Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 777,208 A→C 6.5% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913777,2080AC 6.5% 1090.8 / 5.2 402K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (268/95);  new base C (3/34);  total (272/130)
Fisher's exact test for biased strand distribution p-value = 2.24e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  NC_000913/777060‑777356
                                                                                                                                                     |                                                                                                                                                     
 agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCCCAA‑Gc                                                                                                                                                    >  2:6026931/1‑150 (MQ=255)
 agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑Gc                                                                                                                                                    >  1:3683027/1‑150 (MQ=255)
  gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCg                                                                                                                                                   >  2:826583/1‑150 (MQ=255)
  gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCg                                                                                                                                                   >  2:356905/1‑150 (MQ=255)
  gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCg                                                                                                                                                   >  2:7301904/1‑150 (MQ=255)
     aaaaCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGcc                                                                                                                                                >  2:1091792/1‑150 (MQ=255)
        gCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGACAAAGCCGCTGCAGCACCA‑GCGGCCGCa                                                                                                                                             >  1:3376477/1‑150 (MQ=255)
          tGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCaga                                                                                                                                           >  2:1301687/1‑150 (MQ=255)
          tGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCaga                                                                                                                                           >  2:4811492/1‑150 (MQ=255)
            cAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAgg                                                                                                                                         >  1:8046867/1‑150 (MQ=255)
             aGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCCGCAGCTGATAAGAAAGCAGCGGCAGACAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCACAAGCTGCCGCGGACAAAGCCGCGGCAGCAACA‑GCGGCCGCAGCGGc                                                                                                                                        >  2:5925094/1‑150 (MQ=255)
                    aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAgatgat                                                                                                                                 >  2:743753/1‑150 (MQ=255)
                    aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAgatgat                                                                                                                                 >  2:1862114/1‑150 (MQ=255)
                    aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAgatgat                                                                                                                                 >  2:3130171/1‑150 (MQ=255)
                    aaaaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCAGCAGCAAAA‑GCGGCCGCAGAGGCAgatgat                                                                                                                                 >  2:1578846/1‑150 (MQ=255)
                     aaaaaGCAGCAGACAAAGCGGCTGCTTAAACGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAACCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGata                                                                                                                                <  2:7976645/150‑1 (MQ=255)
                      aaaaGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGatat                                                                                                                               >  1:6461591/1‑150 (MQ=255)
                          ccagcagAAAACGCGGCTGCTGAAACGGCAGCAGCTTATAAGAAAGCAGCGGCTGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTc                                                                                                                           <  2:7093367/149‑1 (MQ=255)
                             gcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGt                                                                                                                        >  1:2332492/1‑150 (MQ=255)
                             gcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGt                                                                                                                        >  1:1289985/1‑150 (MQ=255)
                             gcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGt                                                                                                                        >  1:1903720/1‑150 (MQ=255)
                                       cGGCTGCTTAAACGGCAGCAGCTGATAAGAAAGCAGCGGAAGAAAACGCCGCCGCAGAA‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTTAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGct                                                                                                              <  2:3644120/150‑1 (MQ=255)
                                         gctgctGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctct                                                                                                            >  2:4312892/1‑150 (MQ=255)
                                            gctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGt                                                                                                         >  1:5542977/1‑150 (MQ=255)
                                             ctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                        >  2:1968555/1‑150 (MQ=255)
                                              tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTaa                                                                                                       >  1:976826/1‑150 (MQ=255)
                                               gAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                      >  1:6770160/1‑150 (MQ=255)
                                                aaaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAACAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGa                                                                                                     <  2:4559742/150‑1 (MQ=255)
                                                 aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGGAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                    >  1:8015273/1‑150 (MQ=255)
                                                 aaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                    >  1:6111561/1‑150 (MQ=255)
                                                 aaaGGCAGCAGCTGATAACAAACCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGAAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGaa                                                                                                    <  2:4159399/150‑1 (MQ=255)
                                                  aaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                   >  2:3884137/1‑150 (MQ=255)
                                                  aaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                   >  1:6400880/1‑150 (MQ=255)
                                                  aaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAt                                                                                                   >  1:4805908/1‑150 (MQ=255)
                                                   aGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGATGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATg                                                                                                  >  1:3774637/1‑150 (MQ=255)
                                                     gcagcaGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTACGAATGCa                                                                                                >  2:7253966/1‑150 (MQ=255)
                                                        gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCg                                                                                             >  2:4236940/1‑150 (MQ=255)
                                                         cagcTGATAAGAAAGCAGCGGCAGAAAACGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGTCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGa                                                                                            <  1:929639/150‑1 (MQ=255)
                                                             tGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                        >  2:7660192/1‑150 (MQ=255)
                                                               aTAACAAAGCAGCGCAATAAAAAGCCGCCGAATAC‑AAAAAAGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgg                                                                                      <  2:2354680/150‑1 (MQ=255)
                                                                tAAGAAAGCAGCGGCAGAAAAAGCCGCCGCACAC‑AAAAAAGCGGCTGCGGAAAACGCTGCAGCTGAAAAAGCCGCTGCAGAAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggg                                                                                     <  2:3956172/150‑1 (MQ=255)
                                                                 aataacccagcGGCATAAAAAGCCGCCGCACAA‑AAAAAAGCGGCAGCGGAAAAAGCTGCAGCTGAAAAAGCCGATGAAGAAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                    <  1:1091727/143‑1 (MQ=255)
                                                                 aaGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AACAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                    <  2:2046874/150‑1 (MQ=255)
                                                                  aGAACGCAGCGGCAGAAACAGCCGCCGCAGACAAAAAAAGCGGCAGCGGCAAACGCTGCAGCTGAAAACGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggg                                                                                    <  2:7850873/150‑1 (MQ=255)
                                                                   gAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgggggg                                                                                  >  1:5909642/1‑150 (MQ=255)
                                                                    aaaGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTCAGACTGCCCCGAAAACGGGGGGa                                                                                 >  1:1415271/1‑150 (MQ=255)
                                                                        cagcGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgggg                                                                             >  2:2216262/1‑150 (MQ=255)
                                                                         cgcGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGc                                                                            <  2:5167063/149‑1 (MQ=255)
                                                                          gcGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCg                                                                           >  1:7251661/1‑150 (MQ=255)
                                                                             gCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaaa                                                                        >  2:3449721/1‑150 (MQ=255)
                                                                             gCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAGGCACCGAAAACGGGGGGAGGGGCGaaa                                                                        >  2:8083427/1‑150 (MQ=255)
                                                                                 aaaaaGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGa                                                                    >  1:5430370/1‑150 (MQ=255)
                                                                                 aaaaaGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGa                                                                    >  1:4473623/1‑150 (MQ=255)
                                                                                   aaaGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAAc                                                                  >  1:7894383/1‑150 (MQ=255)
                                                                                    aaGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACa                                                                 >  2:6690244/1‑150 (MQ=255)
                                                                                               gAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCt                                                      >  1:1760116/1‑150 (MQ=255)
                                                                                                   aaaaatGCGGACGCGGAAAAAGCTGCTGTTTAAAAAGCCCCTGCTGAAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGTTAAGAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   <  2:6249489/150‑1 (MQ=255)
                                                                                                   aaaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  2:6539398/1‑150 (MQ=255)
                                                                                                   aaaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  2:6954298/1‑150 (MQ=255)
                                                                                                     aaaaGCGGCAGCGGCAAACGCTGCAGCTGAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGAGGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCgg                                                 <  2:5310188/150‑1 (MQ=255)
                                                                                                     aaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCgg                                                 >  2:7797986/1‑150 (MQ=255)
                                                                                                      aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  1:3052224/1‑150 (MQ=255)
                                                                                                      aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  1:7648642/1‑150 (MQ=255)
                                                                                                      aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  1:6206608/1‑150 (MQ=255)
                                                                                                      aaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  2:3395995/1‑150 (MQ=255)
                                                                                                       aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGa                                               <  2:4722025/150‑1 (MQ=255)
                                                                                                        agcggcagcggcAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                              >  2:3816186/1‑150 (MQ=255)
                                                                                                          cggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTg                                            <  2:4496265/150‑1 (MQ=255)
                                                                                                           ggcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                           >  2:4083417/1‑150 (MQ=255)
                                                                                                            gcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGt                                          >  1:5230404/1‑150 (MQ=255)
                                                                                                            gcagcggaaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGt                                          <  2:3252320/150‑1 (MQ=255)
                                                                                                              agcggcaAACGCTGCAGCTGACAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTaa                                        <  2:4971483/150‑1 (MQ=255)
                                                                                                               gcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAAt                                       >  1:6644168/1‑150 (MQ=255)
                                                                                                                cggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATa                                      >  1:3843079/1‑150 (MQ=255)
                                                                                                                 ggcaACCGCTGCAGCTGAAAACGCCGCTGCAGCAAAC‑GCGGCCGCAGCGGCAGATGATATTTTCGGTGAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATAc                                     <  1:4152474/150‑1 (MQ=255)
                                                                                                                 ggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATAc                                     >  2:6146803/1‑150 (MQ=255)
                                                                                                                 ggaaaatGCTGCAGCTTAAAAACCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTTAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATAc                                     <  1:4958026/147‑1 (MQ=255)
                                                                                                                  gcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACt                                    >  2:1664499/1‑150 (MQ=255)
                                                                                                                  ccaAAAGCTCCAGCTAAAAACGCCGCGGCACAAAAG‑GCGGCCGCAGAGGCAGTTTTTATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACt                                    <  1:6935869/149‑1 (MQ=255)
                                                                                                                    aaaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                  >  1:2858058/1‑150 (MQ=255)
                                                                                                                     aaaGCTGCAGCTTAAAAAGCCGCTGCAGAAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 <  2:6358752/150‑1 (MQ=255)
                                                                                                                     aaaCCTCCAGCACAAAAACCCGCT‑CAACAAAACCCGGCCGCAGAGGCATATCATATTTTCGGTGAGCTAACCTCTGGTAACAATCCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 <  1:3934777/150‑1 (MQ=255)
                                                                                                                      acgCTGCAGCTTAAAAAGCCGCTGCAGCAAAC‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGTTAAGAATCCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaa                                <  1:4224896/148‑1 (MQ=255)
                                                                                                                           gCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAAt                           <  2:6655651/150‑1 (MQ=255)
                                                                                                                           gCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAAGGCACCGAAAACGGGGGGAGGGGCGACAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAAt                           <  1:4656563/150‑1 (MQ=255)
                                                                                                                             aGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                         >  2:3001239/1‑150 (MQ=255)
                                                                                                                             aGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                         >  1:1005997/1‑150 (MQ=255)
                                                                                                                              gattaaaaaCCCGATCCACCAAAC‑GCGGCCGCAGAGTCAGATGATATTTTCTGTGAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                        <  1:5640623/146‑1 (MQ=255)
                                                                                                                              gCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                        <  2:6449967/150‑1 (MQ=255)
                                                                                                                              gCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                        >  2:6824427/1‑150 (MQ=255)
                                                                                                                              gCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgc                        <  1:7462339/150‑1 (MQ=255)
                                                                                                                                  aaaaaGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                    >  2:3487560/1‑150 (MQ=255)
                                                                                                                                       gCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAggg               >  2:2667692/1‑150 (MQ=255)
                                                                                                                                          gcggcagcaAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc            <  2:2501325/147‑1 (MQ=255)
                                                                                                                                          gCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGcc            <  2:7020087/150‑1 (MQ=255)
                                                                                                                                           ccgcaccaAAC‑GCGCCCCCAGAGGCATATCTTATTTACGGTTAGCTAAGCCCTGGTAAGAATGCCACGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCg           <  1:2103812/148‑1 (MQ=255)
                                                                                                                                               acaaaac‑GCGGCCGCAGCGGCAGATGTTATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGatat       <  1:3528141/147‑1 (MQ=255)
                                                                                                                                                gcaAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATc      <  2:2754102/150‑1 (MQ=255)
                                                                                                                                                  aaaa‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa    >  2:5744163/1‑150 (MQ=255)
                                                                                                                                                  aaaa‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa    >  2:5493164/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGAC‑AAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAA‑GCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  NC_000913/777060‑777356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑