Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 6.0% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 6.0% 718.2 / 24.3 255noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (121/114);  new base A (8/7);  total (134/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298
                                                                                                                                                    |                                                                                                                                                     
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      >  2:5067932/1‑150 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      <  2:4673996/150‑1 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCgc                                                                                                                                                      >  1:1569706/1‑149 (MQ=255)
   cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                   >  1:2778333/1‑150 (MQ=255)
    ccGCGTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                                  >  1:5993423/1‑150 (MQ=255)
      gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                >  2:7889611/1‑150 (MQ=255)
      gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                >  2:5100239/1‑150 (MQ=255)
        aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAcc                                                                                                                                              <  2:438646/150‑1 (MQ=255)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                          >  1:1049158/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTAGCATTCGCGCCAGTCGAAccgttgt                                                                                                                                         >  1:3641753/1‑145 (MQ=14)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                        >  1:5505159/1‑150 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAAAGCACTCTAGCTGGCGCTCGAGTCGAACCCTGGTCg                                                                                                                                       >  1:771388/1‑150 (MQ=14)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGGAACGCTCTCTCGGTGGCGGTGGTGTGGAACCTTGGTCGTAGCt                                                                                                                                  >  2:1524442/1‑150 (MQ=14)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCt                                                                                                                                  >  2:7520066/1‑150 (MQ=255)
                          tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACACGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCGTCTCaa                                                                                                                            >  1:7370308/1‑149 (MQ=14)
                           gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGCTTGCGCTCGATTCGAACCTTGGTCGAATGTTCTCaac                                                                                                                           >  2:5704675/1‑148 (MQ=14)
                               aTTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGGAACGCTGTCTCGCTGGCGCGCGAGTCGAACCGTGGTAGAATCTTCTCATACTTc                                                                                                                       >  2:6956071/1‑150 (MQ=14)
                                 tCGCTTGAGAGTTCAGGGACCTTTGAAAGTGATGGTGGTGGGGGAAGGATTTCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                                     <  1:5833484/150‑1 (MQ=255)
                                         gacttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                             <  2:6030157/147‑1 (MQ=255)
                                            ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCACTCACGCGGGCGCGCGAGTCGAACCATGGTCCAAGCATCACAACCATCCCCCCCGGGGCAg                                                                                                          >  1:6091939/1‑150 (MQ=14)
                                              ccgggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGATTCGAACCTTAGTCGAAGGTTCTCAGCCTTCCCCGCAGTGGCCGaa                                                                                                        >  2:5408754/3‑150 (MQ=11)
                                               aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCGGCGGCCACGCGCTCTCGCTGGCGCGCGAGGGGAACCGTGGTCGGAGCGTCTCAAACATCCCCGCAAGGGCCGAat                                                                                                       >  1:6245027/1‑150 (MQ=11)
                                               aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                       <  2:5543237/150‑1 (MQ=40)
                                                gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACCCACACACGCGGGCGCTCGAGTCGCACCGTGGTCGAATCTTATAAAACATACCCCCAGGGGCAGAata                                                                                                      >  1:2564961/1‑150 (MQ=14)
                                                 ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTAGGTCGAAGCTTCTCATCCATCCCCGCAGGGGCAGAatag                                                                                                     >  2:5094431/1‑149 (MQ=255)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGACTCGAACCATGGTCGAAGCTTCTCACCCTTCCCCGCAGCGGCACAACATTaaa                                                                                                 >  2:5372084/1‑150 (MQ=11)
                                                        tGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAACCTCACACGCTGGCGCTCGAGTAGAACCATTGTCGAAACTTAACAACCATACCCCCAAGGGCAGAGGATTTAATTg                                                                                              >  2:3655985/1‑150 (MQ=11)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTATCATACATACCCGCAAGGGCCGAATATTTAATTGCg                                                                                            >  2:2334858/1‑150 (MQ=14)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTATCGCTGGCGCTCGCGTCGAAACAGTGTCGAATCTTCTCATCCTTACCCCCAGGGGCAGAATATTTAATTTCg                                                                                            >  2:2877765/1‑150 (MQ=11)
                                                              tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACACGCAGGCGCTCGAGTCGAACCTTGGTCGAAACTTATCAAACATACCCCCATGGGCACAATATTTAATTGCGGAtt                                                                                        >  2:5737040/1‑150 (MQ=11)
                                                                   gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                   <  1:7938848/150‑1 (MQ=38)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACCCACGCACCCGGGCGCGCCGGTCGAACCGTGGTCGGAGCATATCAACAAACCCCCCAAGGGCCGAAAATTTTATTTCGGGttgggggg                                                                                  >  1:487734/1‑144 (MQ=2)
                                                                       tggGGGCAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCATCGCTCTCTCGCTGGGGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               <  1:7230380/150‑1 (MQ=255)
                                                                       tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               <  1:6443243/150‑1 (MQ=40)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                              <  1:4693848/101‑1 (MQ=32)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGTGAAGTTc                                                                           >  2:5876489/1‑150 (MQ=255)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:6987534/150‑1 (MQ=255)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:7511391/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCGCGCTGGCGCACGAGACGAGCCGTGGTCGAAGCGTCTCATCCATCCCCGCCTGGGCCGAATATTTTATTGCGGGGTCGGTGGGCAGTTCAgggg                                                                      >  2:3746408/1‑149 (MQ=11)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCTCTCGCGGGCGCTCGAGGCGAACCGTGGTCGCAGCGTCTCATCCATCCCCCCCGGGGCCGAATAGTTAATTGCGGCGTCGTTGGGGAGTTCAgggc                                                                      >  2:7619726/1‑149 (MQ=14)
                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                    <  1:3116915/150‑1 (MQ=255)
                                                                                    aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCTGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  1:684065/150‑1 (MQ=255)
                                                                                    aCTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATAGGCAGAATATTTAATGGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  2:1013207/150‑1 (MQ=255)
                                                                                    aCTCAGCGCGGCGCGCTTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  1:8193264/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                 <  1:3851701/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                >  2:1897758/1‑150 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCGTCTCATCCGTCCCCGCCGGGGCCGACTAGGTAATTGCGGCTTCGGGGGGCAGTTCAGGGAcggtgg                                                                >  2:4068577/1‑145 (MQ=14)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCGGCGGCAACGCGCGCTAGCTGGCGCTCGACTCGGACCGAAGTCGAAGCGTCTCATCCGTCCCCGCCAGGGCAGAAGATTTACTTGGGGATTCCGTGGGTAGTTCAgggcaagttg                                                                >  2:3019312/1‑143 (MQ=9)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 >  2:2671471/1‑115 (MQ=38)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  2:1735938/150‑1 (MQ=255)
                                                                                                gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGCAGTTCAGGGACTTTTGAAAGTGAtgg                                                      >  2:6051396/1‑150 (MQ=11)
                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtg                                                    <  1:5067932/150‑1 (MQ=255)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCCCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTTTtggtggt                                                  >  2:5824600/1‑150 (MQ=255)
                                                                                                       gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                               <  2:5691854/150‑1 (MQ=255)
                                                                                                       gCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                               <  1:5746485/150‑1 (MQ=255)
                                                                                                        cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTg                                                          <  1:615784/138‑1 (MQ=255)
                                                                                                         ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                             <  1:5364247/150‑1 (MQ=255)
                                                                                                              gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCGCGGATGGGCATAATATTTTTTGGCGGATTCTTTTGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGATGGa                                        <  1:5566606/150‑1 (MQ=14)
                                                                                                               gagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAGAGTGATGGTGGTGGGGGAAGGAt                                       >  2:7707554/4‑150 (MQ=17)
                                                                                                                agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTAATTGCGGCTTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      >  1:7442051/3‑150 (MQ=17)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  1:6729587/150‑1 (MQ=255)
                                                                                                                  cGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCg                                    <  1:2946333/150‑1 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  1:2087454/98‑1 (MQ=18)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       >  2:2087454/1‑98 (MQ=18)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 >  1:3322218/1‑87 (MQ=40)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 <  2:3322218/87‑1 (MQ=40)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAAACCTTCCCCCCCTGGGCAGAATATTTAATTGCGGATTCGGTGGGTAGTTAAGGGACGTTTGGAAGGGATGGTGGGGGGGGGAGGATTAGaa                                  >  1:4274142/1‑150 (MQ=14)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:1321829/1‑150 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:6956071/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             >  2:6440134/1‑150 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATAGCGGATACGTGGGGAAGGACAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGTATACGAACCTTc                             >  2:3215475/1‑150 (MQ=17)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                       >  2:7259532/1‑123 (MQ=255)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAt                                                        >  2:3577839/1‑121 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          <  1:2436909/150‑1 (MQ=255)
                                                                                                                              cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAAGTGCAGAGTTTTTTATTGCTGATTAGTTGTGTAGTAAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                        <  1:3473293/150‑1 (MQ=17)
                                                                                                                                aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                      >  2:6676847/1‑150 (MQ=255)
                                                                                                                                 cGCTCTCTCGCTGTCGCTCGATTCGAACCATGGTCGATTATTCTCATCCTTCCCAGAATGGGCAGAATATTTAATTGCGGATTATATTGTTACTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     <  2:7130494/150‑1 (MQ=17)
                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggt                                                     <  1:6049600/117‑1 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:4876686/1‑150 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  <  1:2382584/150‑1 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:2516001/1‑150 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:1544860/1‑150 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGGCGATGACg                >  2:2555356/1‑150 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATCGGCAGAACATTCAAAGGCCGCTACGGTCGGCAGTTCACGGACGTTTGACAGTGGTGGGGGTCGGGGACGGATTCGAACCGTCGAAGACGATGACg                >  2:3880988/1‑150 (MQ=16)
                                                                                                                                      tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:20733/93‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg               <  1:3746408/150‑1 (MQ=255)
                                                                                                                                       ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg               >  2:890654/1‑150 (MQ=255)
                                                                                                                                         cGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa             <  1:1766857/150‑1 (MQ=255)
                                                                                                                                          gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGtt                                                                            <  1:1258077/86‑1 (MQ=255)
                                                                                                                                           cTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa           <  1:4881078/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa           <  2:6861693/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa           <  1:5073861/150‑1 (MQ=255)
                                                                                                                                              gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt        <  1:4179800/150‑1 (MQ=255)
                                                                                                                                               cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa       <  1:6391542/150‑1 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      <  2:3088261/150‑1 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      >  2:2037608/1‑150 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      >  2:8110112/1‑150 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:2586711/150‑1 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  2:4406837/1‑150 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  2:430453/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: