Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,477,549 G→A A27A (GCC→GCT dsdC ← dsd operon activator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,5490GA98.4% 412.7 / NA 122A27A (GCC→GCTdsdCdsd operon activator; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (63/57);  minor base T (1/1);  total (64/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTA  >  NC_000913/2477403‑2477694
                                                                                                                                                  |                                                                                                                                                 
 tCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAg                                                                                                                                               >  2:1150353/1‑150 (MQ=255)
  ccccTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTagcagc                                                                                                                                              <  2:1187698/150‑1 (MQ=255)
  ccccTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTagcagc                                                                                                                                              >  1:1719759/1‑150 (MQ=255)
     cTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCAc                                                                                                                                           <  2:1746704/150‑1 (MQ=255)
     cTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCAc                                                                                                                                           <  2:1325827/150‑1 (MQ=255)
     cTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCAc                                                                                                                                           <  1:566257/150‑1 (MQ=255)
     cTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCAc                                                                                                                                           <  1:1305276/150‑1 (MQ=255)
     cTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAAAGCCCAAATCTTCTTCCAGCTGATTGATACGGTGGCATACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCACGGCGAAGGACTGATGCCTAGCAGCCAc                                                                                                                                           >  1:1460038/1‑150 (MQ=255)
      tCGTGCGTTAATTCCTCTTTGCGATGGGAACGAACAAACAACTGACTGCCCAATTCTTCTTCCAGCTGATTGATACGCTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTTGCAGCCACt                                                                                                                                          <  2:868892/150‑1 (MQ=255)
        gTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTc                                                                                                                                        <  2:1953775/150‑1 (MQ=255)
        gTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTc                                                                                                                                        <  1:243199/150‑1 (MQ=255)
          gCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCaa                                                                                                                                      <  2:1967020/150‑1 (MQ=255)
          gCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCaa                                                                                                                                      >  2:1958527/1‑150 (MQ=255)
           cGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATAGATACGGTGACATACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCaaa                                                                                                                                     >  1:280859/1‑150 (MQ=255)
            gTTAAATCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCGCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCaaaa                                                                                                                                    <  2:955809/150‑1 (MQ=255)
             ttAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAg                                                                                                                                   <  1:1174865/150‑1 (MQ=255)
             ttAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAg                                                                                                                                   <  2:1684541/150‑1 (MQ=255)
             ttAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAg                                                                                                                                   >  1:661476/1‑150 (MQ=255)
               aaTTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTa                                                                                                                                 <  1:1671124/150‑1 (MQ=255)
               aaTTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTa                                                                                                                                 <  1:229700/150‑1 (MQ=255)
                aTTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTAt                                                                                                                                >  2:803295/1‑150 (MQ=255)
                  tCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGc                                                                                                                              <  2:1564472/150‑1 (MQ=255)
                   ccACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCa                                                                                                                             <  2:1314978/150‑1 (MQ=255)
                   ccACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCa                                                                                                                             >  1:231682/1‑150 (MQ=255)
                    cACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCTACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCAt                                                                                                                            >  2:1959225/1‑150 (MQ=255)
                     aCTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCAtt                                                                                                                           >  1:1514197/1‑150 (MQ=255)
                     aCTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCAtt                                                                                                                           >  1:424055/1‑150 (MQ=255)
                       tttGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATCCGGTGACTTACCTCACTGGGGCTCAGCGACAATTCCTATGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCAtttt                                                                                                                         <  1:824121/150‑1 (MQ=255)
                         tGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTg                                                                                                                       <  1:1950449/150‑1 (MQ=255)
                          gCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGa                                                                                                                      <  1:1678947/150‑1 (MQ=255)
                           cGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGAt                                                                                                                     >  1:898004/1‑150 (MQ=255)
                           cGATGGGAACGAACAAACAACTGAATGCCCAATTCATCTTCAAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAAATCCACAGCCGCCAGGGCGAAGGACTGATGCCTAGAAGCCACTTCAAAAGTATGCATTTTTGAt                                                                                                                     >  2:1696219/1‑150 (MQ=255)
                              tGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAAt                                                                                                                  <  1:1771981/150‑1 (MQ=255)
                               gggAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAAtt                                                                                                                 <  2:1789094/150‑1 (MQ=255)
                                ggAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTg                                                                                                                <  1:902584/150‑1 (MQ=255)
                                  aacgaacAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGcc                                                                                                              <  1:654748/150‑1 (MQ=255)
                                   acgaacAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGGGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCa                                                                                                             <  1:1244276/150‑1 (MQ=255)
                                          aacaacTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATATTTGCCAGCCGTTa                                                                                                      >  2:69605/1‑150 (MQ=255)
                                           acaacTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCTCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTaa                                                                                                     <  1:1070968/150‑1 (MQ=255)
                                           acaacTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTaa                                                                                                     >  2:474624/1‑150 (MQ=255)
                                                tGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGTAATGATGCCTAGCAGCCACTTCAAAAGTATGAATTTTTGATAATAGCCAGCCGTTAagcagc                                                                                                >  2:423476/1‑150 (MQ=255)
                                                   aTGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGa                                                                                             >  2:683271/1‑150 (MQ=255)
                                                      cccAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGAttt                                                                                          >  2:1131917/1‑150 (MQ=255)
                                                       ccAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTc                                                                                         >  2:720878/1‑150 (MQ=255)
                                                       ccAATTCTTCTTCCAGCTGATTGATACGGGGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGCTAAGCAGCCGATTTc                                                                                         <  1:1732465/150‑1 (MQ=255)
                                                        cAATTCTTCATCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGATTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCt                                                                                        >  2:8310/1‑150 (MQ=255)
                                                                cttcCAGCTGATTGATACGGTGACTTACAGCACTGTGGCTCAGCGACAATTCCTCTGCCGCAAGGGCGAAGGACTGATGCCTAGATGCCACTTCAAAAGTATGGATTTTTGATATTTGCCAGCCGTTTAGCAGCCGATTTCTTATTTCAc                                                                                >  2:1702320/1‑150 (MQ=255)
                                                                   ccAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTTGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCTGCCGTGCAGCAGCCGATTTCTTATTTCCCGaa                                                                             >  2:103245/1‑150 (MQ=255)
                                                                    cAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCAAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAg                                                                            >  2:1166070/1‑150 (MQ=255)
                                                                       cTGATTGATACGGTGACTTACCGCACTGCGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAgggg                                                                         >  2:1033064/1‑150 (MQ=255)
                                                                        tgattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGt                                                                        >  2:68040/1‑150 (MQ=255)
                                                                        tgattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAAATCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGt                                                                        <  1:1356382/150‑1 (MQ=255)
                                                                         gattgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGtt                                                                       >  2:527798/1‑150 (MQ=255)
                                                                            tgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCa                                                                    <  1:803295/150‑1 (MQ=255)
                                                                            tgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCa                                                                    <  1:704944/150‑1 (MQ=255)
                                                                            tgatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTAGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCa                                                                    <  2:1822558/150‑1 (MQ=255)
                                                                             gatACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCAt                                                                   >  2:267087/1‑150 (MQ=255)
                                                                                 cGGTGACTTACCGCACTGGGGCGCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATc                                                               <  2:365022/150‑1 (MQ=255)
                                                                                  ggTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTTATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCa                                                              <  1:1290194/150‑1 (MQ=255)
                                                                                  ggTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCa                                                              >  1:401789/1‑150 (MQ=255)
                                                                                  ggTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTAGTTCACGAAGGGGTTCCATAATCa                                                              >  2:28888/1‑150 (MQ=255)
                                                                                       cTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCa                                                         >  1:808248/1‑150 (MQ=255)
                                                                                        ttACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCCGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAt                                                        >  2:1815446/1‑150 (MQ=255)
                                                                                         tACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAtt                                                       >  2:1592991/1‑150 (MQ=255)
                                                                                         tACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAtt                                                       >  1:1552233/1‑150 (MQ=255)
                                                                                         aaCCGCACTGCGGCTCAGCGACAATTCCTCTGCCGCCATGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTACTTATTTCACGAAGGCGTTCCATAATCACCACAtt                                                       >  2:825074/2‑150 (MQ=255)
                                                                                           ccGCCCTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACGGATGCCTAGCAGCCACTTCAAAAGTAAGCATTTTTGATAATTGCCAGCCGGTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAtttt                                                     >  2:1382684/1‑150 (MQ=255)
                                                                                            cGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCAttttt                                                    <  1:114120/150‑1 (MQ=255)
                                                                                              cACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCg                                                  <  1:877311/150‑1 (MQ=255)
                                                                                                cTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCt                                                <  2:231682/150‑1 (MQ=255)
                                                                                                     gCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAgt                                           <  2:24371/150‑1 (MQ=255)
                                                                                                     gCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAgt                                           >  1:1301467/1‑150 (MQ=255)
                                                                                                     gCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTAAAgt                                           >  2:1130583/1‑150 (MQ=255)
                                                                                                      cTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATTCATTTTTGAGAATTGCCAGCCGTTAAGCAGACGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAgag                                          >  1:1035460/1‑148 (MQ=255)
                                                                                                         aGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTaa                                       <  2:553826/150‑1 (MQ=255)
                                                                                                           cGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAAATGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTATTCGCATAAGTGTaaaa                                     >  2:1701608/1‑150 (MQ=255)
                                                                                                            gACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGGTTAAGTGTaaata                                    >  2:115814/1‑148 (MQ=255)
                                                                                                              cAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTaaaaaaa                                  >  2:1589680/1‑150 (MQ=255)
                                                                                                               aaTTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAAt                                 >  1:1505710/1‑150 (MQ=255)
                                                                                                                 ttCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAg                               <  1:1150353/150‑1 (MQ=255)
                                                                                                                 ttCCTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAg                               >  1:1724266/1‑150 (MQ=255)
                                                                                                                   ccTCTGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATTATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCg                             <  2:132950/150‑1 (MQ=255)
                                                                                                                    ctctGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCgg                            >  2:417260/1‑150 (MQ=255)
                                                                                                                    ctctGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCgg                            >  2:874789/1‑150 (MQ=255)
                                                                                                                    ctctGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCgg                            <  1:658612/150‑1 (MQ=255)
                                                                                                                    ctctGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTACATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCgg                            >  1:333991/1‑150 (MQ=255)
                                                                                                                     tctGCCGCCAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCATCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGc                           <  1:1196126/150‑1 (MQ=255)
                                                                                                                            ccAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATATCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAAttt                    >  2:904318/1‑150 (MQ=255)
                                                                                                                             cAGGGCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTc                   <  1:1958527/150‑1 (MQ=255)
                                                                                                                               gggCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAg                 >  1:1233639/1‑150 (MQ=255)
                                                                                                                               gggCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAg                 <  2:615593/150‑1 (MQ=255)
                                                                                                                                 gCGAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCt               <  2:401789/150‑1 (MQ=255)
                                                                                                                                   gAAGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTat             <  2:424055/150‑1 (MQ=255)
                                                                                                                                    aaGGACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTAATTTCTCGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatg            <  1:1099859/150‑1 (MQ=255)
                                                                                                                                    aaGGACTGATGCCTAGCAGCCACTTAAAAAGTATGCATTTTTCATAATTGCCAGCCGTTAAGCCGCCGATTACTTATTTCACGATGGGGTTGCATAATCCGCTCATTGTTCGCTGAAGTGTAAAAATATAGCGGCAAAATTTGAGGTatg            >  2:814251/1‑150 (MQ=255)
                                                                                                                                      ggACTGATGCCTAGCAGCCACTTCAAAAGTATGGATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgag          <  2:1697592/150‑1 (MQ=255)
                                                                                                                                       gACTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgaga         <  1:874789/150‑1 (MQ=255)
                                                                                                                                        aCTGATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgagat        >  1:819723/1‑150 (MQ=255)
                                                                                                                                           gATGCCTAGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTatgagatgat     >  1:575955/1‑149 (MQ=255)
                                                                                                                                              cccTAGCAGCAAATTAAAAATTATGCATTTTTTATAATTGCCAGCCGTTAACCAGCCGATTTCTTATTTCACGATGGGGTTCCATAATCACATAATTTTTCGCTTAAGTGTAAAAAAATAGCGCAAAAATTTCAGCTATGAGATTAGCTa  <  2:1164147/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCAAAAGTATGCATTTTTGATAATTGCCAGCCGTTAAGCAGCCGATTTCTTATTTCACGAAGGGGTTCCATAATCACCTCATTTTTCGCTTAAGTGTAAAAAAATAGCGGCAAAATTTCAGCTATGAGATGAGCTA  >  NC_000913/2477403‑2477694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: