Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,436 C→A 11.8% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4360CA11.8% 985.4 / 31.3 326Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (102/176);  new base A (45/3);  total (147/179)
Fisher's exact test for biased strand distribution p-value = 2.78e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585
                                                                                                                                                    |                                                                                                                                                     
tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                      <  1:1555415/150‑1 (MQ=255)
tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCa                                                                                                                                                      >  2:6007132/1‑150 (MQ=255)
tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCa                                                                                                                                                      >  1:1883045/1‑150 (MQ=255)
   ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  1:1972498/150‑1 (MQ=255)
   ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  1:4258342/150‑1 (MQ=255)
   ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  1:7392869/150‑1 (MQ=255)
   ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                   <  2:1830851/150‑1 (MQ=255)
     ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACa                                                                                                                                                 >  2:5410813/1‑150 (MQ=255)
        taaTATCTCATTTTTATTTGTAATTTTTCGGGGAAGTATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                              <  1:6365169/149‑1 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAATa                                                                                                                                              >  2:162360/1‑150 (MQ=255)
        gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATCAAGaaaaaa                                                                                                                                              >  1:334425/1‑148 (MQ=255)
           tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                           <  1:1808739/150‑1 (MQ=255)
            atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                          <  1:4635681/150‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAGGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  2:769851/150‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  1:1454566/150‑1 (MQ=255)
             tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                         <  1:2738135/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAAAATCGATGt                                                                                                                                       >  2:7078062/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                       <  2:127198/150‑1 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                                                                                                       >  2:5435656/1‑150 (MQ=255)
               tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGt                                                                                                                                       >  1:686061/1‑150 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                   <  1:4481032/150‑1 (MQ=255)
                    tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                                  <  2:7310107/150‑1 (MQ=255)
                         gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                             <  2:6855373/150‑1 (MQ=255)
                         gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                             <  1:5298082/150‑1 (MQ=255)
                          tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                            <  2:2324037/150‑1 (MQ=255)
                              aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAATAAAGAAAAAAGAGAAGTTAAACAAAAAGTTTa                                                                                                                        >  1:2271532/1‑150 (MQ=255)
                                ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:406431/150‑1 (MQ=255)
                                ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:7722326/150‑1 (MQ=255)
                                ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                      <  1:5992977/150‑1 (MQ=255)
                                  ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTTTTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                    <  1:5747215/150‑1 (MQ=255)
                                      gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                                <  2:1105242/150‑1 (MQ=255)
                                      gggAAGGATATAGAGATTAAATCAAGCATTTATTTGGAATGTATATTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                                <  2:6392106/150‑1 (MQ=255)
                                        gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                              <  1:1079428/150‑1 (MQ=255)
                                           ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCaa                                                                                                           >  2:7380855/1‑150 (MQ=255)
                                           ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGAAAAAAGCGATGTAAAGCAATAAGTTTAAATTTCGCGCCaa                                                                                                           >  2:3349805/1‑150 (MQ=255)
                                              tataGATATTTAAGCAATCATTTATTTGGAATTTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                        <  2:5071084/150‑1 (MQ=255)
                                              tataGAGAATTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                        <  2:3885027/150‑1 (MQ=255)
                                               ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAAGAAAAAAACAAAAAAGCGAATTAAAGCCCCAAGATTAAATTGCCCGccccacca                                                                                                       >  1:1261641/1‑144 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGAGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                    >  2:6664023/1‑150 (MQ=255)
                                                    gaTTTACGCAAGCATTTCTTTGGAATGTATTTTTCGTTCGACCTGTGCTTATACATCACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                  <  2:26780/150‑1 (MQ=255)
                                                     aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAATACAAAATCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCATTgcgcg                                                                                                 >  2:1973163/1‑150 (MQ=255)
                                                          agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                            <  2:4473799/150‑1 (MQ=255)
                                                            caagcaTTTATTTGGAATTGATTTTCCGTTCGACCTTTGCTTACACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTCCGCGCCAATCATGGCGCGCACAAGc                                                                                          <  2:4215345/150‑1 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAATCGGTGTAAATCAAAAATTTTAAATTTAGCGCCCGGCAAGGCGCGCACaaaca                                                                                         >  1:678947/1‑147 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATAAAATATAGATGTAAAACACTAAGTTTAAATTTCGCGACAATCATGGCGCGCAAAACCt                                                                                         >  2:8249089/1‑150 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATAAAAGAAAAAAAAAAAAAAAAGATGTAAAGCACGAAGTTTAAATTTAGCGCCCCTCCAGGCGCGcaccacct                                                                                         >  1:7775076/1‑145 (MQ=255)
                                                              cgcaTTTATTTGGAATGTATTTTTCGTTCGACCTGTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                        <  2:3267979/149‑1 (MQ=255)
                                                              agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTGGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                        <  2:6298942/150‑1 (MQ=255)
                                                                caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGACATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtata                                                                                      <  2:3188015/150‑1 (MQ=255)
                                                                   ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                                   <  2:8075513/150‑1 (MQ=255)
                                                                    tatttGGAATGTATTTTTCTTTCTACTTTTGCTTATACATAACAGTTTTTTTTTTATTTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAACGCACTAAGTTTAAATTGCGCGCCATTCATGGCGCGCACAAGCTATAATAc                                                                                  <  1:6577470/150‑1 (MQ=255)
                                                                       ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGAGCCAATCATTGAGCGCACAACCTATAATACCaa                                                                               >  2:4715877/1‑150 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                         <  1:4309295/150‑1 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                         <  2:2074234/150‑1 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAAACAAAATAGATGTAAATCACTAAGTTTAAATTGAGCGCCAAACATGGCGCGCACAATCTATAAAACCAACCTAAt                                                                         >  2:64731/1‑150 (MQ=255)
                                                                                  ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAATAAAATAGCGATGTAAATCACTAAGTTTTTATTTCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCTAATTtctc                                                                    >  2:3453005/1‑150 (MQ=255)
                                                                                  aatacgtacgACCATTGATTATAAAAAACACACCATTTTATAATATATAAAATAATACAAAGAAATCAAGACAAAACCCATCAAAACCACTAACTTTAAATTGCCCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtcac                                                                    <  2:2945306/146‑3 (MQ=255)
                                                                                     tcgttcgACCTTTGATTATACATAACTGTCTTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                                 <  1:8052165/150‑1 (MQ=255)
                                                                                      cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                                <  2:4879726/150‑1 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAAGTTTAAAATGCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAACCAAAATTctcctcctc                                                              >  2:4003154/1‑150 (MQ=255)
                                                                                        ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAAACAAACAAAACAACACAAAAGCCATTTAAAGCACTAAGTTTAAATTTCGCGCCAAACAATGCGCCCCCAAGCAATAATACCAACCAAAATTcaccaccac                                                              >  2:5683890/1‑148 (MQ=255)
                                                                                         tcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAATACAATATCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCTATAAAAACAACCTAATTTCTCCTCcact                                                             >  2:5187482/1‑150 (MQ=255)
                                                                                           gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                           <  2:4568732/150‑1 (MQ=255)
                                                                                             ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                         <  2:3519234/150‑1 (MQ=255)
                                                                                              cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAATAGCGATGTAAAGCACTAAGTTTAAAATTCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACCTAAATTAAACTCCTCTTagag                                                        >  2:1372665/1‑150 (MQ=255)
                                                                                                 tGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  1:2746065/150‑1 (MQ=255)
                                                                                                 tGCTTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                     <  1:545498/150‑1 (MQ=255)
                                                                                                  gCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAATAAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTTAGCGCCAAAAATTGAGCGCACAAGCAATAAAACAAACCAAATTTAACCTACACTTAGAGTGAc                                                    >  2:3127967/1‑150 (MQ=255)
                                                                                                   cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                   <  1:1192233/150‑1 (MQ=255)
                                                                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATAACAAACTAATTTCTCCTCCTCTTAGAGTTACta                                                  >  2:614737/1‑150 (MQ=255)
                                                                                                       taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                               <  1:5415031/150‑1 (MQ=255)
                                                                                                         cATAACAGTCGTTTTTTTTATTTATAAAAAAAATAAAAGAAATAAATACAAAATCGATGTAAAGCACTAAGTTTAAAATTATCGCCCATCATTGCGCGCCCAAGCAATAATAACAACCGAAATTCTCCTCCGATTAGAGTGACTatatat                                             >  2:5624542/1‑148 (MQ=255)
                                                                                                                gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGTTGTAAAGCACTAAGTTTAAAGTGCGCGCCAATCATGGCGCGCACAAGCTATAAGACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGAtc                                      <  1:1375246/150‑2 (MQ=255)
                                                                                                                gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                      <  2:2440583/150‑1 (MQ=255)
                                                                                                                  cGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                    <  1:6476068/150‑1 (MQ=255)
                                                                                                                   gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                   <  2:7287043/150‑1 (MQ=255)
                                                                                                                    tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                  >  1:882133/1‑150 (MQ=255)
                                                                                                                      tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                <  2:7772585/150‑1 (MQ=255)
                                                                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                              <  1:2585832/150‑1 (MQ=255)
                                                                                                                            aTTTATAATATATTTCAAAGAACTCAAGACTATAGCTATTTAAAGCACTAAGTTTAAATTGCGCGCCATTCATGTCGAGCACATGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          <  1:6884686/150‑1 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                          <  1:365344/150‑1 (MQ=255)
                                                                                                                            aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGAAATGCACTAAGTTGAAATTTCGCGCCAGTCGTGGCGCGCACAAGCTATAATACAAACATAATTTCTCCTCCTCTTAGAGTGACTATATCTCCAGATAGAATTCAGGTAt                          >  1:4784927/1‑150 (MQ=255)
                                                                                                                               tagaaaaTAATTCAAAGAACTCAAGACAATAGCGAGGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                       <  2:7903634/147‑1 (MQ=255)
                                                                                                                                ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      <  1:7956598/150‑1 (MQ=255)
                                                                                                                                ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                      >  1:1646051/1‑150 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     >  1:331134/1‑150 (MQ=255)
                                                                                                                                 taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                     >  2:4413590/1‑150 (MQ=255)
                                                                                                                                   aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                   >  1:5638476/1‑150 (MQ=255)
                                                                                                                                       aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa               <  1:5348719/150‑1 (MQ=255)
                                                                                                                                       aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa               <  2:4889094/150‑1 (MQ=255)
                                                                                                                                         ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg             >  1:7190548/1‑150 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  2:7509675/150‑1 (MQ=255)
                                                                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt         <  2:5310522/150‑1 (MQ=255)
                                                                                                                                               gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa       >  2:5127392/1‑150 (MQ=255)
                                                                                                                                                aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAACTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCACCCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt      <  2:734583/150‑1 (MQ=255)
                                                                                                                                                aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt      <  2:678947/150‑1 (MQ=255)
                                                                                                                                                aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt      <  2:5925637/150‑1 (MQ=255)
                                                                                                                                                  aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc    >  1:3827220/1‑150 (MQ=255)
                                                                                                                                                  aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc    >  1:5182939/1‑150 (MQ=255)
                                                                                                                                                    cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt  <  2:2461434/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGT  >  NC_000913/2470288‑2470585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: