Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,220 C→G 5.3% L132V (CTG→GTG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2200CG 5.3% 1395.7 / 51.4 391L132V (CTG→GTG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (210/159);  new base G (10/11);  total (221/170)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 395 total aligned reads displayed.

CTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTT  >  NC_000913/3368072‑3368369
                                                                                                                                                    |                                                                                                                                                     
  ctGCAATAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTAACACCATTGGGACTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCGGACGCTGt                                                                                                                                                    >  2:6695137/1‑150 (MQ=255)
   tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGACTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                   >  2:5096093/1‑150 (MQ=255)
   tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                   >  1:1636865/1‑150 (MQ=255)
     cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACCATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                                                                                 >  1:5587343/1‑150 (MQ=255)
      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAGAGATTTGGCAGACGCTGTCTGc                                                                                                                                                >  1:135492/1‑150 (MQ=255)
      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                                <  1:2745589/150‑1 (MQ=255)
      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTACCATCGGCAAAGATTTGGCAGACAGCGTCTGc                                                                                                                                                <  2:4117665/150‑1 (MQ=255)
      aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTACAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                                <  1:6869676/150‑1 (MQ=255)
       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGTAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                                               <  1:1028804/150‑1 (MQ=255)
       aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGACTTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATGTGGCCGACGCTGTCTGcc                                                                                                                                               >  2:7277247/1‑150 (MQ=255)
        aaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGATTTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCa                                                                                                                                              <  1:5279087/150‑1 (MQ=255)
          aaCTTCCGCCACATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                                                            <  1:6346304/150‑1 (MQ=255)
          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                                                            <  1:4426882/150‑1 (MQ=255)
          aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                                                            <  1:1224428/150‑1 (MQ=255)
           aCTTACGCCAATTGCGGCTTTTTTCCCACGTTTTTCAACATGTTGAAATTCACCAGACACTGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAt                                                                                                                                           <  1:7307652/150‑1 (MQ=255)
           aCTTACGCCAAATGAGTCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCTTTGGGATTTCCGCAGACACCGTCTGCATATTTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCAAAt                                                                                                                                           <  1:903090/150‑1 (MQ=255)
            cTTACGCCATATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATc                                                                                                                                          <  2:2916579/150‑1 (MQ=255)
             ttccGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCt                                                                                                                                         <  2:8206177/147‑1 (MQ=255)
             ttACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCACATCt                                                                                                                                         >  2:5257095/1‑150 (MQ=255)
              tACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCTTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCAAATCtt                                                                                                                                        <  1:1971379/150‑1 (MQ=255)
                 gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCCAATCTTTcc                                                                                                                                     >  2:5562729/1‑150 (MQ=255)
                 cccATATGAGGTTTTTTTACCTCTTTTTTCAACATGTTTAATTTCACCAGACAATGTGTGGCTAATTAACACCATTGGGATTTCCGCAGCCACCGTCGGCAGAATTCCCTTCGGCAAAGATTTGGCTGACGCTGTCTGCCAAATCTTTcc                                                                                                                                     <  1:5252721/149‑1 (MQ=255)
                  ccAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTccc                                                                                                                                    >  1:3148520/1‑150 (MQ=255)
                   cAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTcccc                                                                                                                                   >  1:3883151/1‑150 (MQ=255)
                     aaTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCCAAACTTTccccat                                                                                                                                 >  1:7078813/1‑148 (MQ=255)
                      aTGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTg                                                                                                                                <  2:3318058/150‑1 (MQ=255)
                       tGAGGCTTTTTTACCTATTTTTACAACTTGTTTAAATTCACCAGACAATGTCTGGCTAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCATAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGc                                                                                                                               <  1:7732975/150‑1 (MQ=255)
                           gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                                                           >  2:6355340/1‑150 (MQ=255)
                            cTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTTCCCAgg                                                                                                                          >  1:5875864/1‑150 (MQ=255)
                             ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                                                         >  1:5063381/1‑150 (MQ=255)
                             ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                                                         >  1:8109378/1‑150 (MQ=255)
                                 ttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCAc                                                                                                                     >  1:4125893/1‑150 (MQ=255)
                                     ctctTTTTTCAACATGTTGTAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                                 <  1:6833049/150‑1 (MQ=255)
                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTAc                                                                                                                 >  1:3439608/1‑150 (MQ=255)
                                     ctctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTAc                                                                                                                 >  2:1906115/1‑150 (MQ=255)
                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                                >  2:5320602/1‑150 (MQ=255)
                                      tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                                >  2:3122507/1‑150 (MQ=255)
                                              cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGtttc                                                                                                        >  2:1009084/1‑149 (MQ=255)
                                                  aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACttt                                                                                                    <  2:1493018/150‑1 (MQ=255)
                                                  aTGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCGGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACttt                                                                                                    <  1:4041728/150‑1 (MQ=255)
                                                    gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                  <  2:7171996/150‑1 (MQ=255)
                                                     ttGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCg                                                                                                 >  2:3655270/1‑150 (MQ=255)
                                                      tGAAATTCACCAGACAATGTCTGGCGCATTAACACCAGTGGGAAGTCCGCAGACACCGTCTGCAGAAGTCCCATCGGCACAGATGTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGt                                                                                                <  2:4898099/150‑1 (MQ=255)
                                                      gttaaTTCGCCAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGt                                                                                                >  1:2482038/4‑150 (MQ=255)
                                                        aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                                                              <  2:374327/150‑1 (MQ=255)
                                                        aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                                                              <  2:7847522/150‑1 (MQ=255)
                                                        aaaTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTa                                                                                              >  2:7991573/1‑150 (MQ=255)
                                                          aTTCACCAGACAATGTCTGGCTAATTAACACCTTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                            <  2:7353395/150‑1 (MQ=255)
                                                            tcgccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaa                                                                                          >  1:7244119/4‑150 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                       >  2:772019/1‑150 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                       <  2:3594515/150‑1 (MQ=255)
                                                               ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                       >  2:690176/1‑150 (MQ=255)
                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                                     >  2:3140459/1‑150 (MQ=255)
                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                                     >  2:1266367/1‑150 (MQ=255)
                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                                     >  2:503937/1‑150 (MQ=255)
                                                                 aGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTg                                                                                     >  2:2734269/1‑150 (MQ=255)
                                                                       tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                               <  2:1384962/150‑1 (MQ=255)
                                                                           tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTATACTTTCCGTTAAAAACGCTGACGCCCGCAg                                                                           >  2:6114883/1‑150 (MQ=255)
                                                                            ggCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCAACGTCCGTTTACTTTCCGTTAAAAAAGCTGAAGCCCGCAGc                                                                          >  1:234643/1‑150 (MQ=255)
                                                                             gCGAATTAACACCATTGGGAATTACGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCt                                                                         >  2:66476/1‑150 (MQ=255)
                                                                                   tAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATg                                                                   <  2:2096984/150‑1 (MQ=255)
                                                                                      cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                                <  1:3245378/150‑1 (MQ=255)
                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCTGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                                                              <  1:2134191/150‑1 (MQ=255)
                                                                                        ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                                                              <  1:3864071/150‑1 (MQ=255)
                                                                                         cATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAag                                                             <  2:3464928/150‑1 (MQ=255)
                                                                                          aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                            <  2:2853358/150‑1 (MQ=255)
                                                                                               gAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCAGTCTGCCCAATATTTCCCCATGCCCAGGTCCACCAACCGCCGGTTAATTTACGTTTAAAAAGCTGACGCCCGCCCCGTTTTTGAAAAAGAGAccc                                                       >  1:6511439/1‑148 (MQ=255)
                                                                                                atgtCCGCAGACACCGTCTGCATAATACCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCt                                                      <  1:3959657/147‑1 (MQ=255)
                                                                                                 aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTGTGCCCAAACTTTACCCATGCCCAGGTCCACCAACGTCCGTTTACTTTGCGGTAAAAAAGCGGTAGCCCCCAGCGTTTTTGTAAAAGAGAAGcgc                                                     >  1:6235770/1‑148 (MQ=255)
                                                                                                  ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAACGTTCCCCTTGCCCCGGTCCACCAACGTCCGGTTAAGTTCCGTTAAAAAAGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCAcc                                                    >  1:7898973/1‑150 (MQ=255)
                                                                                                    ccGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTACTTTGCGTTAAAAACGCTGACGCCCGCCGCTGTTATGGAAAATAGTCGCTCCGc                                                  >  1:6587300/1‑150 (MQ=255)
                                                                                                     cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTACCCATTCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCt                                                 >  1:6000608/1‑150 (MQ=255)
                                                                                                         gACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                             <  1:7541550/150‑1 (MQ=255)
                                                                                                           cacCGTCTGCAGAATTCCCATCGGCAAAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTGGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt                                           <  1:1124410/150‑1 (MQ=255)
                                                                                                           cacCGTCTGCAGAATTCCCATCGGCAAAGATTGGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt                                           <  1:3629497/150‑1 (MQ=255)
                                                                                                              cGTCTGCAGAATTCCCATCGGCAATGATTTTGCAGACGCTGTCTGCCAAAAATTTCCCCATTCCCAGGTCCACCAAAGTCCGTTTAAATTGCGTTAAAAAAGCGGAAGCCCGCCGCATTTATGAAAAAGAGACGCTACCCTGTGGCTGGt                                        >  1:4637829/1‑150 (MQ=255)
                                                                                                                tCTGCAGAATTCCCATCGGCAAAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCt                                      <  1:3118/150‑1 (MQ=255)
                                                                                                                    cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTGTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                                  <  1:1456790/150‑1 (MQ=255)
                                                                                                                       aaTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGcc                               >  2:8170523/1‑150 (MQ=255)
                                                                                                                           cccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCt                           <  1:104443/150‑1 (MQ=255)
                                                                                                                            ccATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTa                          >  2:576577/1‑150 (MQ=255)
                                                                                                                             cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                         >  2:6490744/1‑150 (MQ=255)
                                                                                                                              aTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTaga                        >  2:4373584/1‑150 (MQ=255)
                                                                                                                                cGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                      <  2:177800/150‑1 (MQ=255)
                                                                                                                                cGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGc                      <  2:4594416/150‑1 (MQ=255)
                                                                                                                                 ggCAACGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCg                     <  1:1779487/150‑1 (MQ=255)
                                                                                                                                 ggCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCg                     <  2:3945541/150‑1 (MQ=255)
                                                                                                                                  gCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCgg                    >  2:2616583/1‑150 (MQ=255)
                                                                                                                                     aaGATTTGGCAGACAGCGTCTGCCAAATATTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAATTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATTAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGggcggcca                 >  1:2899633/1‑148 (MQ=255)
                                                                                                                                       gATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAt               >  2:4750922/1‑150 (MQ=255)
                                                                                                                                         tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg             <  2:135492/150‑1 (MQ=255)
                                                                                                                                         tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg             <  2:2512327/150‑1 (MQ=255)
                                                                                                                                         tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTACGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCGGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg             >  1:5771834/1‑150 (MQ=255)
                                                                                                                                           tGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg           <  1:3793290/150‑1 (MQ=255)
                                                                                                                                           tGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg           >  2:6002696/1‑150 (MQ=255)
                                                                                                                                           tGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg           >  2:5645440/1‑150 (MQ=255)
                                                                                                                                           tGGCAGACAGCGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg           >  2:1065874/1‑150 (MQ=255)
                                                                                                                                            ggCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCACCTTGCGa          >  2:6170420/1‑150 (MQ=255)
                                                                                                                                            ggCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGa          <  1:4858362/150‑1 (MQ=255)
                                                                                                                                               aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc       >  1:5175649/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTT  >  NC_000913/3368072‑3368369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: