Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 624,610 A→T 7.5% intergenic (‑100/‑275) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913624,6100AT 7.5% 1179.3 / 5.9 416intergenic (‑100/‑275)fepB/entCferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base A (248/103);  new base T (3/43);  total (251/165)
Fisher's exact test for biased strand distribution p-value = 1.25e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

AAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGAT  >  NC_000913/624461‑624759
                                                                                                                                                     |                                                                                                                                                     
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  1:5409863/150‑1 (MQ=255)
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  1:5863816/150‑1 (MQ=255)
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  1:7475399/150‑1 (MQ=255)
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  1:3477896/150‑1 (MQ=255)
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  1:6252637/150‑1 (MQ=255)
 aaaGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                                                                                                                      <  2:242499/150‑1 (MQ=255)
   aGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                                    >  2:1353932/1‑150 (MQ=255)
     tCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATCAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTATGGGGCGAGGGTTAGGGTGAGGGGa                                                                                                                                                  >  2:6381520/1‑150 (MQ=255)
         gTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcag                                                                                                                                              >  1:2527705/1‑150 (MQ=255)
           tAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCGTCCTGTTATTAAGAAGGTGAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGTGAGGGGAAcagcc                                                                                                                                            >  2:1612812/1‑150 (MQ=255)
            aaTAGATGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTTGGGTGAGGGGAAcagcca                                                                                                                                           <  2:6863128/150‑1 (MQ=255)
            aaTAGAAGGGCGTTGCGGTAGAGCGGGGCTAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTATTGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGTGGGTTAGGGTGAGGGGAAcagcca                                                                                                                                           <  2:4744971/150‑1 (MQ=255)
            aaTAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagcca                                                                                                                                           >  1:206071/1‑150 (MQ=255)
             aTAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagccag                                                                                                                                          >  2:546084/1‑150 (MQ=255)
                  cgggCGTTGCGGTAGTGCGGGGCGAGTCTCAAAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAATGCGCACAATCCTTCCCCTCGCCCCTTTGGGTAGTGGGTTTGGGTGTGGGGAACAGCCAGCACTg                                                                                                                                     <  2:1099824/149‑1 (MQ=255)
                  aGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCCGCTTCCTGTTATTATTATGGTTATGGGCTTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGTGAGGGTTTGGGTGTGGGGAACAGCCAGCACTg                                                                                                                                     <  2:1316033/150‑1 (MQ=38)
                      cGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATACGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGc                                                                                                                                 <  2:5229430/150‑1 (MQ=255)
                        ttGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGa                                                                                                                               >  1:6840946/1‑150 (MQ=255)
                              tAGAGCGGGGCGAGTCTCACAATTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGTGGGTTAGGGTGTGGGGAACAGCCAGCACTGGTGCGAGCATTa                                                                                                                         >  1:314849/1‑150 (MQ=255)
                              tAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTa                                                                                                                         >  1:6977953/1‑150 (MQ=255)
                              tAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTa                                                                                                                         >  2:348019/1‑150 (MQ=255)
                              tAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTa                                                                                                                         >  2:7734430/1‑150 (MQ=255)
                              tAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTa                                                                                                                         >  1:5707821/1‑150 (MQ=255)
                                    ggggCGCGTCTCACAAATCCGCTTCCTTTTATTATTAGGGTTATGGGCGTAATGACAAATTCGACAATGCGCACAATCGGTCCCCTCGCCCCTTTGGGGTGAGGGTTTGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTc                                                                                                                   <  2:1722937/150‑1 (MQ=37)
                                    ggggCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTc                                                                                                                   >  2:6915805/1‑150 (MQ=255)
                                       gCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAcc                                                                                                                >  2:4914884/1‑150 (MQ=255)
                                       gCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAcc                                                                                                                >  1:5306527/1‑150 (MQ=255)
                                        cGAGTCTCACAAACCCGCTTCCTGTTATTAATCAGGTTACGGGCGTAATGACAATTTCTAAAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGTTGTGGGTTTGGGTGCGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAccc                                                                                                               <  2:4193950/150‑1 (MQ=37)
                                            tctcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGc                                                                                                           >  1:1234479/1‑150 (MQ=255)
                                              tcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGccc                                                                                                         >  1:4166370/1‑150 (MQ=255)
                                              tcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCATTTGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGACCATTAACCCACACCCCAGccc                                                                                                         >  1:5011529/1‑150 (MQ=255)
                                                acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGGCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTc                                                                                                       >  2:3954693/1‑150 (MQ=255)
                                                acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTc                                                                                                       >  1:6695939/1‑150 (MQ=255)
                                                acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTc                                                                                                       >  1:7002991/1‑150 (MQ=255)
                                                 caAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGTGGGTTAGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCa                                                                                                      <  2:1191430/150‑1 (MQ=255)
                                                  acacacgCTTCCTGTTATAAATACGGTACTGGGGTTAATGACAACTTCCAAAAAGCGCACACCCCGCCCCCTCCCCCCTTTGGGTTTTGGTTTCGGGTGGGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAc                                                                                                     <  2:2134724/144‑1 (MQ=255)
                                                    aTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGc                                                                                                                                 >  1:3882810/1‑120 (MQ=255)
                                                       aGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTgg                                                                                                >  1:1609874/1‑150 (MQ=255)
                                                          ttCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAg                                                                                             >  1:7265782/1‑150 (MQ=255)
                                                              tGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTCACCCTCACCCCAGCCCTCACCCTGGAAGGgag                                                                                         >  2:7090468/1‑150 (MQ=255)
                                                              tGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGgag                                                                                         >  2:7468139/1‑150 (MQ=255)
                                                              tGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGgag                                                                                         >  1:4749333/1‑150 (MQ=255)
                                                                ttattaATAAGGTTATGGGCGTATTGACAAATTCGACAAAGCGCACAATCCGTCCCCGCGCCCCTTTGGGGAGAGGGTTCGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGagag                                                                                       <  2:8062366/150‑1 (MQ=255)
                                                                ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGagag                                                                                       >  1:3338540/1‑150 (MQ=255)
                                                                   ttaATACGTTTCCGGGCTTAATCAAAAATCCGACAACGCGCACAATCCGCCCCCTCGCCCGTGTGGGTTGTGGTTTTGGGTGCGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAgggg                                                                                    <  1:1572373/150‑1 (MQ=255)
                                                                     aataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGc                                                                                  >  2:6754828/1‑150 (MQ=255)
                                                                      ataaGGTTAAGGGCGTAATGCCACTTTCGAAAAAGCGCCCAATCCGTCCCCTCGCCCCTTTGGGGAGCGGTTTCGGGGGCGGGTAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCTCCCTTCCAGGGTGAgggctgg                                                                                 <  1:2780833/150‑5 (MQ=21)
                                                                      ataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCa                                                                                 >  1:31235/1‑150 (MQ=255)
                                                                        aaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGa                                                                               >  2:5979561/1‑150 (MQ=255)
                                                                        aaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGa                                                                               >  2:827141/1‑150 (MQ=255)
                                                                            ttACGGGCGTAATGACAACTTCGACAACGCGCACAACCCGTCCCCGCGCCCCTTGGGGGTGTGGTTTGGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACgg                                                                           <  1:3739778/150‑1 (MQ=255)
                                                                             tacgggCGTAATCAAAAATCCCACAACGCGCAAAATCCGTCCCCCCGCCCTTTGGGGTTGTGGGTTCGGGTTTGGGCAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGgc                                                                          <  1:447425/147‑1 (MQ=255)
                                                                                gggCTTAATTAAAAATTCTACAACGCGCAAAATCCGCCCCCTCGCCCTTTTGGGGTGTGGTTTCGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCa                                                                       <  1:1727702/150‑1 (MQ=255)
                                                                                gggCTTAATGACAAATTCCACAACGCCCACAATCCTGCCCCGCGCCCCTTGGGGGTGTGGTTTTGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCa                                                                       <  1:5623350/150‑1 (MQ=255)
                                                                                gggCGTAATGACAACTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCa                                                                       <  2:2005525/150‑1 (MQ=255)
                                                                                gggCGTAAGGACAAATTCGACACAGCGCCCAAGCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCTCCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCa                                                                       <  2:4962929/150‑1 (MQ=25)
                                                                                  gCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAgg                                                                     >  1:6446951/1‑150 (MQ=255)
                                                                                  gCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAgg                                                                     >  1:3078371/1‑150 (MQ=255)
                                                                                   gttAATGACAACTTCCACAAAGCGCACAACCCGACCCCTCGCCCCTTTGGGGAGTGGTTTAGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGa                                                                    <  2:5707539/148‑1 (MQ=25)
                                                                                    ttAATTACAATTTCGACAACGCGCACAATCCGTCCCCTCGCCCCTTTGGGTTGAGGGTTTGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGAc                                                                   <  2:712697/149‑1 (MQ=25)
                                                                                        tGACAAATTCGACAAAGCGCACAATCCGTCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATcac                                                              >  2:3830283/1‑150 (MQ=255)
                                                                                            aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACAtt                                                           >  2:1888417/1‑150 (MQ=255)
                                                                                               ttccaaaaaGCGCAAAACCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGCAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                                        <  1:4592296/144‑1 (MQ=25)
                                                                                               ttCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTgcg                                                        >  2:4301031/1‑150 (MQ=255)
                                                                                                  gACAAAGCGCACAATCCGTCCCCTCGCCCCGTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGACCAGTAACCCTCACCCCAGCCCTCACCCGGGAAGGGAGAGGGGGCCGAACGGCGCAGGACCTCACAGTgcgcgt                                                     >  1:2252685/1‑148 (MQ=25)
                                                                                                    cAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGTGGGTTTGGGTGCGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTAt                                                   <  1:3269621/150‑1 (MQ=255)
                                                                                                      aaGCGCACAATCCGTCCCCTCGCCCCTTTGGGTAGTGGTTTTGGGTGAGGGTAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                                 <  2:4042311/150‑1 (MQ=255)
                                                                                                      aaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTCGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                                 <  2:6152502/150‑1 (MQ=255)
                                                                                                      aaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGc                                                 >  2:6067557/1‑150 (MQ=255)
                                                                                                       aGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCg                                                >  2:5105227/1‑150 (MQ=255)
                                                                                                        gcgcACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGa                                               <  2:3303816/150‑1 (MQ=255)
                                                                                                            acaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCCGAACGGCGCAGGACATCACATTGCGCTTATGCGAATcc                                           >  1:1505418/1‑150 (MQ=255)
                                                                                                             caATCCGTCCCCTCGCCCTTTTGGGGAGTGGTTTCGGTTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCa                                          <  2:2981555/150‑1 (MQ=255)
                                                                                                              aaaccGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCAt                                         <  2:1065426/147‑1 (MQ=255)
                                                                                                              aaTCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCAt                                         >  1:1063744/1‑150 (MQ=255)
                                                                                                                  cGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaat                                     >  1:5467107/1‑150 (MQ=255)
                                                                                                                   gTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaata                                    >  2:2485056/1‑150 (MQ=255)
                                                                                                                   gTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaata                                    >  1:7924669/1‑150 (MQ=255)
                                                                                                                      cccTCGCCCCTTTGGGGAGAGGGTTAGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                                 <  1:1964378/150‑1 (MQ=255)
                                                                                                                      cccTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                                 >  2:2643031/1‑150 (MQ=255)
                                                                                                                      cccTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATg                                 >  1:1416899/1‑150 (MQ=255)
                                                                                                                       ccTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACCTCACAGTGCGCTTATGCGAATCCATCAATAATGc                                >  2:3782443/1‑150 (MQ=255)
                                                                                                                        cTCGCCCCTTTGGGGTGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCt                               <  1:4852823/150‑1 (MQ=255)
                                                                                                                            ccccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctc                           >  1:8035431/1‑150 (MQ=255)
                                                                                                                                 ttGGTGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAtttt                      <  2:4576842/150‑1 (MQ=255)
                                                                                                                                  tGGGGAGTGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTc                     <  1:7785711/150‑1 (MQ=255)
                                                                                                                                    gggAGAGGGTTTGGGTGTGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                   <  1:973563/150‑1 (MQ=255)
                                                                                                                                      gagaGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTg                 >  2:3385671/1‑150 (MQ=255)
                                                                                                                                      gagaGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTg                 >  1:2707820/1‑150 (MQ=255)
                                                                                                                                         tgggTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTaa              <  1:6186017/149‑1 (MQ=255)
                                                                                                                                           ggTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAAcc            >  2:7344354/1‑150 (MQ=255)
                                                                                                                                            gTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACca           >  1:7613367/1‑150 (MQ=255)
                                                                                                                                                gggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAc       >  2:6535993/1‑150 (MQ=255)
                                                                                                                                                 ggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc      <  2:2773078/150‑1 (MQ=255)
                                                                                                                                                 ggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc      >  1:6376439/1‑150 (MQ=255)
                                                                                                                                                   tGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTACCCACAACCAg    >  2:6301736/1‑150 (MQ=255)
                                                                                                                                                   tGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAg    >  2:4839762/1‑150 (MQ=255)
                                                                                                                                                    gAGGGGCACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGa   <  2:2553953/150‑1 (MQ=255)
                                                                                                                                                    gAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGa   >  1:8221293/1‑150 (MQ=255)
                                                                                                                                                     aGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGAt  <  1:1326344/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAAGTCCTGTTAATAGAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGAT  >  NC_000913/624461‑624759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: