Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,507 G→T 7.1% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5070GT 7.1% 1055.4 / 10.3 339S77* (TCA→TAA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (188/113);  new base T (12/25);  total (200/139)
Fisher's exact test for biased strand distribution p-value = 6.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656
                                                                                                                                                     |                                                                                                                                                     
 gCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGa                                                                                                                                                      <  1:7949617/150‑1 (MQ=255)
  cAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAt                                                                                                                                                     <  1:6010520/150‑1 (MQ=255)
     gggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtttt                                                                                                                                                  >  2:2456808/1‑150 (MQ=255)
      ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 <  2:1687042/150‑1 (MQ=255)
       gTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttat                                                                                                                                                >  2:8229299/1‑150 (MQ=255)
          aTAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTtattat                                                                                                                                             >  2:7559947/1‑150 (MQ=255)
           tAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATc                                                                                                                                            <  2:583506/150‑1 (MQ=255)
            aaTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCg                                                                                                                                           <  1:3095037/150‑1 (MQ=255)
              ttAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAgta                                                                                                                                         >  1:8122836/1‑150 (MQ=255)
               tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  2:6712015/150‑1 (MQ=255)
                aGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaag                                                                                                                                       <  1:3191236/150‑1 (MQ=255)
                  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                     >  2:4867112/1‑150 (MQ=255)
                    ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                   >  2:5350008/1‑150 (MQ=255)
                         atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTaa                                                                                                                              >  2:4280925/1‑149 (MQ=255)
                          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  1:6733826/150‑1 (MQ=255)
                           atCCTGTGACAAAAGGAGCTACTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAt                                                                                                                            >  1:2634394/1‑150 (MQ=255)
                              cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                         >  2:5239094/1‑150 (MQ=255)
                               tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  2:379256/1‑150 (MQ=255)
                                gtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAata                                                                                                                       <  2:5977989/150‑1 (MQ=255)
                                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                    <  1:4742353/150‑1 (MQ=255)
                                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                    >  2:3458559/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  2:2002057/1‑150 (MQ=255)
                                        aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                               <  1:1403733/150‑1 (MQ=255)
                                        aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                               <  2:659233/150‑1 (MQ=255)
                                         ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaat                                                                                                              >  2:6317647/1‑149 (MQ=255)
                                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                             <  2:2807798/150‑1 (MQ=255)
                                           aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                            <  2:6786686/150‑1 (MQ=255)
                                            gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                           <  2:616279/150‑1 (MQ=255)
                                            gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                           >  1:962837/1‑150 (MQ=255)
                                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTTTATTAATTAAAATTAAAATAAAATTTaaaatttaaaa                                                                                                         >  1:3749416/1‑143 (MQ=255)
                                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                         <  1:6302273/150‑1 (MQ=255)
                                                ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACg                                                                                                       >  1:82728/1‑150 (MQ=255)
                                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTATGTATGATTTTATTTTCGTAAGTAAAATTAGATGTTTATTTTATATGGTAAACGTCATgt                                                                                                 >  1:2313607/1‑149 (MQ=255)
                                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                 >  2:6442630/1‑150 (MQ=255)
                                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAATTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGa                                                                                                 >  2:5297579/1‑150 (MQ=255)
                                                      gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                 >  1:244411/1‑150 (MQ=255)
                                                        gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                               >  1:1070381/1‑150 (MQ=255)
                                                         cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                              >  1:1303184/1‑150 (MQ=255)
                                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                             >  1:5640538/1‑150 (MQ=255)
                                                          tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                             >  1:5779553/1‑150 (MQ=255)
                                                           aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                            >  1:7367250/1‑150 (MQ=255)
                                                           aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAACg                                                                                            >  1:5243716/1‑150 (MQ=255)
                                                            ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTTTATTTTTATGTCTGTGGCGAAATTGAATACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                           <  2:7814155/150‑1 (MQ=255)
                                                            ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                           <  2:7216243/150‑1 (MQ=255)
                                                            ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                           >  2:1423300/1‑150 (MQ=255)
                                                                      tATTAGTAAAAATATAAGAGTTAGATGAATTTTTATGTCTGTGGCGAAATTGACTACCAACGTATTGTTGTTTAAGAATGAATTTAATATCGTAAATAGAAGTAAATGTCTATTTAAAAATGAAAAACAAAAGAAAACGAATCACAGaaa                                                                                 >  2:6991029/1‑149 (MQ=255)
                                                                       aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGTAAAAAGACATGAAACCGAAGCACAGAATc                                                                                >  1:994270/1‑150 (MQ=255)
                                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                           >  2:5461614/1‑150 (MQ=255)
                                                                               aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATGAAGAATTATTTTATAATCGAAACTAAAATTACAATAATAATTAAAAAGGAAAACCACAATAAAACCAAGCACATAATCAAAAttat                                                                        >  1:2714876/1‑148 (MQ=255)
                                                                                aaTATAAGCGTTAGGTTTTTTGTTTTGACTTTGGCTAATTTGACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCGTATTTAAATTTACATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  1:2053031/150‑1 (MQ=255)
                                                                                 atatAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAATTTACATGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTcc                                                                      <  2:7864872/150‑1 (MQ=255)
                                                                                  tataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAATTAAAAATACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                     >  1:7299653/1‑150 (MQ=255)
                                                                                   ttaATAGTTCGTTTTATTTTTATTTCTGTGGCCAATTTCACTACCTTCTTTTTTTTTATTAATAATTATTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                    <  1:5978592/149‑1 (MQ=255)
                                                                                   ataAGTGTTTGCTTTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTTATTAATAATTTTTTTATTATCTTAATTAAAATTAAATTAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                    <  1:1217291/150‑1 (MQ=255)
                                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAATCGACATGAAACCGAAGCACAGAATCAACATTATCCAAt                                                                   >  2:1699310/1‑150 (MQ=255)
                                                                                     aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                  >  2:1412848/1‑150 (MQ=255)
                                                                                     aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATGCTCCAATc                                                                  >  2:7168575/1‑150 (MQ=255)
                                                                                        agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATa                                                               <  2:4535730/150‑1 (MQ=255)
                                                                                           tAGATGTTTTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaaa                                                            <  1:6490436/150‑1 (MQ=255)
                                                                                                  aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTATCCAATCATAAAATATTTcc                                                     >  2:113425/1‑150 (MQ=255)
                                                                                                   ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGAAATGAAACCCAAGCACAGAAAAAAAAATATACAAAAATAAAAAATTTCCg                                                    >  1:5890370/1‑150 (MQ=255)
                                                                                                    tGTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                   <  1:5184805/150‑1 (MQ=255)
                                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  <  2:2087575/150‑1 (MQ=255)
                                                                                                      ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTTAGAATGATTTTATTATCGTAAGTAAAATTAAAAAAAAATTTAAAAATGAAAAAGACATGAAAACGAAGCAAAAAATAAACAATATACAAAAATAAAATAATTACGttg                                                 >  1:4492605/1‑148 (MQ=255)
                                                                                                      ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGTATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAATCGTCATGAAACCGAAGCTCAGAATCAAGATTCTGCTATCATATAATTTTTGGGTgg                                                 >  2:4374835/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGTGGAGCAttt                                          >  2:2963627/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                          >  2:3967024/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAATATTTTATTATAGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAACGAAAATAAAACCAAGCACAGAAACAACAAAATCCAAAAAAAAAAAATTTAAGTGGAGCAttt                                          >  1:1020761/1‑150 (MQ=255)
                                                                                                               tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                        >  2:6019811/1‑150 (MQ=255)
                                                                                                               tgGCGAAATTGACTACCTTCGTGTTTTTGTTTAATAATTTTTTTATTATAGTAAGTAAAAATAAAAGAATATTTTAAAAGGAAAACCACAAGAAAACCCAGCACACAAAAAACAATATCCAAAAATAAAAAATTTACGTGGAGCATTtta                                        >  1:3905064/1‑150 (MQ=255)
                                                                                                                 gagAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      <  2:3399541/148‑1 (MQ=255)
                                                                                                                   gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                    <  2:4785539/150‑1 (MQ=255)
                                                                                                                    aaaTTTACTAACTTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  2:7382854/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:1988270/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:6502487/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  2:4169259/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  2:7338339/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTGTCTTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGCAGCACAGAATCAACATTCTCCAATCATACAATATTTCCGTGGAGCATTTTattatt                                   <  2:8086119/150‑1 (MQ=255)
                                                                                                                    aaaTTGACTACCTACGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  2:4671384/150‑1 (MQ=255)
                                                                                                                               cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                        >  2:1599152/1‑150 (MQ=255)
                                                                                                                               cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                        >  2:2370452/1‑150 (MQ=255)
                                                                                                                               cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                        >  2:2559453/1‑150 (MQ=255)
                                                                                                                                ttCTTTTTTTTGATTAAGAATGTTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                       <  1:1621611/150‑1 (MQ=255)
                                                                                                                                ttCGTTTTTTTGATTAATAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                       <  1:5042811/150‑1 (MQ=255)
                                                                                                                                 tCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAAAATTTCCGTGGAGCATTTTATTATTGAATATATAGGtt                      >  1:1901228/1‑150 (MQ=255)
                                                                                                                                  tgTTTTTTTTAATATGATTTATTTTATTTGCGTAATTAAAATTACTTGATTTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                     <  1:1689903/149‑1 (MQ=255)
                                                                                                                                  cGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                     <  2:263498/150‑1 (MQ=255)
                                                                                                                                    tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAACAACAATCTCCAATCATAAAATATTTCCGTGGAGCAATTTATTATTGAATATAGAGGTTTaa                   >  2:5134737/1‑150 (MQ=255)
                                                                                                                                       ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                >  2:7750351/1‑150 (MQ=255)
                                                                                                                                        tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc               <  1:7559947/150‑1 (MQ=255)
                                                                                                                                         tttaTTAATAATTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              <  1:4221948/147‑1 (MQ=255)
                                                                                                                                         ttGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              <  2:6002787/150‑1 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGTTTTAAGTCAGGTa           >  1:2736857/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  1:5366440/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  1:3587858/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATAATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTCACTCCGGtc           >  1:4981785/1‑149 (MQ=255)
                                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCTGGTaaa         >  1:6985995/1‑150 (MQ=255)
                                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa         >  1:6849935/1‑150 (MQ=255)
                                                                                                                                                 taaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  2:3150212/149‑1 (MQ=255)
                                                                                                                                                  aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     >  2:3588692/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: