Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,609 T→G 7.0% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6090TG 7.0% 877.5 / 22.3 297intergenic (+64/‑335)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (196/63);  new base G (4/16);  total (201/96)
Fisher's exact test for biased strand distribution p-value = 9.01e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757
                                                                                                                                                       |                                                                                                                                                    
 gggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTc                                                                                                                                                     <  1:7907234/150‑1 (MQ=255)
 gggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTc                                                                                                                                                     >  2:6221091/1‑150 (MQ=255)
 gggAGTGGTGTTTGGCATTCTGGTGATGACGTTTATTTTCTACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTc                                                                                                                                                     <  2:1700558/150‑1 (MQ=255)
  ggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                                                                                                                                                    >  2:3555829/1‑150 (MQ=255)
   gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                   <  1:4767966/150‑1 (MQ=255)
      tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                >  1:5378759/1‑150 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               <  2:3854588/150‑1 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgag                                                                                                                                              >  2:458059/1‑150 (MQ=255)
         tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             >  1:4026336/1‑150 (MQ=255)
          gtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGagag                                                                                                                                            >  1:5653718/1‑150 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           >  2:6201906/1‑150 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTGTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           >  2:7841249/1‑150 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         >  2:1625122/1‑150 (MQ=255)
              ggCATTCTGGTGATGACGTTTATTATCACCGCCTGGGGGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTc                                                                                                                                        >  2:634635/1‑150 (MQ=255)
               gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                                                                                       >  1:5971898/1‑150 (MQ=255)
                   tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGTATATTTTTTAATCTGCATAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                   <  1:1998249/150‑1 (MQ=255)
                   tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                   >  2:338264/1‑150 (MQ=255)
                     tGGTGATTACGTTTATTATCACCGCGTGGGTGATTTATCGGCATTATATGCTGGGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTAATTAAATTGAGCACGTTTTTCTCCCTCTCCCTTCCTGGGAGAGGGTCGGGGTGa                                                                                                                                 <  1:828727/150‑1 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 <  2:2984866/150‑1 (MQ=255)
                       gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                               <  2:520873/150‑1 (MQ=255)
                         gatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGt                                                                                                                             >  2:1032491/1‑150 (MQ=255)
                           tgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTaa                                                                                                                           <  2:2713157/150‑1 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  1:3492005/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  1:7584144/1‑150 (MQ=255)
                                tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      >  1:7032551/1‑150 (MQ=255)
                                tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      >  2:2236859/1‑150 (MQ=255)
                                 tttttatCTCCGCGTGGGTTATTTATCGGATTGTTAAGCTGGGGGGTTTATATTTTTTATACTGACAACGCTGTTTACCCTGTATTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGTGAGGGTGGGGTTGAGGTTAATTTTTc                                                                                                                     <  2:4707837/147‑1 (MQ=255)
                                 ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                                                                                     >  1:1822679/1‑150 (MQ=255)
                                 ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                                                                                     >  2:4996702/1‑150 (MQ=255)
                                  tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                    >  2:6866290/1‑150 (MQ=255)
                                    ttatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCa                                                                                                                  >  1:6241951/1‑150 (MQ=255)
                                       tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTTGCACCg                                                                                                               >  1:3731897/1‑150 (MQ=255)
                                       tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                               >  2:4463632/1‑150 (MQ=255)
                                        cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                                                                                              >  1:1242520/1‑150 (MQ=255)
                                        cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                                                                                              >  1:407589/1‑150 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATACGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                             >  2:7945028/1‑150 (MQ=255)
                                          aCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATg                                                                                                            >  1:4961318/1‑150 (MQ=255)
                                               tGGGTTATTTATCGGCTTGTTAAGCTGGGGGGTGTATAATTTTTAATCTGCCTACGCGGTGTACCCTGTCTTTAACTTGCGCACCTTTTTCCCCCTCTCCCTCCCCGGGTTTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGc                                                                                                       <  2:4286679/150‑1 (MQ=255)
                                               tGGGTTAATTATCGGCATGATACGCAGGGGGGTGGATAATTTTTAATCTGCCTACGCCGTGTACCCTGTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGc                                                                                                       <  2:2788962/150‑1 (MQ=255)
                                                 ggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                     >  2:1051443/1‑150 (MQ=255)
                                                 ggTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                     >  2:2908065/1‑150 (MQ=255)
                                                  gTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTg                                                                                                    >  1:4383696/1‑150 (MQ=255)
                                                     aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGGCTGTTc                                                                                                 >  1:4307685/1‑150 (MQ=255)
                                                     aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCCTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                 <  2:7674543/150‑1 (MQ=255)
                                                        tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              >  1:6736965/1‑150 (MQ=255)
                                                          tCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTc                                                                                            >  2:6669535/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  1:6192131/1‑150 (MQ=255)
                                                              cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAccc                                                                                        >  2:1383646/1‑150 (MQ=255)
                                                                tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                      >  1:4247697/1‑150 (MQ=255)
                                                                tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                      >  1:663493/1‑150 (MQ=255)
                                                                 gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGAACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGCGAGGGGCGGGGTGAGGGTAATTTTTCGCACCGAGGCTGGCCTGTTCCCCTCCCCCTaa                                                                                     >  2:6052826/1‑150 (MQ=255)
                                                                       cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                               >  1:8008999/1‑150 (MQ=255)
                                                                       cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                               >  1:606668/1‑150 (MQ=255)
                                                                        aggggggTGTATAATTTTTAATCTGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGTAGAGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                              <  2:7405739/147‑1 (MQ=38)
                                                                        agcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCAAACCCactc                                                                              >  1:3668609/1‑150 (MQ=255)
                                                                          cGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCTAAACCGCTccc                                                                            >  2:3597979/1‑150 (MQ=255)
                                                                           ggggTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                           >  1:4668720/1‑150 (MQ=255)
                                                                               tgtttattttttAATCTGCCTAGGCCTTTTACCCTTTATTTAACTTGAGCACGGTTTTCTCCCTCTCCATTCCTGGGTGTGGGTGGGTGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc                                                                       <  2:1905501/143‑1 (MQ=21)
                                                                                    attttttAATCGGCCTACGCGGGGTACCCTTTAATTAACATGAGCACGTTTTTCTCCCTCCCCCTTCCCGGGTGTGGGTGGGGGTTAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                  <  1:5879549/148‑1 (MQ=21)
                                                                                    aaTTTTTAATTCTGCCTCAGCGTTGTACCATGTCATTAACAGGACCCCGTTTTTCTCCCGCCCCAGGCCTGGGTGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   <  2:5833978/150‑1 (MQ=16)
                                                                                      tttttATTCTGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGTAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  1:3397951/150‑1 (MQ=37)
                                                                                      tttttACTCTGCCTAAGCCGTGTACCCTTTCTTAAAATTGCGCACCTTTTTCTCCCTCTCCCTCCCCGTGAGTGGGGGGGGTTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  2:80667/150‑1 (MQ=21)
                                                                                      tttttAATCTGCATACGCGGTGTACCTGGTCTTTAACATGAGCCCGTTTTTCCCCCTCCCCCTCCCCGGGCGGGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  2:1850405/150‑1 (MQ=21)
                                                                                      tttttAATCGGCCTAAGCCGTGTACCCGGTCATTAACTTGAGCACCGTTTTCCCCCCCGCCCTGCCTGGTAGAGGGACGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                <  2:4908928/150‑1 (MQ=25)
                                                                                           aaTCTGCCTAAGCCGTGACCCCTGTCATTAACTTGCGCACGGTTTTCGCCCTCTCCCTTCCTGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGa                                                           <  1:2575516/150‑1 (MQ=25)
                                                                                              tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACt                                                         >  1:3778677/1‑150 (MQ=255)
                                                                                                tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                       >  1:457100/1‑150 (MQ=255)
                                                                                                 gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCGCCCTTCCCGGTAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAc                                                      <  2:1199052/150‑1 (MQ=255)
                                                                                                  ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  1:7316998/1‑150 (MQ=255)
                                                                                                  ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  1:4833351/1‑150 (MQ=255)
                                                                                                   cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                    >  2:5461447/1‑150 (MQ=255)
                                                                                                   cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGCGGTGAGCGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACACGGGGCGAGGGGCCGGACCg                                                    >  1:8109065/1‑150 (MQ=37)
                                                                                                    tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   <  1:6878174/150‑1 (MQ=255)
                                                                                                            gtACCCGGTCATTAACAGGCGCCCCGTTTTCCCCCTCGCCCTGCCCGGGTGTGGGTGGGGGTGAGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                           <  2:7032566/150‑1 (MQ=21)
                                                                                                              aCCCTGTCATTAACCTGAGCACCGTTTTCGCCCTCGCCCGTCCTGGGTGTGGGTCGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  1:1021726/150‑1 (MQ=25)
                                                                                                                ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                       >  2:3245540/1‑150 (MQ=255)
                                                                                                                    tCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                   >  2:7569900/1‑150 (MQ=255)
                                                                                                                       ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                >  2:7221604/1‑150 (MQ=255)
                                                                                                                         aaCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                              >  2:8133822/1‑150 (MQ=255)
                                                                                                                          aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                             >  1:1870650/1‑150 (MQ=255)
                                                                                                                          aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATGTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                             >  2:2641138/1‑150 (MQ=255)
                                                                                                                             tGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                          >  2:921552/1‑150 (MQ=255)
                                                                                                                             tGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                          >  2:2060672/1‑150 (MQ=255)
                                                                                                                                  cccGTTTTCCCCCCTCCCCCTCCCGGGGTGTGGGTGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                      <  1:2908065/149‑1 (MQ=25)
                                                                                                                                  aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                     >  2:3037777/1‑150 (MQ=255)
                                                                                                                                   ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                    >  1:638764/1‑150 (MQ=255)
                                                                                                                                   ccGTTTTCCCCCTCCCCCTCCCCGGGTGTGGGTGGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                    <  1:6776838/150‑1 (MQ=25)
                                                                                                                                    cGTTTTCTCCCTCTACCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACACCCCAAAAGGGGCGAGGGGACTGACCGAGTCCTTTTTTTTTGTTGTAATCAGTATGGAAg                   >  1:1658162/1‑150 (MQ=25)
                                                                                                                                         tctccctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa              >  2:6586684/1‑150 (MQ=255)
                                                                                                                                         tctccctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACAGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa              >  2:295373/1‑150 (MQ=255)
                                                                                                                                           ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc             >  1:7268246/1‑150 (MQ=255)
                                                                                                                                              cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCAGGAAGCCGCACGtt          >  2:7329263/1‑150 (MQ=255)
                                                                                                                                               ctctccctTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         <  2:5380505/150‑1 (MQ=255)
                                                                                                                                               ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         >  2:5092693/1‑150 (MQ=255)
                                                                                                                                               ctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGGGTGTTGCGCTCACCCTATCCGTCTCCGCAATCGGGGCGAGGGGCCTGACGGAGTGCTTTTTTGATGTTGTGATCGGTGTGGAGGCCggtggttg         >  2:5022122/1‑143 (MQ=255)
                                                                                                                                                tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:7136879/1‑150 (MQ=255)
                                                                                                                                                tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  1:7068657/1‑150 (MQ=255)
                                                                                                                                                tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTTTAATCAGTCTGGAAGCCGCACGTTgg        >  2:201268/1‑150 (MQ=255)
                                                                                                                                                  tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCGAACCCACACCCCAAACGGGGCGAGGGGACGGACCGAGTCCATTTTTTATGGTGTCCTCAGTCTGGAAGCCGCACGGTGGCt      >  1:2150411/1‑150 (MQ=255)
                                                                                                                                                   ccctGCCAGGGCGTGGGTGGGGGTGAGGTTAATTGTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGTGGGGCCTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCtt     <  1:3260251/150‑1 (MQ=37)
                                                                                                                                                       |                                                                                                                                                    
TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: