Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,015 T→A 7.2% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0150TA 7.2% 1450.2 / 13.9 494intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base T (161/278);  new base A (48/4);  total (210/284)
Fisher's exact test for biased strand distribution p-value = 2.22e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 515 total aligned reads displayed.

ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164
                                                                                                                                                     |                                                                                                                                                     
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                       <  2:5115152/150‑1 (MQ=255)
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAt                                                                                                                                                       >  1:5195887/1‑150 (MQ=255)
aTGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTACGCTTCGCAAt                                                                                                                                                       >  1:126001/1‑150 (MQ=255)
 tGATCTCTGCAATATATTGTGTTTGCGTGCTTTTGTAGGCCGGATATGGCGTTCCCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAtt                                                                                                                                                      <  1:6339784/150‑1 (MQ=255)
   aTCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTa                                                                                                                                                    >  1:5526413/1‑150 (MQ=255)
   aTCTCTGCAATATAGTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTa                                                                                                                                                    <  2:7057646/150‑1 (MQ=255)
    tctcTGCAATTTTTTGAGTTTGCGTGCTTTTGTAGGCCGGATATGGCGTTCACGCCGAATCGGTCAAGAAACTGCAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTAt                                                                                                                                                   <  1:5078329/150‑1 (MQ=38)
     ctctGAAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCTTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                  <  1:803546/150‑1 (MQ=255)
         gCAATAGATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAAGAAACAGCAAACAAGCCACAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCata                                                                                                                                              <  1:3401247/150‑1 (MQ=255)
          cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                             >  1:8125520/1‑150 (MQ=255)
           aaTATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaa                                                                                                                                            <  1:1028007/150‑1 (MQ=255)
                atTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaataaa                                                                                                                                       <  1:4655072/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAATTATCCATAaaataaat                                                                                                                                      >  2:3135773/1‑150 (MQ=255)
                    aGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTa                                                                                                                                   <  2:7262768/150‑1 (MQ=255)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTCTTAGCGAATTAATTACGCTTAGCAATTTATACATAAAATAAAATTaa                                                                                                                                  >  1:3015975/1‑150 (MQ=38)
                     gTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTAGCGAATTAATTCAGCTTAGCAATTTATACATAAAATAAATTTaa                                                                                                                                  >  1:4333404/1‑150 (MQ=38)
                      tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAAAAATCCAAAACGCCGCGTTCAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaa                                                                                                                                 <  1:2382225/150‑1 (MQ=255)
                          cttGCTTTTGTCGGCCGGATAAGGCTTTCACGCCTCATCCGGCAAGAAACAGAAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  1:1540011/148‑1 (MQ=255)
                          cgtccatttGAAGGCCCGAAAAGGCGTACACGCCGCAACCGCCAACAAACACCAAACAAACCAAAACGCCGCGAACAGCGGCGAATTTTCTGCTTTTCTTCGCGAATTAATACCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  2:2033383/144‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  1:6924057/150‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             >  2:5187692/1‑150 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATaa                                                                                                                             <  1:7850454/150‑1 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTTTGGTTTTGTTATGGTATTTATTAAGGTTGGGAATTTATGCATAAAATAAATTTTATATTaaaaaa                                                                                                                        >  1:1709127/1‑150 (MQ=21)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAAATTATCCATAAAATAAATTTAAAATAaaaaaa                                                                                                                        >  1:7226279/1‑150 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTGTTCGCGAAATAATTCCGCGTCGCCATTTATACATAAAAAAAATATAAAATAaaaaaa                                                                                                                        >  1:5170423/1‑150 (MQ=38)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                   <  1:2186240/150‑1 (MQ=255)
                                    tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTCCGCATCGCAATTTATCCATAAAATAAATATAAAATAACAAAACATaa                                                                                                                   >  2:3392180/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGGTTTTGTTCGCGAATTAATTCCGCGTCGCCATTTATACATAAAATAAATTTAAAATAACACAACATAAAta                                                                                                                >  1:5630630/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                >  2:4037768/1‑150 (MQ=255)
                                       gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTCGCGAATTAATTACGCGTAGCAAATTATACATAAAATAAAATTAAAATAAAAAAACAAAAAta                                                                                                                >  1:3153016/1‑150 (MQ=255)
                                        ccGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaa                                                                                                               <  2:2704353/150‑1 (MQ=255)
                                        ccGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCATCGCCATTTATACAAAAAATAAATTTAAAATAACAAAACATAATtaa                                                                                                               >  1:6023470/1‑150 (MQ=255)
                                        ccGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGCTTTTATTCGCGAATTAATTCCGCTTCGCAATTTATACATAAAATAAATTTTAAATTAAAAAACATAATtaa                                                                                                               >  1:7509371/1‑150 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                              <  1:2919624/150‑1 (MQ=255)
                                             tAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaataaa                                                                                                          <  1:5319687/150‑1 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  1:5821937/150‑1 (MQ=255)
                                              aaGGCGTACACGCCGCATCCGGCAAGAAACAGCAAACAATACAAAACGCCGCTTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  1:4882343/150‑1 (MQ=255)
                                                ggCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATg                                                                                                       <  2:1057821/150‑1 (MQ=255)
                                                   gTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTaa                                                                                                    <  2:6602434/150‑1 (MQ=255)
                                                   gTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGGGTTTGTTAGCGAAATAATTACGCTTCGCAATTTATACATAAAATAAATTTAAAATAAAAAAAAAAAAATAAAAAAAATTTaa                                                                                                    >  1:3348657/1‑150 (MQ=255)
                                                      cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCg                                                                                                 >  2:1933365/1‑150 (MQ=255)
                                                         gccgcATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCtt                                                                                              <  1:1084181/150‑1 (MQ=255)
                                                          ccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCttt                                                                                             >  2:5876968/1‑150 (MQ=255)
                                                                ccGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCtt                                                                                       <  2:6109880/150‑1 (MQ=255)
                                                                ccGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAATAAATAAAATTTAACCGCGTTCATAtt                                                                                       >  2:1112806/1‑150 (MQ=255)
                                                                 cGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTg                                                                                      <  1:6482383/150‑1 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                    <  2:3295414/150‑1 (MQ=255)
                                                                     aaGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAAAAAAAATAAATAAAAAGTAAACGCTTTCATATTTCTgg                                                                                  >  2:1600240/1‑150 (MQ=255)
                                                                       gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGaa                                                                                <  2:2422893/150‑1 (MQ=255)
                                                                       gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGaa                                                                                <  2:5499432/150‑1 (MQ=255)
                                                                           cAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTc                                                                            <  1:6598839/150‑1 (MQ=255)
                                                                           cAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTc                                                                            <  1:2750817/150‑1 (MQ=255)
                                                                              cAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                         <  1:974333/150‑1 (MQ=255)
                                                                                    aTCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAACTTATCCATAAAATAAATTTAAAATAACAAAACATAATGAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTa                                                                   <  2:4589917/150‑1 (MQ=255)
                                                                                     tCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAt                                                                  >  2:4756510/1‑150 (MQ=255)
                                                                                          aaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAAAATAATTAAAAAAAATTTAAACGCTTTAATATTGATGGAATTTCACGCTTTTAttattc                                                             >  2:3740155/1‑150 (MQ=255)
                                                                                            cgccgcGTTCAGCGGCGTTTTTTGTGCTTTTCTTAGCGAATTAATTCCGCGTCGCAATTTATCCATAAAAAAAAATTAAAATAACAAAAAAAAAATAAATAAAATGTAAACGCTTTCATCTTTCTGGAATTTAACGCTTTTATTATTATg                                                           >  2:142741/1‑150 (MQ=255)
                                                                                             gccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGc                                                          >  2:6868657/1‑150 (MQ=255)
                                                                                              ccgcGTTCAGCGGCGTTTTTTATGCTTTTATTCGCGAATTAAATCCGCTTAGCAAATTATACATAAAAAAAATTTAAAATAACAAAACATAAATAAATAAAATGTAACCGCATTCATCTTTCTGGTAGTTAACGAATTTATTGTTCTGCa                                                         >  2:754584/1‑150 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGc                                                      <  2:8236800/150‑1 (MQ=255)
                                                                                                  gTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGcc                                                     <  1:182873/150‑1 (MQ=255)
                                                                                                   ttCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCt                                                    <  1:6682515/150‑1 (MQ=255)
                                                                                                    tCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCtt                                                   <  1:4528937/150‑1 (MQ=255)
                                                                                                     catcggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGATTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCttt                                                  <  2:1494235/147‑1 (MQ=255)
                                                                                                     cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTAGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCttt                                                  <  2:3061187/150‑1 (MQ=255)
                                                                                                      aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTACTTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTc                                                 <  2:5652969/150‑1 (MQ=255)
                                                                                                      aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTc                                                 <  1:1431853/150‑1 (MQ=255)
                                                                                                            gTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGc                                           >  2:7843290/1‑150 (MQ=255)
                                                                                                             ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                          >  2:6208343/1‑150 (MQ=255)
                                                                                                               ttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCaaa                                        <  1:4783752/150‑1 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  2:8187362/150‑1 (MQ=255)
                                                                                                                    tGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTaa                                   <  2:4528705/150‑1 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAACCATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGTATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 <  2:4333283/150‑1 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 >  2:6544526/1‑150 (MQ=255)
                                                                                                                      cTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGc                                 <  2:5563556/150‑1 (MQ=255)
                                                                                                                       ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                >  1:1390024/1‑150 (MQ=255)
                                                                                                                       ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                >  2:529555/1‑150 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCTCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  2:3617927/150‑1 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCGCTTCTCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  1:7680484/150‑1 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                           <  1:7407251/150‑1 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                           >  1:5745947/1‑150 (MQ=255)
                                                                                                                             tcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTaa                          <  2:6743718/150‑1 (MQ=255)
                                                                                                                              cgcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAAc                         >  1:1123434/1‑150 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      >  2:3824038/1‑150 (MQ=255)
                                                                                                                                  aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                     <  1:2496553/150‑1 (MQ=255)
                                                                                                                                      aattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatca                 <  1:7052323/150‑1 (MQ=255)
                                                                                                                                       attCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTATATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcat                >  2:4887346/1‑150 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  2:1247228/150‑1 (MQ=255)
                                                                                                                                        gtCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  1:201151/149‑1 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              <  1:4296346/150‑1 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              >  2:2273431/1‑150 (MQ=255)
                                                                                                                                           cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa            >  1:3756349/1‑150 (MQ=255)
                                                                                                                                            gCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAACGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaaa           >  2:4293895/1‑150 (MQ=255)
                                                                                                                                               tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        <  2:568870/150‑1 (MQ=255)
                                                                                                                                               tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        >  1:1968424/1‑150 (MQ=255)
                                                                                                                                               tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        >  1:3924186/1‑150 (MQ=255)
                                                                                                                                               tCGCAACTTATCCATAAAATAAATTTAAAATAACAACACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat        <  1:5574402/150‑1 (MQ=255)
                                                                                                                                                 gCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgt      <  1:3538472/150‑1 (MQ=255)
                                                                                                                                                 gCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgt      >  1:3579297/1‑150 (MQ=255)
                                                                                                                                                    aTTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCg   <  2:7959761/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: