Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,886 T→G 90.2% L327R (CTG→CGG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,8860TG90.2% 1676.2 / 120.9 481L327R (CTG→CGG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (19/29);  new base G (210/223);  total (229/252)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

CCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAA  >  NC_000913/756738‑757034
                                                                                                                                                    |                                                                                                                                                    
 cGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGc                                                                                                                                                    >  1:5293639/1‑150 (MQ=255)
  gAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGcc                                                                                                                                                   >  2:3775339/1‑150 (MQ=255)
    aCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCgg                                                                                                                                                 <  2:4648643/150‑1 (MQ=255)
    aCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCgg                                                                                                                                                 <  1:3006354/150‑1 (MQ=255)
      gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGt                                                                                                                                               >  1:162872/1‑150 (MQ=255)
         aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATc                                                                                                                                            <  2:5823514/150‑1 (MQ=255)
         aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATc                                                                                                                                            >  1:5358783/1‑150 (MQ=255)
           aGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCt                                                                                                                                          <  1:5530862/150‑1 (MQ=255)
              ccTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGa                                                                                                                                       <  1:1002208/150‑1 (MQ=255)
                 ggCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCt                                                                                                                                    >  2:7384528/1‑150 (MQ=255)
                     ggCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTcc                                                                                                                                <  2:1176528/150‑1 (MQ=255)
                         gTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTa                                                                                                                            <  2:6939263/150‑1 (MQ=255)
                         gTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTa                                                                                                                            <  2:4216821/150‑1 (MQ=255)
                          tGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTAc                                                                                                                           <  2:3304362/150‑1 (MQ=255)
                           gACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTAcc                                                                                                                          >  1:370122/1‑150 (MQ=255)
                               tGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg                                                                                                                      <  2:5719517/150‑1 (MQ=255)
                                ggTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                                                                     <  2:6128190/150‑1 (MQ=255)
                                ggTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                                                                                                     <  1:392068/150‑1 (MQ=255)
                                  ttGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTc                                                                                                                   >  1:6306852/1‑150 (MQ=255)
                                  ttGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTc                                                                                                                   >  2:3719656/1‑150 (MQ=255)
                                    gcgcGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCAc                                                                                                                 <  2:1020525/150‑1 (MQ=255)
                                     cgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACg                                                                                                                >  2:6567258/1‑150 (MQ=255)
                                           ccATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATc                                                                                                          <  2:5298631/150‑1 (MQ=255)
                                            catcatGATCGAAATCCGTGAATGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATcc                                                                                                         <  2:4868249/150‑1 (MQ=255)
                                               catGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                                                                                      <  1:3820171/150‑1 (MQ=255)
                                               catGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                                                                                      >  1:586945/1‑150 (MQ=255)
                                                atGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTg                                                                                                     >  1:3770466/1‑150 (MQ=255)
                                                  gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                                                                                   <  1:7978729/150‑1 (MQ=255)
                                                   aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa                                                                                                  >  1:5132121/1‑150 (MQ=255)
                                                   aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaaa                                                                                                  >  1:6914465/1‑150 (MQ=255)
                                                        aaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                             <  1:6643081/150‑1 (MQ=255)
                                                        aaTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGcc                                                                                             >  2:2328795/1‑150 (MQ=255)
                                                         aTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                            >  2:4078558/1‑150 (MQ=255)
                                                         aTCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg                                                                                            >  1:6819852/1‑150 (MQ=255)
                                                            cGTGAAGGTCGCTGCTGTGAAGGTCCGTGGGGGAAACACGAGAAACTGAAAAACGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCAGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGAtt                                                                                         >  2:8084238/1‑150 (MQ=255)
                                                              tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGTGCCGATTcc                                                                                       >  2:5069513/1‑150 (MQ=255)
                                                              tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTcc                                                                                       <  2:1245719/150‑1 (MQ=255)
                                                              tGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTcc                                                                                       >  2:1879262/1‑150 (MQ=255)
                                                               gAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                                                                      <  2:2650189/150‑1 (MQ=255)
                                                               gAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCg                                                                                      <  1:1350643/150‑1 (MQ=255)
                                                                   gTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                                                                                  >  1:5981391/1‑150 (MQ=255)
                                                                   gTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                                                                                  >  2:7098791/1‑150 (MQ=255)
                                                                     cgcgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATc                                                                                <  1:3785894/150‑1 (MQ=255)
                                                                       cgGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATccc                                                                              >  1:3290167/1‑150 (MQ=255)
                                                                         gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCTATTCCGGTTATCCCaa                                                                            <  1:5044066/150‑1 (MQ=255)
                                                                         gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCaa                                                                            <  1:2309106/150‑1 (MQ=255)
                                                                          cTGTGATGGTCCGTGGGGGCCACACGCGACACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAAGCCGGTCGGGCGGGTAGCCTGGAGCTTTCCCGTACCTTCGCTCTCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAc                                                                           <  1:4891863/150‑1 (MQ=255)
                                                                              gATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGt                                                                       >  1:2079673/1‑150 (MQ=255)
                                                                               aTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTc                                                                      >  1:2852795/1‑150 (MQ=255)
                                                                                tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCa                                                                     <  2:5496731/150‑1 (MQ=255)
                                                                                tGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCa                                                                     <  2:663922/150‑1 (MQ=255)
                                                                                 ggTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAc                                                                    >  1:4754800/1‑150 (MQ=255)
                                                                                  gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                                   <  1:4563158/150‑1 (MQ=255)
                                                                                  gTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACt                                                                   >  2:7622439/1‑150 (MQ=255)
                                                                                   tCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTa                                                                  <  1:7809071/150‑1 (MQ=255)
                                                                                    ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTAc                                                                 <  1:572274/150‑1 (MQ=255)
                                                                                         ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgat                                                            >  1:4019230/1‑150 (MQ=255)
                                                                                         ggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgat                                                            <  1:2907156/150‑1 (MQ=255)
                                                                                              cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                                       <  1:8079962/150‑1 (MQ=255)
                                                                                                    cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                                 >  1:7367769/1‑150 (MQ=255)
                                                                                                     gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                                >  2:902200/1‑150 (MQ=255)
                                                                                                      aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                               <  2:5566231/150‑1 (MQ=255)
                                                                                                      aaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCg                                               <  2:2078899/150‑1 (MQ=255)
                                                                                                       aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                              <  1:6185769/150‑1 (MQ=255)
                                                                                                       aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                              <  2:7553482/150‑1 (MQ=255)
                                                                                                       aactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGa                                              <  2:1775199/150‑1 (MQ=255)
                                                                                                        actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                             >  2:1663581/1‑150 (MQ=255)
                                                                                                        actgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGAc                                             >  1:4279100/1‑150 (MQ=255)
                                                                                                          tgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCa                                           >  2:3041525/1‑150 (MQ=255)
                                                                                                           gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaa                                          >  1:116417/1‑150 (MQ=255)
                                                                                                              actCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGt                                       >  2:7369924/1‑150 (MQ=255)
                                                                                                                 cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                    <  2:7190225/150‑1 (MQ=255)
                                                                                                                 cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                    <  1:90857/150‑1 (MQ=255)
                                                                                                                  gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                                   <  2:5373858/150‑1 (MQ=255)
                                                                                                                  gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                                   <  1:1820127/150‑1 (MQ=255)
                                                                                                                   aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCg                                  <  2:6736458/150‑1 (MQ=255)
                                                                                                                   aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCg                                  >  2:2466623/1‑150 (MQ=255)
                                                                                                                     cACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGt                                <  1:7149307/150‑1 (MQ=255)
                                                                                                                        cTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAg                             >  2:2195821/1‑150 (MQ=255)
                                                                                                                          gggTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGc                           >  1:2556577/1‑150 (MQ=255)
                                                                                                                            gTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCac                         >  2:6908880/1‑150 (MQ=255)
                                                                                                                               aagaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga                      <  2:5616826/150‑1 (MQ=255)
                                                                                                                               aagaagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactga                      <  1:5594402/150‑1 (MQ=255)
                                                                                                                                agaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac                     <  2:1530359/150‑1 (MQ=255)
                                                                                                                                agaagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgac                     <  2:5142655/150‑1 (MQ=255)
                                                                                                                                   agTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                  <  2:2838897/150‑1 (MQ=255)
                                                                                                                                   agTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                  <  2:391684/150‑1 (MQ=255)
                                                                                                                                     ttCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa                <  1:1732593/150‑1 (MQ=255)
                                                                                                                                     ttCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGa                >  1:4141356/1‑150 (MQ=255)
                                                                                                                                      tctcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGaa               <  2:628045/150‑1 (MQ=255)
                                                                                                                                        tcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATg             >  2:5955010/1‑150 (MQ=255)
                                                                                                                                        tcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATg             >  2:3389377/1‑150 (MQ=255)
                                                                                                                                        tcGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATg             >  2:3518927/1‑150 (MQ=255)
                                                                                                                                         cGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga            <  1:7273806/150‑1 (MQ=255)
                                                                                                                                           aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga          <  2:5276860/150‑1 (MQ=255)
                                                                                                                                           aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga          <  2:7914388/150‑1 (MQ=255)
                                                                                                                                             tCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa        >  2:4440976/1‑150 (MQ=255)
                                                                                                                                               ccGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAgg      >  1:3161084/1‑150 (MQ=255)
                                                                                                                                               ccGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAgg      >  1:4421228/1‑150 (MQ=255)
                                                                                                                                                cGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGc     >  1:6431820/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAA  >  NC_000913/756738‑757034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: