Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,083 A→G 7.7% intergenic (+481/+133) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0830AG 7.7% 521.3 / 26.6 183intergenic (+481/+133)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base A (97/71);  new base G (3/11);  total (101/82)
Fisher's exact test for biased strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 184 total aligned reads displayed.

ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269935‑3270228
                                                                                                                                                    |                                                                                                                                                 
atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCCTTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                                                  >  1:3288657/1‑150 (MQ=255)
  cattCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAGCTGACAGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCCGTCCTCTTTCCGCCCCTCCAAAATCTAATACCCGCCGAcgcg                                                                                                                                                >  2:2636764/3‑150 (MQ=11)
   tttCTGCCCCTCCAAACTTAAAAACCCGCCGATGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTGTTTTGGGTTTTGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  1:1581536/150‑1 (MQ=11)
   tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  2:1451099/150‑1 (MQ=37)
     tCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                                                                                                             >  1:1357047/1‑150 (MQ=37)
       tGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttt                                                                                                                                           <  2:2683397/150‑1 (MQ=11)
       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGTGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttt                                                                                                                                           <  1:1644696/150‑1 (MQ=11)
       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttt                                                                                                                                           <  2:1158076/150‑1 (MQ=255)
       tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttt                                                                                                                                           <  2:316861/150‑1 (MQ=255)
       ggCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttt                                                                                                                                           <  1:703700/149‑1 (MQ=255)
        gCCCCTCCAACAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                                                                          <  2:358813/150‑1 (MQ=11)
         ccccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                         <  2:1348130/150‑1 (MQ=37)
            cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                                                                      >  2:2336634/1‑150 (MQ=37)
            cTCCAAAACTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgct                                                                                                                                      <  2:3894165/150‑4 (MQ=11)
             tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  2:3415208/1‑150 (MQ=21)
                 aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                                 >  1:2587884/1‑149 (MQ=37)
                       aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAGGCGGGTTTTTACGGAAAACAgggg                                                                                                                           >  2:2577327/1‑148 (MQ=11)
                       aaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTg                                                                                                                           >  2:2306728/1‑150 (MQ=17)
                               ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:770915/150‑1 (MQ=37)
                               ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  2:1818625/150‑1 (MQ=37)
                               ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  2:4196161/150‑1 (MQ=37)
                                 gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTATAGTGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCCCCGAACCGGGTATTTACGTAAACCAGGAGAAACTTAccc                                                                                                                 >  1:2258762/1‑149 (MQ=11)
                                     cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATaa                                                                                                             <  1:2910820/150‑1 (MQ=37)
                                     cGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGaaca                                                                                                             >  2:2186023/1‑147 (MQ=255)
                                       ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:1054081/1‑150 (MQ=17)
                                                 tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                 <  2:3059611/150‑1 (MQ=40)
                                                  acaaCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGTTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                <  1:2443874/148‑1 (MQ=255)
                                                  aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                <  1:408943/150‑1 (MQ=40)
                                                   aaaCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  1:3089679/150‑1 (MQ=255)
                                                   aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  1:452255/150‑1 (MQ=39)
                                                   aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  2:3546863/150‑1 (MQ=39)
                                                     aCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                             <  1:1044421/150‑1 (MQ=38)
                                                              aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                                                    <  1:2289406/150‑1 (MQ=37)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                  <  2:1918425/150‑1 (MQ=37)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                  <  2:1815216/150‑1 (MQ=37)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGTAGAGcgtcgt                                                                                  >  2:19093/1‑150 (MQ=255)
                                                                tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATATTTCTTCCCCGCCAAAAGCAAAAAACCCCCCAACCGGGTTTTTACGTAAAACAGGTGAAACTGACCCATAAGCCCCGTTCTTGTGGGTATAGcgtcgt                                                                                  >  1:1613399/1‑150 (MQ=11)
                                                                    cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGTCGTGGaa                                                                              >  2:3620371/1‑149 (MQ=12)
                                                                          gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcat                                                                        >  2:3011302/1‑150 (MQ=37)
                                                                           ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               >  2:3513548/1‑78 (MQ=34)
                                                                           ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTATGCCCCTCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAATCCGCTTTCTTTTGGGTATAGCGTAGTAGAAAGtcaat                                                                       >  2:3827228/1‑148 (MQ=11)
                                                                              ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                                                    <  1:3660376/150‑1 (MQ=37)
                                                                                          tagaGTGTCGTGGACACGCATTCATCTTTCTGCCCCTCCAACAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCt                                                        <  1:1023991/147‑1 (MQ=11)
                                                                                            taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                      <  1:2782008/150‑1 (MQ=37)
                                                                                                gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                  <  2:2896459/150‑1 (MQ=37)
                                                                                                gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                  <  1:3011302/150‑1 (MQ=37)
                                                                                                   gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCa                                               >  2:328206/1‑150 (MQ=255)
                                                                                                   gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATAGATCTATATGGGCGTCCAAAAGCa                                               >  2:3537121/1‑150 (MQ=11)
                                                                                                    tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                              >  1:718434/1‑150 (MQ=37)
                                                                                                      gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaa                                            <  2:4131426/150‑1 (MQ=37)
                                                                                                       aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGTCCCTCCAAAAGCaaaaa                                           <  2:1638981/150‑1 (MQ=37)
                                                                                                       aCAGTCATTCATCATTCTGCCACTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                           <  2:873279/150‑1 (MQ=255)
                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTGTCTGCCCCTCCAAAAGCAAAAAcc                                         >  1:3768570/1‑150 (MQ=255)
                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                                                    <  2:2862853/139‑1 (MQ=37)
                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                         >  1:4102916/1‑150 (MQ=37)
                                                                                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAAcc                                         >  1:2147616/1‑150 (MQ=37)
                                                                                                           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       >  1:71222/1‑150 (MQ=37)
                                                                                                           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCGCCAAAAGCAAAAACccg                                       >  2:1496795/1‑150 (MQ=255)
                                                                                                            cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                      <  2:466333/150‑1 (MQ=37)
                                                                                                            cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                      <  1:4114820/150‑1 (MQ=37)
                                                                                                              ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                    >  1:985027/1‑150 (MQ=37)
                                                                                                               tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                   >  2:2246813/1‑150 (MQ=37)
                                                                                                                 atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                 >  2:1948748/1‑150 (MQ=37)
                                                                                                                  tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                >  1:3146678/1‑150 (MQ=37)
                                                                                                                   cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                               >  1:3434224/1‑150 (MQ=37)
                                                                                                                   cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                               >  2:1554897/1‑150 (MQ=37)
                                                                                                                   cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCAGTCATCGTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCg                               >  2:3284979/1‑150 (MQ=255)
                                                                                                                    tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                              <  1:184434/150‑1 (MQ=37)
                                                                                                                    tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                              >  1:2055129/1‑150 (MQ=37)
                                                                                                                       cgcgcccTCCACAAGCACAAACCCGCCGCATCGGGTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                           <  2:1647728/146‑1 (MQ=11)
                                                                                                                        tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                          <  2:3760678/150‑1 (MQ=37)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         <  2:2939398/150‑1 (MQ=37)
                                                                                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  2:4008179/1‑150 (MQ=37)
                                                                                                                          ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                        >  1:1339217/1‑150 (MQ=37)
                                                                                                                          ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGCAGCGGGttttt                        >  1:2521478/1‑150 (MQ=255)
                                                                                                                          ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAAACCGCCGAAGCGGGttttt                        >  1:397927/1‑150 (MQ=255)
                                                                                                                           cccTCCACCCGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                       <  2:4076740/150‑1 (MQ=255)
                                                                                                                           cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                       <  2:2373992/150‑1 (MQ=37)
                                                                                                                           cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                       >  2:2707308/1‑150 (MQ=37)
                                                                                                                           cacTCCACAACCAAAAACCCGCCGAAGCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                       <  2:4131312/148‑1 (MQ=255)
                                                                                                                            ccTCCAAAATCAAAAACCCGCCGTAGGGGTTTTTTACGTAAACCTGGTTAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGAAAAAACCCGCTTCGGCGGGtttttgc                      <  1:1395649/150‑3 (MQ=11)
                                                                                                                            ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      >  1:1712803/1‑150 (MQ=37)
                                                                                                                            ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTAc                      >  2:2144106/1‑150 (MQ=37)
                                                                                                                             ccccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgct                     <  1:435077/148‑4 (MQ=11)
                                                                                                                              tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACgg                    >  2:3302339/1‑149 (MQ=255)
                                                                                                                                tttacgtaaaaaCCCGCTTCGGCGGGTTTTTGAGTTAACCAGGTGAAACTGAACGATAAGCCGCTTTCTTTTGGGTATATAGGCGGGGACAGGCATTAATAATTCTGCCACTCCAAAATCAAAAACCCCCCGAAGCGGGTTTTTACGTaa                  >  1:2985421/8‑150 (MQ=2)
                                                                                                                                cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCACAAACCCGCCGCAGCGGGTTGTTACGTaa                  >  2:2774413/1‑150 (MQ=255)
                                                                                                                                cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                  >  2:2396994/1‑150 (MQ=37)
                                                                                                                                cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTaa                  >  1:2733395/1‑150 (MQ=255)
                                                                                                                                  caaGCAAAAACCCGCCGCAGCGGGTTTTTACGTAACCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                <  2:386508/149‑1 (MQ=255)
                                                                                                                                  aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAGATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTAAGTAAAt                >  2:493135/1‑150 (MQ=11)
                                                                                                                                     gtaaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAATCAg             >  2:1286481/3‑150 (MQ=11)
                                                                                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  2:3459653/1‑150 (MQ=37)
                                                                                                                                        aaatCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTGCAAAAGCAATATCCCGCCGAAGCGGGTTTTTAGGTTAATCAGGTg          >  2:3108589/1‑150 (MQ=11)
                                                                                                                                           aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa       >  1:336991/1‑150 (MQ=37)
                                                                                                                                            cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc      >  2:2588774/1‑150 (MQ=37)
                                                                                                                                              cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTGTGCCCCTACAAAATCAAAAACCCGCCGAAGCGGGTTTTTTAGTAAATAAGGTGtaaatt    >  2:3967550/1‑147 (MQ=11)
                                                                                                                                              cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGAAATCAGGTGAAACTg    >  2:517294/1‑150 (MQ=37)
                                                                                                                                               gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa   >  1:90784/1‑150 (MQ=37)
                                                                                                                                                ccgCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc  <  2:2822298/150‑1 (MQ=37)
                                                                                                                                                    |                                                                                                                                                 
ATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269935‑3270228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: