Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 14.2% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC14.2% 445.9 / 11.3 241intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (57/113);  new base C (23/9);  total (92/149)
Fisher's exact test for biased strand distribution p-value = 1.14e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326
                                                                                                                                                        |                                                                                                                                                       
    ggtggtGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑gggg                                                                                                                                                    <  2:5447312/150‑1 (MQ=255)
     gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑g‑gg‑ggggg                                                                                                                                                   >  1:2255806/1‑150 (MQ=255)
      tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑g‑gg‑gggggg                                                                                                                                                  >  1:3662295/1‑149 (MQ=255)
       ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGggcg                                                                                                                                                 <  1:3969903/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGggcgg                                                                                                                                                <  2:4486673/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGggcgg                                                                                                                                                <  1:5499222/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGggcgg                                                                                                                                                <  1:582836/150‑1 (MQ=255)
        gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGggcgg                                                                                                                                                <  1:7787824/150‑1 (MQ=255)
           aCTGGCGTATTTAAGATGATATTTTAAATTTAATTAATGTCATCAGGTCCGAAAATAACGAGTTTATTTCTGTCTGTCATCCTGTTGCGGTCGTGTCATGTGCATTGGTTCA‑TATAATCTCTGGCGCTAGGAGCGC‑G‑CT‑gggggggggga                                                                                                                                             >  2:8185144/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGCGG‑CC‑GGGGGGGGCCac                                                                                                                                            >  2:697187/1‑149 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CG‑GGGGGCGGGCaaa                                                                                                                                           >  1:469616/1‑149 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGcccca                                                                                                                                           >  2:5996749/1‑147 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCaaa                                                                                                                                           >  2:1158571/1‑149 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCaaa                                                                                                                                           >  2:5292434/1‑149 (MQ=255)
             tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TAGAATCACTGGCGCAAGGAGCGC‑G‑GA‑GGGGGGGGcccaa                                                                                                                                           >  1:3313722/1‑147 (MQ=255)
              ggCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGcccaaa                                                                                                                                          >  2:1217713/1‑146 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑GC‑GGGGGCGGCCAAAcg                                                                                                                                         >  1:3942403/1‑150 (MQ=255)
               gCGTATTTAAGATGATATTTTAAAATTAATTAAAGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCCGCCaaaac                                                                                                                                         >  2:1890757/1‑147 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGCGG‑CC‑GGGGGCGccccacccc                                                                                                                                        >  2:726420/1‑144 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑CGGGGGGGcccaaacac                                                                                                                                       >  2:7245775/1‑143 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCATcgcc                                                                                                                                       >  2:6182899/1‑150 (MQ=255)
                 gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATcgcc                                                                                                                                       <  2:5604573/150‑1 (MQ=255)
                  tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCAGTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑GGGGGGGGGCACAcgccg                                                                                                                                      >  2:4992202/1‑150 (MQ=255)
                    tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCATcgccccc                                                                                                                                    >  2:466405/1‑150 (MQ=255)
                     ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATcgccgccg                                                                                                                                   <  1:159995/150‑1 (MQ=255)
                      tAAGATGATCTTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATcgccgccgc                                                                                                                                  <  2:3419346/150‑1 (MQ=255)
                      tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATcgccgccgc                                                                                                                                  <  2:5588260/150‑1 (MQ=255)
                      tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATcgccgccgc                                                                                                                                  <  2:5588246/150‑1 (MQ=255)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGcccc                                                                                                                               <  2:3497965/150‑1 (MQ=255)
                         gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGcccc                                                                                                                               <  2:2763647/150‑1 (MQ=255)
                           tgatCTTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCTAGGAGCGC‑G‑CA‑GGGGGCGGCCTATCGCCGACGCCCCCt                                                                                                                             <  2:8218342/150‑1 (MQ=255)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTGTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGGGGCCCCACGGCGCGGCCCCCTgg                                                                                                                           >  2:459893/1‑149 (MQ=38)
                             atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGGCCAACCCCGCCCCCCCCGGc                                                                                                                           >  1:4598936/1‑150 (MQ=38)
                                ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGCGG‑GC‑GGGGGGGGCCAAACccccccccccccccccc                                                                                                                         >  1:3520508/1‑145 (MQ=255)
                                ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCAAAGCCGCCCCCCCCAGCAcc                                                                                                                        >  1:4848330/1‑150 (MQ=38)
                                   tAAATTTATTTAATGTCATCTGGTCCTAAAATAACTATATTTTTTCTGTCTCTAATCGTGTTGCGCTCCTGTCTTGTGCTTTGATTAA‑TTTAATCAGTGGCGCATGGAGCGC‑G‑CT‑GGGGGGGGCCAATCGCCGCCGCCCCCTGCAccccc                                                                                                                     <  1:4928534/150‑1 (MQ=19)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑GA‑GGGGGCGGGCCAAAGCCCCCCCCCCCCGCccccccccg                                                                                                                  >  2:7999947/1‑147 (MQ=255)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCAACGCCGCCCCCCCCCGCACCCCCcgg                                                                                                                  >  2:6684245/1‑150 (MQ=38)
                                      aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑CGGGGCGGCCCAAAGCCCCCCCCCCCCCCCCCCCCcgg                                                                                                                  >  2:7032748/1‑150 (MQ=38)
                                               atGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑GCCC‑GGGGGCGCCCCAAC‑CCGCCCCCCCCCGCCCCCcccggccccggccc                                                                                                         >  2:6465730/1‑148 (MQ=255)
                                                  tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑CGGGGCGGCCCAAACCCGCCGCCCCCAGCACCCCCGGGCACGGGCGA‑ACa                                                                                                     >  2:1996788/1‑150 (MQ=37)
                                                    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCCGCCCAACGCCGCCCCCCCCCGCCCCCCCCCGCGCAGGCGC‑ACaaa                                                                                                   >  2:3322044/1‑150 (MQ=25)
                                                       cggTCCTAAAAAAACGATAATATTTCTGTCCCACATCGTGTTGCGCTCCGGTCAGGTG‑ATTGGTTTATTATAATCCCGGGCGCACGGCGCGC‑G‑CC‑GGGGGCGGCAAAGCGCCGCCGCCCCCCGCACCCCCGGGCTCTGGCGA‑ACAAAATc                                                                                                <  1:7630662/149‑1 (MQ=12)
                                                       aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑GG‑GGGGGGGGGCCCAAGCCCCCCCCCCCCCCCCCCCCCGGCGCGGGCGC‑ACaaaaaa                                                                                                >  1:4449914/1‑148 (MQ=255)
                                                       aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑GGGGGGGGCCCAAAGCCCCCCCCCCCCGCACCCCCCGGCGCCGGGGA‑ACaaaaaa                                                                                                >  1:7383160/1‑148 (MQ=255)
                                                         gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgc                                                                                              <  2:778945/150‑1 (MQ=255)
                                                         gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgc                                                                                              <  2:7318233/150‑1 (MQ=255)
                                                            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑GG‑GGGGGGGGGCCCACGCCCCCCCCCCCCCCACCCCCCCCCGCCGGCGC‑ACaaaaaaacccc                                                                                           >  1:1645128/1‑143 (MQ=255)
                                                            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑GG‑GGGGGGGGCCCAAAGCCGCCCCCCCCACCACCCCCCGGCGCAGGGGAGACaaaaaacccc                                                                                            >  2:2289947/1‑144 (MQ=255)
                                                             gAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGGCAAAAGCCCCCGCCCCCGCCACCCCCCCGCGCGTGCGG‑ACAAAAAAGCCGCt                                                                                          >  1:8117427/1‑150 (MQ=25)
                                                                      gagaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCCCCGCCGCCCCCAGCACCCCCCGGCACGGGCGT‑ACAAAAACCCCGCGGCGCGGGGc                                                                                 >  2:2782518/1‑150 (MQ=21)
                                                                            ttttCAGTCTCTCATCCTGTTGCTCTCGTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCATGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCTCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑AAAAATTCGCCGCTGCGCGTTGCCCTCgg                                                                           <  2:7732124/149‑1 (MQ=18)
                                                                            ttttCAGTCTCTAATCCTGTTGCGCGCCTGTATTGTGCTTTGCTTCA‑TATAATCCCTGGCGCATGGAGCGC‑G‑CG‑GGGGGGGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  1:1274682/149‑1 (MQ=18)
                                                                            atTTCTGTCTCTAATCGTGTTGCGCTCCTGCCTTGTGCTTTGCTAAA‑TATAAACACGGGCGCATGGCGCGG‑G‑CT‑GGGGGGGGCCACCCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  1:3268037/150‑1 (MQ=17)
                                                                            atTTCCTTCTCCCACCCTGTTGCGCTCCTTTCTTGTGAATTGATAAA‑TATAATCCCGGGCCAATGGTGCGC‑G‑CT‑GGGGGCGGCAACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  1:2726030/150‑1 (MQ=14)
                                                                            atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:4051107/150‑1 (MQ=255)
                                                                            atTTCAGTCTCTCAGCCTGGTGCGCTCCTGTCATGTGCATTGATTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCATTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                           <  2:2255806/150‑1 (MQ=255)
                                                                                 aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                      <  2:6534303/150‑1 (MQ=255)
                                                                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATc                                                                     <  2:5861048/150‑1 (MQ=255)
                                                                                      ctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCCCCCCCCCCCCCAGCACCCCCCCGCGCTGGCGA‑ACAAAATCGCCCCTGCGCGGTGCCCTCGGCGTATcccat                                                                 >  2:7465261/1‑148 (MQ=18)
                                                                                       tcATCCTGTTGCGCTCCTGTCTTGTGCATTGATTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CT‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                <  1:8218553/150‑1 (MQ=255)
                                                                                       ccATCCTGTGGCGCTCCTTCCATGTGCATTGCTTCA‑TATAATCACGGGCGCATGGAGCGC‑G‑CT‑GGGGGCGGCCATGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                <  1:6583834/149‑1 (MQ=17)
                                                                                         aTCGTGTGGCGCTCCTGTCTTGTGCTTTGCTTCA‑TATAATCCCTGGCGCCCGGAGGGC‑G‑CT‑GGGGGCGGCCTATCCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACg                                                              <  1:2376621/150‑1 (MQ=17)
                                                                                         aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGGGGGCCAAAC‑CCCCCCCCCCCACCCCCCCCCCGCCATGGCGA‑AAAAAAAAACCCCGGCGCGGTGCCCACCGCGTAAcccaaaaa                                                              >  2:4203274/1‑145 (MQ=12)
                                                                                          tcgtGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACGGGCGCATGGAGCGC‑G‑CA‑GGGGGCGCCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                             <  1:1784489/147‑1 (MQ=255)
                                                                                          tCCTGTGGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                             <  1:2460790/150‑1 (MQ=255)
                                                                                                 gcgcTCCTGTCATGTGCTTTGCTTCA‑TATAATCCCGGGCGCAAGGAGCGC‑G‑CT‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  1:113177/150‑1 (MQ=255)
                                                                                                 gcgcTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  2:7929740/150‑1 (MQ=255)
                                                                                                 gcgcTCCTGTCAGGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                      <  1:7996512/150‑1 (MQ=255)
                                                                                                   gcTCCTTTCTTGTGATTTGCTTAA‑TATAATCACGGGCGCATGGAGCGC‑G‑CG‑GGGGGCGGCAAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                    <  1:6248295/150‑1 (MQ=14)
                                                                                                    cTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CT‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                   <  1:7086150/150‑1 (MQ=255)
                                                                                                         gTCTTGTTCATTGCTTCA‑TATAATCACTGGCGCATGGAGCGC‑G‑CT‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                              <  1:6626545/150‑1 (MQ=255)
                                                                                                            aTGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCAAACCCCCCCCCCCCCCCACCCCCCGGCGCTGGCCC‑ACAAAAACGCCCCGGCGCCGGGCCCCCCGCGTATCACCTAACGCGACCCCGGCGGgcgccc                                           >  2:5900895/1‑148 (MQ=9)
                                                                                                              gtgCATTGATTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                         <  1:2781706/150‑1 (MQ=255)
                                                                                                               tgCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCCATCGCCGCCCCCCCCGGCACCCCCCGGCTCTGGCGA‑ACAAAAACCCCGCGGCGCGGTGCCCTCGGCGTATCCCTTACGGCTAACCGGTCGGGCGCGCGGt                                        >  2:6091590/1‑150 (MQ=14)
                                                                                                               tgCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                        <  1:5260075/150‑1 (MQ=255)
                                                                                                                 cATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                      <  2:5153548/150‑1 (MQ=255)
                                                                                                                      cTTCA‑TATAATCCCTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                 <  1:6506745/150‑1 (MQ=255)
                                                                                                                      cTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGATTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                 <  2:210702/150‑1 (MQ=255)
                                                                                                                       ttCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGGGGCCAAACGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                <  1:4187492/150‑1 (MQ=255)
                                                                                                                       ttCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                                <  2:3097211/150‑1 (MQ=40)
                                                                                                                        tCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CC‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:1506841/150‑1 (MQ=255)
                                                                                                                        tCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:3640343/150‑1 (MQ=39)
                                                                                                                         tcctataATCACGGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                               <  2:3899180/147‑1 (MQ=255)
                                                                                                                          a‑tatAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                             <  1:597445/150‑1 (MQ=38)
                                                                                                                              taATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAAACGCCGCCGCCCCCAGCCCCCCCGGGCGCTGGCGA‑ACAAAAACGCCGCGGCGCGGTGCCCGCGGCGTATCCCGTACGGCTACCGGGTCGGGCGCGAGGGAACATCCCAGTaaa                          >  2:4779488/1‑150 (MQ=11)
                                                                                                                               actCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaaa                         <  1:5031863/148‑1 (MQ=17)
                                                                                                                                aTCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                        <  2:6762824/150‑1 (MQ=33)
                                                                                                                                  cACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                      <  1:6071507/150‑1 (MQ=32)
                                                                                                                                   aCTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                     <  1:4497685/150‑1 (MQ=32)
                                                                                                                                    cTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                    <  1:4348940/150‑1 (MQ=32)
                                                                                                                                         gcAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATAGCCCCCGCCCCCTGCACCCCCCGGCTCTGGCGA‑ACAAAAACCCCGCGGCGCGGTGCCCTCGGCTTATCCCATAAGGCTACCGGGTCGGGCGCGAGGGAACAACCCAGTAAAAAGCGCCcaca               >  2:1273815/1‑150 (MQ=11)
                                                                                                                                          cAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg              <  2:1114193/150‑1 (MQ=32)
                                                                                                                                            aGGAGCGC‑G‑CA‑GGGGGCGCCCAATCGCCGCCCCCCCCTCCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  1:1423249/150‑1 (MQ=12)
                                                                                                                                            aGGAGCGC‑G‑CA‑GGGGGCGACCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc            <  2:1723126/150‑1 (MQ=17)
                                                                                                                                                gcgc‑g‑cA‑GGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca        <  2:6173579/150‑1 (MQ=17)
                                                                                                                                                gcgc‑g‑cA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca        <  2:3130142/150‑1 (MQ=32)
                                                                                                                                                  gc‑g‑cA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAGCCCTGTAAAACGCGCCCTCAGCCCACATc      >  2:2375433/1‑150 (MQ=17)
                                                                                                                                                   c‑g‑cA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAtgg     <  1:4058157/150‑3 (MQ=17)
                                                                                                                                                   c‑g‑cA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc     <  2:3844298/150‑1 (MQ=32)
                                                                                                                                                        |                                                                                                                                                       
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCA‑TATAATCACTGGCGCAAGGAGCGC‑G‑CA‑GGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG  >  NC_000913/2229028‑2229326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑