGACGCCTGAGGTCTGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCAACAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATa                                                                                                                                                <  2:1838997/150‑1 (MQ=255)
      tGACGCCTGAGGGCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCGCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTAGTATCATTAAACGCAACTTTATCGAAGGCGATa                                                                                                                                                <  1:112703/150‑1 (MQ=255)
       gACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATaa                                                                                                                                               >  2:581845/1‑150 (MQ=255)
        aCGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCATCTTTATCGAAGGCGATaaa                                                                                                                                              >  1:102701/1‑150 (MQ=255)
          gCCTGAGCGCGGTGTCGGCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGt                                                                                                                                            <  1:336520/150‑1 (MQ=255)
           ccTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGGCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGtt                                                                                                                                           <  2:1683164/150‑2 (MQ=255)
            cTGAGGTCGGTGTCGTCACACTCTCCCCGGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATTCGTCCCCAGGTTGTCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGttg                                                                                                                                          <  2:900714/150‑3 (MQ=255)
            cTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGttg                                                                                                                                          <  1:2043069/150‑3 (MQ=255)
             ggAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGttgc                                                                                                                                         <  2:1773773/149‑4 (MQ=255)
               aGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAAcc                                                                                                                                       <  2:454101/150‑1 (MQ=255)
                ggTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCa                                                                                                                                      <  2:1098868/150‑1 (MQ=255)
                    ggTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGc                                                                                                                                  <  1:1097023/150‑1 (MQ=255)
                      tgtCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGa                                                                                                                                >  2:905463/1‑150 (MQ=255)
                           tcACACTCTCCCCCGGGTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGACGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGc                                                                                                                           <  2:770491/150‑1 (MQ=255)
                            cacaCTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCt                                                                                                                          <  1:1631412/150‑1 (MQ=255)
                              cacTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGt                                                                                                                        <  1:100463/150‑1 (MQ=255)
                               acTCTCGCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCAGGTAGAACCGTTCCTTATGAAGTTGTCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                       <  2:1950890/150‑1 (MQ=255)
                               acTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                       >  1:1153155/1‑150 (MQ=255)
                               acTCTCCCCCGGTGCGGGCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                       <  1:543443/150‑1 (MQ=255)
                                ctctCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTAt                                                                                                                      >  1:1476023/1‑150 (MQ=255)
                                 tctcCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATc                                                                                                                     >  1:2049035/1‑150 (MQ=255)
                                  ctcCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGGCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCa                                                                                                                    <  1:1748552/150‑1 (MQ=255)
                                    cccccGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGa                                                                                                                  >  1:1809126/1‑150 (MQ=255)
                                        cGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGa                                                                                                              <  1:1498746/150‑1 (MQ=255)
                                         ggTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGAt                                                                                                             <  2:2036423/150‑1 (MQ=255)
                                           ttCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATcc                                                                                                           <  1:522306/150‑1 (MQ=255)
                                            tCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCt                                                                                                          <  1:1632470/150‑1 (MQ=255)
                                              ggTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGc                                                                                                        >  2:1458065/1‑150 (MQ=255)
                                                      ggtTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTAc                                                                                                <  2:1657317/149‑1 (MQ=255)
                                                       gtTGAGCGAATTGCCCGGTAGACCCGTTCCTGAAGAAGTTGCCAAGATACGTACCCAGGTGGGAGGTATTATCATTAAAAGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCAGCTTTACa                                                                                               >  2:308396/1‑150 (MQ=255)
                                                        ttGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAg                                                                                              >  1:1641803/1‑150 (MQ=255)
                                                        ttGAGCGAATTACCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAg                                                                                              <  1:1478562/150‑1 (MQ=255)
                                                          gAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGc                                                                                            >  1:1260771/1‑150 (MQ=255)
                                                            gCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCg                                                                                          >  2:1573719/1‑150 (MQ=255)
                                                            gCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCg                                                                                          <  1:1910989/150‑1 (MQ=255)
                                                            gCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCg                                                                                          >  2:2019193/1‑150 (MQ=255)
                                                             cGAATTGCCCGTTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCAAGGGGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGa                                                                                         <  2:391835/150‑1 (MQ=255)
                                                                 ttGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTa                                                                                     <  1:1800859/150‑1 (MQ=255)
                                                                   gCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTaaa                                                                                   >  1:531955/1‑150 (MQ=255)
                                                                   gCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTaaa                                                                                   >  1:766040/1‑150 (MQ=255)
                                                                    cccTGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCGAGGTGGGCGGTTTTATCATTAAACTCAACTTTTTCGAAGGCGTTAAATTGAACCAGGGGGATTCTCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAAc                                                                                  <  1:1525718/150‑1 (MQ=255)
                                                                       ggTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTcc                                                                               >  1:1594390/1‑150 (MQ=255)
                                                                             aCCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCaaa                                                                         >  2:1420369/1‑150 (MQ=255)
                                                                                gTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGc                                                                      >  1:2092416/1‑150 (MQ=255)
                                                                                 ttCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCt                                                                     <  2:696905/150‑1 (MQ=255)
                                                                                    cTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGc                                                                  >  1:1446563/1‑150 (MQ=255)
                                                                                    cTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGc                                                                  >  1:1832818/1‑150 (MQ=255)
                                                                                       aTGAGGTTGCCGAGATACGTCCCCAGGTGTGCTGTATTATCATTAAACGCAACTTTATCGAAGGAGATAAAGTGAAGCAGGTCGAGTCGCTGTATCAGATTGATCCTGCACCTTTTCAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTgg                                                               <  1:343168/150‑1 (MQ=255)
                                                                                        tGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTAGCTGGc                                                              >  1:184588/1‑150 (MQ=255)
                                                                                        tGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAAACAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGATCGCTGGc                                                              >  1:20817/1‑150 (MQ=255)
                                                                                         gAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCg                                                             <  2:48617/150‑1 (MQ=255)
                                                                                         gAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCg                                                             <  1:1015960/150‑1 (MQ=255)
                                                                                           aGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCTGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGaa                                                           <  2:1945812/150‑1 (MQ=255)
                                                                                           aGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGaa                                                           >  2:678492/1‑150 (MQ=255)
                                                                                             ttGCCGTGATACGTCGCCAGGTGGGCGGTTTTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACGTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAg                                                         <  2:380625/150‑1 (MQ=255)
                                                                                              tGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAgc                                                        <  2:1317015/150‑1 (MQ=255)
                                                                                               gCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAgcg                                                       >  2:2102184/1‑150 (MQ=255)
                                                                                                 cGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGCGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGct                                                     >  2:544004/1‑150 (MQ=255)
                                                                                                 cGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGct                                                     >  1:952158/1‑150 (MQ=255)
                                                                                                  gagaTACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctc                                                    <  1:1950745/150‑1 (MQ=255)
                                                                                                    gaTACGGCCCCAGGTGGGCGGTATTATCATTAACCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctctc                                                  <  2:687885/150‑1 (MQ=255)
                                                                                                      tACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTa                                                >  1:1217977/1‑150 (MQ=255)
                                                                                                      tACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTa                                                >  2:1429867/1‑150 (MQ=255)
                                                                                                         gTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCg                                             <  2:132020/150‑1 (MQ=255)
                                                                                                          tCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGc                                            >  2:803120/1‑150 (MQ=255)
                                                                                                           ccccAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGcc                                           >  2:793652/1‑150 (MQ=255)
                                                                                                              cAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGc                                        <  1:78694/150‑1 (MQ=255)
                                                                                                              cAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGc                                        <  1:1420613/150‑1 (MQ=255)
                                                                                                                  tGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATg                                    <  1:1645232/150‑1 (MQ=255)
                                                                                                                    ggCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGcc                                  >  2:1472821/1‑150 (MQ=255)
                                                                                                                     gCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTTGCGAAAGCGCTCTCTACCGCCAGCAATGccc                                 >  1:994638/1‑150 (MQ=255)
                                                                                                                      cGGTATTATCATTAAACTCAACTTTATCGATGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCg                                <  1:550138/150‑1 (MQ=255)
                                                                                                                            tatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAc                          >  2:1651319/1‑150 (MQ=255)
                                                                                                                             atCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAcc                         <  2:2067810/150‑1 (MQ=255)
                                                                                                                               cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCTGATTGATCCTGCACCTTTACAGGCCGCGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                       >  2:1274112/1‑150 (MQ=255)
                                                                                                                               cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                       <  1:2053459/150‑1 (MQ=255)
                                                                                                                               cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                       >  2:1748127/1‑150 (MQ=255)
                                                                                                                               cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                       <  2:1124890/150‑1 (MQ=255)
                                                                                                                                aTTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCttt                      >  2:1469898/1‑150 (MQ=255)
                                                                                                                                   aaaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCTATTCGCTGTATCAGATTGATCCTGCACCTTTAAAGGCCGAGCAAAACTCCGCCAAACGCTCGATGGCGAAAGCGCGCTCTACCGCCAGCAATGCCCGCATCACCTTTacc                   >  2:1241795/1‑148 (MQ=255)
                                                                                                                                   aaaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAc                   >  2:954272/1‑150 (MQ=255)
                                                                                                                                    tggttcaCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTTCAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                  >  2:82550/7‑150 (MQ=255)
                                                                                                                                    tggttcaCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAACGGCTCGCTGGCGAACGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                  >  2:1085460/7‑150 (MQ=255)
                                                                                                                                    aaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                  <  2:2073936/150‑1 (MQ=255)
                                                                                                                                     aCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCAGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCg                 >  1:729460/1‑150 (MQ=255)
                                                                                                                                       gCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGcc               <  2:2077651/150‑1 (MQ=255)
                                                                                                                                        cAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGGGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCa              >  2:1207817/1‑150 (MQ=255)
                                                                                                                                        cAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCa              >  1:1738247/1‑150 (MQ=255)
                                                                                                                                          aCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATACTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCCGCAATGCCCGCATCACCTTTACCCGCCAgg            >  1:1847976/1‑150 (MQ=255)
                                                                                                                                             ttATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAAAGCCCGCATCACCTTTACCCGCCCGGCAt         >  2:1266117/1‑150 (MQ=255)
                                                                                                                                              tATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGAATAAACCTGCACCTTTACAGGCCGAGCTGAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTCCCGCCACAAATGCCCGCCTCACCTTTAACCGCCAGGCATc        >  1:109139/1‑150 (MQ=255)
                                                                                                                                               aTCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCg       >  1:1482547/1‑150 (MQ=255)
                                                                                                                                                  gAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTg    >  1:470673/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGAAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGc   >  1:412374/1‑150 (MQ=255)
                                                                                                                                                    aGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCATAACAGGCCTAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCt  >  1:36726/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCGCCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCT  >  NC_000913/3659320‑3659617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: