Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,150 C→T Q82* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,1500CT99.1% 367.7 / NA 111Q82* (CAG→TAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (48/61);  minor base A (0/1);  total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC  >  NC_000913/756004‑756294
                                                                                                                                                  |                                                                                                                                                
gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAgg                                                                                                                                               <  2:196124/150‑1 (MQ=255)
  gcgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGAc                                                                                                                                             <  1:142988/150‑1 (MQ=255)
       gctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAt                                                                                                                                        >  2:623658/1‑150 (MQ=255)
        ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAtt                                                                                                                                       <  2:1856223/150‑1 (MQ=255)
         tctatAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACACATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTg                                                                                                                                      <  2:151078/150‑1 (MQ=255)
         gctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTg                                                                                                                                      <  2:1822213/149‑1 (MQ=255)
            ctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAat                                                                                                                                   <  2:675735/150‑1 (MQ=255)
            atAAAGTCTTCCCGACCCGTTCCCATACCATTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAat                                                                                                                                   <  1:668212/149‑1 (MQ=255)
              aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatat                                                                                                                                 <  2:1650413/150‑1 (MQ=255)
               aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGATCGGACTATATCGGTGACTAGGCCGCGATTGAatata                                                                                                                                >  2:1789454/1‑150 (MQ=255)
                 gTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtg                                                                                                                              >  1:1889853/1‑150 (MQ=255)
                 gTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCAGTACCGTGGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGACAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtg                                                                                                                              <  1:1328488/150‑1 (MQ=255)
                   cTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtgtg                                                                                                                            <  1:737609/150‑1 (MQ=255)
                     tCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTa                                                                                                                          <  2:1633810/150‑1 (MQ=255)
                      cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTaa                                                                                                                         >  2:1397630/1‑150 (MQ=255)
                      cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTaa                                                                                                                         >  2:838487/1‑150 (MQ=255)
                      cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAGTGAATATATGTGTaa                                                                                                                         >  2:1389714/1‑150 (MQ=255)
                           cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCg                                                                                                                    >  2:342617/1‑150 (MQ=255)
                            ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCgg                                                                                                                   >  1:1950496/1‑150 (MQ=255)
                              gTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                                                 >  1:129817/1‑150 (MQ=255)
                                tCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCg                                                                                                               >  1:151089/1‑150 (MQ=255)
                                   cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                                            >  2:1003258/1‑150 (MQ=255)
                                     tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                          <  2:1220203/150‑1 (MQ=255)
                                     tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                          <  1:999365/150‑1 (MQ=255)
                                      aCCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTTGGTAATACCCATGAAGATAACTTGGAATGGAATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCg                                                                                                         <  2:359838/150‑1 (MQ=255)
                                       ccGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                                        >  2:582320/1‑150 (MQ=255)
                                       ccGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                                        <  1:1511896/150‑1 (MQ=255)
                                        cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt                                                                                                       <  2:725274/150‑1 (MQ=255)
                                         gTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATATCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                                                                      >  1:1132351/1‑150 (MQ=255)
                                         gTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                                                                      >  1:1778290/1‑150 (MQ=255)
                                             cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTgg                                                                                                  <  2:533149/150‑1 (MQ=255)
                                             cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTgg                                                                                                  <  1:184261/150‑1 (MQ=255)
                                             cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTgg                                                                                                  <  2:1499730/150‑1 (MQ=255)
                                              tGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGa                                                                                                 >  1:1039179/1‑150 (MQ=255)
                                                cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                               >  1:1394128/1‑150 (MQ=255)
                                                cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAATGAATATATGTGTAACACCGGGCCGGAAGCGATTCTGGAAc                                                                                               >  2:1232109/1‑150 (MQ=255)
                                                  cAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATTGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTc                                                                                             <  1:1347987/150‑1 (MQ=255)
                                                  cAACGCGGCAGTCCCGTTGCGCGGCGTAAAACCCAGGAAAATAACTCTGAAACGCATTTTTACTACAGCCTGAAAGGGTCGGACTATATCGGTGACTAGGCCGCGATGCAAAAAATGGGTACAAGCGGGCCGGCAGCGATTCTGGAACTc                                                                                             <  2:329419/150‑1 (MQ=255)
                                                    aGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa                                                                                           <  2:569209/150‑1 (MQ=255)
                                                      gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                                                                         <  2:1566542/150‑1 (MQ=255)
                                                       cggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAaca                                                                                        <  1:560496/150‑1 (MQ=255)
                                                          cATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATAGAATATATGTGTAAAAACGGGCCGGAAGCGATTCTGGAACTCGAACACAt                                                                                     >  1:176384/1‑150 (MQ=255)
                                                             tACCGTTTAGCTGTTTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTATGACGCGATTGAATATTTGTGTATCTCCGGGCCGGCAGCGATTCTGGAACTCGATCACATggg                                                                                  <  2:1580257/150‑1 (MQ=255)
                                                             tACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATTTGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAATCTGGAACTCGCGCACATggg                                                                                  >  2:468789/1‑150 (MQ=255)
                                                               ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc                                                                                <  1:1531967/150‑1 (MQ=255)
                                                               ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGAGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAATCTGGAACTCGAACACATGGGcc                                                                                >  1:770902/1‑150 (MQ=255)
                                                                 gTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGACCACATGGGCCTg                                                                              >  2:788796/1‑150 (MQ=255)
                                                                   tGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                                            <  1:1883594/150‑1 (MQ=255)
                                                                    gcgcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAGTCTGGAACTCGAACACATGGGCCTGCCg                                                                           <  2:1248563/150‑1 (MQ=255)
                                                                            tAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg                                                                   >  1:1738552/1‑150 (MQ=255)
                                                                             aaTACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                                                  >  1:1934202/1‑150 (MQ=255)
                                                                                      gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatgat                                                         >  2:1186707/1‑150 (MQ=255)
                                                                                      gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatgat                                                         <  1:717456/150‑1 (MQ=255)
                                                                                        aGATAACTGGGAATGTCATATGTACGACACCGTGATAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATTTGTGTAACACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg                                                       <  2:371457/150‑1 (MQ=255)
                                                                                             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGTCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                  >  1:1297459/1‑150 (MQ=255)
                                                                                             aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                  <  2:1925972/150‑1 (MQ=255)
                                                                                              cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtat                                                 >  1:33739/1‑150 (MQ=255)
                                                                                              cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGgat                                                 >  2:1778662/1‑150 (MQ=255)
                                                                                                gggAATGGCATATGTACGACACCGTGAAAGGGTCGGATTATATCGGTGCCTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatca                                               >  2:821985/1‑149 (MQ=255)
                                                                                                gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct                                               >  1:461618/1‑150 (MQ=255)
                                                                                                  gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                                             <  2:1519527/150‑1 (MQ=255)
                                                                                                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                         >  2:1378858/1‑150 (MQ=255)
                                                                                                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                         >  2:253635/1‑150 (MQ=255)
                                                                                                      ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                         >  2:1884763/1‑150 (MQ=255)
                                                                                                       gCATATGTACGACAGCGTGAAAGGGTCGGACTATATCGGTCACAAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTTGAACTCGAACACCTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                        <  2:113289/150‑1 (MQ=255)
                                                                                                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                      <  1:692683/150‑1 (MQ=255)
                                                                                                         atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                      <  1:706323/150‑1 (MQ=255)
                                                                                                           atGGACGACACCGTGCAAGGGTCTGACAATAGCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCTCGTCTCGATGATGGTCGTATCTATCAACGTCCg                                    <  2:138315/150‑1 (MQ=255)
                                                                                                              tACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                 <  1:1557269/150‑1 (MQ=255)
                                                                                                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg                                >  2:1728978/1‑150 (MQ=255)
                                                                                                               aCGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGACCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg                                <  2:129817/150‑1 (MQ=255)
                                                                                                               aCGACACCGGGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATAGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGATCTCGAACACATGGGCCTGCCGTTCTCGCCTCTCGATGATGGACGTATCTATCAACGAACGTTTg                                >  1:320882/1‑150 (MQ=255)
                                                                                                                cGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACTCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg                               <  1:1045141/150‑1 (MQ=255)
                                                                                                                cGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg                               <  1:623658/150‑1 (MQ=255)
                                                                                                                cGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg                               >  1:631615/1‑150 (MQ=255)
                                                                                                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                              <  1:1174617/150‑1 (MQ=255)
                                                                                                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                              <  2:2006397/150‑1 (MQ=255)
                                                                                                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                              <  1:610251/150‑1 (MQ=255)
                                                                                                                 gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAATGAATATATGTGTAAAACCGGGCCGGAAGCGAGTCTGGAACTCGAACTCATGGGCGTGCCGTTCTCGCGTCTCGATGATGGTCGAATCTATCAACGTCCGTTTGGc                              <  1:342617/150‑1 (MQ=255)
                                                                                                                  acacCGTGAAAGGGTCGGACTACATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg                             <  2:687491/150‑1 (MQ=255)
                                                                                                                   cacCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACAGGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg                            <  1:1442276/150‑1 (MQ=255)
                                                                                                                     ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGATTATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACTCATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                          <  1:68916/150‑1 (MQ=255)
                                                                                                                     ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                          >  2:1910425/1‑150 (MQ=255)
                                                                                                                     ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCCTATCTATCAACGTCCGTTTGGCGGTc                          <  2:922681/150‑1 (MQ=255)
                                                                                                                     ccGTGAAAGGGTCGGACTATATCGGAGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGCTc                          >  1:728749/1‑150 (MQ=255)
                                                                                                                       gTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCat                        >  1:1239858/1‑149 (MQ=255)
                                                                                                                       gTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg                        <  2:1778290/150‑1 (MQ=255)
                                                                                                                             ggggCGGACAATATCGGTGACTAGGACGCGATTGAATAAAGGGGTCAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaa                  <  1:1923741/150‑1 (MQ=255)
                                                                                                                               gTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATTATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                >  2:892411/1‑150 (MQ=255)
                                                                                                                                  ggACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACtt             <  2:673684/150‑1 (MQ=255)
                                                                                                                                  ggACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACtt             >  1:1475438/1‑150 (MQ=255)
                                                                                                                                   gACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc            >  1:115487/1‑150 (MQ=255)
                                                                                                                                       atatCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg        <  2:2017342/150‑1 (MQ=255)
                                                                                                                                       atatCGCTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTGTGGAACTCGAAGACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg        <  2:1946595/150‑1 (MQ=255)
                                                                                                                                          tCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAGGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGGCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcg     <  1:769304/150‑1 (MQ=255)
                                                                                                                                          tCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcg     <  2:151089/150‑1 (MQ=255)
                                                                                                                                            ggTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAg   >  1:43266/1‑150 (MQ=255)
                                                                                                                                             gTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc  <  1:880436/150‑1 (MQ=255)
                                                                                                                                             gTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTACCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc  >  1:118988/1‑150 (MQ=255)
                                                                                                                                             gTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATGCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGc  <  2:2037429/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGC  >  NC_000913/756004‑756294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: