BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,817,916	T→G	7.6%	intergenic (‑56/+143)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,916	0	T	G	7.6%	1305.5 / 74.5	408	intergenic (‑56/+143)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base T (179/197); new base G (16/16); total (195/213)
Fisher's exact test for biased strand distribution p-value = 8.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 418 total aligned reads displayed.

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

   ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                                    <  1:1595128/150‑1 (MQ=255)

     ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAaga                                                                                                                                                  <  1:7173382/150‑1 (MQ=255)

     ttACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAaga                                                                                                                                                  <  2:6241659/150‑1 (MQ=255)

       aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGA                                                                                                                                                >  1:4205489‑M1/1‑150 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                             <  1:2655250‑M1/150‑1 (MQ=255)

          gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGG                                                                                                                                             <  1:4946117‑M1/150‑1 (MQ=255)

            gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTG                                                                                                                                           <  2:5040045‑M1/150‑1 (MQ=255)

                         ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTTCTGATATTGATACCGCTTCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAT                                                                                                                              <  2:3128378‑M1/150‑1 (MQ=255)

                            ggaggaTTCGAACCTCCGACCGCTCGGGTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                                                           >  1:5449068/1‑150 (MQ=255)

                                    cGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                   <  1:5612838/150‑1 (MQ=255)

                                      aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAAATAATTCAACGCCGGAAAATCATACCCCGCCGGAGGCCCTAACAAGTAAGAGATGGTGCGTCCTGGACGCTTGCTCGCCTTGCg                                                                                                                 >  2:5434791/1‑150 (MQ=9)

                                       aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCgg                                                                                                                <  1:4059589/150‑1 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             >  2:2376658/1‑150 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             >  2:1261665/1‑150 (MQ=255)

                                          tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTAGTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                             <  2:6823527/150‑1 (MQ=255)

                                              aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTCGTCCTGAAATTCATACCGCTACAAAACCCATAGCAATTAAGAGATGGTGCATCCCGGAGGATTACTCGCCTAGCGGCTCGccc                                                                                                         >  2:4956212/1‑150 (MQ=17)

                                                   tCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                                    <  2:558664/150‑1 (MQ=255)

                                                     ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGc                                                                                                  >  1:6332634/1‑150 (MQ=255)

                                                       ttCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCg                                                                                                >  1:7923664/1‑150 (MQ=255)

                                                          gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATTCATCTGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATAAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                                             <  2:8106738/150‑1 (MQ=255)

                                                          gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                                             <  1:6410426/150‑1 (MQ=255)

                                                          gTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTg                                                                                             <  2:757050/150‑1 (MQ=255)

                                                             gCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTa                                                                                          <  1:7727095/150‑1 (MQ=255)

                                                                 aGTACTCTATCCAGCTGATCTACGTATTCATCGGTAATACTTATTCTACTTATGATATTGATACAGATACTTAAGCCATATATATTATTAGATTGTGCATCCGGGAGTATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGc                                                                                      <  2:3252253/150‑1 (MQ=17)

                                                                   tACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                                                    <  1:1045243/150‑1 (MQ=255)

                                                                   tACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                                                    <  1:2759352/150‑1 (MQ=255)

                                                                    acactATCCAGCTGAGCAACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   <  2:7944781/147‑1 (MQ=255)

                                                                    aCTCTAGCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGAGCCCGCGACTAACGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                                                   <  1:3147702/150‑1 (MQ=17)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCGt                                                                                 >  1:453792/1‑150 (MQ=255)

                                                                      tctATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGt                                                                                 >  1:2920742/1‑150 (MQ=255)

                                                                        tATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTCAGGCAACGTTa                                                                               >  2:2915882/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                                                          >  1:4699426/1‑150 (MQ=255)

                                                                             aGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTatcatc                                                                          >  1:8029507/1‑150 (MQ=255)

                                                                              gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagcc                                                                                                 <  2:5675870‑M1/126‑29 (MQ=255)

                                                                                 gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTa                                                                      <  1:4326230/150‑1 (MQ=255)

                                                                                  aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAg                                                                     >  1:5344258/1‑150 (MQ=255)

                                                                                    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCACTAGTg                                                                   >  2:6117028/1‑150 (MQ=255)

                                                                                     tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGc                                                                  >  1:7794270/1‑150 (MQ=255)

                                                                                     tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGGTCTCATCCCTAGTGc                                                                  >  1:7356561/1‑150 (MQ=255)

                                                                                       cGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCt                                                                >  2:2717915/1‑150 (MQ=255)

                                                                                        ggATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTg                                                               <  1:6725111/150‑1 (MQ=255)

                                                                                           tgctgcGGGAAAACTTATTCTACTGCTTATATTGATACCGCTACTACAGCCATATCAATTTCTAGATGGTGCCTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCgg                                                            <  1:1307357/145‑1 (MQ=18)

                                                                                            gCATCGGGAACACTTAGTCTTCTGCTGATATTGATACCGCTACTAACGCCATATCAATTATGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                           <  1:1158160/150‑1 (MQ=17)

                                                                                            gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTACAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                           <  1:4466464/150‑1 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        <  1:4167211/150‑1 (MQ=255)

                                                                                               tCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTaa                                                        <  1:7248288/150‑1 (MQ=255)

                                                                                                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAtt                                                      <  2:718602/150‑1 (MQ=255)

                                                                                                  ggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTc                                                     <  2:6069929/150‑1 (MQ=255)

                                                                                                   gAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTcc                                                    <  1:5894102/150‑1 (MQ=255)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  2:7710245/150‑1 (MQ=255)

                                                                                                    aaaaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCg                                                   <  1:5664920/150‑1 (MQ=255)

                                                                                                     ataCTTATACTACTGCTGATATTGATACCGCTACTACAGCAATCTCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                  <  1:1228165/148‑1 (MQ=255)

                                                                                                       aCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACAACCAAAGACTCTACCCATTAGGGCCGTTTCTATAGCAACGTTATCATCCCTAGTGCCTGAGGTTATTACCGCTa                                                >  2:2575424/1‑150 (MQ=17)

                                                                                                        tttaTTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggagaggagttga                                               >  2:7030461‑M1/2‑72 (MQ=255)

                                                                                                          tATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgagg                                                     >  1:8111942‑M1/1‑70 (MQ=255)

                                                                                                          tATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgagg                                                     >  2:4486796‑M1/1‑70 (MQ=255)

                                                                                                             tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          >  1:917950/1‑150 (MQ=255)

                                                                                                             tCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCtt                                          <  1:5751322/150‑1 (MQ=255)

                                                                                                              cTACTGCTGATATTGATACCTCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTc                                         <  2:3616418/150‑1 (MQ=255)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTa                                                                                                                            <  1:3413645/66‑1 (MQ=37)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTa                                                                                                                            >  2:3413645/1‑66 (MQ=37)

                                                                                                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                        >  1:6134387/1‑150 (MQ=255)

                                                                                                                    ctgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattc                                            <  1:3570901‑M1/141‑82 (MQ=255)

                                                                                                                     tgATATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattc                                            >  2:4393864‑M1/1‑59 (MQ=255)

                                                                                                                      gATATAGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCCGTATCAACCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCta                                 >  2:1446852/1‑149 (MQ=17)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                             <  1:286073/150‑1 (MQ=255)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                             >  1:5977746/1‑150 (MQ=255)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGATAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGATACCTCTTCGATACCTTCAtt                             >  2:1119259/1‑150 (MQ=255)

                                                                                                                          ttGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtga                                                       <  2:1528929‑M1/124‑71 (MQ=255)

                                                                                                                            gATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAATGc                           >  2:7295286/1‑150 (MQ=255)

                                                                                                                             aTACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaactggcgtggaggcggccattcgaacaacggatgcagctt                          >  2:4871349‑M1/1‑51 (MQ=255)

                                                                                                                               aCCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGa                        <  2:8161494/150‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTTAGAGATGGTGCATCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:2990962/150‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:6925325/150‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGaa                       <  1:5171680/150‑1 (MQ=255)

                                                                                                                                ccGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttg                       <  2:628611‑M1/150‑103 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCg                                                                                                                 >  2:4176499/1‑58 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     >  2:7613120/1‑150 (MQ=255)

                                                                                                                                  gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                     >  1:2365121/1‑150 (MQ=255)

                                                                                                                                   ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAt                    >  2:3507747/1‑150 (MQ=255)

                                                                                                                                     actaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtgaggcggggattcgaaccccggatgcagcttttgaccgc                  <  1:6621318‑M1/150‑108 (MQ=255)

                                                                                                                                      ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                 <  1:3475232/150‑1 (MQ=255)

                                                                                                                                       ttcaGCCATATCTATTATGAGATGGTGCATCCGGGAGGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                <  2:5930393/147‑1 (MQ=255)

                                                                                                                                         aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGAGTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga              <  2:2192771/150‑1 (MQ=255)

                                                                                                                                          aGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtga                                                       <  1:2259454‑M1/108‑71 (MQ=255)

                                                                                                                                          aGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTcgaacctccgaccgctcggttcgtagccgagtactctatccagctgagctacggatgcaaatggcggtga                                                       <  1:69872‑M1/108‑71 (MQ=255)

                                                                                                                                           gCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga            >  2:5114135/1‑150 (MQ=255)

                                                                                                                                            ccATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                                                           <  1:1705104/54‑1 (MQ=255)

                                                                                                                                                atCACTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg       <  1:6567169/150‑1 (MQ=255)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                                                        <  1:3921548/69‑1 (MQ=35)

                                                                                                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATg       <  2:3974677/150‑1 (MQ=255)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCggg                                                                                                   <  1:146700/57‑1 (MQ=35)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg      <  1:5923253/150‑1 (MQ=255)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg      <  1:2020299/150‑1 (MQ=255)

                                                                                                                                                 tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg      >  2:1809685/1‑150 (MQ=255)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg    <  1:2151966/150‑1 (MQ=255)

                                                                                                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTAGCCTAGCGGCTCGCCCTTCTTGCCGTTGCAAAAGAAACGTTATCATCCCTAGTGCCTGCGGTTAATACCGCTAACAATTCGTAACCTTCATAGCTGAAGATTTCGGAGAATATGGTg    >  2:166189/1‑150 (MQ=17)

                                                                                                                                                    aTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCg                                                                                                            <  1:1852694/45‑1 (MQ=35)

                                                                                                                                                     ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGc                                                                                                           >  2:649437/1‑45 (MQ=32)

                                                                                                                                                     ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa  >  2:4572635/1‑150 (MQ=255)

GAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCA  >  NC_000913/2817767‑2818065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`