Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,983 A→T 5.3% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9830AT 5.3% 694.6 / 3.8 254intergenic (+457/+83)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base A (102/123);  new base T (5/12);  total (111/143)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

GGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACG  >  NC_000913/1286834‑1287131
                                                                                                                                                     |                                                                                                                                                    
 gCAGAATTTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                                                     <  1:693439/150‑1 (MQ=255)
 gCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                                                     <  1:7678279/150‑1 (MQ=255)
 gCAGAATATTTGATTGCGGATTAGTTTGAGAATTCCGGGGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAg                                                                                                                                                     <  1:629328/150‑1 (MQ=255)
  cAGAATATTTGATTGCGGATTCGTTTTAGATTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                                    <  1:6009775/150‑1 (MQ=255)
   aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTGGGGTCGTTGCGTGCGGGAACGGTGTGTCGGTGTCGCTGGAGGCGAAGCTTTGtg                                                                                                                                                   >  2:4308424/1‑149 (MQ=14)
   aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                                   >  1:4389379/1‑150 (MQ=255)
   aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                                   >  1:5438300/1‑150 (MQ=255)
       tatTTGAGGGCGGATGGGTTTGAGCATTCGGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                               <  2:6009602/150‑1 (MQ=14)
           tGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                                           <  1:5912876/150‑1 (MQ=255)
             aTTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtctc                                                                                                                                         <  1:3853072/150‑1 (MQ=255)
                    aTTCGTTAGAGTATTCCTGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                                  <  1:3158709/150‑1 (MQ=255)
                      tCGTTTGAGAATTCCGGGGCTTTTGACAGTGAGGGTGGTGGGGGTTGGTTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                                                <  1:2060397/150‑1 (MQ=14)
                      tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGACCTTCGCCCTTCGGGTCGATGCCGTCCGCAAAGCTCTCACCCTGACTCTCGAGCCGAACCTAACTCGAAGCTACACATccaaaac                                                                                                                                >  2:5073861/1‑145 (MQ=14)
                        gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                              <  2:4137824/150‑1 (MQ=255)
                        gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCAACCCGCTCACCCTGACCCTCGAGCCGCACCATAGTCGAAGCTTCTCAACCGAccccc                                                                                                                              >  1:8227768/1‑149 (MQ=14)
                         tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                                             >  2:7938848/1‑150 (MQ=255)
                         tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCACATCCAACCCCGc                                                                                                                             >  2:4824211/1‑150 (MQ=255)
                            gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                                                          >  2:7511369/1‑150 (MQ=255)
                            gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCGTAGTCGAAGCTACACATCCTTCCCCCCATg                                                                                                                          >  2:6987534/1‑150 (MQ=11)
                              gaATTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                                        <  2:1070711/150‑1 (MQ=255)
                               aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                                       <  2:7778760/150‑1 (MQ=255)
                                        gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                              <  2:5102885/150‑1 (MQ=38)
                                        gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAAttt                                                                                                              >  2:3476903/1‑150 (MQ=38)
                                          ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGTCGCGCGAGCCGAACCATAGTCGAAGCATAAAAACCATCCCCCCATGGGCAGAATAttttt                                                                                                            >  1:7563390/1‑148 (MQ=11)
                                               aacgTGATGGGGGTGGGGGACGGATTACTCCGCGCGGCGCGCTTCGCCCTTCGGTTCGTGGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCg                                                                                                       <  2:6762546/147‑1 (MQ=14)
                                                   cgATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAtt                                                                                                   <  2:1873519/149‑1 (MQ=39)
                                                    gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTc                                                                                                  <  1:6166562/150‑1 (MQ=39)
                                                    gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTc                                                                                                  <  2:154468/150‑1 (MQ=39)
                                                        gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                                              <  1:2825580/150‑1 (MQ=255)
                                                           gtgctgtaaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCAAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                                           <  1:2243612/143‑1 (MQ=255)
                                                              ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGt                                                                                        >  2:7330340/1‑150 (MQ=255)
                                                                 gAAGGATTACTCAGCGCGGCGCGCTTCGCCCTGCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                                                                     <  2:8227768/150‑1 (MQ=255)
                                                                  aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                                    >  2:6391542/1‑150 (MQ=255)
                                                                  aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                                    <  2:905364/150‑1 (MQ=255)
                                                                  aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                                    <  1:6463860/150‑1 (MQ=255)
                                                                  aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                                    <  2:4457283/150‑1 (MQ=255)
                                                                   aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                                                   <  1:1864772/150‑1 (MQ=255)
                                                                   aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                                                   <  2:1987149/150‑1 (MQ=255)
                                                                      aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                                                <  1:5237983/150‑1 (MQ=255)
                                                                      aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                                                <  2:2338199/150‑1 (MQ=255)
                                                                      aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCTCTCGCGCGGACGCGCGAGCCGAACCTGAGTCGAAGCGTCTCATACGTCCCCGCCAGGGCAGGAGATATGATAGCGGATTCGCTTGGGAGttgcgggcc                                                                                >  2:7618462/1‑143 (MQ=14)
                                                                       ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACATTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACt                                                                               <  1:3397843/150‑1 (MQ=255)
                                                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATCGTTCATTGCGGATTCGCTTGGGAGTTCAGGGACttgt                                                                            >  2:6023271/1‑148 (MQ=14)
                                                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                                            <  1:4143793/150‑1 (MQ=255)
                                                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCAGGGGCCGAATATTTGATTGCGGATTCGCTGGAGAGTTCAGGGAcggtt                                                                            >  2:3032853/1‑146 (MQ=14)
                                                                          cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCGCGCTGACGCGCGAGCCGAACCGTAGTCGAAGCGTCTCATCCTTCCCCGCAAGGGCCGAAGATTTGATTGCGGATTCGCGGGGGAGTTCAGGGACtttt                                                                            >  2:959818/1‑150 (MQ=14)
                                                                           tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGCACCGTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGTTCAGGGACGTTTg                                                                           >  2:375047/1‑150 (MQ=11)
                                                                           tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGCAGCTTCTCATCCTTCCCCGCATGGGCCGAATAGTTGATTGCGGATGCGCTTGTGAGGTCCGGGAcgtgtg                                                                           >  2:7856687/1‑145 (MQ=14)
                                                                             aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                                         <  1:2362842/150‑1 (MQ=255)
                                                                             aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTTGGGAGTTCAGGGACTTTTGaa                                                                         >  2:6184295/1‑150 (MQ=255)
                                                                              ggggtGCGCTCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                                        <  1:963880/146‑1 (MQ=255)
                                                                                   gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAT                                                                   <  2:77673‑M1/150‑1 (MQ=255)
                                                                                   gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAT                                                                   <  2:7240160‑M1/150‑1 (MQ=255)
                                                                                        cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGG                                                              >  2:6809208‑M1/1‑150 (MQ=255)
                                                                                         ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGTAGTTCAGGGACTGTTGAAAGTGATGGGGGT                                                             >  2:7553911‑M1/1‑150 (MQ=255)
                                                                                         ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGT                                                             >  2:6852709‑M1/1‑150 (MQ=255)
                                                                                         ttCGCCCTTCGGGTCGTTGCCGGCCGCAACGCTCACACGCAGACGCACGAGCCGAACCATAGTCGAATCATCTCAACAATAACCCCAATGGCCGAAAATTTGATTTAGGATTCGCTTGAGAGTTAAGGGCATTTTGTCAGAGAtgggggg                                                             >  2:2976174/1‑149 (MQ=12)
                                                                                            gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGG                                                          <  2:173538‑M1/150‑1 (MQ=255)
                                                                                            gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGTACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGG                                                          <  1:7568604‑M1/150‑1 (MQ=255)
                                                                                              ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCATTGACTTTTGAAAGTGATGGTGGTGGGGG                                                        <  1:3613279‑M1/150‑1 (MQ=255)
                                                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                                      <  2:2948271‑M1/150‑1 (MQ=255)
                                                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                                      <  1:7369185‑M1/150‑1 (MQ=255)
                                                                                                 tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                                                     <  1:2570165‑M1/150‑1 (MQ=255)
                                                                                                 tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                                                     <  1:4767926‑M1/150‑1 (MQ=255)
                                                                                                 tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                                                     <  1:7964632‑M1/150‑1 (MQ=255)
                                                                                                 tCGGGTCGTTGACAGCGGCAACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGACTAATTGATTGCGGATTCGCTTGAGAGTTCACGGACTTTTGAAAGTGTGGGTTGTGGGGGAAG                                                     >  2:4783141‑M1/1‑150 (MQ=255)
                                                                                                    ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                  <  1:7308871‑M1/150‑1 (MQ=255)
                                                                                                    ggTCGTTGCCGGCGGCAACGCTCTCTCCCTGACGCTCGGGCCGAACCGTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                  >  2:5263378‑M1/1‑150 (MQ=255)
                                                                                                    gagcgttGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                                  >  2:5563162‑M1/4‑150 (MQ=255)
                                                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAACGTGATGGTGGTGGGGGAAGGATTACt                                              >  2:3129661/1‑150 (MQ=255)
                                                                                                        gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTCATTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTaga                                              >  2:2593948/1‑148 (MQ=255)
                                                                                                          tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                                            <  1:5058417/150‑1 (MQ=255)
                                                                                                           gCCTGCGGCACCGCTCTCCCGCAGACGCTAGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAg                                           <  2:4200770/150‑1 (MQ=17)
                                                                                                            ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTGATTGCGGATTAGCGTGATAGTTCAGGGACATTTGAAAGTGATGGGGGTGGGGGGAGGATTAATCAgc                                          >  1:8073684/1‑150 (MQ=17)
                                                                                                              tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                        >  2:5354074/1‑150 (MQ=255)
                                                                                                                       gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCtt                               >  2:2634156/1‑150 (MQ=255)
                                                                                                                        ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                              >  2:6072695/1‑150 (MQ=255)
                                                                                                                        ctctctCGCTGACCCTCGAGCCGAACCATAGTCGAAGCGTCTCATCCATCACCCCAGGGGCAGAATATTTGATTTCGGATTCGATTGAGAGTTCAGGGACTTTTTAAATTGATGGTGGTGGGGGAAGGATTACTAACCGCGGCGCGCtta                              >  1:3655191/1‑149 (MQ=17)
                                                                                                                         tctctcGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                             <  1:5834981/150‑1 (MQ=255)
                                                                                                                         tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                             >  1:1196716/1‑150 (MQ=255)
                                                                                                                         tctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                             <  1:2976174/150‑1 (MQ=255)
                                                                                                                           ccgcGCTGCCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGGTTTGGCAGTTTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTATTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc                           <  1:2170824/147‑1 (MQ=14)
                                                                                                                              cGCTGGCGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                        <  1:5354074/150‑1 (MQ=255)
                                                                                                                               gCTGTCGCTCTAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                       <  2:541489/150‑1 (MQ=255)
                                                                                                                               gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCCCTTCGCCCttg                       >  2:8122767/1‑149 (MQ=255)
                                                                                                                                cccccGCTCGATGCGAACGTGAGGCGTAGCTCCTATTCCTTCCCCGCTTGGGCACCTTATTTGATTGCGGACTCGCTTGAGAGCTCTGTTACTTTTGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg                      <  2:6491415/146‑1 (MQ=2)
                                                                                                                                 tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg                     <  1:4502080/150‑1 (MQ=255)
                                                                                                                                  gACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg                    >  2:8057411/1‑150 (MQ=255)
                                                                                                                                   aCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCACCGCGGCGCGCTTCGCACGTCGGGt                   >  2:3429208/1‑150 (MQ=255)
                                                                                                                                    cGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                  <  1:6813542/150‑1 (MQ=255)
                                                                                                                                      cTCGCGCCGCCCCGTAGTCGCCGCTTCTCCTCGGTCGCCGGATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGt                <  2:5720942/150‑1 (MQ=14)
                                                                                                                                       tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGtg               >  1:5665746/1‑149 (MQ=255)
                                                                                                                                       tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCATCGGGtagat               >  1:8156015/1‑146 (MQ=255)
                                                                                                                                       tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCCCTTCTCCGATCGGGTCgat               >  2:6251498/1‑148 (MQ=17)
                                                                                                                                         gAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc             <  1:1595904/150‑1 (MQ=255)
                                                                                                                                          aGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGcc            >  1:1013207/1‑150 (MQ=255)
                                                                                                                                               caCCTGAGTCGACGCTTCTCAGCCGTCCCCGCCTGGGCCGAAGATTTGATTGCGGATGCGCTTGAGAGTTCAGGGACTTTTGCACGTGATGGGGGTGGGGGAAGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGccgcagg       <  1:4824211/149‑6 (MQ=14)
                                                                                                                                               aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg       >  2:6455721/1‑150 (MQ=255)
                                                                                                                                                  cTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa    >  1:4606033/1‑150 (MQ=255)
                                                                                                                                                    tAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACg  >  2:6057742/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACG  >  NC_000913/1286834‑1287131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: