Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,769,198 T→A 7.1% P178P (CCT→CCA yfjQ → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,769,1980TA 7.1% 1718.4 / 21.6 554P178P (CCT→CCAyfjQCP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (200/297);  new base A (54/2);  total (255/299)
Fisher's exact test for biased strand distribution p-value = 1.07e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 572 total aligned reads displayed.

GTCTGCGGCGAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCT  >  NC_000913/2769049‑2769347
                                                                                                                                                     |                                                                                                                                                     
   tgCGGCGAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCtgtg                                                                                                                                                    <  2:3831513/150‑1 (MQ=255)
   tgCGGCGAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCtgtg                                                                                                                                                    <  2:1827421/150‑1 (MQ=255)
   tgCGGCGAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCtgtg                                                                                                                                                    <  2:7306527/150‑1 (MQ=255)
      ggcgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTgcag                                                                                                                                                 <  2:4442639/150‑1 (MQ=255)
      ggcgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTAACGCCACCTGTgcag                                                                                                                                                 >  2:3695971/1‑150 (MQ=255)
       gcgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTgcagc                                                                                                                                                <  2:7159943/150‑1 (MQ=255)
        cgAGTCGTTTGGCGAGGTGCGGGTGCCACACACGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTgcagca                                                                                                                                               <  2:4624074/150‑1 (MQ=255)
        cgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTgcagca                                                                                                                                               <  2:6952000/150‑1 (MQ=255)
        cgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTgcagca                                                                                                                                               >  2:5991507/1‑150 (MQ=255)
        cgAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCAGTgcagca                                                                                                                                               >  2:6698917/1‑150 (MQ=255)
              gTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACt                                                                                                                                         >  1:4231513/1‑150 (MQ=255)
                ttGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTgg                                                                                                                                       <  1:5088300/150‑1 (MQ=255)
                     gAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCaaaa                                                                                                                                  <  1:2578348/150‑1 (MQ=255)
                     gAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCaaaa                                                                                                                                  <  1:5186333/150‑1 (MQ=255)
                     gAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCaaaa                                                                                                                                  <  2:6213796/150‑1 (MQ=255)
                       ggTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAagc                                                                                                                                <  2:4268431/150‑1 (MQ=255)
                        gTGCGGGTGCCACACCAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAagca                                                                                                                               <  2:6448439/150‑1 (MQ=255)
                        gTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAagca                                                                                                                               >  2:804220/1‑150 (MQ=255)
                         tGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAagcag                                                                                                                              >  1:4008619/1‑150 (MQ=255)
                          gCGGGTGCCACACACGGGGGATGTGGTGCGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAagcagc                                                                                                                             <  2:5667092/150‑1 (MQ=255)
                             ggTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCt                                                                                                                          <  1:6596656/150‑1 (MQ=255)
                                 ccACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACg                                                                                                                      <  1:6321601/150‑1 (MQ=255)
                                  cacacaAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCCGCAGGCACTGGCCAAAGCAGCGCGTACGt                                                                                                                     >  2:5200124/1‑150 (MQ=255)
                                  cacacaAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGt                                                                                                                     >  2:4966388/1‑150 (MQ=255)
                                  cacacaAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTAACGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGt                                                                                                                     >  1:7377534/1‑150 (MQ=255)
                                   acacaAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTa                                                                                                                    >  1:7545334/1‑150 (MQ=255)
                                   acacaAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTa                                                                                                                    >  1:2363727/1‑150 (MQ=255)
                                       aaGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCCAAAGCAGCGCTTACGTATCGc                                                                                                                >  1:6200050/1‑150 (MQ=255)
                                        aGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCt                                                                                                               >  1:7253277/1‑150 (MQ=255)
                                         gggggATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCtt                                                                                                              <  2:7439378/150‑1 (MQ=255)
                                          ggggATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCttt                                                                                                             >  2:3302407/1‑150 (MQ=255)
                                                gtggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAg                                                                                                       <  2:4202858/150‑1 (MQ=255)
                                                gtggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAg                                                                                                       <  2:3492341/150‑1 (MQ=255)
                                                gtggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAg                                                                                                       <  2:3399470/150‑1 (MQ=255)
                                                gtggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTAAGTATCGCTTTGGTGAg                                                                                                       >  2:8112370/1‑150 (MQ=255)
                                                 tggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAATGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTTCGTATCGCTTTGGTGAgg                                                                                                      >  1:6824768/1‑150 (MQ=255)
                                                 tggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAgg                                                                                                      <  1:156083/150‑1 (MQ=255)
                                                  ggtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGa                                                                                                     <  1:5667222/150‑1 (MQ=255)
                                                   gtgAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGac                                                                                                    <  1:1175528/150‑1 (MQ=255)
                                                   gtgAGTCAGGTGTTTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAACGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGac                                                                                                    <  2:2265272/150‑1 (MQ=255)
                                                     gAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGacca                                                                                                  <  1:4972601/150‑1 (MQ=255)
                                                          aGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGc                                                                                             <  2:2472774/150‑1 (MQ=255)
                                                           ggTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGcc                                                                                            >  1:5851545/1‑150 (MQ=255)
                                                           ggTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGcc                                                                                            <  1:3198180/150‑1 (MQ=255)
                                                           ggTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGcc                                                                                            <  1:3548515/150‑1 (MQ=255)
                                                           ggTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTAACGCCACCTGTGCAGCAGGCACAGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGcc                                                                                            >  1:178975/1‑150 (MQ=255)
                                                            gTTATTGAGGGGGCGTATGCAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCg                                                                                           <  1:665327/150‑1 (MQ=255)
                                                             ttATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCgg                                                                                          >  2:1964766/1‑150 (MQ=255)
                                                                ttGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTa                                                                                       >  2:7894245/1‑150 (MQ=255)
                                                                  gAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACt                                                                                     <  1:3525364/150‑1 (MQ=255)
                                                                  gAGGGGGAGTATGAAGTGCTGGGGATTTTTTATCGTGTTTAAGAAAAGAGGGATGCCATTCAGTCGTTGCTTTTACCGCCAACTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTTCGTATCGCTTTGGTGAGGACCACCAGCCGGTTACt                                                                                     <  2:325637/150‑1 (MQ=25)
                                                                     ggggCGTATCAAGTGCTGGGGATTTTTGAGCGGGTGGAAGAAAAGAGGGATGCCATGCAGACGTATCATTTACCCCCACCAGTGCAGCAGGCACAGGCAAAAGCAGCGCTTACTTAAAGCTATGGTGAGGCCCACCAGCCCCTTACTGaa                                                                                  >  2:5835634/1‑150 (MQ=255)
                                                                      gggCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGaaa                                                                                 >  2:2784154/1‑149 (MQ=255)
                                                                          gTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCa                                                                             <  2:1569335/150‑1 (MQ=255)
                                                                            aTGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGa                                                                           <  2:5908192/150‑1 (MQ=255)
                                                                            aTGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGa                                                                           <  1:3976737/150‑1 (MQ=255)
                                                                            aTGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGa                                                                           <  2:7034967/150‑1 (MQ=255)
                                                                                 gTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATActc                                                                      <  1:1962166/150‑1 (MQ=255)
                                                                                 gTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATActc                                                                      <  2:4719178/150‑1 (MQ=255)
                                                                                     tGGGGATTGTTGAGTGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGGTGTTACCCCCACCCGTGCCGCAGGCACGGGCAAAAGCCGCGCGTCAGGATAGGTTTGGTGAGGACCACCACCCCGTTAATGAAAAGCAGATACACTccc                                                                  >  1:3293105/1‑150 (MQ=255)
                                                                                      ggggATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTAACGCCACCAGTGCAGCAGGCACAGGCAAAAGCAGCGCAAAACTATCGCGTTTGTGAGGACCACCACCCCGTTACAGAAACGCACAAACACAcccc                                                                 >  1:3731958/1‑150 (MQ=21)
                                                                                        ggATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCAc                                                               <  2:304384/150‑1 (MQ=255)
                                                                                        ggATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCCCCACCAGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCAc                                                               >  2:1761493/1‑150 (MQ=255)
                                                                                             tttGAGCGTGTGTAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCAcgccgc                                                          <  1:292968/150‑1 (MQ=255)
                                                                                              ttGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCt                                                         <  1:4101195/150‑1 (MQ=255)
                                                                                               tGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTg                                                        <  2:3749304/150‑1 (MQ=255)
                                                                                               tGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTg                                                        <  1:4559815/150‑1 (MQ=255)
                                                                                                 aGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGc                                                      <  2:2839116/150‑1 (MQ=255)
                                                                                                  gCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCa                                                     <  1:6472313/150‑1 (MQ=255)
                                                                                                  gCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCa                                                     <  1:2687919/150‑1 (MQ=255)
                                                                                                  gCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCa                                                     <  2:1697576/150‑1 (MQ=255)
                                                                                                   cGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTTCTTTTACCCCCACCAGTGCAGCAGGCACTGGCAAAAGCACCCCATACGTATAGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACACTCCCCACCCCCCTGGCAg                                                    >  1:5258898/1‑150 (MQ=21)
                                                                                                    gtgtGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATAGCTTTGGTGAGGACCACCAGCCGGTTACTGAATAGCAGATACTAACCCCACGCCGcaggcagg                                                   >  1:8132145/1‑150 (MQ=25)
                                                                                                      gtgGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGAt                                                 <  2:2209563/150‑1 (MQ=255)
                                                                                                       tgGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATg                                                >  2:4823916/1‑150 (MQ=255)
                                                                                                       tgGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTAACGCCACCAGTTCAGCAGGCACAGGGAAAAGCAGCGCTTAAGTATAGCGTTGGTTTGGACCACCACCCCGTTAATGAATCGCAGATAATCTACCCACCCCGCGGGCAGGATg                                                >  1:5966448/1‑150 (MQ=21)
                                                                                                        ggAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATga                                               <  1:7658440/150‑1 (MQ=255)
                                                                                                          aagaaAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATgaga                                             <  1:7828136/150‑1 (MQ=255)
                                                                                                          aagaaAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCGTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATgaga                                             >  2:4019027/1‑150 (MQ=255)
                                                                                                               aaGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAAt                                        >  2:5484398/1‑150 (MQ=255)
                                                                                                                agagGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATg                                       >  2:8222098/1‑150 (MQ=255)
                                                                                                                  agGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGAc                                     <  1:4837687/150‑1 (MQ=255)
                                                                                                                       tGCCATGTAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTg                                >  2:636795/1‑150 (MQ=255)
                                                                                                                       tGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTg                                <  1:859223/150‑1 (MQ=255)
                                                                                                                        gCCTTGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTgg                               <  2:1232597/150‑1 (MQ=255)
                                                                                                                         ccATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGa                              <  1:2099920/150‑1 (MQ=255)
                                                                                                                         ccATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGa                              <  1:4391570/150‑1 (MQ=255)
                                                                                                                            tGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAATAGCAGCGCTTACGTATCGCTTTGGTGAGGACCAGCAGCCGGTTACTGAATCGCAGATACTCTCCACACGCCGCTGTCAGGATGAGAGCAATGACCTTTGGacca                           <  1:6998942/150‑1 (MQ=255)
                                                                                                                            tGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGacca                           <  1:2252489/150‑1 (MQ=255)
                                                                                                                              cAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGATAGCAATGACCTTTGGaccacc                         >  2:5348002/1‑150 (MQ=255)
                                                                                                                              cAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGaccacc                         <  2:658812/150‑1 (MQ=255)
                                                                                                                                        ttttACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGa               >  2:1104246/1‑150 (MQ=255)
                                                                                                                                        ttttACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGa               >  2:7592494/1‑150 (MQ=255)
                                                                                                                                        ttttACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGa               >  1:1344903/1‑150 (MQ=255)
                                                                                                                                         tttACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGAt              >  2:3999669/1‑150 (MQ=255)
                                                                                                                                         tttACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGAt              >  1:5454388/1‑150 (MQ=255)
                                                                                                                                              cGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAg         >  2:768676/1‑150 (MQ=255)
                                                                                                                                              cGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAg         >  2:5107352/1‑150 (MQ=255)
                                                                                                                                               gCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGa        >  1:1027808/1‑150 (MQ=255)
                                                                                                                                                     tgtgCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCt  <  2:2112633/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCTGCGGCGAGTCGTTTGGCGAGGTGCGGGTGCCACACAAGGGGGATGTGGTGAGTCAGGTTATTGAGGGGGCGTATGAAGTGCTGGGGATTTTTGAGCGTGTGGAAGAAAAGAGGGATGCCATGCAGTCGTTGCTTTTACCGCCACCTGTGCAGCAGGCACTGGCAAAAGCAGCGCTTACGTATCGCTTTGGTGAGGACCACCAGCCGGTTACTGAATCGCAGATACTCTCCCCACGCCGCTGGCAGGATGAGAGCAATGACCTTTGGACCACCTATCAGCGGATTCAAGAAAACCT  >  NC_000913/2769049‑2769347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: