Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,005 A→C 6.8% intergenic (‑124/+64) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0050AC 6.8% 850.9 / 5.5 296intergenic (‑124/+64)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (231/34);  new base C (2/27);  total (234/62)
Fisher's exact test for biased strand distribution p-value = 4.16e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153
                                                                                                                                                     |                                                                                                                                                     
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATATGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                       >  1:1711510/1‑150 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                      <  2:4955121/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                    <  1:3050153/150‑1 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGCAAACAAAGAGGaa                                                                                                                                                 >  2:3663239/1‑150 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                 >  1:6968665/1‑150 (MQ=255)
       cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaaa                                                                                                                                                >  1:2276643/1‑150 (MQ=255)
         tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                                                             <  1:1896218/150‑1 (MQ=255)
                tACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggg                                                                                                                                      >  2:4366474/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGCGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAACGGGGGGGGGGCt                                                                                                                                   >  2:5979138/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGACTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCt                                                                                                                                   >  1:5070574/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCt                                                                                                                                   >  2:5486519/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAACGGGGGGGGGGCTaa                                                                                                                                 >  2:1226588/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGTaa                                                                                                                                 >  1:7724524/1‑150 (MQ=255)
                     ggCATTTCACACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                 >  1:2415297/1‑150 (MQ=255)
                      gCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAATGAGGACAgggggggggggtcat                                                                                                                                >  2:1072626/1‑145 (MQ=255)
                      gCATTTCGCACCTCATAATCTTCTTGCATTTTACTATAGGTTGTTATTCTATATGTCAATGCATACCCCCCTTTAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCTGAAAACAATGAGGAAAGGGGGGGGGCTAATc                                                                                                                               <  2:3416529/150‑1 (MQ=255)
                          ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGAATAGGc                                                                                                                            >  1:3828493/1‑150 (MQ=255)
                              cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggg                                                                                                                        >  1:5109172/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGACTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTACTCGGCAGGGaa                                                                                                                      >  2:3951007/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGa                                                                                                                       >  2:186620/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                     >  1:5326526/1‑150 (MQ=255)
                                  tcatcaTCTGCATGCAATTTACGATTGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATACCACGGGAGAATAAAAATTATCCGGTGCATGGGGTCGGAAACCAAAGAGGAACGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                    <  2:4068022/150‑1 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAAAAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGCAAGGGGGGGGGGGTAATCGGCAGGGGAgg                                                                                                                    >  2:6229203/1‑150 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTAGAGTATATAACCCTGGAGAATAAAATTTATCCGGTGACTGTGGTCGGAGATCACCGAGGAAGGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                    >  2:7320863/1‑150 (MQ=255)
                                   cttgatcTGCATGCATTATACTATAGGGGGGTATTCTTTATGTCAATTCATACCCCCCTATATTTTTTAACTCTGTATAATAAATTTTATCCGTTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                                                   <  2:264768/146‑1 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGCAGgccg                                                                                                                 >  1:6633183/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                           >  2:7553527/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                           >  1:7325776/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                           >  2:8158307/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                          >  1:4386583/1‑150 (MQ=255)
                                            catgcaATCTACTATAGGGGTGTATTCTATATTACAATGCATACCCCCATATATAATTTAACACTGGAGAATAAAATTTATCCGGTGAAGGTGGCCGGAAAACAAAGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                          <  2:3823512/150‑1 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCCGGGAAGGCCGCCCCGGa                                                                                                          >  2:2368301/1‑150 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                          >  2:5365295/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                        >  2:4491373/1‑150 (MQ=255)
                                              tgcaATATACTACAGGGTGTTATTCTATATGTCAATGAATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTACCCGGTGATTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                        <  2:6360598/150‑1 (MQ=255)
                                                 aaTAGACTCTTGGGGGGTATTCTATATGTCACGGCATACCCCCCTATAGTATATACCACTGGAGAATAAAATTCACCCGGGGAATGTGGTCGGACCCCCCCGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                     <  2:5649275/150‑1 (MQ=255)
                                                  atatACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                     >  1:8106556/1‑150 (MQ=255)
                                                      aCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcgg                                                                                                >  1:870911/1‑150 (MQ=255)
                                                          taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                                                            >  1:2814232/1‑150 (MQ=255)
                                                            gggggtTATTCTATCTGTCAAGGCATACCCCCCTATAGTATATAACACTGGAGACTAAACATTATCCGGTGAATGTGGTCGGCAAACCCAGCGGACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                                                          <  2:7434304/150‑1 (MQ=255)
                                                                    ttCTCTATGTCCATGCATACCCCCCTAGAGTATCTAACCCTGGCGCCTACACTTGATCCGGTGACGGGGGTCGGAACCCCCCGCGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                                  <  2:6789419/150‑1 (MQ=255)
                                                                    ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                                  >  2:3691209/1‑150 (MQ=255)
                                                                    ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                                  >  2:7777069/1‑150 (MQ=255)
                                                                     tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                                                 >  1:6470160/1‑150 (MQ=255)
                                                                        atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                                                              >  1:108244/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCGGAATTTc                                                                             >  1:6759295/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                             >  1:5740628/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                             >  1:7526782/1‑150 (MQ=255)
                                                                          atGTCAATGCACACCCCCCTAGAGTATATAACACTGGAGAATAACATTGACCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           <  1:1192754/150‑1 (MQ=255)
                                                                           tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           >  1:7195714/1‑150 (MQ=255)
                                                                           tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCAGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           >  1:1668592/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                         >  2:7074142/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTc                                                                       >  2:352641/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  2:309482/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                      >  1:4898336/1‑150 (MQ=255)
                                                                                agcactACCCCCCCATATTATATAACCCTGGAGAACACACTTCATCCGGCGAAGGGGGCCGCACCACCCCCCCGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                      <  2:2842451/145‑1 (MQ=255)
                                                                                aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                      >  1:3416926/1‑150 (MQ=255)
                                                                                 tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                                                     >  1:2552543/1‑150 (MQ=255)
                                                                                 tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGACTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATATCCAGGTCAg                                                                     >  1:4757006/1‑150 (MQ=255)
                                                                                  gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                    >  1:5993726/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGAAATCGGCAGGGAAGCCCCCCCCGGATACCGGGCGGCCTAAGGAAACCGAATTGCCCGGCCCGACgggag                                                              >  1:6800057/1‑148 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  2:475150/1‑150 (MQ=255)
                                                                                          cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                            >  1:3326303/1‑150 (MQ=255)
                                                                                               aagaatataACACTGGAGAATAAAATTAATACGGTGAATGTGGTCGGAAAACAAATAGGAAAGGGGGTGGGGATAATCGGAAGGGAAGGCCGCACCGGAAAGCGGGCGGCAGAAGGAATCAGAATTACCAGGTCAGACGGGCTGCAAGtt                                                       >  2:6052173/5‑150 (MQ=255)
                                                                                                    tataACACTGGAGAATAAAATTTAGCCGGTGAACGGGGCCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                                  <  2:6559629/150‑1 (MQ=255)
                                                                                                     ataACACTGGATCATAATATTGATCCGGTGAATGTGGTCGGAAACCACCGCGGACCGGGGGGGGGATCATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc                                                <  1:1220443/150‑1 (MQ=255)
                                                                                                     ataACACTGGAGCATAACATTTCTCCGGGGCCGGCGCTCGCAAAACCCCGAGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAgcc                                                 <  2:2276643/150‑3 (MQ=255)
                                                                                                      taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 >  1:2686862/1‑150 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                               >  1:4496686/1‑150 (MQ=255)
                                                                                                          acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTa                                            >  2:6851701/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                          >  1:4252372/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                          >  1:5274796/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                          >  1:6111725/1‑150 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                        >  1:7098238/1‑150 (MQ=255)
                                                                                                                  aTAAAATTCACCCGGTGAAGGTGGTCGGAAAACCACCCGGACAGGGGGGGGGGCGAAGCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                                    <  2:3203389/150‑1 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCCAGTTGCAGACCGTTAAAATCATCg                                   >  1:5545908/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  1:1854836/1‑150 (MQ=255)
                                                                                                                    aaaaTTAATCCGGTGAATGTGGTCGGAAAACAAACAGGAAAGGGGGGGGCGCAAATCCGCCCGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTCCAGACCGTTAAAATCATCgg                                  >  2:2946295/1‑150 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCggg                                 >  1:756046/1‑149 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 >  1:6089767/1‑150 (MQ=255)
                                                                                                                      acctcacccGGTGAAGGTGGGCGGCAACCCACGCGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGtt                                <  2:7993980/143‑1 (MQ=255)
                                                                                                                            tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg                          >  1:5306266/1‑150 (MQ=255)
                                                                                                                                 tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                     >  1:6049011/1‑150 (MQ=255)
                                                                                                                                  gAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc                    >  1:2204504/1‑150 (MQ=255)
                                                                                                                                  gAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc                    >  1:3227039/1‑150 (MQ=255)
                                                                                                                                       tgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                     <  2:5286025/144‑1 (MQ=255)
                                                                                                                                       tgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac               >  2:5202012/1‑150 (MQ=255)
                                                                                                                                       tgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGGTGGGGTGTCGTACCacac               >  2:4873125/1‑150 (MQ=255)
                                                                                                                                        ggTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGGGTCGTACCacac               >  2:5809694/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  2:6405558/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  1:2937905/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  1:1293579/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc          >  2:2172813/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc          >  2:7247449/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCAGCGGTTGGGGTGTCGTACCACACTTTAc          >  2:7710422/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTCAAATCAGCGGTGGGGGTGTCGTACCACCCTTTAc          >  2:4236750/1‑150 (MQ=255)
                                                                                                                                               aaaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCCCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTg       >  1:147919/1‑150 (MQ=255)
                                                                                                                                                 aCAACGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGcc     <  2:7167872/150‑1 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  2:2978454/1‑150 (MQ=255)
                                                                                                                                                    aaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  1:6254089/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: