Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,535 G→T 6.6% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5350GT 6.6% 957.9 / 5.8 332H68N (CAT→AAT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (206/89);  new base T (8/27);  total (215/117)
Fisher's exact test for biased strand distribution p-value = 9.77e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238386‑238684
                                                                                                                                                     |                                                                                                                                                     
   tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                                    >  2:379256/1‑150 (MQ=255)
   tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                                    >  1:3110941/1‑150 (MQ=255)
   tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                                    >  2:7476639/1‑150 (MQ=255)
       acaacaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                <  1:570372/150‑1 (MQ=255)
       aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                                                <  1:4742353/150‑1 (MQ=255)
         aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATTTCTGTGGCGAAATTGACTACCTTCGATTTTTTGATTAAGAATGATTTTATTATCGTAATAAAAATTAAATGAATATTTaa                                                                                                                                              >  1:3328873/1‑150 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:7583989/150‑1 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:1403733/150‑1 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  2:659233/150‑1 (MQ=255)
            aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                                           <  1:1936734/150‑1 (MQ=255)
                gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                       >  1:962837/1‑150 (MQ=255)
                gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                       >  1:5238249/1‑150 (MQ=255)
                 cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                      >  1:5917153/1‑150 (MQ=255)
                 cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                      >  2:1885338/1‑150 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATTATTTTATTTTATTAATTAAAATTAAAATAAAATTTaaaatttaaaa                                                                                                                                     >  1:3749416/1‑143 (MQ=255)
                  tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaaa                                                                                                                                     <  1:6302273/150‑1 (MQ=255)
                    ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAAACg                                                                                                                                   >  1:6669692/1‑150 (MQ=255)
                    ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACg                                                                                                                                   >  1:82728/1‑150 (MQ=255)
                      aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                                 >  2:2068473/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTTAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             >  1:2313598/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTACGTAAAATTACATGAATATTTAAAAAGGAATACGACATGa                                                                                                                             >  2:7818022/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             >  1:133650/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                             >  1:4487447/1‑150 (MQ=255)
                          gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGa                                                                                                                             >  2:6442811/1‑150 (MQ=255)
                              tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                         >  1:7213731/1‑150 (MQ=255)
                               aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                                        >  1:1281097/1‑150 (MQ=255)
                               aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                                        >  2:5981460/1‑150 (MQ=255)
                                ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                                       >  2:5345090/1‑150 (MQ=255)
                                ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                                       >  2:1418793/1‑150 (MQ=255)
                                     aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                                  >  2:524331/1‑150 (MQ=255)
                                         cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                              <  2:6044120/150‑1 (MQ=255)
                                          tATTAGTAAAAATATAAGAGTTAGATGAATTTTTATGTCTGTGGCGAAATTGACTACCAACGTATTGTTGTTTAAGAATGAATTTAATATCGTAAATAGAAGTAAATGTCTATTTAAAAATGAAAAACAAAAGAAAACGAATCACAGaaa                                                                                                             >  2:6991029/1‑149 (MQ=255)
                                           aTTAGTAAAAATATATGAGTTAGCTGTATTGTTATGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCTTAAGTAAAATTACTTGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                            <  2:6266357/150‑1 (MQ=255)
                                             tttaaaaaaTATATGTGTTTCTTGAATTTTTTTGTCTGTGGATAATTTCCCTCCATTTGTTTTTTTGATTAATTATTTTTTTATTATCTTAATTAAATTTAAATTAAATTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                          <  1:2009811/146‑1 (MQ=255)
                                             tacaaaaaaaaaaaCAGTTACATCTATTTTAATTTCTGTGCCCAAATTCACAACATAAGTTTTTTTGATTAACAATGATATTATTATATTAATTAAAATTACATTAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                          <  2:8094823/146‑1 (MQ=255)
                                             tAGTAAAAATATAAGAGTTAGCTGTTTTTTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATTATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                                                          <  2:5591051/150‑1 (MQ=255)
                                                tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                                                       >  2:2504673/1‑150 (MQ=255)
                                                    aaTATACTAGTTAGCTGTATTGTTATTTCTGTGGATAAATTGACTACCTTCGTTTTTTTTTTTAATATTGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                   <  1:417637/150‑1 (MQ=255)
                                                      tataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAAAATCAACATTATCCa                                                                                                 >  1:5279321/1‑150 (MQ=255)
                                                       ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                                >  2:6003998/1‑150 (MQ=255)
                                                        taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAATCGACATGAAACCGAAGCACAGAATCAACATTATCCAAt                                                                                               >  2:1699310/1‑150 (MQ=255)
                                                               tAGATGTTTTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATGATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaaa                                                                                        <  1:6490436/150‑1 (MQ=255)
                                                                 gCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGGTTAATAATGATTTTATTATAGTAATAAAAAATAAATTAAAATTTAAAAAAGAAAAAGAAAAGAAAACCAAGCACAAAAACAACAAACTCAAATAAAAAAata                                                                                      >  1:6002787/1‑150 (MQ=255)
                                                                     tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAAATACAAGAATATTTAAAAATGAAAAAGACATGAAACCGAAGCACACAATCAAAAATAAACAATCaaaaaaaaatta                                                                                  >  1:1641914/1‑144 (MQ=255)
                                                                      aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTATCCAATCATAAAATATTTcc                                                                                 >  2:113425/1‑150 (MQ=255)
                                                                       ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGAAATGAAACCCAAGCACAGAAAAAAAAATATACAAAAATAAAAAATTTCCg                                                                                >  1:5890370/1‑150 (MQ=255)
                                                                          ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTATGTATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAATGGAAATCGTCATGAAACCGAAGCTCAGAATCAAGATTCTGCTATCATATAATTTTTGGGTgg                                                                             >  2:4374835/1‑150 (MQ=255)
                                                                                 tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGTGGAGCAttt                                                                      >  2:2963627/1‑150 (MQ=255)
                                                                                 tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                      >  2:3967024/1‑150 (MQ=255)
                                                                                 tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                                      >  2:725410/1‑150 (MQ=255)
                                                                                 tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAATATTTTATTATAGTAAGTAAAATTACATGAAAATTTAAAAAGGAAAACGAAAATAAAACCAAGCACAGAAACAACAAAATCCAAAAAAAAAAAATTTAAGTGGAGCAttt                                                                      >  1:1020761/1‑150 (MQ=255)
                                                                                  gtgGCGAAATTTACTACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtttt                                                                     <  1:989064/150‑1 (MQ=255)
                                                                                   tgGCGAAATTGACTACCTTCGTGTTTTTGTTTAATAATTTTTTTATTATAGTAAGTAAAAATAAAAGAATATTTTAAAAGGAAAACCACAAGAAAACCCAGCACACAAAAAACAATATCCAAAAATAAAAAATTTACGTGGAGCATTtta                                                                    >  1:3905064/1‑150 (MQ=255)
                                                                                     gagAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                                  <  2:3399541/148‑1 (MQ=255)
                                                                                        aaaTTTACTAACTTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:7382854/150‑1 (MQ=255)
                                                                                        aaaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:4169259/150‑1 (MQ=255)
                                                                                        aaaTTGACTACCTACGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                               <  2:4671384/150‑1 (MQ=255)
                                                                                          aTTGACGACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                             <  1:7415664/150‑1 (MQ=255)
                                                                                            tGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAat                                                           >  2:1381127/1‑150 (MQ=255)
                                                                                                tACCTTCGTTTTTTTGATTAAGATTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                                       <  2:2576987/150‑1 (MQ=255)
                                                                                                tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAATTCCGTGGAGCATTTTATTATTGAAtatat                                                       >  2:450637/1‑149 (MQ=255)
                                                                                                  ccTTCTTTTTTTTTATTAATAATTATTTTATTATCGTAAGTAAAATTACATTAATTTTTAAAAAGGAACACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  1:2611990/150‑1 (MQ=255)
                                                                                                  aattCTTTTTTTTGTTTAATAATGTTTTTTTTATCTTATTTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCTAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                     <  2:3749416/148‑1 (MQ=255)
                                                                                                   cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                                                    >  2:2370452/1‑150 (MQ=255)
                                                                                                   cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAgg                                                    >  2:1599152/1‑150 (MQ=255)
                                                                                                   cTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCACACAATCAACAATCTCCAATCATAAAATATTTAAGTGGAGCATTTTATTATTGAATATATAgg                                                    >  1:5579116/1‑150 (MQ=255)
                                                                                                    ttCGTTTTTTTGATTAATAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                                                   <  1:5042811/150‑1 (MQ=255)
                                                                                                     tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAACCGAAGCACAGAATAAAAAATATACAATAAAAAAATATTTACGTGGAGCATTTTATTATTGAATATATAGGtt                                                  >  1:2763516/1‑150 (MQ=255)
                                                                                                     tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                                  >  2:8245010/1‑150 (MQ=255)
                                                                                                      cGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                                 <  2:263498/150‑1 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAATCGACATGAAACCGATGCACAGAATCAACATTCTCCAATCATAATATATTTCGGTGGAGCATTTTTTTATTGTTTATATAGGtttttgtc                                            >  2:7182101/1‑145 (MQ=255)
                                                                                                           ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                            >  2:7750351/1‑150 (MQ=255)
                                                                                                            tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           >  1:2378146/1‑150 (MQ=255)
                                                                                                            tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                           >  2:7131806/1‑150 (MQ=255)
                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                       >  2:7359249/1‑150 (MQ=255)
                                                                                                                aTTAAGAATGATTTTATTATCGTAAGTAAAATTACAGGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTAGTGAATATAGAGGTTTAACTCCGGtc                                       >  1:6763490/1‑149 (MQ=255)
                                                                                                                 ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                                      >  2:4915746/1‑150 (MQ=255)
                                                                                                                  tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCTGGTaaa                                     >  1:6985995/1‑150 (MQ=255)
                                                                                                                    aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGAACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                                   >  1:2717730/1‑150 (MQ=255)
                                                                                                                     taaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                                  <  2:3150212/149‑1 (MQ=255)
                                                                                                                      aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                                 >  1:1099802/1‑150 (MQ=255)
                                                                                                                          aTTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                             <  2:5682174/150‑1 (MQ=255)
                                                                                                                           ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                            >  2:6167896/1‑150 (MQ=255)
                                                                                                                                    cGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGaa                   >  1:5736677/1‑150 (MQ=255)
                                                                                                                                     gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                  >  2:1477210/1‑150 (MQ=255)
                                                                                                                                     gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt                  >  2:5148398/1‑150 (MQ=255)
                                                                                                                                        attaaAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa               <  1:2123943/148‑1 (MQ=255)
                                                                                                                                         ttaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  1:4674821/149‑1 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  1:7363758/150‑1 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              >  2:4775267/1‑150 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              <  2:5937991/150‑1 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              >  2:679693/1‑150 (MQ=255)
                                                                                                                                         gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg              >  1:4945176/1‑150 (MQ=255)
                                                                                                                                          taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg             <  2:3481248/150‑1 (MQ=255)
                                                                                                                                          taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGAAgg             >  1:7972096/1‑150 (MQ=255)
                                                                                                                                               ttACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaa        >  1:1443758/1‑150 (MQ=255)
                                                                                                                                                tACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa       <  1:2456808/150‑1 (MQ=255)
                                                                                                                                                 aCATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaat      <  1:5176514/150‑1 (MQ=255)
                                                                                                                                                  cATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaata     <  1:1750554/150‑1 (MQ=255)
                                                                                                                                                   aTGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaataa    >  1:3511980/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATA  >  NC_000913/238386‑238684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: