Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,659,470 G→T 7.1% G80V (GGC→GTC) ‡ mdtE → anaerobic multidrug efflux transporter, ArcA‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,659,4700GT 7.1% 413.2 / 17.1 141G80V (GGC→GTC) ‡mdtEanaerobic multidrug efflux transporter, ArcA‑regulated
Reads supporting (aligned to +/- strand):  ref base G (64/67);  new base T (5/5);  total (69/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

Only 100 of 144 total aligned reads displayed.

CCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGA  >  NC_000913/3659323‑3659619
                                                                                                                                                   |                                                                                                                                                     
ccATTACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTAGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCTTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGGGGGCGGTATTATCATTAACCGCAACTTTATCGAAGGCg                                                                                                                                                     >  2:1481484/1‑150 (MQ=255)
ccATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCg                                                                                                                                                     <  2:860327/150‑1 (MQ=255)
 cATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTACACGCAACTTTATCGAAGGCGa                                                                                                                                                    >  2:1109024/1‑150 (MQ=255)
 cATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGa                                                                                                                                                    >  2:932359/1‑150 (MQ=255)
   tGACGCCTGAGGTCTGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCAACAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATa                                                                                                                                                  <  2:1838997/150‑1 (MQ=255)
   tGACGCCTGAGGGCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCGCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTAGTATCATTAAACGCAACTTTATCGAAGGCGATa                                                                                                                                                  <  1:112703/150‑1 (MQ=255)
     aCGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCATCTTTATCGAAGGCGATaaa                                                                                                                                                >  1:102701/1‑150 (MQ=255)
       gCCTGAGCGCGGTGTCGGCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGt                                                                                                                                              <  1:336520/150‑1 (MQ=255)
        ccTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTg                                                                                                                                             >  1:1229554/1‑150 (MQ=255)
        ccTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGGCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGtt                                                                                                                                             <  2:1683164/150‑2 (MQ=255)
         cTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGCCTTCGATAAAGttg                                                                                                                                            <  1:2043069/150‑3 (MQ=255)
                cGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAggg                                                                                                                                     >  2:2019159/1‑150 (MQ=255)
                 ggTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGc                                                                                                                                    <  1:1097023/150‑1 (MQ=255)
                   tgtCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGa                                                                                                                                  >  2:905463/1‑150 (MQ=255)
                        tcACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCga                                                                                                                             >  2:1451728/1‑149 (MQ=255)
                         cacaCTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCt                                                                                                                            <  1:1631412/150‑1 (MQ=255)
                           cacTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGt                                                                                                                          <  1:100463/150‑1 (MQ=255)
                            acTCTCGCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCAGGTAGAACCGTTCCTTATGAAGTTGTCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                         <  2:1950890/150‑1 (MQ=255)
                            acTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                         >  1:1153155/1‑150 (MQ=255)
                            acTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                         <  2:1083605/150‑1 (MQ=255)
                            acTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGCAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                         <  2:1312750/150‑1 (MQ=255)
                            acTCTCCCCCGGTGCGGGCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTa                                                                                                                         <  1:543443/150‑1 (MQ=255)
                              tctcCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATc                                                                                                                       >  1:2049035/1‑150 (MQ=255)
                               ctcCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCa                                                                                                                      >  1:48617/1‑150 (MQ=255)
                               ctcCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGGCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCa                                                                                                                      <  1:1748552/150‑1 (MQ=255)
                                 cccccGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGa                                                                                                                    >  1:1809126/1‑150 (MQ=255)
                                     cGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGa                                                                                                                <  1:1498746/150‑1 (MQ=255)
                                      ggTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGTTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGAt                                                                                                               <  1:1473368/150‑1 (MQ=255)
                                      ggTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGAt                                                                                                               <  2:2036423/150‑1 (MQ=255)
                                        ttCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATcc                                                                                                             <  1:522306/150‑1 (MQ=255)
                                         tCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCt                                                                                                            <  1:1632470/150‑1 (MQ=255)
                                           ggTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGc                                                                                                          >  2:1458065/1‑150 (MQ=255)
                                            gTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCa                                                                                                         <  2:798776/150‑1 (MQ=255)
                                            gTCAATGTGTGGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATGCGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGACGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCa                                                                                                         <  1:755591/150‑1 (MQ=255)
                                              cAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCAcc                                                                                                       <  2:467660/150‑1 (MQ=255)
                                              cAATGTGTGGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCAcc                                                                                                       <  2:541252/150‑1 (MQ=255)
                                               aaTGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCt                                                                                                      >  1:2073936/1‑150 (MQ=255)
                                                    gtTGAGCGAATTGCCCGGTAGACCCGTTCCTGAAGAAGTTGCCAAGATACGTACCCAGGTGGGAGGTATTATCATTAAAAGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCAGCTTTACa                                                                                                 >  2:308396/1‑150 (MQ=255)
                                                     ttGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAg                                                                                                >  1:1641803/1‑150 (MQ=255)
                                                     ttGAGCGAATTACCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAg                                                                                                <  1:1478562/150‑1 (MQ=255)
                                                      tGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAgg                                                                                               >  2:1891874/1‑150 (MQ=255)
                                                       gAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGc                                                                                              >  1:1260771/1‑150 (MQ=255)
                                                         gCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCg                                                                                            <  1:1910989/150‑1 (MQ=255)
                                                          cGAATTGCCCGTTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCAAGGGGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGa                                                                                           <  2:391835/150‑1 (MQ=255)
                                                             aTTGCCCGGTAGAACCGTTCGTTTTGAAGGTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTTTCGAAGGCGATAAAGTGAACCAGTGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCt                                                                                        <  2:1329091/150‑1 (MQ=255)
                                                              ttGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTa                                                                                       <  1:1800859/150‑1 (MQ=255)
                                                              ttGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGAACCTTTACAGGCCGAGCTa                                                                                       >  1:1347111/1‑150 (MQ=255)
                                                                gCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTaaa                                                                                     >  1:766040/1‑150 (MQ=255)
                                                                 cccTGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCGAGGTGGGCGGTTTTATCATTAAACTCAACTTTTTCGAAGGCGTTAAATTGAACCAGGGGGATTCTCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAAc                                                                                    <  1:1525718/150‑1 (MQ=255)
                                                                    ggTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTcc                                                                                 >  1:1594390/1‑150 (MQ=255)
                                                                     gTAGAACCGGTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCCGCACCTTTACAGGCCGAGCTAAACTCCg                                                                                <  2:71716/150‑1 (MQ=255)
                                                                      tAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATATAGTGAACCAGGGCGCTTCGGTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAACCTCCGc                                                                               >  1:53372/1‑150 (MQ=255)
                                                                       aGACCCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTTTTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGcc                                                                              <  2:1222470/150‑1 (MQ=255)
                                                                          aCCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCaaa                                                                           >  2:1420369/1‑150 (MQ=255)
                                                                             gTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGc                                                                        >  1:2092416/1‑150 (MQ=255)
                                                                              ttCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCt                                                                       <  2:696905/150‑1 (MQ=255)
                                                                                 cTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGc                                                                    >  1:1446563/1‑150 (MQ=255)
                                                                                  ttATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCt                                                                   <  1:184487/150‑1 (MQ=255)
                                                                                    aTGAGGTTGCCGAGATACGTCCCCAGGTGTGCTGTATTATCATTAAACGCAACTTTATCGAAGGAGATAAAGTGAAGCAGGTCGAGTCGCTGTATCAGATTGATCCTGCACCTTTTCAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTgg                                                                 <  1:343168/150‑1 (MQ=255)
                                                                                     tGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTAGCTGGc                                                                >  1:184588/1‑150 (MQ=255)
                                                                                     tGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAAACAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGATCGCTGGc                                                                >  1:20817/1‑150 (MQ=255)
                                                                                      gAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCg                                                               <  2:48617/150‑1 (MQ=255)
                                                                                      gAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCg                                                               <  1:1015960/150‑1 (MQ=255)
                                                                                        aGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGaa                                                             >  2:678492/1‑150 (MQ=255)
                                                                                          ttGCCGTGATACGTCGCCAGGTGGGCGGTTTTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACGTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAg                                                           <  2:380625/150‑1 (MQ=255)
                                                                                            gCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAgcg                                                         >  2:2102184/1‑150 (MQ=255)
                                                                                              cGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGCGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGct                                                       >  2:544004/1‑150 (MQ=255)
                                                                                              cGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGct                                                       >  1:952158/1‑150 (MQ=255)
                                                                                               gagaTACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctc                                                      <  1:1950745/150‑1 (MQ=255)
                                                                                                agaTACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGctct                                                     <  1:888474/150‑1 (MQ=255)
                                                                                                      gTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCg                                               <  2:132020/150‑1 (MQ=255)
                                                                                                       tCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGc                                              >  2:803120/1‑150 (MQ=255)
                                                                                                        ccccAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGcc                                             <  2:912102/150‑1 (MQ=255)
                                                                                                        ccccAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGcc                                             >  2:793652/1‑150 (MQ=255)
                                                                                                         cccAGGGGGGCGGTATTATCATTAAACGCAACATTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCa                                            <  2:175740/150‑1 (MQ=255)
                                                                                                               tGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATg                                      <  1:1645232/150‑1 (MQ=255)
                                                                                                                 ggCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGcc                                    >  2:1472821/1‑150 (MQ=255)
                                                                                                                   cGGTATTATCATTAAACTCAACTTTATCGATGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCg                                  <  1:550138/150‑1 (MQ=255)
                                                                                                                         tatCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAc                            >  2:1651319/1‑150 (MQ=255)
                                                                                                                          atCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCAcc                           <  2:2067810/150‑1 (MQ=255)
                                                                                                                            cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCTGATTGATCCTGCACCTTTACAGGCCGCGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                         >  2:1274112/1‑150 (MQ=255)
                                                                                                                            cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                         >  2:1748127/1‑150 (MQ=255)
                                                                                                                            cATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCtt                         <  2:1124890/150‑1 (MQ=255)
                                                                                                                              ttAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATGCGCGGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTa                       <  1:391606/150‑1 (MQ=255)
                                                                                                                                aaaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCTATTCGCTGTATCAGATTGATCCTGCACCTTTAAAGGCCGAGCAAAACTCCGCCAAACGCTCGATGGCGAAAGCGCGCTCTACCGCCAGCAATGCCCGCATCACCTTTacc                     >  2:1241795/1‑148 (MQ=255)
                                                                                                                                aaaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAc                     >  2:954272/1‑150 (MQ=255)
                                                                                                                                 tggttcaCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAACGGCTCGCTGGCGAACGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                    >  2:1085460/7‑150 (MQ=255)
                                                                                                                                 aaCGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAcc                    <  2:2073936/150‑1 (MQ=255)
                                                                                                                                  ggttcaCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCg                   >  2:681102/6‑150 (MQ=255)
                                                                                                                                    gCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGcc                 <  2:2077651/150‑1 (MQ=255)
                                                                                                                                     tcaCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGTCCGACCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAAACGCCa                >  2:878374/3‑150 (MQ=255)
                                                                                                                                     cAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCa                >  1:1738247/1‑150 (MQ=255)
                                                                                                                                       aCTTTATCGCCTTCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATACTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCCGCAATGCCCGCATCACCTTTACCCGCCAgg              >  1:1847976/1‑150 (MQ=255)
                                                                                                                                           tATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGAATAAACCTGCACCTTTACAGGCCGAGCTGAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTCCCGCCACAAATGCCCGCCTCACCTTTAACCGCCAGGCATc          >  1:109139/1‑150 (MQ=255)
                                                                                                                                            aTCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCg         >  1:1482547/1‑150 (MQ=255)
                                                                                                                                               gAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTg      >  2:1654405/1‑150 (MQ=255)
                                                                                                                                                aaGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGAAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGc     >  1:412374/1‑150 (MQ=255)
                                                                                                                                                 aGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATACTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCt    >  2:1111659/1‑150 (MQ=255)
                                                                                                                                                  ggCGATAAAGTGAACCAGGGCGATTCGCGGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTg   <  2:2075489/150‑1 (MQ=255)
                                                                                                                                                   gCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTACCCGCCAGGCATCGTTGCTGa  >  2:491662/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCATGACGCCTGAGGTCGGTGTCGTCACACTCTCCCCCGGTTCGGTCAATGTGTTGAGCGAATTGCCCGGTAGAACCGTTCCTTATGAAGTTGCCGAGATACGTCCCCAGGTGGGCGGTATTATCATTAAACGCAACTTTATCGAAGGCGATAAAGTGAACCAGGGCGATTCGCTGTATCAGATTGATCCTGCACCTTTACAGGCCGAGCTAAACTCCGCCAAAGGCTCGCTGGCGAAAGCGCTCTCTACCGCCAGCAATGCCCGCATCACCTTTAACCGCCAGGCATCGTTGCTGA  >  NC_000913/3659323‑3659619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: