Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 127,605 A→C 5.2% intergenic (+18/‑307) aceF → / → lpd pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2/dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913127,6050AC 5.2% 1405.9 / 8.1 525intergenic (+18/‑307)aceF/lpdpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2/dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2‑oxoglutarate dehydrogenases complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (228/253);  new base C (11/29);  total (240/285)
Fisher's exact test for biased strand distribution p-value = 1.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 531 total aligned reads displayed.

AGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCA  >  NC_000913/127457‑127754
                                                                                                                                                    |                                                                                                                                                     
agTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAAc                                                                                                                                                      <  2:4363915/150‑1 (MQ=255)
        ccGCGTCTGCTGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCt                                                                                                                                              <  2:2820009/150‑1 (MQ=255)
        ccGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCt                                                                                                                                              >  1:7752492/1‑150 (MQ=255)
        ccGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGACTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCt                                                                                                                                              >  1:6236990/1‑150 (MQ=255)
              cTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTc                                                                                                                                        >  2:5761073/1‑150 (MQ=255)
               tgttgctgcCTTTTTCGCTGTCCTACGACCACGGCGTGATCGTCGGTGGTGATGGTGCCCGTTTCTTTACCATCATTAACTACACGCTGTCTGACCTTGGCCGTCTGGTGTTGTAAGTAAAAGTGCCGGCCCAACGGCCGGCTTTTTTCt                                                                                                                                       <  1:4906195/147‑1 (MQ=255)
                     tgcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAAt                                                                                                                                 >  1:3712915/1‑150 (MQ=255)
                     tgcCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAAt                                                                                                                                 <  2:2385246/150‑1 (MQ=255)
                       ccGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAAtct                                                                                                                               >  1:1819245/1‑150 (MQ=255)
                       ccGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAAtct                                                                                                                               <  1:5010815/150‑1 (MQ=255)
                       ccGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAAtct                                                                                                                               >  1:4431556/1‑150 (MQ=255)
                        cGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAAtctc                                                                                                                              >  2:8005648/1‑150 (MQ=255)
                          aTTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCAt                                                                                                                            >  1:6584673/1‑150 (MQ=255)
                                ctctcCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGt                                                                                                                      >  1:5134494/1‑150 (MQ=255)
                                 tctcCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACCCGCTGTCGGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTa                                                                                                                     <  1:1649966/150‑1 (MQ=255)
                                 tctcCTTCGAAAACCGCGTGATCGACGGTGCTGATGGTGCACGTTTCATTACCATCATTAACAACACGCAGTCTGAAATTCGCCGTCTGGTGTTGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTa                                                                                                                     >  1:6643447/1‑150 (MQ=255)
                                  ctcCTTCGACCACCGCGTGATCGACGGTGCAGATGTTGCCAGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCTTCTGGTGATGTAAGTAAAAGAGCCGGCACAACGGCCGGCTTTTTTCAGGTAATCTCATGAATGTAt                                                                                                                    >  2:3672936/1‑150 (MQ=255)
                                     cTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTATGGTAATCTCATGAATGTATTGa                                                                                                                 >  1:3944854/1‑150 (MQ=255)
                                      ttCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAg                                                                                                                <  1:1006386/150‑1 (MQ=255)
                                      ttCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAg                                                                                                                >  2:189797/1‑150 (MQ=255)
                                       tCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTATGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAgg                                                                                                               <  1:8113991/150‑1 (MQ=255)
                                          accaccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGtta                                                                                                            >  1:2338066/1‑150 (MQ=255)
                                            caccGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGttatt                                                                                                          <  1:3444262/150‑1 (MQ=255)
                                               cgcgTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTACCCACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGc                                                                                                       <  2:2202570/150‑1 (MQ=255)
                                               cgcgTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGc                                                                                                       <  2:7717184/150‑1 (MQ=255)
                                               cgcgTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGc                                                                                                       <  1:5985138/150‑1 (MQ=255)
                                                 cgTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGa                                                                                                     >  1:4038930/1‑150 (MQ=255)
                                                  gTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACACCACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGTCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGaa                                                                                                    <  2:1243330/150‑1 (MQ=255)
                                                    gATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATa                                                                                                  <  1:7644256/150‑1 (MQ=255)
                                                    gATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATa                                                                                                  >  1:484105/1‑150 (MQ=255)
                                                     aTCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAg                                                                                                 >  1:3543182/1‑150 (MQ=255)
                                                     aTCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAg                                                                                                 >  1:3164752/1‑150 (MQ=255)
                                                      tCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGa                                                                                                >  2:8075590/1‑150 (MQ=255)
                                                       cGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGAc                                                                                               <  1:6058159/150‑1 (MQ=255)
                                                         aCGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACaa                                                                                             <  1:5577152/150‑1 (MQ=255)
                                                         aCGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACaa                                                                                             <  1:3291247/150‑1 (MQ=255)
                                                          cGGTGCTGATGGTGCCCGTTTCATTACCATCAGTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACaaa                                                                                            <  2:4159153/150‑1 (MQ=255)
                                                           ggTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAAt                                                                                           <  2:2930572/150‑1 (MQ=255)
                                                            gTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATc                                                                                          >  1:7221806/1‑150 (MQ=255)
                                                               cTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGt                                                                                       <  2:3364743/150‑1 (MQ=255)
                                                                   tGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGcc                                                                                   <  1:1914541/150‑1 (MQ=255)
                                                                   tGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGcc                                                                                   >  2:4730767/1‑150 (MQ=255)
                                                                    ggTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCg                                                                                  >  1:4197205/1‑150 (MQ=255)
                                                                     gTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGt                                                                                 >  1:7422841/1‑150 (MQ=255)
                                                                          cGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTttgtt                                                                            >  2:1750453/1‑150 (MQ=255)
                                                                            tttCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTGTCTGGTAATCTCATGAATGTAGTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTGtgttgt                                                                          >  1:3793007/1‑150 (MQ=255)
                                                                                aTTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTaa                                                                      >  2:7432522/1‑150 (MQ=255)
                                                                                aTTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTaa                                                                      >  2:3000986/1‑150 (MQ=255)
                                                                                 tttccATCATTAACTACACGCTGTCTGAGATTCGCCTTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGGCGGCTTTTTTCTGGTAATCTCATGAATGTATTTAGGTTTTTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTaaa                                                                     <  2:5290280/147‑1 (MQ=255)
                                                                                    ccAACATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAt                                                                  <  2:8217057/150‑1 (MQ=255)
                                                                                     catcatTCACAACCCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAtt                                                                 <  2:6604937/150‑1 (MQ=255)
                                                                                     catcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAtt                                                                 >  1:6407349/1‑150 (MQ=255)
                                                                                     catcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAtt                                                                 <  1:818749/150‑1 (MQ=255)
                                                                                     catcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAAtt                                                                 <  2:7501129/150‑1 (MQ=255)
                                                                                      atcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTg                                                                >  2:5084258/1‑150 (MQ=255)
                                                                                      atcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTg                                                                >  2:1451745/1‑150 (MQ=255)
                                                                                       tcatTAACACCACGCTTTCTTACATTCTCCGTCTGGTTATGTAAGTAAAAGATCCGGCCGAACGGCCGGCTTTTTTCTTGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTTTTTAAAAATTGt                                                               <  1:1269120/150‑1 (MQ=255)
                                                                                       tcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGt                                                               <  1:5420949/150‑1 (MQ=255)
                                                                                       tcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGt                                                               <  1:4716245/150‑1 (MQ=255)
                                                                                       tcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGt                                                               >  2:6263952/1‑150 (MQ=255)
                                                                                       tcatTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGt                                                               >  2:6935521/1‑150 (MQ=255)
                                                                                        catTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGtt                                                              <  2:7291076/150‑1 (MQ=255)
                                                                                          ttAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTaa                                                            >  1:5834013/1‑150 (MQ=255)
                                                                                          ttAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTaa                                                            <  1:573527/150‑1 (MQ=255)
                                                                                             acaacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACaa                                                         >  1:5054772/1‑150 (MQ=255)
                                                                                             acaacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACaa                                                         <  1:3010170/150‑1 (MQ=255)
                                                                                             acaacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACaa                                                         <  2:4540809/150‑1 (MQ=255)
                                                                                             acaacaCGCTGTCTGACATTCGCCGTATGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACaa                                                         <  2:2095246/150‑1 (MQ=255)
                                                                                              caacaCGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAt                                                        >  1:2592581/1‑150 (MQ=255)
                                                                                                acacGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAttt                                                      <  2:5435705/150‑1 (MQ=255)
                                                                                                    gCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTa                                                  <  2:1008520/150‑1 (MQ=255)
                                                                                                          tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATAc                                            >  2:3031213/1‑150 (MQ=255)
                                                                                                          tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATAc                                            <  2:2579194/150‑1 (MQ=255)
                                                                                                          tGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATAc                                            >  2:6670769/1‑150 (MQ=255)
                                                                                                          tGACATTCCCCGTCTGGAGATGTAAGTAAAACACCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATAc                                            <  2:5662902/150‑1 (MQ=255)
                                                                                                             cATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGa                                         <  1:212052/150‑1 (MQ=255)
                                                                                                             cATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGa                                         <  2:1341133/150‑1 (MQ=255)
                                                                                                              aTTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGAc                                        >  2:7929899/1‑150 (MQ=255)
                                                                                                                  gCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTGTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTATAAATTGTTAACAATTTTGTTAAATACCGACGGAt                                    <  1:1440718/150‑1 (MQ=255)
                                                                                                                   ccGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAAATTTGTAAAATACCGACGGATa                                   >  2:5899152/1‑150 (MQ=255)
                                                                                                                      tCTGGTGATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGaa                                <  1:1998739/150‑1 (MQ=255)
                                                                                                                      tCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTTTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGaa                                <  1:6941007/150‑1 (MQ=255)
                                                                                                                        tGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGGTAACAATTTTGTAAAATACCGACGGATAGAACg                              >  2:265959/1‑150 (MQ=255)
                                                                                                                           tGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGAcc                           <  2:4962435/150‑1 (MQ=255)
                                                                                                                            gATGTAAGTAAAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGAccc                          <  2:6034904/150‑1 (MQ=255)
                                                                                                                              tGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCgg                        <  2:4900767/150‑1 (MQ=255)
                                                                                                                               gtaagtaaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggt                       >  2:3768573/1‑150 (MQ=255)
                                                                                                                               gtaagtaaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggt                       >  2:1397242/1‑150 (MQ=255)
                                                                                                                                taagtaaAAGAGCCGGCCCACCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAACTCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtg                      <  2:4038930/150‑1 (MQ=255)
                                                                                                                                 aagtaaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtgg                     <  2:900250/150‑1 (MQ=255)
                                                                                                                                  cagaaaaaaGCCGGCCGTTGGGCCGGCTCTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtggt                    >  2:6202925/4‑150 (MQ=255)
                                                                                                                                  agtaaAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTTTTAACAATTTTGTAAAATAACGACGGATAGAACGACCCggtggt                    >  1:2732903/1‑150 (MQ=255)
                                                                                                                                     aaaaGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCggtggtggt                 >  2:3894565/1‑150 (MQ=255)
                                                                                                                                       aaGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTa               <  2:6127725/150‑1 (MQ=255)
                                                                                                                                         gagCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAgg             <  2:7598982/150‑1 (MQ=255)
                                                                                                                                         gagCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAgg             <  2:3201246/150‑1 (MQ=255)
                                                                                                                                           cccGGCCCACCGCCGGGATTTTTTCTGGTAATCTCTTTATTTTTTTGTGTTTTTTAGCGAATAGACAATTCGGTTGCGTTTTTTTTTTTAAAAATTTTTAACATTTTTTTAAAATACCGCCGGATTGAACGACCCGGTGGTGGTTAGGGt           <  1:6882265/149‑1 (MQ=255)
                                                                                                                                             cGGCCCCCCGGCCGGCTTTTTTCTGGTAATCTCATGAAGGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTAt         <  1:4562145/150‑1 (MQ=255)
                                                                                                                                                   aaCGGCCGGCTTTTTTATGGTAATCTAATGAATGTATTGAGGTTATTATAGAATATACAAATAGGTTGCCGTTTTTTGTTTAAAAATTTTTAACAATTTTTTAAAATAAAGAAGGATAGAAAGACCCGGTGGTGGTTATGGTATTACTTc   >  1:2209198/1‑150 (MQ=255)
                                                                                                                                                    aCGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTca  <  2:2239210/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTTCGTGCCGCGTCTGATGCTGCCGATTTCTCTCTCCTTCGACCACCGCGTGATCGACGGTGCTGATGGTGCCCGTTTCATTACCATCATTAACAACACGCTGTCTGACATTCGCCGTCTGGTGATGTAAGTAAAAGAGCCGGCCCAACGGCCGGCTTTTTTCTGGTAATCTCATGAATGTATTGAGGTTATTAGCGAATAGACAAATCGGTTGCCGTTTGTTGTTTAAAAATTGTTAACAATTTTGTAAAATACCGACGGATAGAACGACCCGGTGGTGGTTAGGGTATTACTTCA  >  NC_000913/127457‑127754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: