Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,626 T→A 5.9% K150N (AAA→AAT malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,6260TA 5.9% 1725.9 / 8.0 552K150N (AAA→AATmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (206/289);  new base A (51/2);  total (261/291)
Fisher's exact test for biased strand distribution p-value = 6.75e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 564 total aligned reads displayed.

GCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244479‑4244775
                                                                                                                                                   |                                                                                                                                                     
gCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAttt                                                                                                                                                     <  2:30899/150‑1 (MQ=255)
  tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaa                                                                                                                                                   >  2:5163259/1‑150 (MQ=255)
  tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTTCAGTTTTTTCTCGCCGCCAAAATTaa                                                                                                                                                   >  2:3590559/1‑150 (MQ=255)
   catcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                                                  <  2:2632249/150‑1 (MQ=255)
   catcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaa                                                                                                                                                  >  2:2356248/1‑150 (MQ=255)
    atcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                                                 >  2:5905408/1‑150 (MQ=255)
    atcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGTTTTTGCTCGCCGCCAAATTTaaaa                                                                                                                                                 >  2:1439943/1‑150 (MQ=255)
     tcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAg                                                                                                                                                <  1:2238837/150‑1 (MQ=255)
      catcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGc                                                                                                                                               <  2:8018433/150‑1 (MQ=255)
       atcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGGTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGCg                                                                                                                                              >  2:3247552/1‑150 (MQ=255)
        tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCCAAATTAAAAGCGt                                                                                                                                             >  1:5719225/1‑150 (MQ=255)
        tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGt                                                                                                                                             >  1:8244051/1‑150 (MQ=255)
        tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGt                                                                                                                                             >  1:6562816/1‑150 (MQ=255)
          aCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCAAATTTAAAAGCGTCg                                                                                                                                           >  2:6433788/1‑150 (MQ=255)
          aCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCg                                                                                                                                           >  1:5943412/1‑150 (MQ=255)
            tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCAAATTTAAAAGCGTCGga                                                                                                                                         >  1:8100128/1‑150 (MQ=255)
              tgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGgaga                                                                                                                                       <  1:6683403/150‑1 (MQ=255)
                ttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGGTGCAGGTTTTGCGCGCCGCCCCCATTAAAAGCGGCGGAGAgg                                                                                                                                     >  1:1255075/1‑150 (MQ=255)
                ttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCGTTCAGGTGCAGTGTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAgg                                                                                                                                     >  2:5673252/1‑150 (MQ=255)
                 tgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGt                                                                                                                                    >  2:1517938/1‑150 (MQ=255)
                 tgCCATCCGGCAGAATGGCGGTAATGTCAATCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTGAAGTTTTTTGACTCAGCCAAATTTAAAATAGTAGGagagtt                                                                                                                                    >  1:1044624/1‑148 (MQ=255)
                   ccATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTGTGAGTTGCAGTTTGTGCTCGCCGCCAAATTTTAAGGCGTCGGTGAGGTag                                                                                                                                  >  2:6270661/1‑149 (MQ=255)
                   ccATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGTGGCAGTTGTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGTaa                                                                                                                                  >  2:2516866/1‑150 (MQ=255)
                     aTCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAtt                                                                                                                                >  2:5468347/1‑150 (MQ=255)
                     aTCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCATTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAtt                                                                                                                                >  2:2781578/1‑150 (MQ=255)
                      tCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGAAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttt                                                                                                                               >  2:6030210/1‑150 (MQ=255)
                       ccGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAtttt                                                                                                                              <  2:5066377/150‑1 (MQ=255)
                        cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttttt                                                                                                                             <  2:582039/150‑1 (MQ=255)
                        cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttttt                                                                                                                             <  1:3647699/150‑1 (MQ=255)
                        cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttttt                                                                                                                             >  2:3474199/1‑150 (MQ=255)
                         ggCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTg                                                                                                                            >  2:2055299/1‑150 (MQ=255)
                          gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           <  1:3037480/150‑1 (MQ=255)
                          gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           <  1:6180400/150‑1 (MQ=255)
                            aGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCg                                                                                                                         <  2:2914653/150‑1 (MQ=255)
                             gAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCgg                                                                                                                        <  1:6806279/150‑1 (MQ=255)
                               aTGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGtt                                                                                                                      <  2:2127418/150‑1 (MQ=255)
                                  gCGGTAATGTAACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTAGCCGCCAAATTTAAAAGCCTCGGAGAGGTAAATTTTGCCGGTTTCg                                                                                                                   >  2:1124582/1‑150 (MQ=255)
                                   cGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCTGTTGCTGTTTTTGCTCGCCTCCAAATTTAAAAGCGTCGGAGAGGTTATTTTTGCCGGTTTCGc                                                                                                                  <  1:2914107/150‑1 (MQ=255)
                                   cGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGc                                                                                                                  <  2:4473220/150‑1 (MQ=255)
                                        aTGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTGAGTTGGAGTTTTTTCTCGCCGCCCAATTTAAAATCGTCGGAGGGGTAATTTTTTCCGGTTTCGCCGTCg                                                                                                             >  1:4124459/1‑150 (MQ=255)
                                          gTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTTAGTTTGAGTTTTTGGTGGGCGCCCAATTTTAAATAGTGGGTGAGGTAATTTTTTGGGGTTTGGGCGTCGcg                                                                                                           >  1:1706208/1‑149 (MQ=255)
                                          gTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTAAGTTTCAGTTTTTTCTAGACGCCACATTTAAAATCGTAGGAGAGGTAATTTTTGGCCGTTTAGCCGTCGcg                                                                                                           >  1:5233073/1‑149 (MQ=255)
                                             aCTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTGTTTGCTCGCCCCCACAATTAAAACCGGCGGAGAGGTAATTTTTTCCGGGTTCGCCGGCCCTGAg                                                                                                        >  1:7087224/1‑150 (MQ=255)
                                                 aGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGGAGTTTTTTGTCGCCGCCAAATTTAAAAGCGTCGGTGTGGTAATTTTTGGCGGTTTCGCCGTCGCTGTGCGcc                                                                                                    >  2:501689/1‑150 (MQ=255)
                                                  gcgcCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                                                   <  2:8229887/150‑1 (MQ=255)
                                                  gcgcCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCa                                                                                                   <  1:5426650/150‑1 (MQ=255)
                                                       tGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGc                                                                                              >  2:2689760/1‑150 (MQ=255)
                                                       tGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTTGGGTTTTTTGTAGCCGCCAAAATTAAAAGCGTGGGGGAGGTAAATTTTTGCGGTTTAGCCGGCGCATAGCGcccggttc                                                                                              >  1:7697384/1‑144 (MQ=255)
                                                           gATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGGAGGTTTTTGTCGCCGCCCAAATTAAAATCGTGGGAGAGGTAATTTTTTGCGGGTTGGCCGGCGCGGAGCGCCCGtttgcaca                                                                                          >  1:4929621/1‑144 (MQ=255)
                                                               cTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATc                                                                                      <  2:6596120/150‑1 (MQ=255)
                                                                       tCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTTCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCccgccg                                                                              >  2:1607686/1‑150 (MQ=255)
                                                                               aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                                                      <  2:6500656/150‑1 (MQ=255)
                                                                                 aTTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                                                    <  1:7218604/150‑1 (MQ=255)
                                                                                    cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                                                                 <  1:6883489/150‑1 (MQ=255)
                                                                                    cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                                                                 <  1:7323659/150‑1 (MQ=255)
                                                                                    cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                                                                 <  2:7014279/150‑1 (MQ=255)
                                                                                    cgcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCaaa                                                                 <  2:7886059/150‑1 (MQ=255)
                                                                                     gcgcgTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGGTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACACATCGCCCGCCGGGTAAAGACCAAAg                                                                >  2:1897686/1‑150 (MQ=255)
                                                                                                 tCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTg                                                    <  1:3987313/150‑1 (MQ=255)
                                                                                                  cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  1:1777640/150‑1 (MQ=255)
                                                                                                     gCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTTCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTTCCGGGTTAGCCGTCGCTGAGCGCCAGTTTCCACTCATAGCCCGCCGGGTAAAGAACAAAGTTATATGtttttacag                                                >  2:1842633/1‑145 (MQ=255)
                                                                                                       tGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTAGCCCCCAAATTTAAAAGCGTAGGAGAGGTAAATTTTTCCCGTTTAGCCCTCGCTGAGCGCCAGTTTCCACAAAACGCCCGCCCGGTAAAAAACAAAGTTATAGGTTTTTCCAGCt                                              >  2:3821092/1‑150 (MQ=255)
                                                                                                        gggCGGTCGTTTATTTCAGTTGCAGTTTTTTCTAGCCCCCAAATTTAAAAGCGTCGGAGAGGTAAATTTTTCCGGTTTCGCCCTCGCTGAGCGCCACTTTCCACTCATCGCCCCCCCGGTAAATACCAAAGTTATAGGTTTTTCCAGCtt                                             >  2:1007407/1‑150 (MQ=255)
                                                                                                            ggTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCa                                         <  1:7936023/150‑1 (MQ=255)
                                                                                                               cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  1:7386421/150‑1 (MQ=255)
                                                                                                               cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  1:2601801/150‑1 (MQ=255)
                                                                                                               cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  2:7809620/150‑1 (MQ=255)
                                                                                                                gTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAg                                     >  1:1902867/1‑150 (MQ=255)
                                                                                                                gTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAg                                     >  2:3683411/1‑150 (MQ=255)
                                                                                                                  ttctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCg                                   <  2:1703332/150‑1 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:3177981/1‑150 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  1:423035/1‑150 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:4636039/1‑150 (MQ=255)
                                                                                                                     tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                <  2:4827824/150‑1 (MQ=255)
                                                                                                                      ttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCt                               >  1:6013806/1‑150 (MQ=255)
                                                                                                                       tcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTCTAGGTTTTGCCTGCTTGCCAGGAGCGATCTa                              >  1:2154425/1‑150 (MQ=255)
                                                                                                                        cAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaa                             <  1:482747/150‑1 (MQ=255)
                                                                                                                         aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaac                            <  1:373516/150‑1 (MQ=255)
                                                                                                                           ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          <  1:703117/150‑1 (MQ=255)
                                                                                                                           ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          <  1:3323846/150‑1 (MQ=255)
                                                                                                                            tGCAGTTGTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaac                         >  1:3530658/1‑150 (MQ=255)
                                                                                                                             gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca                        <  1:6250248/150‑1 (MQ=255)
                                                                                                                              cAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAacac                       <  2:430119/150‑1 (MQ=255)
                                                                                                                                   tttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCt                  >  2:4099763/1‑150 (MQ=255)
                                                                                                                                     tGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgc                <  1:2486817/150‑1 (MQ=255)
                                                                                                                                     tGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgc                <  1:5060105/150‑1 (MQ=255)
                                                                                                                                       cTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgcgc              >  2:7495744/1‑150 (MQ=255)
                                                                                                                                        tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCgg                                                                             <  2:7441852/86‑1 (MQ=255)
                                                                                                                                        tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             >  2:6721441/1‑150 (MQ=255)
                                                                                                                                        tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             <  2:762645/150‑1 (MQ=255)
                                                                                                                                           ccgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGt          <  1:1558060/150‑1 (MQ=255)
                                                                                                                                           ccgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGt          <  2:1274220/150‑1 (MQ=255)
                                                                                                                                           ccgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGt          <  1:6382069/150‑1 (MQ=255)
                                                                                                                                              ccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCa       >  2:5299854/1‑150 (MQ=255)
                                                                                                                                               cAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaa      >  1:4610701/1‑150 (MQ=255)
                                                                                                                                               cAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaa      <  1:3427612/150‑1 (MQ=255)
                                                                                                                                                aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaa     >  2:726357/1‑150 (MQ=255)
                                                                                                                                                 aaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaaa    >  1:2791757/1‑150 (MQ=255)
                                                                                                                                                 aaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaaa    <  1:7475971/150‑1 (MQ=255)
                                                                                                                                                   tttAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGt  >  2:1823322/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244479‑4244775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: