Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,714,133 A→C 7.4% K413T (AAG→ACG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,714,1330AC 7.4% 1531.4 / 21.0 489K413T (AAG→ACG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (287/154);  new base C (2/45);  total (289/200)
Fisher's exact test for biased strand distribution p-value = 5.11e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 527 total aligned reads displayed.

AGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  NC_000913/2713984‑2714282
                                                                                                                                                     |                                                                                                                                                     
  gCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGTTGAGTTACGCCCGAAAACGCGTGCGCAAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGATAAAGAGAAAGAGAAGc                                                                                                                                                     <  1:3046439/150‑1 (MQ=255)
  gCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGc                                                                                                                                                     >  2:2853417/1‑150 (MQ=255)
  gCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGc                                                                                                                                                     >  2:3857032/1‑150 (MQ=255)
   ccGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGcc                                                                                                                                                    >  2:1249048/1‑150 (MQ=255)
    cGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCcg                                                                                                                                                   >  2:2013171/1‑150 (MQ=255)
       tataTTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCgg                                                                                                                                                <  2:6116079/150‑1 (MQ=255)
              aaGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGaaaa                                                                                                                                         >  2:8234049/1‑150 (MQ=255)
                  gCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACg                                                                                                                                     >  2:7042698/1‑150 (MQ=255)
                   ccAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGc                                                                                                                                    >  1:5287157/1‑150 (MQ=255)
                   ccAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGc                                                                                                                                    <  2:2151946/150‑1 (MQ=255)
                       ttAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATc                                                                                                                                >  2:2245015/1‑150 (MQ=255)
                         aaaGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATcgc                                                                                                                              <  2:5273029/150‑1 (MQ=255)
                           aGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGa                                                                                                                            <  2:6871653/150‑1 (MQ=255)
                            gCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGac                                                                                                                           >  1:8016086/1‑150 (MQ=255)
                             cTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGaca                                                                                                                          >  1:7407669/1‑150 (MQ=255)
                                  gTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaa                                                                                                                     >  1:5185596/1‑150 (MQ=255)
                                    tATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCaaaaa                                                                                                                   >  2:6405840/1‑150 (MQ=255)
                                        gatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  2:5942823/150‑1 (MQ=255)
                                        gatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               >  2:3620846/1‑150 (MQ=255)
                                        gatgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATAtt                                                                                                               <  2:7304945/150‑1 (MQ=255)
                                         atgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTg                                                                                                              <  2:5455390/150‑1 (MQ=255)
                                          tgaGTTACGCCCGAAAACGCGTGCGCCAAGCGAAATGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAATAGCTAAAGAGAAAGAGAAGCCGCGGGTGTAATATCGCCATCGCGACACCAAAAATATTgg                                                                                                             >  2:8103737/1‑150 (MQ=255)
                                              ttACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCTACACCAAAAATATTGGTAAg                                                                                                         >  1:2464530/1‑150 (MQ=255)
                                              ttACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAg                                                                                                         >  1:4222309/1‑150 (MQ=255)
                                              ttACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAg                                                                                                         >  1:3372735/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGt                                                                                                   >  2:1479054/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGt                                                                                                   >  2:500774/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGt                                                                                                   >  2:1835438/1‑150 (MQ=255)
                                                    ccGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGt                                                                                                   >  1:4712739/1‑150 (MQ=255)
                                                     cGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTa                                                                                                  <  2:4299793/150‑1 (MQ=255)
                                                     cGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTa                                                                                                  >  2:5651282/1‑150 (MQ=255)
                                                       aacaCGCGTGCGCCAAGCGAAAAGCAGACGGGCAAGCCAGCGATGAAAGTTCTGGCTAAAGGTGCTGAGAAGAAAAAAGCTAAAGCGACCGCGACGCCGCGGGGGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTaaa                                                                                                <  1:4340923/147‑1 (MQ=255)
                                                        aaaCGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAAc                                                                                               <  2:6712897/150‑1 (MQ=255)
                                                         aaCGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAAcc                                                                                              >  1:2775608/1‑150 (MQ=255)
                                                           cgcgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCaa                                                                                            <  2:2583248/150‑1 (MQ=255)
                                                             cgTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGTCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGc                                                                                          <  1:4555572/150‑1 (MQ=255)
                                                                gcgcCAAGCGAAAAGCAGACCGGCAAGCAATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGa                                                                                       <  2:2523833/150‑1 (MQ=255)
                                                                  gcCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAAc                                                                                     >  1:1609756/1‑150 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   >  1:4883886/1‑150 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   <  2:5822012/150‑1 (MQ=255)
                                                                    cAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACgg                                                                                   >  1:4144753/1‑150 (MQ=255)
                                                                       gCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCg                                                                                >  2:3684565/1‑150 (MQ=255)
                                                                        ctaaaaGCAGACCGGCAAGCCATCTAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGt                                                                               <  1:7770050/148‑1 (MQ=255)
                                                                         gAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTg                                                                              >  2:4566893/1‑150 (MQ=255)
                                                                          aaaaGCAGACCGGCAAGCAATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAACGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGc                                                                             <  1:6122474/150‑1 (MQ=255)
                                                                            aaGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCa                                                                           >  1:2040691/1‑150 (MQ=255)
                                                                                 gACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCa                                                                      >  2:131376/1‑150 (MQ=255)
                                                                                 gACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCa                                                                      >  2:2314483/1‑150 (MQ=255)
                                                                                   ccGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCaaa                                                                    <  2:2902753/150‑1 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACg                                                                  >  2:5309292/1‑150 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACg                                                                  >  1:2610616/1‑150 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACg                                                                  >  1:8123528/1‑150 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACg                                                                  >  1:1741972/1‑150 (MQ=255)
                                                                                     ggCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACg                                                                  >  1:6435674/1‑150 (MQ=255)
                                                                                      gCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGa                                                                 >  1:6531809/1‑150 (MQ=255)
                                                                                      gCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGa                                                                 >  2:8176135/1‑150 (MQ=255)
                                                                                          gCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACaga                                                             >  2:5583021/1‑150 (MQ=255)
                                                                                           ccATCGAATAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAACACAAACAGAAGCCGCGGCTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaa                                                            <  2:3167123/150‑1 (MQ=255)
                                                                                           ccATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCACGCGGAACGGGCGTGCCACCGCAAACGACagaa                                                            >  2:7854384/1‑150 (MQ=255)
                                                                                             aTCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACagaaga                                                          >  2:6239254/1‑150 (MQ=255)
                                                                                              tCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAagag                                                         >  2:5105689/1‑150 (MQ=255)
                                                                                                    aaaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtct                                                   >  2:7488771/1‑150 (MQ=255)
                                                                                                     aaGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAAtctc                                                  >  2:6582965/1‑150 (MQ=255)
                                                                                                      aGTTCTGGCTAAACGTGCTGAGAAGAAAAAAGCTAACGAGAACGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCACGCGGAACGGGCGTGCCACCGAAAACGACAGAAGAGTAATCTCa                                                 <  1:1380701/150‑1 (MQ=255)
                                                                                                       ttagtGGATAATCGTGCTGATAAGAAAAAAGCAAAAGAGAAAGAGACGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCTTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCaa                                                <  1:3910331/146‑1 (MQ=255)
                                                                                                       gTACTGGCTAAAGGTGGTGAGAAGAAAAAAGCTAAAGCGAATGAGAAGCCGGGGGTGAAAAAACGCCATCGCGACACCAAAAATTTTGGTAAGCGCCGTAAACCACGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCaa                                                <  1:6753815/150‑1 (MQ=255)
                                                                                                          cTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGc                                             >  1:7608387/1‑150 (MQ=255)
                                                                                                           tGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGcc                                            >  1:460480/1‑150 (MQ=255)
                                                                                                            ggatAAACGTGCTGATAATAAAAAAGATAAAGACAACGCCACGCCGCGGGTGAAAAAACGCCATCGCGACACAAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCa                                           <  1:5540842/147‑1 (MQ=255)
                                                                                                              cTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAgg                                         >  1:2156307/1‑150 (MQ=255)
                                                                                                               tAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGt                                        >  1:350698/1‑150 (MQ=255)
                                                                                                               tAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGt                                        >  1:6965755/1‑150 (MQ=255)
                                                                                                                     tGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGc                                  >  1:921092/1‑150 (MQ=255)
                                                                                                                       cTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCt                                >  2:4821902/1‑150 (MQ=255)
                                                                                                                        tGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTg                               >  1:1114824/1‑150 (MQ=255)
                                                                                                                         gagaAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTgg                              >  1:7146717/1‑150 (MQ=255)
                                                                                                                          agaagaAAAAAGCTAAAGAGAACGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGt                             <  2:1056554/150‑1 (MQ=255)
                                                                                                                            aagaaAAAAGCTAAAGAGAAAGAGACGCCGCGGGGGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTAt                           <  1:95700/150‑1 (MQ=255)
                                                                                                                            aagaaAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTAt                           <  2:4387167/150‑1 (MQ=255)
                                                                                                                               aaaaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                        >  2:4716531/1‑150 (MQ=255)
                                                                                                                               aaaaaaGCTAAAGAGAAAGAGAAGCCACGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaa                        >  2:277327/1‑150 (MQ=255)
                                                                                                                                 aaaaGCTAAATCGAAAGAGACTCCGCTGGTTAAAAAACGCCATCGCTACACCAAAAATATTGTTAAGCGCCGTAAACCAAGCGTAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      <  1:708010/150‑1 (MQ=255)
                                                                                                                                 aaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      <  2:1708246/150‑1 (MQ=255)
                                                                                                                                 aaaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTaaaa                      <  2:6185097/150‑1 (MQ=255)
                                                                                                                                  aaaGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAg                     >  1:3267767/1‑150 (MQ=255)
                                                                                                                                      cTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCt                 <  2:4144753/150‑1 (MQ=255)
                                                                                                                                      cTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCt                 <  2:3311741/150‑1 (MQ=255)
                                                                                                                                       tAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTg                >  2:7739655/1‑150 (MQ=255)
                                                                                                                                       tAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTg                <  2:7340568/150‑1 (MQ=255)
                                                                                                                                        aaagagaaagagAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTgg               <  1:1915827/150‑1 (MQ=255)
                                                                                                                                           gagaaagagaaGCCGAGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaa            >  2:7602320/1‑150 (MQ=255)
                                                                                                                                            agaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCaaa           >  1:504802/1‑150 (MQ=255)
                                                                                                                                             gaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAAc          >  1:3696454/1‑150 (MQ=255)
                                                                                                                                             gaaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCCCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAAc          >  2:7162335/1‑150 (MQ=255)
                                                                                                                                              aaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCACACg         >  1:5744676/1‑150 (MQ=255)
                                                                                                                                              aaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACg         >  1:2886931/1‑150 (MQ=255)
                                                                                                                                              aaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACg         >  2:4190970/1‑150 (MQ=255)
                                                                                                                                              aaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACg         >  2:1899688/1‑150 (MQ=255)
                                                                                                                                              aaagagaaGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACg         >  1:1229934/1‑150 (MQ=255)
                                                                                                                                                     aGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACt  >  1:6237667/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCTAAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCATCGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAAACGACAGAAGAGTAATCTCAATGCCAGGTTTAAGCCTGGTATTAAAAGTGCTGGCAAACGCAAAACT  >  NC_000913/2713984‑2714282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑