Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 6.2% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 6.2% 862.9 / 8.1 338intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (104/204);  new base G (14/15);  total (119/219)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 344 total aligned reads displayed.

ACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504707‑3505004
                                                                                                                                                    |                                                                                                                                                     
aCAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTAAACTATTGATAATAAAAGACATTAACAGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGtt                                                                                                                                                      >  1:3192819/1‑150 (MQ=255)
 cAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTc                                                                                                                                                     <  2:1622726/150‑1 (MQ=255)
  aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  2:7241146/1‑150 (MQ=255)
   aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                   <  1:1811635/150‑1 (MQ=255)
   aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                   <  2:4587301/150‑1 (MQ=255)
      cGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAACGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTc                                                                                                                                                <  2:538840/150‑1 (MQ=255)
       ggTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTCTTGATAATAAAAGACATTAACCGTAGGCGGGTTAAGTTGCGCCAGCATCGCATCCGGCGATGCGGGCGCGGTGAATTTTACATCCCGTACGGTccccgcc                                                                                                                                               >  2:1736531/1‑147 (MQ=255)
        gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  2:692329/150‑1 (MQ=255)
         tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                             >  1:957851/1‑150 (MQ=255)
         tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                             <  1:213130/150‑1 (MQ=255)
           cACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACAccca                                                                                                                                           >  1:7608720/1‑149 (MQ=255)
                ctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAAccc                                                                                                                                      >  1:2318272/1‑150 (MQ=255)
                 tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                     <  2:6627305/150‑1 (MQ=255)
                 tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                     <  2:1920966/150‑1 (MQ=255)
                  ccGAACTCTTCAGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAACACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctc                                                                                                                                    <  1:2583579/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAAcccacac                                                                                                                                  >  1:5005276/1‑146 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:2710634/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:328848/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  2:4766526/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:1685076/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                  <  1:4506609/150‑1 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCtctc                                                                                                                                  >  1:1012141/1‑150 (MQ=255)
                    gAACTCTTCTGCCGCGCCAATCGCATCACATTAACTATTTATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGACAGCATCTCATCGGGCTATGCTGGCGCGTTGATTTTTACATCCCGTACGTTCCCCTCACCCTAACCCtatc                                                                                                                                  <  2:405833/150‑1 (MQ=255)
                      aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                <  2:1654240/150‑1 (MQ=255)
                      aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                <  1:6205124/150‑1 (MQ=255)
                        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                              >  1:1232336/1‑150 (MQ=255)
                        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCAAACCCTCTCCCCa                                                                                                                              >  1:8133486/1‑150 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  2:5994685/150‑1 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            >  2:1276503/1‑150 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  1:6903355/150‑1 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCGCTCCCCaaa                                                                                                                            >  2:5106523/1‑150 (MQ=255)
                              gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCACCCCAAAgggg                                                                                                                        >  1:4053951/1‑150 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:1539832/150‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTACCCAAAGGGGCgg                                                                                                                     >  1:8058189/1‑149 (MQ=255)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  1:6135089/150‑1 (MQ=255)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGAGGCCCGCACCCGAAACCACTACCCCAAGGGGCGgggg                                                                                                                  >  1:5646905/1‑150 (MQ=37)
                                     cAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgggg                                                                                                                 <  2:2954322/150‑1 (MQ=255)
                                      aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCACCAAAACCACAACCCCAAGGGGCGAGGGGa                                                                                                                >  2:2927215/1‑150 (MQ=38)
                                      aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  1:4945550/150‑1 (MQ=255)
                                       aTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCAACCTAACCCACTCCCCCAAGGGGCGAGGGGAc                                                                                                               >  1:5860874/1‑150 (MQ=255)
                                            aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                          <  1:3644064/150‑1 (MQ=255)
                                             tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCa                                                                                                         >  2:6180865/1‑150 (MQ=255)
                                             tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:1968343/150‑1 (MQ=255)
                                             tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  2:7080297/150‑1 (MQ=255)
                                               aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGt                                                                                                       >  2:1354638/1‑150 (MQ=255)
                                                 cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                     <  2:7875911/150‑1 (MQ=255)
                                                  ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                    <  2:109099/150‑1 (MQ=255)
                                                     aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                 <  1:7225490/150‑1 (MQ=255)
                                                       tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                               <  2:552608/150‑1 (MQ=255)
                                                       tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                               <  2:8043862/150‑1 (MQ=255)
                                                         ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                             <  1:1794871/150‑1 (MQ=255)
                                                         ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCTAAACCTCACCCCAAAGGGGCGCGGGGTCCGTTCAGTACGGCATTTa                                                                                             >  1:679195/1‑150 (MQ=38)
                                                          tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                            <  1:1308123/150‑1 (MQ=255)
                                                          tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                            <  1:5235171/150‑1 (MQ=255)
                                                           gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                           <  2:3707831/150‑1 (MQ=255)
                                                            ataataAAAGACATTAACCGTAGGCCGGGTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                          <  2:4673088/150‑1 (MQ=255)
                                                               ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACACCCCAAAGGGGCGTGGGGACCCTTCAGTAACGAAATTAGCttta                                                                                       >  2:2470241/1‑148 (MQ=38)
                                                                   aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                   <  1:4317830/150‑1 (MQ=255)
                                                                     gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTGAGCTTGACGGTGc                                                                                 <  2:2145584/150‑1 (MQ=255)
                                                                         ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCCAAACCACACCCCCAAGGGGCGCCGGGCCCCGTCAGTACGGTAATTAGCGTGAGGGTgggatc                                                                             >  2:7645209/1‑145 (MQ=25)
                                                                          gaaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                            <  2:7824168/149‑1 (MQ=255)
                                                                                   gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                   <  1:1516393/150‑1 (MQ=255)
                                                                                       gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCGCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                               >  2:5835066/1‑150 (MQ=255)
                                                                                       gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:3126064/150‑1 (MQ=255)
                                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:3790266/150‑1 (MQ=255)
                                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:7145471/150‑1 (MQ=255)
                                                                                        aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:4711961/150‑1 (MQ=255)
                                                                                         ttcaacgcgcCAGCATCGCCGGATGCGATGCTGGCGCATTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             >  2:8232615/7‑150 (MQ=38)
                                                                                         tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:5688853/150‑1 (MQ=255)
                                                                                         tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  1:456974/150‑1 (MQ=255)
                                                                                         tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  1:4319837/150‑1 (MQ=255)
                                                                                         tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:6956307/150‑1 (MQ=255)
                                                                                          aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                            <  2:5294970/150‑1 (MQ=255)
                                                                                            gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  1:2503741/150‑1 (MQ=255)
                                                                                            gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTTGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          >  2:3351956/1‑150 (MQ=255)
                                                                                             aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAt                                                         <  1:2220997/150‑1 (MQ=255)
                                                                                             aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAt                                                         <  1:7390051/150‑1 (MQ=255)
                                                                                               gcgcCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAc                                                       <  2:6479062/150‑1 (MQ=255)
                                                                                                 gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActc                                                     <  1:7683904/150‑1 (MQ=255)
                                                                                                 gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCGCACCCCCAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAGTAAATACTGGCAATActc                                                     >  2:7428620/1‑150 (MQ=255)
                                                                                                   cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                   <  2:5945793/150‑1 (MQ=255)
                                                                                                    aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  2:6710657/150‑1 (MQ=255)
                                                                                                    aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCAAACCCACTCCCCCAAGGGGCGCGGGGCCCGGTCAGGCCGGCATTTAGCTTGTCCGGGCAATCCAGCATGAAATACAGGCAAGAcgcgac                                                  >  2:4194116/1‑145 (MQ=255)
                                                                                                    aGCATCGCATCCGGCGATGCAGGCGCGAAGAATTTAACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAACGGGCGAGGGGACCGTACAGTACGGAATTTAGCTTGACGGAGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  2:6339527/150‑1 (MQ=38)
                                                                                                     gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                 <  1:7951214/150‑1 (MQ=255)
                                                                                                         cgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTAACGCaa                                             >  2:578234/1‑150 (MQ=255)
                                                                                                           catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                                           <  2:6784337/150‑1 (MQ=255)
                                                                                                           catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCAAAGGGGCGAGGGGACCCGTCAGTACGGAATGTAGCTTGTAGGGGCTAACCACCAGTAAAAAAAGGCCATACTCTCACGCcaaa                                           >  2:2558028/1‑150 (MQ=255)
                                                                                                               cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  1:5504183/150‑1 (MQ=255)
                                                                                                                ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAACCCAAAACCCAAAGGGGCGCGGGGACCCGTCACTAACGAATTTAGCATTAAGGTGCTAAACACCAGTAAATAAAGGCAAAAAACACAAGCaaaaaaagc                                      >  2:1597557/1‑145 (MQ=255)
                                                                                                                ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCCTTAAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGCAAAAAACTCACGCAAAAACCgc                                      >  2:435499/1‑150 (MQ=255)
                                                                                                                  cGATGCTGGCGCGTTGATTTTTACCTCCCGTTCGTTCCCCTCACCCTAACCCTCTCCCCCACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAATTAGTGGCAATACTCTCACGCAAAATCCgcag                                    <  1:6529382/150‑1 (MQ=38)
                                                                                                                  cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTATCTTGACGGTGCTATCCAGCATTAAATACAGGCAATACTCACAAGCAAAAAACgcag                                    >  2:5922183/1‑150 (MQ=255)
                                                                                                                      gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                <  1:2282522/150‑1 (MQ=255)
                                                                                                                             cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACTCACGCAAAATACGCAGCACCGtctcat                         >  2:4987402/1‑148 (MQ=255)
                                                                                                                                           tCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc           <  2:2660648/150‑1 (MQ=255)
                                                                                                                                             ccGTACGTTCCCCTCCCCCGCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg         <  2:2262576/150‑1 (MQ=255)
                                                                                                                                               gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt       >  2:3991075/1‑150 (MQ=255)
                                                                                                                                                   gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  2:2388893/150‑1 (MQ=255)
                                                                                                                                                   gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   >  2:2728932/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504707‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: