Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,662,524 C→G 5.9% intergenic (‑393/+59) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,662,5240CG 5.9% 1488.3 / 15.3 456intergenic (‑393/+59)iscR/trmJisc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (300/121);  new base G (3/32);  total (303/153)
Fisher's exact test for biased strand distribution p-value = 1.53e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 463 total aligned reads displayed.

TTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGG  >  NC_000913/2662375‑2662673
                                                                                                                                                     |                                                                                                                                                     
ttACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAc                                                                                                                                                       >  1:977298/1‑150 (MQ=255)
 tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGTGAACGCCTTATCCGACCTACg                                                                                                                                                      >  1:4075705/1‑150 (MQ=255)
     cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAGGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTc                                                                                                                                                  <  2:166831/150‑1 (MQ=255)
     cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTc                                                                                                                                                  >  2:4468443/1‑150 (MQ=255)
      gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCt                                                                                                                                                 <  2:6439438/150‑1 (MQ=255)
           aTCTGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAGGGTTCTGTTCACTGTAGGCCTGATACGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGTGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCa                                                                                                                                            <  2:3211706/150‑1 (MQ=255)
           aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCa                                                                                                                                            <  2:110198/150‑1 (MQ=255)
            tatggaaaCGGGTGTCGGATGCGGCGTTAACGCCTTATCCGACCTTCGGTTCTGTTCACTGTAGGCCTGATAAGACGAATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAc                                                                                                                                           <  2:7791068/144‑1 (MQ=14)
            tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAc                                                                                                                                           <  2:4744047/150‑1 (MQ=255)
              tgcaaaCGGCTGTCGGTTGCGGCGTGAAGGCCTTAACCGCCCTCCGGTTGTGTTCACTGTAGGCCTGATATGACGAATTACGGGTCGCATATGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTg                                                                                                                                         <  2:3791886/146‑1 (MQ=14)
               ggCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCCACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGt                                                                                                                                        >  1:812128/1‑150 (MQ=255)
                gCAACGGGTGTCGGTTGCGGCGTGAACGCCTTATCCGCCCTACGTTTCTGTTCACTGTAGGCCTGTTAAGACGCATTACGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTa                                                                                                                                       <  1:1184486/150‑1 (MQ=14)
                gCAACGGCTGTCGGATGCGGCGTGAACGCCGTATCCGACCTAAGGTTCTGTTCACTGTATGCCTGATAAGACGCATTAAGCGTCGCCTCAGGCCACGGCTGTCGGATGCGGCGGGACCGCCTTATCCGACCTACGGTTCTGTGAACTGtt                                                                                                                                       >  1:3779644/1‑149 (MQ=14)
                 aaaCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACATACGGTTCTGTACACTGTAGGCCTGATAAGACGAATTACGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAg                                                                                                                                      <  1:5437007/149‑1 (MQ=255)
                  aaCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGGGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAgg                                                                                                                                     >  1:4329593/1‑150 (MQ=255)
                   aCGGCTGTCGGATGCGGCGTGAACGCATTATCCGACATCCGGTTCTGTTCACGGTAGGCCTGATCAGACGCAATACGCGTCGCACCAGGCCACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGc                                                                                                                                    <  1:1916948/150‑1 (MQ=14)
                     ggCTGTCGGTTGCGGCGTGAACGCATTATCCGACCTAGGGTTCTTTTCACTGTTGGCCTTATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCt                                                                                                                                  <  1:3270263/150‑1 (MQ=14)
                      gCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATAACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTg                                                                                                                                 <  2:2438147/150‑1 (MQ=255)
                           cGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAg                                                                                                                            <  2:8067300/150‑1 (MQ=255)
                            ggctGCGGCGTGAACGCCTGATCCGACCTCCGGTGCTGTACACTGTAGGCCTGATAAGACGCTTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGa                                                                                                                           <  2:4254155/147‑1 (MQ=11)
                            ggATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGa                                                                                                                           >  1:2189015/1‑150 (MQ=255)
                             gttGCGGCGTTAACCCCTTTACCTACCTTCGGTTCTCTTCACTTAAGTCCTCATATCAAGCATTACGCGTCGCATCTGGAAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGAc                                                                                                                          <  1:6233488/148‑1 (MQ=14)
                                  ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                                                                                     >  2:2091114/1‑150 (MQ=255)
                                   gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTa                                                                                                                    >  2:6619727/1‑150 (MQ=255)
                                   gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGTACGCCTTATCCGACGTACGGTTCTGTTCTGTGTAGGGCTGATTAGAGGCATTa                                                                                                                    >  1:1474116/1‑150 (MQ=14)
                                    cgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAc                                                                                                                   >  1:7036260/1‑150 (MQ=255)
                                    cgTGAACCCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAc                                                                                                                   >  2:6266552/1‑150 (MQ=255)
                                      tGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgc                                                                                                                 >  1:7319400/1‑150 (MQ=255)
                                          cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCg                                                                                                             <  2:906954/150‑1 (MQ=255)
                                          cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTAATCAGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCg                                                                                                             >  2:3399467/1‑150 (MQ=255)
                                          cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGAAGCGCCGTGAACGCCTAATCCGACCTACGGTTCTGTGCACTCTAGGCCTGATAAGACGCAATACGCGTCg                                                                                                             >  2:7550196/1‑150 (MQ=14)
                                          cGCATTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGATTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCg                                                                                                             <  1:590376/150‑1 (MQ=255)
                                             cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                                                                                          >  1:4007454/1‑150 (MQ=40)
                                                aTCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                                       >  1:8191748/1‑150 (MQ=39)
                                                    gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATGACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCATTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCaa                                                                                                   >  1:2247929/1‑150 (MQ=255)
                                                      ccTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACg                                                                                                 >  1:3585786/1‑150 (MQ=35)
                                                          cGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTg                                                                                             >  2:1305299/1‑150 (MQ=32)
                                                             ttCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTa                                                                                          >  1:5263091/1‑150 (MQ=33)
                                                             ttCTGTCCACGGTCGGCCTGATAAGACGCATTCCGCGTCGAATCTGGCACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTCCGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTa                                                                                          <  2:7304418/150‑1 (MQ=11)
                                                                tGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTc                                                                                       >  2:5505250/1‑150 (MQ=35)
                                                                ggtacACTTTGGGCCTTATACGACGCTTTTCGCGTCGCACCCGGCAACGGGTGTGGGATGCGGCGTGAACGCCTTATCCGACCTAGGGTTCTGTTCACTGTAGGCCTTATAAGACGCTTTACGCGTCGCATCAGGCAACGGCTGTTATTc                                                                                       <  2:1504395/146‑1 (MQ=11)
                                                                  ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCgg                                                                                     >  1:5072657/1‑150 (MQ=37)
                                                                    cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                                                                   >  2:5144388/1‑150 (MQ=37)
                                                                     aCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCt                                                                                  >  2:2691975/1‑150 (MQ=38)
                                                                       tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                >  1:4952926/1‑150 (MQ=39)
                                                                       tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                >  1:1293845/1‑150 (MQ=39)
                                                                       tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                >  1:2422842/1‑150 (MQ=39)
                                                                       tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                                                                >  1:7352048/1‑150 (MQ=39)
                                                                       tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTGCCCTGTAGGCCTGATAAGACGCATTACGCGGCGCATCAGGCCACGGCTGTTATTCGGCCTTg                                                                                >  2:2490303/1‑150 (MQ=255)
                                                                              cTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCtt                                                                         >  1:1697501/1‑150 (MQ=255)
                                                                               tGATACGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGCTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                                                                        <  2:1812977/150‑1 (MQ=255)
                                                                               tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                                                                        >  1:392824/1‑150 (MQ=255)
                                                                               tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                                                                        >  1:2574040/1‑150 (MQ=255)
                                                                                    aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGtt                                                                              >  1:4621845/1‑139 (MQ=255)
                                                                                    aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGtt                                                                              <  2:4621845/139‑1 (MQ=255)
                                                                                    aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTct                                                                   >  1:6630415/1‑150 (MQ=255)
                                                                                      aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgc                                                                 >  1:7956680/1‑150 (MQ=255)
                                                                                      aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCGTGTGACCTTTATTctgc                                                                 >  2:3630755/1‑150 (MQ=255)
                                                                                       cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgct                                                                >  2:2204356/1‑150 (MQ=255)
                                                                                        gCATTACGCGTGCCTTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACATTTATTctgctg                                                               <  2:5812308/150‑1 (MQ=255)
                                                                                           tttggcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACGTAGGGTTGTGTTCACTGTTGGCCTGATAAGTCGCATTACGCGTCGCTTCTGGCAACGGGTGTTATTCGGCGTTGTTTCCTTTTTTCTGCTGCTc                                                            >  2:5110900/5‑150 (MQ=17)
                                                                                            tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCa                                                           >  1:4837107/1‑150 (MQ=255)
                                                                                                cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAg                                                       >  1:723376/1‑150 (MQ=255)
                                                                                                cgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAg                                                       >  1:7127637/1‑150 (MQ=255)
                                                                                                 gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGa                                                      >  1:211453/1‑150 (MQ=255)
                                                                                                  tctcATCTGGAACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAGTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                     <  2:7102479/147‑1 (MQ=18)
                                                                                                  tCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                     >  2:2587019/1‑150 (MQ=255)
                                                                                                  tCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa                                                     >  1:6803874/1‑150 (MQ=255)
                                                                                                     cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc                                                  >  1:5375585/1‑150 (MQ=255)
                                                                                                     cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCAGTACGCGTCGCATCAGGCAACGGCGGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc                                                  >  2:6054661/1‑150 (MQ=255)
                                                                                                      aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa                                                 >  2:2749332/1‑150 (MQ=255)
                                                                                                       tttggCACCGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTTGGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg                                                <  2:371486/147‑1 (MQ=255)
                                                                                                       tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg                                                >  2:744168/1‑150 (MQ=255)
                                                                                                       tCAGGCAACGCCTGTCGGATGCGGCGAGAACGCCTTATCCGACCTACGGTTCTGTTCACAGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTAGTTACCTTTATTCTGCTGCTCAATAGAAGACAg                                                >  2:4868343/1‑150 (MQ=18)
                                                                                                           gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTAATTAGCCCATGTTACCCCTATTCAGATCCACAATAGAAGACAGAATc                                            >  2:2856261/1‑150 (MQ=14)
                                                                                                              aggggtGTCGGTTGCGGCTTTAACGCTTTATCCGACCATGGGTTCTTTTATCTGTCGGCCTTATAAGACGAATTACGCGGCGCACCTGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcg                                         <  1:6901435/145‑1 (MQ=255)
                                                                                                               cGGCTGTCGGTGGGGGGGTGAACGCGTTATCCGACCTCGGGTTCTGTTCCCTTTAGGCCTTATACGACGCATTACGCGTCGCATCCGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCcgc                                        <  1:3012438/150‑1 (MQ=255)
                                                                                                                    gTCGGATAAGGCGTTCACGCCGCATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAgg                                   >  2:188999/1‑150 (MQ=25)
                                                                                                                     tCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGa                                  >  1:5538691/1‑150 (MQ=255)
                                                                                                                      cGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGat                                 <  2:5447588/150‑1 (MQ=255)
                                                                                                                        gATGCGGGTTGAACGCCTTATCCGACCTAGGGTTCTGTTCACTGTAGGCCTGATAAGACGCTTTACGCGTCGCATCTGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGatat                               <  1:6065106/150‑1 (MQ=25)
                                                                                                                            cggctTGAACGCCTTATCCTACCAACTGTTCTGTCCATTTTAGGCCTTATAAGACGCATTCCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCaa                           <  1:2836684/150‑1 (MQ=25)
                                                                                                                             ggggTGAACGCCTTATGCGCCCTCGGGTTGTTTGCTGGGTTGGCCTTTTATGACGCATTCGGCTTCGCTTCTGTCCACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAAc                          <  1:3630755/147‑1 (MQ=255)
                                                                                                                             ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAAc                          >  2:7848994/1‑150 (MQ=255)
                                                                                                                                 tGAACGCCTTATCCGACCTTCGGTTCTGTTCACTGTAGGCCTGATAAGACGATTTACGCTTGGCATCTGGAAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt                      <  1:1014500/150‑1 (MQ=37)
                                                                                                                                 tGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTCGGCCTGATACGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt                      <  2:7003100/150‑1 (MQ=255)
                                                                                                                                 tGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCt                      >  1:1775102/1‑150 (MQ=255)
                                                                                                                                   aaCGCCTTATCCTACCTACGGTTCTGTTCAATGTAGGCCTGATAAGACGCATTACTCGTCGCATCAGGCAACGGCTGTAATTCGGCCTTGTTAACTTTATTATTCTTCTCAATAGAAGACAGAATCCCGCGCAGGATATACAAATCCTgg                    >  1:6308238/1‑150 (MQ=21)
                                                                                                                                   aaCGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTgg                    >  1:4266157/1‑150 (MQ=255)
                                                                                                                                      gCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCtt                 >  1:3110995/1‑150 (MQ=255)
                                                                                                                                       ccTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCttt                >  1:3470440/1‑150 (MQ=255)
                                                                                                                                        cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTc               >  2:6015501/1‑150 (MQ=255)
                                                                                                                                         ttctCCGACCTCCGGTTCTGTTCACTGTCGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGGTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGCAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc              <  2:4139498/147‑1 (MQ=37)
                                                                                                                                         ttATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTcc              >  1:5671943/1‑150 (MQ=255)
                                                                                                                                          tATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAGGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCg             <  2:1256848/150‑1 (MQ=255)
                                                                                                                                           aTCCGACATACGTTTCTGTACACTGTAGGCCTGATAATACGAATTACCCGTCGCATCAGCCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCgg            <  1:5110900/150‑1 (MQ=38)
                                                                                                                                             ccGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgc          >  1:7608879/1‑150 (MQ=255)
                                                                                                                                             ccGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGgc          >  1:6159688/1‑150 (MQ=255)
                                                                                                                                                   tACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCAc    <  1:6431549/150‑1 (MQ=255)
                                                                                                                                                    ccGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTCCGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACg   <  1:2682454/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATCCCGCGCAGGATATTCAACTCCTGGCTTTCCGGGCGCGCACGG  >  NC_000913/2662375‑2662673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: