Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,228 C→A 11.0% intergenic (‑24/+284) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2280CA11.0% 901.9 / 13.6 348intergenic (‑24/+284)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (110/181);  new base A (51/2);  total (164/184)
Fisher's exact test for biased strand distribution p-value = 1.03e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377
                                                                                                                                                     |                                                                                                                                                     
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  2:4230430/150‑1 (MQ=255)
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  1:5878638/150‑1 (MQ=255)
aTGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTc                                                                                                                                                       <  1:4322793/150‑1 (MQ=255)
 tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCg                                                                                                                                                      <  2:7988216/150‑1 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGa                                                                                                                                                     >  1:5316160/1‑150 (MQ=255)
  gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                     <  2:729032/150‑1 (MQ=255)
   cAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAt                                                                                                                                                    <  2:4484053/150‑1 (MQ=255)
     ggTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAttt                                                                                                                                                  >  1:6394505/1‑150 (MQ=255)
      gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                 <  2:3893662/150‑1 (MQ=255)
      gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAttta                                                                                                                                                 >  2:2859035/1‑149 (MQ=255)
        tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                               <  1:7655357/150‑1 (MQ=255)
         ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCTCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                              <  1:6956401/150‑1 (MQ=255)
         ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                              <  1:3154656/150‑1 (MQ=255)
          gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                             <  2:1719263/150‑1 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                           >  1:5463423/1‑150 (MQ=255)
            aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                           >  1:3467613/1‑150 (MQ=255)
             gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                          >  2:6213112/1‑150 (MQ=255)
                  ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAtta                                                                                                                                     >  2:3706228/1‑148 (MQ=255)
                  ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                     <  1:7954150/150‑1 (MQ=255)
                  ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAtta                                                                                                                                     >  1:5926868/1‑148 (MQ=255)
                   tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                    <  2:7593689/150‑1 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGaaa                                                                                                                                   >  1:2947416/1‑150 (MQ=255)
                    cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTaaa                                                                                                                                   >  1:1880384/1‑150 (MQ=255)
                      accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCCATTTCAAAAAATAATGaaaat                                                                                                                                 >  2:7187451/1‑148 (MQ=255)
                         accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAAAATGAAattatt                                                                                                                              >  2:2567815/1‑150 (MQ=255)
                               ttcATCGTTTCGAGATTGTGAATATTGAATGCCTGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTAtttttttt                                                                                                                        <  2:233151/148‑1 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                       <  2:2961731/150‑1 (MQ=255)
                                gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATtttttttt                                                                                                                       >  1:7800556/1‑150 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTa                                                                                                                      >  1:2306809/1‑150 (MQ=255)
                                 cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAAAAATAAATTATTTGTTTTTa                                                                                                                      >  1:663735/1‑150 (MQ=255)
                                   tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTaaa                                                                                                                    >  1:938575/1‑150 (MQ=255)
                                      tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTGTTTTTAAatat                                                                                                                 >  1:27441/1‑150 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                              <  2:4890806/150‑1 (MQ=255)
                                         cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                              <  1:7056269/150‑1 (MQ=255)
                                           agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCgaga                                                                                                            >  1:4489473/1‑150 (MQ=255)
                                            gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                           <  2:7906879/150‑1 (MQ=255)
                                                gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                       <  2:4556912/150‑1 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                      <  1:2647270/150‑1 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                      <  2:6900093/150‑1 (MQ=255)
                                                 tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGATTTTAAATATCGAGATAACGa                                                                                                      <  2:5053481/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                     <  2:6069470/150‑1 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGTTTTGAAAAAATAATGAAATTTTATTTTTTTAAATATAGAGATAACGAt                                                                                                     >  1:1537943/1‑150 (MQ=255)
                                                  gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGTTAACGAt                                                                                                     >  1:2347742/1‑150 (MQ=255)
                                                   aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                    <  1:3701692/150‑1 (MQ=255)
                                                     tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTAAAAATAGAGATAAAGATaac                                                                                                  >  1:1410462/1‑150 (MQ=255)
                                                      atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                 <  2:5583626/150‑1 (MQ=255)
                                                       ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACaa                                                                                                <  2:5839726/150‑1 (MQ=255)
                                                         gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                              >  2:7480151/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                             >  2:2036816/1‑150 (MQ=255)
                                                          aaTGCCGGAACCGCATAACAGCCGCGCTGTGAGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAAAGAAAACacaaa                                                                                             >  2:7819155/1‑150 (MQ=255)
                                                           aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAACGATAACaaaaaa                                                                                            >  2:5701424/1‑149 (MQ=255)
                                                             gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGa                                                                                          >  1:7521072/1‑150 (MQ=255)
                                                              ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                         <  2:2216552/150‑1 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACa                                                                                        >  2:2702000/1‑150 (MQ=255)
                                                               cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTCAAAATAAAAAGAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACa                                                                                        >  2:3687632/1‑150 (MQ=255)
                                                                    ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCAAAAAAACGACAatatt                                                                                   >  2:4269483/1‑149 (MQ=255)
                                                                          aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAAAGACAAAATGAAAatt                                                                             >  2:6992097/1‑150 (MQ=255)
                                                                              gccgcGCTGTGCTTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                         <  2:3572900/150‑1 (MQ=255)
                                                                              gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                         >  2:5343342/1‑150 (MQ=255)
                                                                                  cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                     <  2:1128228/150‑1 (MQ=255)
                                                                                   gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                    <  2:4611321/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:4053266/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:513855/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:647821/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:5216754/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:5474699/150‑1 (MQ=255)
                                                                                         cGTTGTTCAGAATCTGCTTGGTCGATACCACGTAAATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  2:8149595/150‑1 (MQ=255)
                                                                                         cGTTGTCCAGCATCTGCTTTGTCGATACCACTTACATTTTCTTATCCTGTCGTTTGTTTTCGATTACAAAATATAATCAAATTTTTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                              <  1:312189/150‑1 (MQ=255)
                                                                                           tttttCAGATTCTGTTTTGTCTATACCACTAACTTTTCAATATCCTGTCGTTTTTTTTCTTTTTAAAATAATATTTAATTTATTTTTTTTTAAATATCTAGATAACTATCAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                            <  1:7188776/147‑1 (MQ=255)
                                                                                           ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAAAGTAAATATTTTTTTTTAAATATAGATAGAACGAACAAAAAAAAAAAAAAAAGAAAATTAATAGAGGAGTGtaatg                                                            >  1:4095782/1‑148 (MQ=255)
                                                                                              ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTAAAAATATTCGAGGAGTGAAAGGCaa                                                         >  2:7935616/1‑150 (MQ=255)
                                                                                               tCAGCATCTGCTTTGTCGATACCACTTACATTTTAATATCCTGTCGTTTTTTTTCTATTAAAAAATATAATCAAATTATTTTTTTTTAAATATCTATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                        <  1:1049947/150‑1 (MQ=255)
                                                                                                   cATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                    <  2:1395592/150‑1 (MQ=255)
                                                                                                   cATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                    <  2:1589801/150‑1 (MQ=255)
                                                                                                    atgtGCTTTGTCTATACCACGTAATTTTTCATAGCCTTTCTTTTGTTTTAGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                   <  1:3980887/147‑1 (MQ=255)
                                                                                                    aTCTGCTTTTTCGATACCACTTAAATTTTCATATCCTTTCTTTTGTTTTCGATTTAAAAATATAATCAAATTATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                   <  1:8209160/150‑1 (MQ=255)
                                                                                                    aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                   <  2:2773145/150‑1 (MQ=255)
                                                                                                     tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAATATAAAGAAAATAATTTTTTTTAAAAATAGAGATAAACAGAACAAAAAAATCAATATTTAAAATATTATAGGAGTGAGAGGGgaaaaaaag                                                  >  1:5085082/1‑148 (MQ=255)
                                                                                                      cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                 <  2:5960261/150‑1 (MQ=255)
                                                                                                              tCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAAATATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                         >  2:2496362/1‑150 (MQ=255)
                                                                                                                gATACCACGTACATTTTCATATCCTGTGGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAGAACGACAATATGAAAATTATTAGAGGAGTGAAATGCAAAAAAACGTCCTCCCGATa                                       >  2:4758736/1‑150 (MQ=255)
                                                                                                                gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAAATATTTGTTTTTTAATATCGAGATAACGATAACAAGAACCACAAAATTAAAAATATTAGAGGGGTGAAAGGCAAAAAAAAGGCCTCCCGATa                                       >  2:893569/1‑150 (MQ=255)
                                                                                                                gATACCAATTAATTTTTAATATCCTTTCTTTTTTTTTCTATTTAAATATATAATTAATTTTTTTGTTTTTAATTTTCTTTTTAACTATCACAAAAACTAAAATATTAAAATTATTCGTGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                       <  1:6142253/150‑1 (MQ=255)
                                                                                                                  tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                     >  2:1994037/1‑150 (MQ=255)
                                                                                                                     cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                  >  2:574913/1‑150 (MQ=255)
                                                                                                                      aCGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAAAGACAAAATGAAAATTATTAGTGGAGGGAAAGGCAAAAAAAACGCCTCCCGATAGGGAAg                                 >  2:1763580/1‑150 (MQ=255)
                                                                                                                         tACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCg                              >  2:3074792/1‑150 (MQ=255)
                                                                                                                           cATTTTCATATCCTGTCTTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAATTATCGTGTTAACGTTCACAAAAACGACAATTTGAAAATTATTCGAGGAGTGTAAGGCATATAAACGGCCTCCCGATAGGGAAGCCGTa                            <  2:3399509/150‑1 (MQ=255)
                                                                                                                               ttCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtttttt                        <  2:1453711/150‑6 (MQ=255)
                                                                                                                               ttCATATCCTGTCGTATGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtttttt                        <  2:6541918/150‑6 (MQ=255)
                                                                                                                                tCATATCGTGTCGTTTGTTTTCTATTTAAAAATATATTTAAATTTTTTGTTTTTAAATTTCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  1:6433166/150‑1 (MQ=255)
                                                                                                                                tCATACCCTGACGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                       <  2:4726060/150‑1 (MQ=255)
                                                                                                                                      ccTGTCTTTTGTTTTCGATTACAAAATATAATGAAATTATTTGATTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                 <  1:679800/150‑1 (MQ=255)
                                                                                                                                        tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt               <  1:4720918/150‑1 (MQ=255)
                                                                                                                                           cttttgtttTCGATTTAAAAATATAATGAAATTTTTTGTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt            <  1:2859035/148‑1 (MQ=255)
                                                                                                                                           agtttgtttTCTTTTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACATAAACGACTATATGAAAATTATTCGATGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt            <  2:3307235/149‑1 (MQ=255)
                                                                                                                                             tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt          >  2:5854051/1‑150 (MQ=255)
                                                                                                                                               tgtttTCGATTTCAAAATATAAGGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAACAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa        <  2:4140570/150‑1 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg   >  2:6715422/1‑150 (MQ=255)
                                                                                                                                                    tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg   >  1:5209151/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGG  >  NC_000913/2177079‑2177377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: