Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,075 A→C 5.7% intergenic (‑174/+93) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0750AC 5.7% 1310.5 / 13.1 412intergenic (‑174/+93)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base A (146/228);  new base C (29/2);  total (177/235)
Fisher's exact test for biased strand distribution p-value = 1.17e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

GTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAAC  >  NC_000913/3045927‑3046224
                                                                                                                                                    |                                                                                                                                                     
gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCaa                                                                                                                                                      >  2:4660254/1‑150 (MQ=255)
 ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCGGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                     <  2:1026737/150‑1 (MQ=255)
 ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                     <  2:3443172/150‑1 (MQ=255)
    aCGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                                                  >  1:8003927/1‑150 (MQ=255)
     cGTATCTTTGAGCTTGCTAACACCCTGGCTCAATCTTCAATGTTGGCACGCTTGTCCCCCTCGCCCGTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTGCCCCTCACCCTAACCCTCTCCCCAAAgggg                                                                                                                                                 <  2:1752551/150‑1 (MQ=37)
      gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                                                >  2:5929060/1‑150 (MQ=255)
        aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                              <  1:1663027/150‑1 (MQ=255)
        aTCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                                              <  1:6813238/150‑1 (MQ=255)
         tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                                             <  1:1209044/150‑1 (MQ=255)
          cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                                            <  1:3161832/150‑1 (MQ=255)
            ttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCGAGTGCCGGCCTGGTCCCCTCACCCTAACCCGCTCCCCGCAGGGGCgggggg                                                                                                                                          >  2:6426678/1‑150 (MQ=255)
             tGAGATTGCTCACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                                         <  2:2087776/150‑1 (MQ=255)
             tGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCGAggggc                                                                                                                                         >  1:5160310/1‑149 (MQ=255)
               agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGCACCCGCACCCCAAAGGGGCGAGGGGGCt                                                                                                                                       >  1:2595498/1‑150 (MQ=255)
                     cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                                 <  1:632179/150‑1 (MQ=255)
                     cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                                 <  2:4801964/150‑1 (MQ=255)
                     cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                                 <  2:2064281/150‑1 (MQ=255)
                     cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                                 <  1:6134275/150‑1 (MQ=255)
                     cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                                                 <  2:3019463/150‑1 (MQ=255)
                       aaCAAAATGGATAAATCTTCAAACTTGTAAAGCTTTTCCACCTCGACCTATCAGTGAGAGGGCCGGGGTGAGGGTAAATATTCGCGACAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCACCACAAATGGGCGAGGTGAATGTATTAGc                                                                                                                               >  2:1194722/1‑150 (MQ=16)
                          aaaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCTCACCCCACAGGGGCGCGGGGCCTGTCTGAGCCCt                                                                                                                            >  2:7106822/1‑150 (MQ=255)
                           aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtt                                                                                                                           >  2:4582417/1‑150 (MQ=255)
                           aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGCGGGGACTGTCTGAGCAcgt                                                                                                                           >  2:4462154/1‑148 (MQ=255)
                             cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                                                         <  2:3414087/150‑1 (MQ=255)
                             cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                                                         <  2:6025877/150‑1 (MQ=255)
                             cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                                                         <  2:5852007/150‑1 (MQ=255)
                                gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTa                                                                                                                      <  1:500059/150‑1 (MQ=255)
                                 cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGCCTGTCAGAGCACTTTTGTAc                                                                                                                     >  2:2001371/1‑150 (MQ=255)
                                   cAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACtt                                                                                                                   <  2:4219574/150‑1 (MQ=255)
                                    aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                                  <  1:864065/150‑1 (MQ=255)
                                    aaTCTTCAATGGGGGCACGCTTTTCCCCCGCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAACTCGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                                                  <  1:7693341/150‑1 (MQ=25)
                                     aTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAAACCACACCCCAAAGGGGCGAGGGGACTGTCTGAGCACATTTGTAAtttt                                                                                                                 >  2:1823414/1‑149 (MQ=37)
                                       cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                                               <  2:3352358/150‑1 (MQ=255)
                                        ttCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACAACCCAAAGGGGCGAGGGGGCTGTCTGAGCACTTTTTTACATTTTCa                                                                                                              >  1:4510300/1‑150 (MQ=25)
                                          cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                                            <  2:410462/150‑1 (MQ=255)
                                           aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                           <  2:6626524/150‑1 (MQ=255)
                                           aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                           <  1:5354572/150‑1 (MQ=255)
                                           aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                                           <  2:3146453/150‑1 (MQ=255)
                                             tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                                         <  1:555279/150‑1 (MQ=255)
                                             tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                                         <  2:7598894/150‑1 (MQ=255)
                                               ttGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACAACCCAAAGGGGCGGGGGGGCGGTCTGGGCACATTTGTAATTTGTCaaaagact                                                                                                       >  1:1000715/1‑143 (MQ=255)
                                                    aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                                                  <  1:276284/150‑1 (MQ=255)
                                                          ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGcc                                                                                            <  1:7420265/150‑1 (MQ=255)
                                                          ttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCGAACCCGCTCCCCAAAGGGGCGCGGGGACGGTCTGAGCACGGTTGTACTGTGGCATCTGACAAAAAAGGCGcc                                                                                            >  2:7864225/1‑150 (MQ=255)
                                                              cccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCACCCCCAAGGGGCGAGGGGGCTGTCTGAGCACGTTTGTACGTTGTCATCTGACTAAAAAGGCGCCGAAg                                                                                        >  2:7824239/1‑150 (MQ=37)
                                                               ccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAAGATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgc                                                                                       <  2:5563662/150‑1 (MQ=255)
                                                                cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCGAAACCACTACCCCAATGGGGGAGGGGACTGTCAGAGCACTTTTGTTCTGTTTGATCAGTCAAAAAAGGCGCCGCAgcg                                                                                      >  2:6445829/1‑150 (MQ=255)
                                                                 tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                                                     <  2:2253697/150‑1 (MQ=255)
                                                                 gcGCCCTTTCCGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                                                     <  1:6425567/149‑1 (MQ=255)
                                                                       tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCAAATGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                                               >  2:5004417/1‑150 (MQ=255)
                                                                        ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGGGCCCGCACCCCAACCCGCACCCCCAAAGGGGGAGGGGGCGGGCTGAGCACATTTGTTGATTTTCATAAGAATAAAAAAGCGCCGGAGCGCCCTgaga                                                                              >  1:2938434/1‑150 (MQ=255)
                                                                           aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                                           <  2:6941811/150‑1 (MQ=255)
                                                                             ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                         <  1:5271985/150‑1 (MQ=255)
                                                                             ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                                         <  1:2434943/150‑1 (MQ=255)
                                                                             ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCAACCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACGTTGTCATCTGACTAAAAAGGCGCCGCAGCGCCTTTAGAAAATa                                                                         >  2:4394458/1‑150 (MQ=37)
                                                                                gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                                                      <  1:4338806/150‑1 (MQ=255)
                                                                                gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTc                                                                      <  1:3760629/150‑1 (MQ=255)
                                                                                    gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTGTAGAAAATAGTCGaaa                                                                  >  2:4171644/1‑149 (MQ=255)
                                                                                     ccGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCTCTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTTTACATTGTCATCTGACAAAAAATGCGCCGAAGCGCCTTTATAAAAAAGTCGAATc                                                                 >  2:1685705/1‑150 (MQ=37)
                                                                                               ggTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAc                                                       <  2:8069459/150‑1 (MQ=255)
                                                                                               ggTAAAGCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAc                                                       <  2:5203076/150‑1 (MQ=255)
                                                                                                gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                                                      <  2:2747415/150‑1 (MQ=255)
                                                                                                 tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                                                     <  1:4117797/150‑1 (MQ=255)
                                                                                                  aaaTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTgg                                                    <  2:8218700/150‑1 (MQ=255)
                                                                                                   aaTATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCGAAACCACAACCCCAAGGGGCGGGGGGACTGTCTGAGCACGTTTGTACTTTGTCAACTGACTAAAAATGCGCCCCAGCGCCATTAGAAAATAGTCGAATCACTGAATTACTGGt                                                   >  2:5635955/1‑150 (MQ=255)
                                                                                                       ttCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAATGGTATTc                                               >  2:6864278/1‑150 (MQ=255)
                                                                                                        tCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                                              <  2:3867382/150‑1 (MQ=255)
                                                                                                         cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                                             <  2:8183541/150‑1 (MQ=255)
                                                                                                         cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                                             <  1:1299237/150‑1 (MQ=255)
                                                                                                             ccAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCAGAGCACTTTTGTACTTTGTCATCAGACTAAAAAGTCGCCGAAGCGCCTTTAGAAAATATTCGAAACAGTGAATTAATGGTATTAGCTAAt                                         >  2:1345177/1‑150 (MQ=38)
                                                                                                              cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc                                        <  1:1805574/150‑1 (MQ=255)
                                                                                                              cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc                                        <  2:3704154/150‑1 (MQ=255)
                                                                                                              cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc                                        <  1:1423268/150‑1 (MQ=255)
                                                                                                              cAGTGCCGGCCTGTTCCCCTCACCCTAACCCACAACCCAAAGGGGCGAGGGGAATGTCAGAGCACATTTTTACTTTGTCAACTGACTAAAAATGCGCCGAAGCGCCGTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc                                        >  2:7938443/1‑150 (MQ=38)
                                                                                                                gTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCgg                                      <  1:6255162/150‑1 (MQ=255)
                                                                                                                    cGGCCTGGTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACg                                  <  1:1710069/150‑1 (MQ=255)
                                                                                                                       ccTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAg                               <  2:3805948/150‑1 (MQ=255)
                                                                                                                         tGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa                             <  1:2105820/150‑1 (MQ=255)
                                                                                                                         tGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa                             <  1:5615274/150‑1 (MQ=255)
                                                                                                                             ccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAg                         >  2:2974310/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGaa                       >  1:3490815/1‑150 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAAc                      <  1:4724419/150‑1 (MQ=255)
                                                                                                                                 tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACa                     <  1:7072678/150‑1 (MQ=255)
                                                                                                                                 tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACa                     >  2:3634646/1‑150 (MQ=255)
                                                                                                                                 tCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACa                     <  2:307394/150‑1 (MQ=255)
                                                                                                                                  cACCCGAACCCTCTCCCCAACGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAg                    <  1:4039154/150‑1 (MQ=255)
                                                                                                                                      cTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTGTAGAAAATAGTCGAATCAGTGAATTACTGGTCTTCGCTAATCGGTACGCAGGAGCAGAACAGGTTa                >  1:6565881/1‑150 (MQ=255)
                                                                                                                                        aaCCCTCTCCCCCAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACg              <  2:4864513/150‑1 (MQ=255)
                                                                                                                                          cccTCGCCCCTTTGGGGAGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTCATCGGTACGCAGGAGCAGAACAGGTTACGGt            >  2:6265047/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTc           >  2:2477146/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCg          >  1:2188496/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc         >  1:3371958/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGc         >  2:2869115/1‑150 (MQ=255)
                                                                                                                                               tcCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATAACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCg       >  2:1427336/1‑150 (MQ=255)
                                                                                                                                                 cccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTa     >  2:7484625/1‑150 (MQ=255)
                                                                                                                                                  ccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaa    >  1:3869713/1‑150 (MQ=255)
                                                                                                                                                  ccAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaa    <  1:5752193/150‑1 (MQ=255)
                                                                                                                                                  ccAAAGGGGCGAGGGGACTGTCTGAGCACTGTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaa    >  2:6179523/1‑150 (MQ=255)
                                                                                                                                                   cAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaaa   <  2:7221574/150‑1 (MQ=255)
                                                                                                                                                   cAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTaaa   <  2:2382655/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGCAGAACAGGTTACGGTCGCCGTAAAC  >  NC_000913/3045927‑3046224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: